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The map label for this gene is hemK [H]

Identifier: 197104293

GI number: 197104293

Start: 913039

End: 913902

Strand: Reverse

Name: hemK [H]

Synonym: PHZ_c0827

Alternate gene names: 197104293

Gene position: 913902-913039 (Counterclockwise)

Preceding gene: 197104294

Following gene: 197104292

Centisome position: 22.87

GC content: 73.15

Gene sequence:

>864_bases
ATGGGCCTGAACCTCGTCCAGGCGTGGAGCCAGGCCAAGCAGCGCCTGGAGGCCGCCGGCCTCGCTGGCCCGGTGATCGA
CGCGCGCCTGCTGGTGGAAGCGGCCGCCGACGCCACCCGCGCCGACATCGTCACCGATCCCTACCGGCCGCTGACCCCCG
AGCAGGAGGCGAGACTGCAGGACTATCTGGCGCGGCGGGAGAAGCGCGAGCCGGTCAGCCACATCCTGGGCCGCAAGGGC
TTCTGGAAGATCATGCTGCGGGTGACGCCCGACGTGCTGACCCCGCGCCCAGACACCGAGACCGTCGTGGACGTGGCGCT
GAAGGCCTTCCCCGAGCACGCTCCGTGGTCGGTGCTGGACCTGGGTGTCGGCTCGGGCGCCATCCTGCTGGCGATCCTGG
CCGAGCGGCCGGCGGCCAAGGGCCTGGGGATCGACGCCTCGGAGGAAGCCCTGGCGGTGGCGCGCGACAACGCCGCGGCC
CTGGGCCTGGCCGGGCGGACGGCCCTGCTGCGCGGCGACTGGACGGCGGGCCTGGGGGACAGCGCCTTCGACCTGGTGGT
CTCGAACCCGCCCTACATCGCCAGCGACGTGCTGGAGACCCTCGAGCCCGAGGTGAAGGACTACGAGCCGCGGCTGGCCC
TGGAAGGCGGCGCCGACGGGCTGGACGCCTACCGCATCCTGGCCCCCGAGATCGTGCGCGTGCTGAAGCCCGGCGGGCGC
TTCGCGGTGGAGATCGGCTACGACCAGAAGGACGCCGTCGAGGCGCTGTTCCGCGCCGCGGGGGCCGAGGACGTGCGCAC
GATCCGCGACCTGGCCGACCGCGACCGGGTCGTCGCGGGTGCGAAGAAACCCCTTGGAAACTAG

Upstream 100 bases:

>100_bases
ACTGCGGAATCTATCTGAACGTCACGGCCGAGGGGGTCGTGAGCCTGGGCGATGCGGTGGCGGGCCCCGCCATCGAGGAC
GAACCCGCGAGGGCGCCGGA

Downstream 100 bases:

>100_bases
CACCTGACTCGCTACATCGTTGGATGTCTCCACCCAAATCGCCGGTGTTAAGGCAGACGCGTCCCGATGAAGGACGCCCG
CCAGCCCCGGCAGGCGCGAC

Product: modification methylase HemK

Products: NA

Alternate protein names: M.MloHemKP [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MGLNLVQAWSQAKQRLEAAGLAGPVIDARLLVEAAADATRADIVTDPYRPLTPEQEARLQDYLARREKREPVSHILGRKG
FWKIMLRVTPDVLTPRPDTETVVDVALKAFPEHAPWSVLDLGVGSGAILLAILAERPAAKGLGIDASEEALAVARDNAAA
LGLAGRTALLRGDWTAGLGDSAFDLVVSNPPYIASDVLETLEPEVKDYEPRLALEGGADGLDAYRILAPEIVRVLKPGGR
FAVEIGYDQKDAVEALFRAAGAEDVRTIRDLADRDRVVAGAKKPLGN

Sequences:

>Translated_287_residues
MGLNLVQAWSQAKQRLEAAGLAGPVIDARLLVEAAADATRADIVTDPYRPLTPEQEARLQDYLARREKREPVSHILGRKG
FWKIMLRVTPDVLTPRPDTETVVDVALKAFPEHAPWSVLDLGVGSGAILLAILAERPAAKGLGIDASEEALAVARDNAAA
LGLAGRTALLRGDWTAGLGDSAFDLVVSNPPYIASDVLETLEPEVKDYEPRLALEGGADGLDAYRILAPEIVRVLKPGGR
FAVEIGYDQKDAVEALFRAAGAEDVRTIRDLADRDRVVAGAKKPLGN
>Mature_286_residues
GLNLVQAWSQAKQRLEAAGLAGPVIDARLLVEAAADATRADIVTDPYRPLTPEQEARLQDYLARREKREPVSHILGRKGF
WKIMLRVTPDVLTPRPDTETVVDVALKAFPEHAPWSVLDLGVGSGAILLAILAERPAAKGLGIDASEEALAVARDNAAAL
GLAGRTALLRGDWTAGLGDSAFDLVVSNPPYIASDVLETLEPEVKDYEPRLALEGGADGLDAYRILAPEIVRVLKPGGRF
AVEIGYDQKDAVEALFRAAGAEDVRTIRDLADRDRVVAGAKKPLGN

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=293, Percent_Identity=29.6928327645051, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1787463, Length=275, Percent_Identity=36, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI87082085, Length=197, Percent_Identity=29.9492385786802, Blast_Score=86, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24582226, Length=283, Percent_Identity=30.3886925795053, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30749; Mature: 30618

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLNLVQAWSQAKQRLEAAGLAGPVIDARLLVEAAADATRADIVTDPYRPLTPEQEARLQ
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHH
DYLARREKREPVSHILGRKGFWKIMLRVTPDVLTPRPDTETVVDVALKAFPEHAPWSVLD
HHHHHHHHCCHHHHHHCCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHCCCCCCCEEEE
LGVGSGAILLAILAERPAAKGLGIDASEEALAVARDNAAALGLAGRTALLRGDWTAGLGD
ECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCEEECCCCEEEEECCCCCCCCC
SAFDLVVSNPPYIASDVLETLEPEVKDYEPRLALEGGADGLDAYRILAPEIVRVLKPGGR
CCEEEEECCCCHHHHHHHHHHCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCC
FAVEIGYDQKDAVEALFRAAGAEDVRTIRDLADRDRVVAGAKKPLGN
EEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GLNLVQAWSQAKQRLEAAGLAGPVIDARLLVEAAADATRADIVTDPYRPLTPEQEARLQ
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHH
DYLARREKREPVSHILGRKGFWKIMLRVTPDVLTPRPDTETVVDVALKAFPEHAPWSVLD
HHHHHHHHCCHHHHHHCCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHCCCCCCCEEEE
LGVGSGAILLAILAERPAAKGLGIDASEEALAVARDNAAALGLAGRTALLRGDWTAGLGD
ECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCEEECCCCEEEEECCCCCCCCC
SAFDLVVSNPPYIASDVLETLEPEVKDYEPRLALEGGADGLDAYRILAPEIVRVLKPGGR
CCEEEEECCCCHHHHHHHHHHCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCC
FAVEIGYDQKDAVEALFRAAGAEDVRTIRDLADRDRVVAGAKKPLGN
EEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]