Click here to switch to the map view.

The map label for this gene is thyA [H]

Identifier: 197104285

GI number: 197104285

Start: 903485

End: 904345

Strand: Reverse

Name: thyA [H]

Synonym: PHZ_c0819

Alternate gene names: 197104285

Gene position: 904345-903485 (Counterclockwise)

Preceding gene: 197104286

Following gene: 197104284

Centisome position: 22.63

GC content: 68.52

Gene sequence:

>861_bases
ATGAACGCCCACGTCGCCCTCGCCCAGCGCTACGCCGCCGCCCCGGACCATCCCGAGCGGCAGTACCTGAACCTGCTGGC
CGACATCCTGGAGACCGGCGCCGCCCGCGGCGACCGCACCGGCACCGGCACCCTGGGCGTCTTCGGCCGCCAGATGCGCT
TTGACCTCTCCCAGGGCTTCCCCCTGCTGACGACCAAGAAGCTGCACCGCAAGTCGATCATCCTGGAACTGCTGTGGTTC
CTGCGCGGCGACACCAACGTGCGCTGGCTGCAGGAGCGCGGGGTGTCCATCTGGGACGAATGGGCCGATGCCGACGGCGA
GCTCGGCCCCGTCTACGGCAAGCAATGGCGCTCTTGGGCGGCCCCGGACGGCCGCGTCATCGACCAGATCTCGGGCGTGG
TGAACAGCCTGAAGACCAATCCCGAGAGCCGCCGCCACATCGTTTCGGCCTGGAATCCGGCCGAGGTGGAGGACATGGCC
CTGCCGCCCTGCCATTGCCTGTTCCAGTTCTTCGTCGCCCCGGACAGGGACGGCGTGAAGCGGCTGTCGTGCCAGCTCTA
CCAGCGTTCGGCCGACGTGTTCCTGGGCGTGCCGTTCAACATCGCCAGCTACGCCCTGCTGACCCTGATGATGGCCAAGG
TGACGGGCTACGCGCCCGGCGAGTTCGTCCACACCCTGGGCGATGCGCACCTCTACCTGAACCACGTCGAGCAGGCCCGA
ATCCAGCTCGCCCGCGAGCCCATGCCGCTGCCGGTCATGCACGTGGCCGACAGGGACGACCTGTTCGCCTTCGCCTACGA
GGACTTCAAGCTCGAGGGCTACCAGGCGCACCCGTCGATCCCGGCCCCGATCGCGGTGTGA

Upstream 100 bases:

>100_bases
GTTGAGAATTCGTAATCTGGGGACGGCGGCCGCCGGGCTGTGGACGACGCGCCCAAGCCGACTGTTCCCGAATCACTTCG
TGCGCGAGGCTCGGCCTCCC

Downstream 100 bases:

>100_bases
TGAGCGGGCGGCTCACCTTCGCCGAGGTCTCGGACGCGGCCGAGAAGATCTCGGACATCTACGCCGGCAAGTACGCCATC
GACCGCGACGACGACTGGTT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MNAHVALAQRYAAAPDHPERQYLNLLADILETGAARGDRTGTGTLGVFGRQMRFDLSQGFPLLTTKKLHRKSIILELLWF
LRGDTNVRWLQERGVSIWDEWADADGELGPVYGKQWRSWAAPDGRVIDQISGVVNSLKTNPESRRHIVSAWNPAEVEDMA
LPPCHCLFQFFVAPDRDGVKRLSCQLYQRSADVFLGVPFNIASYALLTLMMAKVTGYAPGEFVHTLGDAHLYLNHVEQAR
IQLAREPMPLPVMHVADRDDLFAFAYEDFKLEGYQAHPSIPAPIAV

Sequences:

>Translated_286_residues
MNAHVALAQRYAAAPDHPERQYLNLLADILETGAARGDRTGTGTLGVFGRQMRFDLSQGFPLLTTKKLHRKSIILELLWF
LRGDTNVRWLQERGVSIWDEWADADGELGPVYGKQWRSWAAPDGRVIDQISGVVNSLKTNPESRRHIVSAWNPAEVEDMA
LPPCHCLFQFFVAPDRDGVKRLSCQLYQRSADVFLGVPFNIASYALLTLMMAKVTGYAPGEFVHTLGDAHLYLNHVEQAR
IQLAREPMPLPVMHVADRDDLFAFAYEDFKLEGYQAHPSIPAPIAV
>Mature_286_residues
MNAHVALAQRYAAAPDHPERQYLNLLADILETGAARGDRTGTGTLGVFGRQMRFDLSQGFPLLTTKKLHRKSIILELLWF
LRGDTNVRWLQERGVSIWDEWADADGELGPVYGKQWRSWAAPDGRVIDQISGVVNSLKTNPESRRHIVSAWNPAEVEDMA
LPPCHCLFQFFVAPDRDGVKRLSCQLYQRSADVFLGVPFNIASYALLTLMMAKVTGYAPGEFVHTLGDAHLYLNHVEQAR
IQLAREPMPLPVMHVADRDDLFAFAYEDFKLEGYQAHPSIPAPIAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=293, Percent_Identity=49.8293515358362, Blast_Score=287, Evalue=9e-78,
Organism=Escherichia coli, GI1789191, Length=268, Percent_Identity=59.7014925373134, Blast_Score=341, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI71993377, Length=296, Percent_Identity=45.6081081081081, Blast_Score=254, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6324648, Length=293, Percent_Identity=41.6382252559727, Blast_Score=206, Evalue=5e-54,
Organism=Drosophila melanogaster, GI17137556, Length=289, Percent_Identity=50.5190311418685, Blast_Score=281, Evalue=5e-76,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 32123; Mature: 32123

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAHVALAQRYAAAPDHPERQYLNLLADILETGAARGDRTGTGTLGVFGRQMRFDLSQGF
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCC
PLLTTKKLHRKSIILELLWFLRGDTNVRWLQERGVSIWDEWADADGELGPVYGKQWRSWA
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCHHHHHHCCCCCCCCCCCCHHHHCCC
APDGRVIDQISGVVNSLKTNPESRRHIVSAWNPAEVEDMALPPCHCLFQFFVAPDRDGVK
CCCCHHHHHHHHHHHHHHCCCHHHHHEEECCCCCCHHHCCCCHHHHHHHHHHCCCCCHHH
RLSCQLYQRSADVFLGVPFNIASYALLTLMMAKVTGYAPGEFVHTLGDAHLYLNHVEQAR
HHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHH
IQLAREPMPLPVMHVADRDDLFAFAYEDFKLEGYQAHPSIPAPIAV
HHHHCCCCCCCEEEECCCCCEEEEEEECEEECCEECCCCCCCCCCC
>Mature Secondary Structure
MNAHVALAQRYAAAPDHPERQYLNLLADILETGAARGDRTGTGTLGVFGRQMRFDLSQGF
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCC
PLLTTKKLHRKSIILELLWFLRGDTNVRWLQERGVSIWDEWADADGELGPVYGKQWRSWA
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCHHHHHHCCCCCCCCCCCCHHHHCCC
APDGRVIDQISGVVNSLKTNPESRRHIVSAWNPAEVEDMALPPCHCLFQFFVAPDRDGVK
CCCCHHHHHHHHHHHHHHCCCHHHHHEEECCCCCCHHHCCCCHHHHHHHHHHCCCCCHHH
RLSCQLYQRSADVFLGVPFNIASYALLTLMMAKVTGYAPGEFVHTLGDAHLYLNHVEQAR
HHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHH
IQLAREPMPLPVMHVADRDDLFAFAYEDFKLEGYQAHPSIPAPIAV
HHHHCCCCCCCEEEECCCCCEEEEEEECEEECCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]