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The map label for this gene is asnB [H]

Identifier: 197104078

GI number: 197104078

Start: 655979

End: 657742

Strand: Reverse

Name: asnB [H]

Synonym: PHZ_c0612

Alternate gene names: 197104078

Gene position: 657742-655979 (Counterclockwise)

Preceding gene: 197104079

Following gene: 197104077

Centisome position: 16.46

GC content: 74.94

Gene sequence:

>1764_bases
ATGTGCGGTATAGCCGGCCAGCTCGGCGGCGATCCGGCCGCGGCCGGGCCGATGATCCGCGCCCTCGCCCACCGCGGCCC
CGACGGCGTGCGCGAGGAGGCCCTGCCCGGCGCGACCCTGGCCGGCGCGGCCCTGGCCCATGCGCGGCTGTCGATCATCG
ACCTCGAAGGCGGCTGGCAGCCGCTGCACGCCGCGGGCGGGACGGTCGTCGGCAACGGCGAGATCTACAATTACGTCGAA
CTCGCCCGCGACTTCGGCCTTGAGGGGGCGCTCGCCACCGGCTCGGACTTCGAGCCCCTGCTGCATCTCTACGCGCGCGA
AGGCGAGGCCGCCTTCGACCGGCTGCGGGGCATGTACGCCTTCTGCCTGGTGGGCGGCGACGGCCGCGCCTGGCTGGTCC
GCGATCCCTTCGGCATCAAGCCGCTCTACATCATGGAGCACGCCACGGAGAGGGGGACCGGTCTGGCCTTCGCCTCCGAG
CCCCGCGCCTTCCTGCAGGCCGGCCTGATGGCGCCCGGGCTCGACGAGGCCGCCGCCCGCGAGCTGCTGGCCTTCAACTA
CACCGTGGGCGAGCGCACCGCCCTGCAGGGCCTGCGCCGCCTGGCCCCCGGCGAGATCCTCGAGGTTCGCGACGGCCGCA
TCGTCAGCGGACGGCGCCGGCCCTGGCTGGCCTTCCCCGTTCGCGGGGGAGGGGGACCGCCCGCCGGAGAGCGGGTGGCG
GAGGCGGCCCCGCATGGGGAGAAGGCGCTCCTCGATCGGCTCGACGCGGTGCTGGAGGACAGCGTCGCGGTCCATCAGCG
CTCGGACGTCCCCTACGGCCTGTTCCTCTCGGGAGGGATCGATTCGGCGGCCATCGCGACGCTGATGGCCCGGCTCAACG
CGCGGCCGGTCACCGCCTTCACCTGCGGCTTCGACGTTCCTGGCGCGGCGGACGAGCGCGTCCAGGCCGAGGCCGTCGCC
CGGGCGCTGGACCTCGACTGGCGCGAGACCACGTTCGGGGAGGAGGACTTCTGGCGCATCCTGCCGAGGGTCGCCTGGGC
CCTGGACGACCCCACCGCCGACTACGCCGTCCTGCCGACCTACCGGCTCGCCGAGGCCGCCAAGGGGACCCTGACCGTGG
TGCTCACCGGCGAGGGGGGCGACGAGCTGTTCGGCGGCTACGGCCGCTATCGCCGCGCGCTGCGCCCCGCCTGGCTGGGC
GGACGGCCGGCCGAGCCGCGCGGCGCCGATCCCGCCTCGCTGGCCGCCTGGCGCCGGGCGGCCAAGGCGCCGCCGGGCCT
CACGCCCCTGCAGCAGGCCCAGTGGGCAGACATCGCCACCTGGCTGCCGGGCGACCTGCTACTCAAGCTTGACCGCTGCC
TGATGGCCCACGGGTTGGAGGGGCGCACGCCGTTCCTGGACCCAGAGGTCGCCGCCTTCGCCTTCGGCCTGCCCGACCGG
CTCAAGGTGCGCGGCGGCTACGGCAAGTGGATCCTGCGCAAGTGGCTGGAGCGCGCCTGCCCCGCCGCCGCGCCGTGGGC
GCGGAAGCAGGGCTTCACCGTGCCGGTGGACGCCTGGATCGCTCCGCGCGCCCCGGACATCGCCGGCCGGATCGGCCAGG
TTCCCGCCGTGCGGCGCATCCTCGGCGAGGAGGCCGCCGTACGCATGCGGGACGGCGGCCAGGCCCAGCGCTGGCCGCTG
CTGTTTTTCGCCGTCTGGGCCCTGATCCACCTGGACGGCGCGCAACCCGAGGATGCGCTGACGAGTCTCGTCGGCGAGCT
ATGA

Upstream 100 bases:

>100_bases
GCATGACGCGCGCGGGCGAGAAGCGCGTCGCCGCCAGCTTCTCGGAGGCCGCCGTGGTCGCCCGCTGGAAGGCCCTCTTC
GCCGACTTCGGGGAAGGCTG

Downstream 100 bases:

>100_bases
GGAGGGACCGGATGAAGACGCTGATGATGGCCGCGGCCGCGGCCGCCCTGCTGGCCGCGTGCGGCGGCGCCGACGGCCAG
GCCCAGCCGGCGACGAAGGC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MCGIAGQLGGDPAAAGPMIRALAHRGPDGVREEALPGATLAGAALAHARLSIIDLEGGWQPLHAAGGTVVGNGEIYNYVE
LARDFGLEGALATGSDFEPLLHLYAREGEAAFDRLRGMYAFCLVGGDGRAWLVRDPFGIKPLYIMEHATERGTGLAFASE
PRAFLQAGLMAPGLDEAAARELLAFNYTVGERTALQGLRRLAPGEILEVRDGRIVSGRRRPWLAFPVRGGGGPPAGERVA
EAAPHGEKALLDRLDAVLEDSVAVHQRSDVPYGLFLSGGIDSAAIATLMARLNARPVTAFTCGFDVPGAADERVQAEAVA
RALDLDWRETTFGEEDFWRILPRVAWALDDPTADYAVLPTYRLAEAAKGTLTVVLTGEGGDELFGGYGRYRRALRPAWLG
GRPAEPRGADPASLAAWRRAAKAPPGLTPLQQAQWADIATWLPGDLLLKLDRCLMAHGLEGRTPFLDPEVAAFAFGLPDR
LKVRGGYGKWILRKWLERACPAAAPWARKQGFTVPVDAWIAPRAPDIAGRIGQVPAVRRILGEEAAVRMRDGGQAQRWPL
LFFAVWALIHLDGAQPEDALTSLVGEL

Sequences:

>Translated_587_residues
MCGIAGQLGGDPAAAGPMIRALAHRGPDGVREEALPGATLAGAALAHARLSIIDLEGGWQPLHAAGGTVVGNGEIYNYVE
LARDFGLEGALATGSDFEPLLHLYAREGEAAFDRLRGMYAFCLVGGDGRAWLVRDPFGIKPLYIMEHATERGTGLAFASE
PRAFLQAGLMAPGLDEAAARELLAFNYTVGERTALQGLRRLAPGEILEVRDGRIVSGRRRPWLAFPVRGGGGPPAGERVA
EAAPHGEKALLDRLDAVLEDSVAVHQRSDVPYGLFLSGGIDSAAIATLMARLNARPVTAFTCGFDVPGAADERVQAEAVA
RALDLDWRETTFGEEDFWRILPRVAWALDDPTADYAVLPTYRLAEAAKGTLTVVLTGEGGDELFGGYGRYRRALRPAWLG
GRPAEPRGADPASLAAWRRAAKAPPGLTPLQQAQWADIATWLPGDLLLKLDRCLMAHGLEGRTPFLDPEVAAFAFGLPDR
LKVRGGYGKWILRKWLERACPAAAPWARKQGFTVPVDAWIAPRAPDIAGRIGQVPAVRRILGEEAAVRMRDGGQAQRWPL
LFFAVWALIHLDGAQPEDALTSLVGEL
>Mature_587_residues
MCGIAGQLGGDPAAAGPMIRALAHRGPDGVREEALPGATLAGAALAHARLSIIDLEGGWQPLHAAGGTVVGNGEIYNYVE
LARDFGLEGALATGSDFEPLLHLYAREGEAAFDRLRGMYAFCLVGGDGRAWLVRDPFGIKPLYIMEHATERGTGLAFASE
PRAFLQAGLMAPGLDEAAARELLAFNYTVGERTALQGLRRLAPGEILEVRDGRIVSGRRRPWLAFPVRGGGGPPAGERVA
EAAPHGEKALLDRLDAVLEDSVAVHQRSDVPYGLFLSGGIDSAAIATLMARLNARPVTAFTCGFDVPGAADERVQAEAVA
RALDLDWRETTFGEEDFWRILPRVAWALDDPTADYAVLPTYRLAEAAKGTLTVVLTGEGGDELFGGYGRYRRALRPAWLG
GRPAEPRGADPASLAAWRRAAKAPPGLTPLQQAQWADIATWLPGDLLLKLDRCLMAHGLEGRTPFLDPEVAAFAFGLPDR
LKVRGGYGKWILRKWLERACPAAAPWARKQGFTVPVDAWIAPRAPDIAGRIGQVPAVRRILGEEAAVRMRDGGQAQRWPL
LFFAVWALIHLDGAQPEDALTSLVGEL

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=537, Percent_Identity=26.4432029795158, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI168229252, Length=537, Percent_Identity=26.4432029795158, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI168229250, Length=537, Percent_Identity=26.4432029795158, Blast_Score=122, Evalue=8e-28,
Organism=Escherichia coli, GI1786889, Length=542, Percent_Identity=28.0442804428044, Blast_Score=160, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI25147557, Length=288, Percent_Identity=28.8194444444444, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71993933, Length=288, Percent_Identity=28.8194444444444, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321563, Length=567, Percent_Identity=24.8677248677249, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6325403, Length=568, Percent_Identity=25.3521126760563, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45553209, Length=518, Percent_Identity=26.6409266409266, Blast_Score=125, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62954; Mature: 62954

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGQLGGDPAAAGPMIRALAHRGPDGVREEALPGATLAGAALAHARLSIIDLEGGWQ
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHCEEEEEEECCCCC
PLHAAGGTVVGNGEIYNYVELARDFGLEGALATGSDFEPLLHLYAREGEAAFDRLRGMYA
CHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCEEE
FCLVGGDGRAWLVRDPFGIKPLYIMEHATERGTGLAFASEPRAFLQAGLMAPGLDEAAAR
EEEECCCCCEEEEECCCCCCCEEEEECCCCCCCCCEECCCCHHHHHHCCCCCCCCHHHHH
ELLAFNYTVGERTALQGLRRLAPGEILEVRDGRIVSGRRRPWLAFPVRGGGGPPAGERVA
HHHHEECCCCHHHHHHHHHHCCCCCEEEECCCEEECCCCCCEEEEEECCCCCCCCHHHHH
EAAPHGEKALLDRLDAVLEDSVAVHQRSDVPYGLFLSGGIDSAAIATLMARLNARPVTAF
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEE
TCGFDVPGAADERVQAEAVARALDLDWRETTFGEEDFWRILPRVAWALDDPTADYAVLPT
EECCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCEEECCC
YRLAEAAKGTLTVVLTGEGGDELFGGYGRYRRALRPAWLGGRPAEPRGADPASLAAWRRA
HHHHHCCCCEEEEEEECCCCHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
AKAPPGLTPLQQAQWADIATWLPGDLLLKLDRCLMAHGLEGRTPFLDPEVAAFAFGLPDR
HCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCC
LKVRGGYGKWILRKWLERACPAAAPWARKQGFTVPVDAWIAPRAPDIAGRIGQVPAVRRI
EEEECCCHHHHHHHHHHHHCCCCCCCHHHCCCCCEECCCCCCCCCHHHHHHCCCHHHHHH
LGEEAAVRMRDGGQAQRWPLLFFAVWALIHLDGAQPEDALTSLVGEL
HCCHHEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MCGIAGQLGGDPAAAGPMIRALAHRGPDGVREEALPGATLAGAALAHARLSIIDLEGGWQ
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHCEEEEEEECCCCC
PLHAAGGTVVGNGEIYNYVELARDFGLEGALATGSDFEPLLHLYAREGEAAFDRLRGMYA
CHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCEEE
FCLVGGDGRAWLVRDPFGIKPLYIMEHATERGTGLAFASEPRAFLQAGLMAPGLDEAAAR
EEEECCCCCEEEEECCCCCCCEEEEECCCCCCCCCEECCCCHHHHHHCCCCCCCCHHHHH
ELLAFNYTVGERTALQGLRRLAPGEILEVRDGRIVSGRRRPWLAFPVRGGGGPPAGERVA
HHHHEECCCCHHHHHHHHHHCCCCCEEEECCCEEECCCCCCEEEEEECCCCCCCCHHHHH
EAAPHGEKALLDRLDAVLEDSVAVHQRSDVPYGLFLSGGIDSAAIATLMARLNARPVTAF
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEE
TCGFDVPGAADERVQAEAVARALDLDWRETTFGEEDFWRILPRVAWALDDPTADYAVLPT
EECCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCEEECCC
YRLAEAAKGTLTVVLTGEGGDELFGGYGRYRRALRPAWLGGRPAEPRGADPASLAAWRRA
HHHHHCCCCEEEEEEECCCCHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
AKAPPGLTPLQQAQWADIATWLPGDLLLKLDRCLMAHGLEGRTPFLDPEVAAFAFGLPDR
HCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCC
LKVRGGYGKWILRKWLERACPAAAPWARKQGFTVPVDAWIAPRAPDIAGRIGQVPAVRRI
EEEECCCHHHHHHHHHHHHCCCCCCCHHHCCCCCEECCCCCCCCCHHHHHHCCCHHHHHH
LGEEAAVRMRDGGQAQRWPLLFFAVWALIHLDGAQPEDALTSLVGEL
HCCHHEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]