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The map label for this gene is gloB [H]

Identifier: 197103900

GI number: 197103900

Start: 441666

End: 442391

Strand: Reverse

Name: gloB [H]

Synonym: PHZ_c0434

Alternate gene names: 197103900

Gene position: 442391-441666 (Counterclockwise)

Preceding gene: 197103904

Following gene: 197103896

Centisome position: 11.07

GC content: 72.31

Gene sequence:

>726_bases
ATGTCCCTCGCCGTCCACCAGTTTCCCTGCCTGTCCGACAACTACGGCTTCCTCGTGCGCGACGAGGCCAGCGGCCTGGC
CGCCTGCGTCGACACGCCCGAGGCCGGCGCGATCCTGCGCGAGCTGGACCGCCTGGGCTGGACCCTGGCGCTGATCCTCA
ACACCCACTGGCATCCCGACCACGCCGGCGGCAACGAGGAGATCAAGGCCCGCACCGGGGCCAAGGTCGTGGGGCCCAAG
GAGGTCACCCGCATCGCCCCGCTCGACCGCGAGGTCCAGGGGGGCGACGAAGTGCTGCTGGGCGAGACCCGCTTCCGGGT
GATCGAGAGCGGCGGCCACACCCTGGGCCACGTCGCCTACTACGACGCCGAGGACGGGATCGCCTTCGTCGGCGACACCC
TCTTCGCCCTGGGCTGCGGGCGGCTGTTCGAGGGCACGCCGGAACAGATGTGGGCCAGCCTCTCGCGCCTCGCCGCCCTG
CCCGACGACACCCGCGTCTACTGCGCCCACGAATACACCGAGGCGAACGCGCGCTTCGCCCTGTCGCTGGGGGACGATCC
CGTGCTGAAGGCCCGCGCCACCGAGGTCTTCGCCGCCCGCGCCCGAGGCGAGCCCACCGTGCCCACCACCATCGGCCTCG
AGAAGGCCACCAACCCCTTCCTGCGCGCCGCGAACGCGGCCGCCTTCGCCGACGTCCGCGCCGCCAAGGACAGCTTCAAG
GGCTGA

Upstream 100 bases:

>100_bases
GAAGCTCGAGGACGTCCCTGCGCATCTGACTCCCGGCTATCCTCGACGAAGGATCACGACCAGCCTAAACCGGTTCGCAG
CCTCTCAGGAGAGCCTCGCG

Downstream 100 bases:

>100_bases
GCCTCATTAGTCCTCCCCCTCAGGGGGAGGTGGCCCGAAGGGCCAGAGGGGGCTTCCCGCCGCAAGATCGGCCACGAACA
AACACGAACCCACACGAACG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MSLAVHQFPCLSDNYGFLVRDEASGLAACVDTPEAGAILRELDRLGWTLALILNTHWHPDHAGGNEEIKARTGAKVVGPK
EVTRIAPLDREVQGGDEVLLGETRFRVIESGGHTLGHVAYYDAEDGIAFVGDTLFALGCGRLFEGTPEQMWASLSRLAAL
PDDTRVYCAHEYTEANARFALSLGDDPVLKARATEVFAARARGEPTVPTTIGLEKATNPFLRAANAAAFADVRAAKDSFK
G

Sequences:

>Translated_241_residues
MSLAVHQFPCLSDNYGFLVRDEASGLAACVDTPEAGAILRELDRLGWTLALILNTHWHPDHAGGNEEIKARTGAKVVGPK
EVTRIAPLDREVQGGDEVLLGETRFRVIESGGHTLGHVAYYDAEDGIAFVGDTLFALGCGRLFEGTPEQMWASLSRLAAL
PDDTRVYCAHEYTEANARFALSLGDDPVLKARATEVFAARARGEPTVPTTIGLEKATNPFLRAANAAAFADVRAAKDSFK
G
>Mature_240_residues
SLAVHQFPCLSDNYGFLVRDEASGLAACVDTPEAGAILRELDRLGWTLALILNTHWHPDHAGGNEEIKARTGAKVVGPKE
VTRIAPLDREVQGGDEVLLGETRFRVIESGGHTLGHVAYYDAEDGIAFVGDTLFALGCGRLFEGTPEQMWASLSRLAALP
DDTRVYCAHEYTEANARFALSLGDDPVLKARATEVFAARARGEPTVPTTIGLEKATNPFLRAANAAAFADVRAAKDSFKG

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI14150041, Length=232, Percent_Identity=38.3620689655172, Blast_Score=144, Evalue=6e-35,
Organism=Homo sapiens, GI94538322, Length=261, Percent_Identity=35.632183908046, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI94538320, Length=258, Percent_Identity=34.8837209302326, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI116642887, Length=227, Percent_Identity=37.0044052863436, Blast_Score=127, Evalue=9e-30,
Organism=Homo sapiens, GI21703352, Length=226, Percent_Identity=36.7256637168142, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI46361987, Length=184, Percent_Identity=39.1304347826087, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI41327741, Length=181, Percent_Identity=28.7292817679558, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786406, Length=255, Percent_Identity=37.6470588235294, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1787158, Length=176, Percent_Identity=31.8181818181818, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=223, Percent_Identity=35.4260089686099, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17538952, Length=192, Percent_Identity=28.125, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320478, Length=237, Percent_Identity=29.1139240506329, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324614, Length=236, Percent_Identity=26.271186440678, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356335, Length=260, Percent_Identity=37.3076923076923, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24667711, Length=260, Percent_Identity=37.3076923076923, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24667703, Length=258, Percent_Identity=36.8217054263566, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI221330176, Length=166, Percent_Identity=31.9277108433735, Blast_Score=84, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 25894; Mature: 25763

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAVHQFPCLSDNYGFLVRDEASGLAACVDTPEAGAILRELDRLGWTLALILNTHWHPD
CCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHCEEEEEEEECCCCCC
HAGGNEEIKARTGAKVVGPKEVTRIAPLDREVQGGDEVLLGETRFRVIESGGHTLGHVAY
CCCCCCEEECCCCCEEECHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCCEEEEEEE
YDAEDGIAFVGDTLFALGCGRLFEGTPEQMWASLSRLAALPDDTRVYCAHEYTEANARFA
EECCCCEEHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECEECCCCEEE
LSLGDDPVLKARATEVFAARARGEPTVPTTIGLEKATNPFLRAANAAAFADVRAAKDSFK
EECCCCCCCHHHHHHHHHHHCCCCCCCCEEECCHHCCCHHHHHCCHHHHHHHHHHHHHCC
G
C
>Mature Secondary Structure 
SLAVHQFPCLSDNYGFLVRDEASGLAACVDTPEAGAILRELDRLGWTLALILNTHWHPD
CCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHCEEEEEEEECCCCCC
HAGGNEEIKARTGAKVVGPKEVTRIAPLDREVQGGDEVLLGETRFRVIESGGHTLGHVAY
CCCCCCEEECCCCCEEECHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCCEEEEEEE
YDAEDGIAFVGDTLFALGCGRLFEGTPEQMWASLSRLAALPDDTRVYCAHEYTEANARFA
EECCCCEEHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECEECCCCEEE
LSLGDDPVLKARATEVFAARARGEPTVPTTIGLEKATNPFLRAANAAAFADVRAAKDSFK
EECCCCCCCHHHHHHHHHHHCCCCCCCCEEECCHHCCCHHHHHCCHHHHHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA