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The map label for this gene is cysS

Identifier: 197103884

GI number: 197103884

Start: 424374

End: 425786

Strand: Direct

Name: cysS

Synonym: PHZ_c0418

Alternate gene names: 197103884

Gene position: 424374-425786 (Clockwise)

Preceding gene: 197103879

Following gene: 197103885

Centisome position: 10.62

GC content: 70.7

Gene sequence:

>1413_bases
ATGACCCTCAAGCTCTACGACACCATGACCCGCGCCAAGCGGGACTTCGTCCCCTCCGATCCCGAGCGGGTGACGATGTA
TGTCTGTGGGCCGACGGTCTACAACTACGCCCACATCGGCAACTTCCGGCCGGTGGTGGTGTTCGACCTGCTGTTCCGGG
TGCTGCGCGCGCTCTACGGCGAGGACCACGTCCTCTACGCGGCCAACGTCACCGACGTGGACGACAAGATCAACCGCAAG
GCCGCCGAGGAGGGCGTGCCGATCGGGGTGGTCGCCGAGCGCTACCTGGCCGCCTACAACGCCGACGCGCGGGCGCTGGG
CGCGCTGGCCCCCACCTTCCAGCCGAAGGTCACCGAGACGATGGACGCCATCGTCGGCATGATCGGCCGGCTGGTGGACA
ACGGCGCGGCCTACGCCGCCGAGGGCCACGTGCTGTTCTCCACCGAGGCCTTCCCCGACTACGGCAAGCTGTCGGGCCGC
CCGCTCGAGGACCTGATCGCCGGGGCGCGGGTGGATGTGGCGCCCTACAAGAAGGACCCGCGCGACTTCGTGCTGTGGAA
GCCCTCGAAGCCCGGCGAGCCCGAGTGGGAGAGCCCGTGGGGGCCGGGGCGGCCCGGCTGGCACATCGAGTGCTCGGCGA
TGATCGAAGAGGCCCTGGGGCTGCCGATCGACATCCACGGCGGGGGCATCGACCTCGTCTTCCCGCACCACGAGAACGAA
CTGGCCCAGGGGGTGTGCGCGGCGCATCCGCACGGGCATGGCCAGACGTATGCGAGGTACTGGCTGCACAACGGGTTCCT
CAACATGGCCGAGGAGAAGATGAGCAAGAGCGTCGGCAACGTCGCCCTGGCCCACGACCTCCTGAAGACCTGGCCGGGCG
AGGCCCTGCGCTGGGCGCTGCTCTCGGGCCAGTACCGGCAGCCGCTGGAATGGACGGACACGCTGATCGAACAGGCCAAG
AGCGCGCTCGACCGGCTGTACCGGGTGCTGGACGACGCCGCCCGGCCGGGCGATGCAGCGGCCGAGCCCTTCGTCGACCC
GCGCGTAGAGGCGGCGCTGCACGACGACCTCAACACCCCGGCGGCCATGGCGGCGCTGTTCCAGATCTCGGACGAGCTGC
GCTCTGCGATCATGCGCAAGGACGCGGCCGCGGTGGCCGAGGGGCGGGGCCGGCTGGTCGGCTCGGCGAACCTGATGGGC
TTCCTTGCCCAGGCGCCGCAGGCCTGGTTCCAGTCGGGCGCCGACGAGGACCTGAAGGCCCGGGTCGAGGACCTGCTGGC
GCAGCGGCAGGCGGCGCGGGCGGCCAAGGACTGGCCAAGGGCCGACCAGATCCGCGCCGAGCTGGCGGCGCTGAACGTCG
AGGTGATGGACGGACCGTCCGGCGCCACCTGGCGCATCAAGGAGCAGGCCTGA

Upstream 100 bases:

>100_bases
TGAAGGTACGGGTCAGGAGTTCCCGTGGCCTCGCATCCGGGCGAAATGTATATGGGCCTGGCCGCGCCCGCGGCAACCGT
TCCCCAGCGTCAGCGTGACA

Downstream 100 bases:

>100_bases
TGGCGCTGAAGACCCCTGACTACAAGGCGGGACCGTTCGGCCGCGGCGGCGGCATGGGCCCCAAGACCATGAGCCTGAAG
ACCGAGGGCAAGCCGCCCCC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTLKLYDTMTRAKRDFVPSDPERVTMYVCGPTVYNYAHIGNFRPVVVFDLLFRVLRALYGEDHVLYAANVTDVDDKINRK
AAEEGVPIGVVAERYLAAYNADARALGALAPTFQPKVTETMDAIVGMIGRLVDNGAAYAAEGHVLFSTEAFPDYGKLSGR
PLEDLIAGARVDVAPYKKDPRDFVLWKPSKPGEPEWESPWGPGRPGWHIECSAMIEEALGLPIDIHGGGIDLVFPHHENE
LAQGVCAAHPHGHGQTYARYWLHNGFLNMAEEKMSKSVGNVALAHDLLKTWPGEALRWALLSGQYRQPLEWTDTLIEQAK
SALDRLYRVLDDAARPGDAAAEPFVDPRVEAALHDDLNTPAAMAALFQISDELRSAIMRKDAAAVAEGRGRLVGSANLMG
FLAQAPQAWFQSGADEDLKARVEDLLAQRQAARAAKDWPRADQIRAELAALNVEVMDGPSGATWRIKEQA

Sequences:

>Translated_470_residues
MTLKLYDTMTRAKRDFVPSDPERVTMYVCGPTVYNYAHIGNFRPVVVFDLLFRVLRALYGEDHVLYAANVTDVDDKINRK
AAEEGVPIGVVAERYLAAYNADARALGALAPTFQPKVTETMDAIVGMIGRLVDNGAAYAAEGHVLFSTEAFPDYGKLSGR
PLEDLIAGARVDVAPYKKDPRDFVLWKPSKPGEPEWESPWGPGRPGWHIECSAMIEEALGLPIDIHGGGIDLVFPHHENE
LAQGVCAAHPHGHGQTYARYWLHNGFLNMAEEKMSKSVGNVALAHDLLKTWPGEALRWALLSGQYRQPLEWTDTLIEQAK
SALDRLYRVLDDAARPGDAAAEPFVDPRVEAALHDDLNTPAAMAALFQISDELRSAIMRKDAAAVAEGRGRLVGSANLMG
FLAQAPQAWFQSGADEDLKARVEDLLAQRQAARAAKDWPRADQIRAELAALNVEVMDGPSGATWRIKEQA
>Mature_469_residues
TLKLYDTMTRAKRDFVPSDPERVTMYVCGPTVYNYAHIGNFRPVVVFDLLFRVLRALYGEDHVLYAANVTDVDDKINRKA
AEEGVPIGVVAERYLAAYNADARALGALAPTFQPKVTETMDAIVGMIGRLVDNGAAYAAEGHVLFSTEAFPDYGKLSGRP
LEDLIAGARVDVAPYKKDPRDFVLWKPSKPGEPEWESPWGPGRPGWHIECSAMIEEALGLPIDIHGGGIDLVFPHHENEL
AQGVCAAHPHGHGQTYARYWLHNGFLNMAEEKMSKSVGNVALAHDLLKTWPGEALRWALLSGQYRQPLEWTDTLIEQAKS
ALDRLYRVLDDAARPGDAAAEPFVDPRVEAALHDDLNTPAAMAALFQISDELRSAIMRKDAAAVAEGRGRLVGSANLMGF
LAQAPQAWFQSGADEDLKARVEDLLAQRQAARAAKDWPRADQIRAELAALNVEVMDGPSGATWRIKEQA

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=514, Percent_Identity=32.6848249027237, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI10835051, Length=300, Percent_Identity=36.6666666666667, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI62240992, Length=300, Percent_Identity=36.6666666666667, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI21269877, Length=300, Percent_Identity=36.6666666666667, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI303304987, Length=300, Percent_Identity=36.6666666666667, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI1786737, Length=468, Percent_Identity=43.5897435897436, Blast_Score=361, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17509797, Length=251, Percent_Identity=37.0517928286853, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17509795, Length=251, Percent_Identity=37.0517928286853, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324082, Length=320, Percent_Identity=36.875, Blast_Score=198, Evalue=1e-51,
Organism=Drosophila melanogaster, GI19922218, Length=408, Percent_Identity=35.2941176470588, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI19922364, Length=329, Percent_Identity=34.0425531914894, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_PHEZH (B4RE90)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002129261.1
- GeneID:   6789908
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0418
- HOGENOM:   HBG327651
- OMA:   PEEMLAG
- ProtClustDB:   PRK00260
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 51642; Mature: 51511

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 276-276

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKLYDTMTRAKRDFVPSDPERVTMYVCGPTVYNYAHIGNFRPVVVFDLLFRVLRALYG
CEEEHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHC
EDHVLYAANVTDVDDKINRKAAEEGVPIGVVAERYLAAYNADARALGALAPTFQPKVTET
CCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHH
MDAIVGMIGRLVDNGAAYAAEGHVLFSTEAFPDYGKLSGRPLEDLIAGARVDVAPYKKDP
HHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCCCCCCCCHHHHHCCCCEEECCCCCCC
RDFVLWKPSKPGEPEWESPWGPGRPGWHIECSAMIEEALGLPIDIHGGGIDLVFPHHENE
CCEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEECCCCEEEEECCCCCH
LAQGVCAAHPHGHGQTYARYWLHNGFLNMAEEKMSKSVGNVALAHDLLKTWPGEALRWAL
HHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LSGQYRQPLEWTDTLIEQAKSALDRLYRVLDDAARPGDAAAEPFVDPRVEAALHDDLNTP
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCH
AAMAALFQISDELRSAIMRKDAAAVAEGRGRLVGSANLMGFLAQAPQAWFQSGADEDLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCHHHHHHCCCCHHHHH
RVEDLLAQRQAARAAKDWPRADQIRAELAALNVEVMDGPSGATWRIKEQA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEEECCCCCEEEEECCC
>Mature Secondary Structure 
TLKLYDTMTRAKRDFVPSDPERVTMYVCGPTVYNYAHIGNFRPVVVFDLLFRVLRALYG
EEEHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHC
EDHVLYAANVTDVDDKINRKAAEEGVPIGVVAERYLAAYNADARALGALAPTFQPKVTET
CCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHH
MDAIVGMIGRLVDNGAAYAAEGHVLFSTEAFPDYGKLSGRPLEDLIAGARVDVAPYKKDP
HHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCCCCCCCCHHHHHCCCCEEECCCCCCC
RDFVLWKPSKPGEPEWESPWGPGRPGWHIECSAMIEEALGLPIDIHGGGIDLVFPHHENE
CCEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEECCCCEEEEECCCCCH
LAQGVCAAHPHGHGQTYARYWLHNGFLNMAEEKMSKSVGNVALAHDLLKTWPGEALRWAL
HHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LSGQYRQPLEWTDTLIEQAKSALDRLYRVLDDAARPGDAAAEPFVDPRVEAALHDDLNTP
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCH
AAMAALFQISDELRSAIMRKDAAAVAEGRGRLVGSANLMGFLAQAPQAWFQSGADEDLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCHHHHHHCCCCHHHHH
RVEDLLAQRQAARAAKDWPRADQIRAELAALNVEVMDGPSGATWRIKEQA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEEECCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA