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The map label for this gene is proB

Identifier: 197103719

GI number: 197103719

Start: 253376

End: 254503

Strand: Reverse

Name: proB

Synonym: PHZ_c0253

Alternate gene names: 197103719

Gene position: 254503-253376 (Counterclockwise)

Preceding gene: 197103720

Following gene: 197103718

Centisome position: 6.37

GC content: 78.19

Gene sequence:

>1128_bases
GTGAGCGGCCTCGGGCAGGCCCGGCGCGTCGTCGTCAAGGTGGGCTCGGCCCTGCTGGTGGGGCCGGACGGCGCCCCGCA
CGCGGACTGGCTCGCCGACTTCGCCGCCGACGCCGCCCGGATGCGCGCCCGCGGCCAGCAGGTGCTGGTGGTCAGCTCGG
GCGCCGTGGCCCTGGGCCGCCGCCGCCTGGGCCTCGCCCGGCGCGCCCTGACCCTGCCCGAGAAGCAGGCCGCCGCGGCC
GCGGGGCAGTCGGCCCTGATGCGCGCCTGGGAGGAGGCCTTCGAGCCGCACGGCGCGGCCTGCGCCCAGGTGCTGCTGAC
CCGCGACGACACCGAGGTGCGCCGCCGCTGGCTGAACGCCCGGGCCACCGTCGAGACCCTGCTCGGCCTCGGCGTCGTGC
CGGTGATCAACGAGAACGACACCGTCGTCACCGAGGAGATCCGCTACGGCGACAACGACCGCCTGGCCGCCCGCGTGGCC
CAGCTGGTCGGCGCCGACGTCCTCGTGCTGCTGTCCGACGTTGACGGCCTCTACACCGCCGATCCGCGCAAGGACCCGGC
CGCGCGCCACATCCCGCGCGTGCCGCGCCTGACGCCCGAGGTCGAGGCGATGGCCGGCGGGGCGAACGCCGAGGGCGGGG
TCGGCACCGGCGGCATGGCCACCAAGATCGCGGCGGCGCGGATCGCCGCGGCCGGCGGCTGCGCCACCGTGATCACCCTG
GGGAGCCGGACGCGCCCCCTGGCCGCCGTCGAGGGCGGCGAGCGCGCCACGGTCGTCGAGCCCGCCACCACGCCGGCCGC
CGCCTACAAGGCCTGGATCGCCGGCTCCCTGGCCCCGGCCGGCGCGCTGGTGGTGGACGACGGCGCGGCCCAGGCGGTGC
GCCGGGGCAAGAGCCTGCTGGCCGCCGGCGTGCGCCGGGTGGAGGGCCGCTTCGACAAGGGCGATGCGGTGCTCGTCCGC
GACGAGGCCGGCCGCGAGATCGCCCGCGGGCTGGTCCGCTACGAGGCCGCCGACGCCGAGCGGATCTGCGGGCTGAAGAG
CGAGGCGATCGAGGGCGTCCTGGGCTACACCTCCGGCCCCCTGGTCCACGCCGACGACCTGGCGCTGGCGGCCCACGCCG
AGGCCTGA

Upstream 100 bases:

>100_bases
AGAACGTCACCGAGCTGCTGCGCGAGGCCTTCGCCCTGGTGCGCGAGCGCAAGCGCGCGGCGGCCGAAGAGGCGGCGGGG
CCCTCCGAGGGCTGGACGCC

Downstream 100 bases:

>100_bases
GCGCCGCGTCATCTACGACGCCCGGCGGAAGGCCATTTGCTGACCCCGGGAAAGACGCCTAGGAAGTGCTCACCTTGAGC
AAATGGGGCCCATGGTGGAC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSGLGQARRVVVKVGSALLVGPDGAPHADWLADFAADAARMRARGQQVLVVSSGAVALGRRRLGLARRALTLPEKQAAAA
AGQSALMRAWEEAFEPHGAACAQVLLTRDDTEVRRRWLNARATVETLLGLGVVPVINENDTVVTEEIRYGDNDRLAARVA
QLVGADVLVLLSDVDGLYTADPRKDPAARHIPRVPRLTPEVEAMAGGANAEGGVGTGGMATKIAAARIAAAGGCATVITL
GSRTRPLAAVEGGERATVVEPATTPAAAYKAWIAGSLAPAGALVVDDGAAQAVRRGKSLLAAGVRRVEGRFDKGDAVLVR
DEAGREIARGLVRYEAADAERICGLKSEAIEGVLGYTSGPLVHADDLALAAHAEA

Sequences:

>Translated_375_residues
MSGLGQARRVVVKVGSALLVGPDGAPHADWLADFAADAARMRARGQQVLVVSSGAVALGRRRLGLARRALTLPEKQAAAA
AGQSALMRAWEEAFEPHGAACAQVLLTRDDTEVRRRWLNARATVETLLGLGVVPVINENDTVVTEEIRYGDNDRLAARVA
QLVGADVLVLLSDVDGLYTADPRKDPAARHIPRVPRLTPEVEAMAGGANAEGGVGTGGMATKIAAARIAAAGGCATVITL
GSRTRPLAAVEGGERATVVEPATTPAAAYKAWIAGSLAPAGALVVDDGAAQAVRRGKSLLAAGVRRVEGRFDKGDAVLVR
DEAGREIARGLVRYEAADAERICGLKSEAIEGVLGYTSGPLVHADDLALAAHAEA
>Mature_374_residues
SGLGQARRVVVKVGSALLVGPDGAPHADWLADFAADAARMRARGQQVLVVSSGAVALGRRRLGLARRALTLPEKQAAAAA
GQSALMRAWEEAFEPHGAACAQVLLTRDDTEVRRRWLNARATVETLLGLGVVPVINENDTVVTEEIRYGDNDRLAARVAQ
LVGADVLVLLSDVDGLYTADPRKDPAARHIPRVPRLTPEVEAMAGGANAEGGVGTGGMATKIAAARIAAAGGCATVITLG
SRTRPLAAVEGGERATVVEPATTPAAAYKAWIAGSLAPAGALVVDDGAAQAVRRGKSLLAAGVRRVEGRFDKGDAVLVRD
EAGREIARGLVRYEAADAERICGLKSEAIEGVLGYTSGPLVHADDLALAAHAEA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=274, Percent_Identity=32.4817518248175, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI21361368, Length=276, Percent_Identity=32.2463768115942, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1786437, Length=367, Percent_Identity=41.9618528610354, Blast_Score=263, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17569967, Length=254, Percent_Identity=37.7952755905512, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17569969, Length=254, Percent_Identity=37.7952755905512, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320506, Length=421, Percent_Identity=32.541567695962, Blast_Score=192, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6321822, Length=419, Percent_Identity=31.5035799522673, Blast_Score=190, Evalue=4e-49,
Organism=Drosophila melanogaster, GI21357643, Length=300, Percent_Identity=33.6666666666667, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_PHEZH (B4RD63)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002129096.1
- ProteinModelPortal:   B4RD63
- GeneID:   6789743
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0253
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 38734; Mature: 38602

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLGQARRVVVKVGSALLVGPDGAPHADWLADFAADAARMRARGQQVLVVSSGAVALGR
CCCHHHHHHHHHEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHH
RRLGLARRALTLPEKQAAAAAGQSALMRAWEEAFEPHGAACAQVLLTRDDTEVRRRWLNA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
RATVETLLGLGVVPVINENDTVVTEEIRYGDNDRLAARVAQLVGADVLVLLSDVDGLYTA
HHHHHHHHHCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEC
DPRKDPAARHIPRVPRLTPEVEAMAGGANAEGGVGTGGMATKIAAARIAAAGGCATVITL
CCCCCCHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
GSRTRPLAAVEGGERATVVEPATTPAAAYKAWIAGSLAPAGALVVDDGAAQAVRRGKSLL
CCCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHH
AAGVRRVEGRFDKGDAVLVRDEAGREIARGLVRYEAADAERICGLKSEAIEGVLGYTSGP
HHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCC
LVHADDLALAAHAEA
EEECCCCEEEECCCC
>Mature Secondary Structure 
SGLGQARRVVVKVGSALLVGPDGAPHADWLADFAADAARMRARGQQVLVVSSGAVALGR
CCHHHHHHHHHEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHH
RRLGLARRALTLPEKQAAAAAGQSALMRAWEEAFEPHGAACAQVLLTRDDTEVRRRWLNA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
RATVETLLGLGVVPVINENDTVVTEEIRYGDNDRLAARVAQLVGADVLVLLSDVDGLYTA
HHHHHHHHHCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEC
DPRKDPAARHIPRVPRLTPEVEAMAGGANAEGGVGTGGMATKIAAARIAAAGGCATVITL
CCCCCCHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
GSRTRPLAAVEGGERATVVEPATTPAAAYKAWIAGSLAPAGALVVDDGAAQAVRRGKSLL
CCCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHH
AAGVRRVEGRFDKGDAVLVRDEAGREIARGLVRYEAADAERICGLKSEAIEGVLGYTSGP
HHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCC
LVHADDLALAAHAEA
EEECCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA