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The map label for this gene is pbpF [H]

Identifier: 197103697

GI number: 197103697

Start: 232587

End: 234629

Strand: Reverse

Name: pbpF [H]

Synonym: PHZ_c0231

Alternate gene names: 197103697

Gene position: 234629-232587 (Counterclockwise)

Preceding gene: 197103699

Following gene: 197103693

Centisome position: 5.87

GC content: 71.66

Gene sequence:

>2043_bases
ATGGCTCGCCCGCCGGGGCTGGGGCGGCCGCAATCGGTTAGGACGACGCAAGAAATGGCCAATGCCGGAGGGGGCGGTCG
GGCGCCCCGGCCGCCGCGCGGAAAACGCTCGCCCCTGCAGGCGCTGATCTACTGGACCCTGGTGCTGGGCGTCTGGGGGC
TGATCTTCCTGGTGGCGTTCTTCGCCGTCTTCGCCGTCGACCTGCCCGACACCTCGACCCTCTACGACGTCAAGCGCCAG
CCGTCGGTGTCGTACCTCGACCGCTCGGGCGCGCTCGTCGCCGTGCGCGGCAGCCAGTACGCCCCGCCGGTGGACCTCGA
CCGCCTGCCCGAGCACGTGCCCGAGGCGTTCATCGCCATCGAGGACCGCTGGTACTATTGGCACTTCGGCTTCAACCCCT
GGGGCATCGTCCGCAGCCAGCTCTACAACATGCGCCGCGAGAGCGGCCCCCTGCGCGGCGGCTCGACCATCACCCAGCAG
CTCGCCCGCAACCTCTTCCTGACGCCGTCGCAGAACTACCGGCGCAAGGCCCAGGAGCTGATCCTGGCGGTCTGGCTGGA
GGCCCGCTTCTCGAAGAAGCAGATCCTCGAGCTCTACCTGAACCGGGTCTACTTCGGCGCCGGCGCCTACGGCATCGAGG
CCGCCTCCCAGCGCTACTTCGGCAAGCCGGCCGAGAAGCTGACCCTGGGCGAATCCGCCCTGCTGGCCGGGATGATGAAG
GGCCCCTCGCGCTACTCGCCGGTCTCGGCCACCGAGCGCGCCGCCCGCCGCGCCACCGTCGTGCTCGACAAGATGGTCGA
GGCGGACTTCATCACGCCCCAGGAACGCGAGGCCGCCATCCGCACCAAGGTGCGGGTCAATCCCGTGCTGGCCAACCAGC
GGGCGCAGTACTTCACCGACTGGGTGGACGACCAGGTCCGCAGCCTGGTGGGCGAGCCCACCGAGGACCTCGTCGTGGAG
ACCACCCTCGACCTGCCGCTGCAGGCCGCCGCCGAGCAGGCGCTCAAGCGGGGCGTCGAGGCGGCCAAGGACCAGGGCGT
CGAGCAGGGCGCCCTCGTCTCGATCGACGGCGAGGGCCGCGTGCGCGCCTACGTCGGCGGGACCAACTACCTGCAGACCC
AGTTCGACCGCGCCACCATGGCCCGCCGCCAGGCCGGCTCGGCGTTCAAGCCGTTCGTCTACCTGACCGCCGTCGAGGCG
GGCCGCACGCCGAACACCCCGGTCGTCGACGAGCCGATCAAGATCGGCAACTGGGAGCCGCGCAACTACACCCAGCGCTA
CCTCGGCCCGATGACGCTGCAGACCGCCCTGCAGGAGTCCATCAACACCGTCGCCGCGCGCCTGGCCAACGAGGTCGGCA
CCTCCAACGTCGCGGCCACCGCCCGCCGGCTCGGCATCACCTCGAACATCCAGCTCGACCCCTCGATGGCGCTGGGCGCG
GTCGAGGTCAGCCCGCTGGAGATGGCCCAGGCCTTCGCCCCCTTCGCCAACGGCGGCTTCCTGGCCCGCGGCTACGGGAT
CGAGCGCATCCGCACCTCCAGCGGCCGGGTGCTCTACGACCACAGCGTCGACCAGGCGCCGCGCCAGGCGGTGATCGGCC
AGCCGGCGCTCGCCTACATGAACCAGATGATGCGCCAGGTGATCCTGCAGGGCACGGGCGCCAGGGCCAACGTCCCCGGC
TACGACATCGCCGGCAAGACCGGCACCACCAGCGACTACCGCGACGCCTGGTTCGTCGGCTACACGGGCGGCTTCGTCAC
CGCGGTCTGGGTCGGCAAGGACAACAACACCCCGATGCGCCGGGTGACCGGCGGCGGCGCGCCCGCGGGCATCTGGAAGG
ACTTCATGACCGCGGCCCTGCCGCGCCTGGCGGTCCAGCCGATCCCCGGCAGCGACCTGCCGCCGCCGCCCGAGCCGGAC
CCCATCGGCGACCTGATCGACGGGGTCACCGACTTCTTCGGCGGCCGCCGCGACGAGACCCCGCCGTCCGAGTTCGACGA
GCCGGCCCCGCCCCGCCGCGCCCGCGAGGAACCGCTGTTCTAG

Upstream 100 bases:

>100_bases
TGCGCAACGCCGCTATGCGGGAATGCAGAGCTGGGCCGCTGCGGGCGCCTGCGGCGTTCATGTCCCATAAACCTTGTTGG
GCGTTCCGTGCCGCTGGGTT

Downstream 100 bases:

>100_bases
GGAGCCGCTGTTCTAGGACGCGTCCGTCACCGCCCGAAGGCGTGGAGCCGGGCGCCTTCGGCGCGGAGCCACTCGCGGTG
GCGGCGCTCGTCGCCCACCA

Product: membrane carboxypeptidase (penicillin-binding protein)

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 680; Mature: 679

Protein sequence:

>680_residues
MARPPGLGRPQSVRTTQEMANAGGGGRAPRPPRGKRSPLQALIYWTLVLGVWGLIFLVAFFAVFAVDLPDTSTLYDVKRQ
PSVSYLDRSGALVAVRGSQYAPPVDLDRLPEHVPEAFIAIEDRWYYWHFGFNPWGIVRSQLYNMRRESGPLRGGSTITQQ
LARNLFLTPSQNYRRKAQELILAVWLEARFSKKQILELYLNRVYFGAGAYGIEAASQRYFGKPAEKLTLGESALLAGMMK
GPSRYSPVSATERAARRATVVLDKMVEADFITPQEREAAIRTKVRVNPVLANQRAQYFTDWVDDQVRSLVGEPTEDLVVE
TTLDLPLQAAAEQALKRGVEAAKDQGVEQGALVSIDGEGRVRAYVGGTNYLQTQFDRATMARRQAGSAFKPFVYLTAVEA
GRTPNTPVVDEPIKIGNWEPRNYTQRYLGPMTLQTALQESINTVAARLANEVGTSNVAATARRLGITSNIQLDPSMALGA
VEVSPLEMAQAFAPFANGGFLARGYGIERIRTSSGRVLYDHSVDQAPRQAVIGQPALAYMNQMMRQVILQGTGARANVPG
YDIAGKTGTTSDYRDAWFVGYTGGFVTAVWVGKDNNTPMRRVTGGGAPAGIWKDFMTAALPRLAVQPIPGSDLPPPPEPD
PIGDLIDGVTDFFGGRRDETPPSEFDEPAPPRRAREEPLF

Sequences:

>Translated_680_residues
MARPPGLGRPQSVRTTQEMANAGGGGRAPRPPRGKRSPLQALIYWTLVLGVWGLIFLVAFFAVFAVDLPDTSTLYDVKRQ
PSVSYLDRSGALVAVRGSQYAPPVDLDRLPEHVPEAFIAIEDRWYYWHFGFNPWGIVRSQLYNMRRESGPLRGGSTITQQ
LARNLFLTPSQNYRRKAQELILAVWLEARFSKKQILELYLNRVYFGAGAYGIEAASQRYFGKPAEKLTLGESALLAGMMK
GPSRYSPVSATERAARRATVVLDKMVEADFITPQEREAAIRTKVRVNPVLANQRAQYFTDWVDDQVRSLVGEPTEDLVVE
TTLDLPLQAAAEQALKRGVEAAKDQGVEQGALVSIDGEGRVRAYVGGTNYLQTQFDRATMARRQAGSAFKPFVYLTAVEA
GRTPNTPVVDEPIKIGNWEPRNYTQRYLGPMTLQTALQESINTVAARLANEVGTSNVAATARRLGITSNIQLDPSMALGA
VEVSPLEMAQAFAPFANGGFLARGYGIERIRTSSGRVLYDHSVDQAPRQAVIGQPALAYMNQMMRQVILQGTGARANVPG
YDIAGKTGTTSDYRDAWFVGYTGGFVTAVWVGKDNNTPMRRVTGGGAPAGIWKDFMTAALPRLAVQPIPGSDLPPPPEPD
PIGDLIDGVTDFFGGRRDETPPSEFDEPAPPRRAREEPLF
>Mature_679_residues
ARPPGLGRPQSVRTTQEMANAGGGGRAPRPPRGKRSPLQALIYWTLVLGVWGLIFLVAFFAVFAVDLPDTSTLYDVKRQP
SVSYLDRSGALVAVRGSQYAPPVDLDRLPEHVPEAFIAIEDRWYYWHFGFNPWGIVRSQLYNMRRESGPLRGGSTITQQL
ARNLFLTPSQNYRRKAQELILAVWLEARFSKKQILELYLNRVYFGAGAYGIEAASQRYFGKPAEKLTLGESALLAGMMKG
PSRYSPVSATERAARRATVVLDKMVEADFITPQEREAAIRTKVRVNPVLANQRAQYFTDWVDDQVRSLVGEPTEDLVVET
TLDLPLQAAAEQALKRGVEAAKDQGVEQGALVSIDGEGRVRAYVGGTNYLQTQFDRATMARRQAGSAFKPFVYLTAVEAG
RTPNTPVVDEPIKIGNWEPRNYTQRYLGPMTLQTALQESINTVAARLANEVGTSNVAATARRLGITSNIQLDPSMALGAV
EVSPLEMAQAFAPFANGGFLARGYGIERIRTSSGRVLYDHSVDQAPRQAVIGQPALAYMNQMMRQVILQGTGARANVPGY
DIAGKTGTTSDYRDAWFVGYTGGFVTAVWVGKDNNTPMRRVTGGGAPAGIWKDFMTAALPRLAVQPIPGSDLPPPPEPDP
IGDLIDGVTDFFGGRRDETPPSEFDEPAPPRRAREEPLF

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=576, Percent_Identity=35.5902777777778, Blast_Score=254, Evalue=9e-69,
Organism=Escherichia coli, GI87082258, Length=307, Percent_Identity=36.4820846905537, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788867, Length=506, Percent_Identity=29.0513833992095, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1789601, Length=168, Percent_Identity=39.8809523809524, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 74613; Mature: 74481

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPPGLGRPQSVRTTQEMANAGGGGRAPRPPRGKRSPLQALIYWTLVLGVWGLIFLVAF
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FAVFAVDLPDTSTLYDVKRQPSVSYLDRSGALVAVRGSQYAPPVDLDRLPEHVPEAFIAI
HHHHCCCCCCCCHHHHHCCCCCHHHHCCCCCEEEECCCCCCCCCCHHHHHHHCCCEEEEE
EDRWYYWHFGFNPWGIVRSQLYNMRRESGPLRGGSTITQQLARNLFLTPSQNYRRKAQEL
ECCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCHHHHHHHHHH
ILAVWLEARFSKKQILELYLNRVYFGAGAYGIEAASQRYFGKPAEKLTLGESALLAGMMK
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHC
GPSRYSPVSATERAARRATVVLDKMVEADFITPQEREAAIRTKVRVNPVLANQRAQYFTD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECHHHHCCHHHHHHH
WVDDQVRSLVGEPTEDLVVETTLDLPLQAAAEQALKRGVEAAKDQGVEQGALVSIDGEGR
HHHHHHHHHHCCCCHHEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCE
VRAYVGGTNYLQTQFDRATMARRQAGSAFKPFVYLTAVEAGRTPNTPVVDEPIKIGNWEP
EEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCC
RNYTQRYLGPMTLQTALQESINTVAARLANEVGTSNVAATARRLGITSNIQLDPSMALGA
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEECCCCCCCE
VEVSPLEMAQAFAPFANGGFLARGYGIERIRTSSGRVLYDHSVDQAPRQAVIGQPALAYM
EECCHHHHHHHHCCCCCCCEEEECCCHHHEECCCCCEEEECCCCCCHHHHHCCCHHHHHH
NQMMRQVILQGTGARANVPGYDIAGKTGTTSDYRDAWFVGYTGGFVTAVWVGKDNNTPMR
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCHH
RVTGGGAPAGIWKDFMTAALPRLAVQPIPGSDLPPPPEPDPIGDLIDGVTDFFGGRRDET
EECCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
PPSEFDEPAPPRRAREEPLF
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARPPGLGRPQSVRTTQEMANAGGGGRAPRPPRGKRSPLQALIYWTLVLGVWGLIFLVAF
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FAVFAVDLPDTSTLYDVKRQPSVSYLDRSGALVAVRGSQYAPPVDLDRLPEHVPEAFIAI
HHHHCCCCCCCCHHHHHCCCCCHHHHCCCCCEEEECCCCCCCCCCHHHHHHHCCCEEEEE
EDRWYYWHFGFNPWGIVRSQLYNMRRESGPLRGGSTITQQLARNLFLTPSQNYRRKAQEL
ECCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCHHHHHHHHHH
ILAVWLEARFSKKQILELYLNRVYFGAGAYGIEAASQRYFGKPAEKLTLGESALLAGMMK
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHC
GPSRYSPVSATERAARRATVVLDKMVEADFITPQEREAAIRTKVRVNPVLANQRAQYFTD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECHHHHCCHHHHHHH
WVDDQVRSLVGEPTEDLVVETTLDLPLQAAAEQALKRGVEAAKDQGVEQGALVSIDGEGR
HHHHHHHHHHCCCCHHEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCE
VRAYVGGTNYLQTQFDRATMARRQAGSAFKPFVYLTAVEAGRTPNTPVVDEPIKIGNWEP
EEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCC
RNYTQRYLGPMTLQTALQESINTVAARLANEVGTSNVAATARRLGITSNIQLDPSMALGA
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEECCCCCCCE
VEVSPLEMAQAFAPFANGGFLARGYGIERIRTSSGRVLYDHSVDQAPRQAVIGQPALAYM
EECCHHHHHHHHCCCCCCCEEEECCCHHHEECCCCCEEEECCCCCCHHHHHCCCHHHHHH
NQMMRQVILQGTGARANVPGYDIAGKTGTTSDYRDAWFVGYTGGFVTAVWVGKDNNTPMR
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCHH
RVTGGGAPAGIWKDFMTAALPRLAVQPIPGSDLPPPPEPDPIGDLIDGVTDFFGGRRDET
EECCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
PPSEFDEPAPPRRAREEPLF
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]