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The map label for this gene is ptsN [H]

Identifier: 197103647

GI number: 197103647

Start: 176131

End: 176598

Strand: Direct

Name: ptsN [H]

Synonym: PHZ_c0181

Alternate gene names: 197103647

Gene position: 176131-176598 (Clockwise)

Preceding gene: 197103646

Following gene: 197103654

Centisome position: 4.41

GC content: 75.85

Gene sequence:

>468_bases
ATGCACATCGGCGACCTTCTCGATCGCACCGCGATCTCCCTGCGCGTCAGCGCGTCCAGCAAGCGTCAGGTCCTGGCCGT
GGTCGCCGAGATCGCCGCCCGCAAGCTCGGCCTCGAGGCGGGGGCGGTGCTGGACGCCCTGCTCGAGCGGGAGGCCGCCG
GCTCCACCGGGGTGGGCTACGGCGTCGCCGCGCCCCACGCCCGGCTGGAGGGCCTGGACCGCATGCGCGGCGTCTTCGTG
CGCCTGGAGCAGCCGGTGGACTTCGACGCCGTGGACGACCAGCCCGTGGACCTGGTGTTCGCCCTGTTCGCCCCCAAGGA
CGCGGGCGCCGACCACCTGCGGGCCCTGGCCAGGGTCTCGCGCCTGCTGCGCCAGGCAGAGGCGCGCGAGCAGCTGCGCC
AGGCGCGGACGGCCGACGCCGTCTACGCGCTGCTGGTGCAGGACGCCGCCCCCCGCTCGGCGGCCTGA

Upstream 100 bases:

>100_bases
CGGGGAGGGAACCGGTCCGGCCCACTAGCGCTAACGAACGATCGTCACACGCCCCCGCTAGAGGCGCGGCCGACGGTCAG
TAGTGAGTTCAAGACGAAGC

Downstream 100 bases:

>100_bases
AACCCCGATCCTGAAGCCGCAACGAAAAAGGCCGGCGTCTCCGCCGGCCTTTCCCGCATTTCGCGGTGAACTCTTCGCTT
AACGGTCGGGCCTAGTGGAC

Product: PTS system, nitrogen regulatory IIA component ptsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MHIGDLLDRTAISLRVSASSKRQVLAVVAEIAARKLGLEAGAVLDALLEREAAGSTGVGYGVAAPHARLEGLDRMRGVFV
RLEQPVDFDAVDDQPVDLVFALFAPKDAGADHLRALARVSRLLRQAEAREQLRQARTADAVYALLVQDAAPRSAA

Sequences:

>Translated_155_residues
MHIGDLLDRTAISLRVSASSKRQVLAVVAEIAARKLGLEAGAVLDALLEREAAGSTGVGYGVAAPHARLEGLDRMRGVFV
RLEQPVDFDAVDDQPVDLVFALFAPKDAGADHLRALARVSRLLRQAEAREQLRQARTADAVYALLVQDAAPRSAA
>Mature_155_residues
MHIGDLLDRTAISLRVSASSKRQVLAVVAEIAARKLGLEAGAVLDALLEREAAGSTGVGYGVAAPHARLEGLDRMRGVFV
RLEQPVDFDAVDDQPVDLVFALFAPKDAGADHLRALARVSRLLRQAEAREQLRQARTADAVYALLVQDAAPRSAA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=148, Percent_Identity=40.5405405405405, Blast_Score=121, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16547; Mature: 16547

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIGDLLDRTAISLRVSASSKRQVLAVVAEIAARKLGLEAGAVLDALLEREAAGSTGVGY
CCHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
GVAAPHARLEGLDRMRGVFVRLEQPVDFDAVDDQPVDLVFALFAPKDAGADHLRALARVS
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHEECCCCCCCHHHHHHHHHHH
RLLRQAEAREQLRQARTADAVYALLVQDAAPRSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHIGDLLDRTAISLRVSASSKRQVLAVVAEIAARKLGLEAGAVLDALLEREAAGSTGVGY
CCHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
GVAAPHARLEGLDRMRGVFVRLEQPVDFDAVDDQPVDLVFALFAPKDAGADHLRALARVS
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHEECCCCCCCHHHHHHHHHHH
RLLRQAEAREQLRQARTADAVYALLVQDAAPRSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]