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The map label for this gene is ligT [C]

Identifier: 197103613

GI number: 197103613

Start: 137847

End: 138383

Strand: Direct

Name: ligT [C]

Synonym: PHZ_c0147

Alternate gene names: 197103613

Gene position: 137847-138383 (Clockwise)

Preceding gene: 197103612

Following gene: 197103616

Centisome position: 3.45

GC content: 73.18

Gene sequence:

>537_bases
ATGATCCGCCTGTTCGCCGCCCTGCCGCTGCCGCCCGACATCGTCCGCGGCCTCGTCAGACGCCAGACGGGCGTCGACGG
CGCGCGCTGGCGCGAACCCGACAGCCTGCACATCACCCTGCGCTTCTTCGGCGAGGTGCGCGAGGACGTGGCCCGCGACC
TCGACACCGAGCTCGCCGGCATCGACGCCCCGCCATTCGAGATCGAGCTGGAGGGCGCCGGCGCGTTCGGCGAGGGCCGC
GACATCCATGCGATCTGGGCCGGGGTCGCCGAGAGCGCGCCGCTGCGCCGGCTGGCCGAGGCCTGCGAGACCGCCGCCCG
CCGGGCGGGCCTCAAGCCCGAGAAGCGCCGCTACCGGCCGCACGTCACCCTGGCCTATCTCAAGCAAGCCGACCCGCCGG
AAGTCGCCGCCTGGATCCAGGCCAACAACCTCCTGAAGTCGCCGCCGATCCCGGTGGACCGCTTCGCCCTCTACTCCAGC
GTCCTGGGCTCCGAACGCGCCCTCTACCGCGTCGAGGCCGAGTACCCGCTAGGGTGA

Upstream 100 bases:

>100_bases
CGAACGCCGCCGGCGTCGAGGTCATCGCCCGGCGGCTCGCCCCGTCCGTGGCGAAAGCCCTGATGACCGGACGCACGGCC
GCGGGCTAAGAGGCGAGGCC

Downstream 100 bases:

>100_bases
CCTAGCAGGCCGATCCCACCTTCAGCCGGGTCTTGGTCTGGGCCAGGGTCTCGCCGTGACCGACGGCTTGGCGCAGCGCA
TCGACGCGGGCGAGGTCCTC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MIRLFAALPLPPDIVRGLVRRQTGVDGARWREPDSLHITLRFFGEVREDVARDLDTELAGIDAPPFEIELEGAGAFGEGR
DIHAIWAGVAESAPLRRLAEACETAARRAGLKPEKRRYRPHVTLAYLKQADPPEVAAWIQANNLLKSPPIPVDRFALYSS
VLGSERALYRVEAEYPLG

Sequences:

>Translated_178_residues
MIRLFAALPLPPDIVRGLVRRQTGVDGARWREPDSLHITLRFFGEVREDVARDLDTELAGIDAPPFEIELEGAGAFGEGR
DIHAIWAGVAESAPLRRLAEACETAARRAGLKPEKRRYRPHVTLAYLKQADPPEVAAWIQANNLLKSPPIPVDRFALYSS
VLGSERALYRVEAEYPLG
>Mature_178_residues
MIRLFAALPLPPDIVRGLVRRQTGVDGARWREPDSLHITLRFFGEVREDVARDLDTELAGIDAPPFEIELEGAGAFGEGR
DIHAIWAGVAESAPLRRLAEACETAARRAGLKPEKRRYRPHVTLAYLKQADPPEVAAWIQANNLLKSPPIPVDRFALYSS
VLGSERALYRVEAEYPLG

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 19763; Mature: 19763

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLFAALPLPPDIVRGLVRRQTGVDGARWREPDSLHITLRFFGEVREDVARDLDTELAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHC
IDAPPFEIELEGAGAFGEGRDIHAIWAGVAESAPLRRLAEACETAARRAGLKPEKRRYRP
CCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHCCC
HVTLAYLKQADPPEVAAWIQANNLLKSPPIPVDRFALYSSVLGSERALYRVEAEYPLG
CEEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MIRLFAALPLPPDIVRGLVRRQTGVDGARWREPDSLHITLRFFGEVREDVARDLDTELAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHC
IDAPPFEIELEGAGAFGEGRDIHAIWAGVAESAPLRRLAEACETAARRAGLKPEKRRYRP
CCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHCCC
HVTLAYLKQADPPEVAAWIQANNLLKSPPIPVDRFALYSSVLGSERALYRVEAEYPLG
CEEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]