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The map label for this gene is dapF

Identifier: 197103593

GI number: 197103593

Start: 120599

End: 121432

Strand: Direct

Name: dapF

Synonym: PHZ_c0127

Alternate gene names: 197103593

Gene position: 120599-121432 (Clockwise)

Preceding gene: 197103592

Following gene: 197103594

Centisome position: 3.02

GC content: 74.1

Gene sequence:

>834_bases
ATGTCCGCGGCCATGGGACGTCCGTTCCTCAAGATGAACGGGCTCGGCAACGACTTCGTCGTGGTCGAGACCCGCTCCGC
GCCGTTCGAACCGACGGCGGCGCAGGTGCGCGCGATCGCCGACCGCGCGACCGGGATCGGCTGCGACCAGCTGATCGCCG
TCGACCCTGCCGAGGGCGCCGACGCCCACGTCCGCTTCTGGAACGCCGACGGCGAGGAGGTGGGGGCCTGCGGCAACGGC
ACGCGCTGCGTCGGCTGGCTGCTCATGCAGTCCAGCGGCAAGGACGAGGCGGTGATCGAGACGCGGGCGGGGCGCCTCGT
CGCCACCCGCGCGGGCGAGCGGCTGGTCAGCGTCGACATGGGCCCGCCGCGGCTGGAGTGGAACGAGATCCCGCTCGCCG
AGCCGCACGACACCCGCGCGCTGGACGTCGTCCTATACCACCACGCCGACCTGATGGCGCCGCCCGGCTGCGTCTCGATG
GGCAACCCGCACGTGGTCTTCTTCGTGCCCGACGCCGAGCAGGCGCCGGCGGCCGAGGCCGGCCCCGCGATCGAAGGGCA
CCCGCTGTTCCCCGAGCACGTCAACGTCGGCTTCGCCCAGGTGAAGGCGCCGGACCGCATCCGCCTGCGGGTGTGGGAGC
GCGGCGCGGGGCTGACGAAGGCCTGCGGCACCGGCGCCTGCGCGGCCATGGTGGCCGCCTCGCGCCGCGGCCTGATCGGC
CGCCACGCGACGATGGAGCTGGACGGCGGCGAGCTGTTCGTCGAATGGCGCGCGGACGGCCACGTGATCATGACCGGCCC
GGCGGCCGTCGACTTCCAGGGCGAGCTGCCGTGA

Upstream 100 bases:

>100_bases
GCCCTGAGCGGCCGCCGCTCGGGTCCGGTGACGGGGGCCACCGCGGCCGCCTGACCAGGCGCCGAAGCGGCGAGGTGCAG
CGCGCGCCGTTCTCGCCTAT

Downstream 100 bases:

>100_bases
GCGGCTTCGAGGCCCCCTCCGTCTCGACGCGTTCGCGCCGATCCACCTCCCCCCCTTCGCAGGGGAGGAGCGACGTCGAC
ATCGTCACGTTCGGCTGCCG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSAAMGRPFLKMNGLGNDFVVVETRSAPFEPTAAQVRAIADRATGIGCDQLIAVDPAEGADAHVRFWNADGEEVGACGNG
TRCVGWLLMQSSGKDEAVIETRAGRLVATRAGERLVSVDMGPPRLEWNEIPLAEPHDTRALDVVLYHHADLMAPPGCVSM
GNPHVVFFVPDAEQAPAAEAGPAIEGHPLFPEHVNVGFAQVKAPDRIRLRVWERGAGLTKACGTGACAAMVAASRRGLIG
RHATMELDGGELFVEWRADGHVIMTGPAAVDFQGELP

Sequences:

>Translated_277_residues
MSAAMGRPFLKMNGLGNDFVVVETRSAPFEPTAAQVRAIADRATGIGCDQLIAVDPAEGADAHVRFWNADGEEVGACGNG
TRCVGWLLMQSSGKDEAVIETRAGRLVATRAGERLVSVDMGPPRLEWNEIPLAEPHDTRALDVVLYHHADLMAPPGCVSM
GNPHVVFFVPDAEQAPAAEAGPAIEGHPLFPEHVNVGFAQVKAPDRIRLRVWERGAGLTKACGTGACAAMVAASRRGLIG
RHATMELDGGELFVEWRADGHVIMTGPAAVDFQGELP
>Mature_276_residues
SAAMGRPFLKMNGLGNDFVVVETRSAPFEPTAAQVRAIADRATGIGCDQLIAVDPAEGADAHVRFWNADGEEVGACGNGT
RCVGWLLMQSSGKDEAVIETRAGRLVATRAGERLVSVDMGPPRLEWNEIPLAEPHDTRALDVVLYHHADLMAPPGCVSMG
NPHVVFFVPDAEQAPAAEAGPAIEGHPLFPEHVNVGFAQVKAPDRIRLRVWERGAGLTKACGTGACAAMVAASRRGLIGR
HATMELDGGELFVEWRADGHVIMTGPAAVDFQGELP

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=273, Percent_Identity=42.4908424908425, Blast_Score=200, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_PHEZH (B4RCE2)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002128970.1
- ProteinModelPortal:   B4RCE2
- GeneID:   6789615
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0127
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 29373; Mature: 29242

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 77-77 ACT_SITE 222-222

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAMGRPFLKMNGLGNDFVVVETRSAPFEPTAAQVRAIADRATGIGCDQLIAVDPAEGA
CCCCCCCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCC
DAHVRFWNADGEEVGACGNGTRCVGWLLMQSSGKDEAVIETRAGRLVATRAGERLVSVDM
CCEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCEEEEECCCCEEEEECC
GPPRLEWNEIPLAEPHDTRALDVVLYHHADLMAPPGCVSMGNPHVVFFVPDAEQAPAAEA
CCCCCCCCCCCCCCCCCCCEEEEEEEECHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCC
GPAIEGHPLFPEHVNVGFAQVKAPDRIRLRVWERGAGLTKACGTGACAAMVAASRRGLIG
CCCCCCCCCCHHHCCCCEEEECCCCEEEEEEEECCCCCCHHCCCHHHHHHHHHHHCCCEE
RHATMELDGGELFVEWRADGHVIMTGPAAVDFQGELP
CCEEEEECCCEEEEEEECCCEEEEECCEEEEECCCCC
>Mature Secondary Structure 
SAAMGRPFLKMNGLGNDFVVVETRSAPFEPTAAQVRAIADRATGIGCDQLIAVDPAEGA
CCCCCCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCC
DAHVRFWNADGEEVGACGNGTRCVGWLLMQSSGKDEAVIETRAGRLVATRAGERLVSVDM
CCEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCEEEEECCCCEEEEECC
GPPRLEWNEIPLAEPHDTRALDVVLYHHADLMAPPGCVSMGNPHVVFFVPDAEQAPAAEA
CCCCCCCCCCCCCCCCCCCEEEEEEEECHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCC
GPAIEGHPLFPEHVNVGFAQVKAPDRIRLRVWERGAGLTKACGTGACAAMVAASRRGLIG
CCCCCCCCCCHHHCCCCEEEECCCCEEEEEEEECCCCCCHHCCCHHHHHHHHHHHCCCEE
RHATMELDGGELFVEWRADGHVIMTGPAAVDFQGELP
CCEEEEECCCEEEEEEECCCEEEEECCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA