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The map label for this gene is leuC

Identifier: 197103573

GI number: 197103573

Start: 98616

End: 100040

Strand: Reverse

Name: leuC

Synonym: PHZ_c0107

Alternate gene names: 197103573

Gene position: 100040-98616 (Counterclockwise)

Preceding gene: 197103576

Following gene: 197103572

Centisome position: 2.5

GC content: 72.35

Gene sequence:

>1425_bases
ATGCCCGGCAAGACCCTCTACGACAAGATCTGGGACGCCCATGTCGTCGCCGAACAGGACGGCGAGGCGATCCTCTATAT
CGACCTGCACCTGATCCACGAGGTGACCACCCCGCAGGCCTTCGCGGGGCTGCGTGTCGCCGGCCGCCCGGTGCGCCGGC
CGGACCGCACCCTCGCCGTGGCCGACCACAACGTGCCGACCGAGGGGCAGGGCCTGGGGATCGACGCCGTCGCCGACGAG
GAGGCGCGCCTCCAGCTCCAGACCCTGGCGAAGAACGTCGCCGAGCACGGCATCGAGTTCTTCTCGATGGGCGACGTGCG
CAACGGCATCGTCCACGTGGTGGGGCCCGAACAGGGCCGCACCCAGCCGGGCATGACCATCGTCTGCGGCGATTCCCACA
CCTCGACCCACGGCGCCTTCGGGGCGCTGGCCCACGGCATCGGCACGTCCGAGGTCGAGCACGTCCTGGCCACCCAGACC
CTGCGCCAGAAGAAGGCGAAGAACATGCGGGTGCGCATCGAGGGCGAGCCCGCCCCCGGCGTCGGCGCGAAGGACTTCGC
CCTGGCGGTCATCGGCGAGATCGGCACCGCCGGCGGCACCGGCTACGTCATCGAGTACGCCGGCTCGGCCGTGCGGGCGC
TGTCGATGGAAGGCCGCATGACGCTGTGCAACCTGACCATCGAGGGCGGGGCCAAGGCGGGCCTGATCGCCCCGGACGAG
ATGACCTACGCCTATCTGCAGGGCCGGCCCGCCGCGCCGAAGGGCGGCGCCTGGGAGATGGCGCTGGACTACTGGAAGGG
CTTCCACAGCGACGAGGACGCCGTCTTCGACCGCGAGATCGTGCTGGACGCCGCGAAGATCGCCCCGACGGTGACCTGGG
GCACCAGCCCCGAGGACGTGGTGGCGGTCACCGGTCTCGTCCCCTCGCCCGACAGCTTCGGGACACCCGACAAGCGCGCC
TCGGCCGCCCGGGCCCTGGAGTACATGGGCCTGACCGCCGGCCAGCCGATCACCGAGGCGAAAGTCGACGTGGTCTTCAT
CGGCTCGTGCACCAACAGCCGGATCGAGGACCTGCGGGCGGCGGCGGCGATCGTCGCCAAGGCGCTGGAGGGCGGCCGCA
AGGTCGCCCCGGGCGTCCGGGCCATGGTCGTGCCGGGCTCGGGCCTGGTCCGCGAGCAGGCCGAGGCCGAGGGGCTGGAC
GAGGTGTTCAAGGCCGCCGGCTTCGACTGGCGCGAGCCGGGCTGCTCGATGTGCCTGGGCATGAACCCCGACCGCCTGGC
GCCGGGCGAGCGCTGCGCCTCGACCTCGAACCGCAACTTCGAGGGCCGCCAGGGCCGCGGCGGGCGCACCCATCTGATGA
GCCCGGCCATGGCCGCGGCGGCCGCGATCGCCGGCCACATCGCCGACGTGCGGGAGTTCCTGTGA

Upstream 100 bases:

>100_bases
CTTTCCCAATCGTCGCCGGGCAAGAAATTGCTGCAACCGCGTGCATTCTGCGGCGTCGCAGCACTAGACGTGCGCGGGTC
CGCGGACCTAGATGCGCGCC

Downstream 100 bases:

>100_bases
TCACCGGTCTCGACCACGTCGCGCTCTGCGTGCGCGACCTGGACGCGGCGGTGGACGGCTACCGCCGGCTGCTGGGCGTG
GAGCCCAACTGGCTGGGCGG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MPGKTLYDKIWDAHVVAEQDGEAILYIDLHLIHEVTTPQAFAGLRVAGRPVRRPDRTLAVADHNVPTEGQGLGIDAVADE
EARLQLQTLAKNVAEHGIEFFSMGDVRNGIVHVVGPEQGRTQPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LRQKKAKNMRVRIEGEPAPGVGAKDFALAVIGEIGTAGGTGYVIEYAGSAVRALSMEGRMTLCNLTIEGGAKAGLIAPDE
MTYAYLQGRPAAPKGGAWEMALDYWKGFHSDEDAVFDREIVLDAAKIAPTVTWGTSPEDVVAVTGLVPSPDSFGTPDKRA
SAARALEYMGLTAGQPITEAKVDVVFIGSCTNSRIEDLRAAAAIVAKALEGGRKVAPGVRAMVVPGSGLVREQAEAEGLD
EVFKAAGFDWREPGCSMCLGMNPDRLAPGERCASTSNRNFEGRQGRGGRTHLMSPAMAAAAAIAGHIADVREFL

Sequences:

>Translated_474_residues
MPGKTLYDKIWDAHVVAEQDGEAILYIDLHLIHEVTTPQAFAGLRVAGRPVRRPDRTLAVADHNVPTEGQGLGIDAVADE
EARLQLQTLAKNVAEHGIEFFSMGDVRNGIVHVVGPEQGRTQPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LRQKKAKNMRVRIEGEPAPGVGAKDFALAVIGEIGTAGGTGYVIEYAGSAVRALSMEGRMTLCNLTIEGGAKAGLIAPDE
MTYAYLQGRPAAPKGGAWEMALDYWKGFHSDEDAVFDREIVLDAAKIAPTVTWGTSPEDVVAVTGLVPSPDSFGTPDKRA
SAARALEYMGLTAGQPITEAKVDVVFIGSCTNSRIEDLRAAAAIVAKALEGGRKVAPGVRAMVVPGSGLVREQAEAEGLD
EVFKAAGFDWREPGCSMCLGMNPDRLAPGERCASTSNRNFEGRQGRGGRTHLMSPAMAAAAAIAGHIADVREFL
>Mature_473_residues
PGKTLYDKIWDAHVVAEQDGEAILYIDLHLIHEVTTPQAFAGLRVAGRPVRRPDRTLAVADHNVPTEGQGLGIDAVADEE
ARLQLQTLAKNVAEHGIEFFSMGDVRNGIVHVVGPEQGRTQPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
RQKKAKNMRVRIEGEPAPGVGAKDFALAVIGEIGTAGGTGYVIEYAGSAVRALSMEGRMTLCNLTIEGGAKAGLIAPDEM
TYAYLQGRPAAPKGGAWEMALDYWKGFHSDEDAVFDREIVLDAAKIAPTVTWGTSPEDVVAVTGLVPSPDSFGTPDKRAS
AARALEYMGLTAGQPITEAKVDVVFIGSCTNSRIEDLRAAAAIVAKALEGGRKVAPGVRAMVVPGSGLVREQAEAEGLDE
VFKAAGFDWREPGCSMCLGMNPDRLAPGERCASTSNRNFEGRQGRGGRTHLMSPAMAAAAAIAGHIADVREFL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI8659555, Length=402, Percent_Identity=26.865671641791, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI41352693, Length=379, Percent_Identity=27.7044854881267, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI4501867, Length=382, Percent_Identity=26.4397905759162, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1786259, Length=471, Percent_Identity=63.4819532908705, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI1787531, Length=448, Percent_Identity=24.7767857142857, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87081781, Length=362, Percent_Identity=25.9668508287293, Blast_Score=69, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=411, Percent_Identity=29.1970802919708, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=411, Percent_Identity=29.1970802919708, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=397, Percent_Identity=27.7078085642317, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI25149342, Length=318, Percent_Identity=29.874213836478, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321429, Length=475, Percent_Identity=61.0526315789474, Blast_Score=587, Evalue=1e-168,
Organism=Saccharomyces cerevisiae, GI6320440, Length=462, Percent_Identity=24.6753246753247, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=405, Percent_Identity=28.1481481481481, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6323335, Length=385, Percent_Identity=29.3506493506493, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI281365315, Length=384, Percent_Identity=28.3854166666667, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI17864292, Length=384, Percent_Identity=28.3854166666667, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI161076999, Length=384, Percent_Identity=28.3854166666667, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24645686, Length=370, Percent_Identity=27.2972972972973, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI17137564, Length=371, Percent_Identity=26.4150943396226, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28571643, Length=384, Percent_Identity=27.6041666666667, Blast_Score=101, Evalue=9e-22,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_PHEZH (B4RCC2)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002128950.1
- GeneID:   6789595
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0107
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 49994; Mature: 49863

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGKTLYDKIWDAHVVAEQDGEAILYIDLHLIHEVTTPQAFAGLRVAGRPVRRPDRTLAV
CCCCHHHHHHCCCEEEEECCCCEEEEEEEEEEHHHCCCHHHHCCEECCCCCCCCCCEEEE
ADHNVPTEGQGLGIDAVADEEARLQLQTLAKNVAEHGIEFFSMGDVRNGIVHVVGPEQGR
ECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCC
TQPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLRQKKAKNMRVRIEGEPAPG
CCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
VGAKDFALAVIGEIGTAGGTGYVIEYAGSAVRALSMEGRMTLCNLTIEGGAKAGLIAPDE
CCCCHHHHHEEHHCCCCCCCCEEEEECCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCC
MTYAYLQGRPAAPKGGAWEMALDYWKGFHSDEDAVFDREIVLDAAKIAPTVTWGTSPEDV
CEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCE
VAVTGLVPSPDSFGTPDKRASAARALEYMGLTAGQPITEAKVDVVFIGSCTNSRIEDLRA
EEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHHH
AAAIVAKALEGGRKVAPGVRAMVVPGSGLVREQAEAEGLDEVFKAAGFDWREPGCSMCLG
HHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCEEEC
MNPDRLAPGERCASTSNRNFEGRQGRGGRTHLMSPAMAAAAAIAGHIADVREFL
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
PGKTLYDKIWDAHVVAEQDGEAILYIDLHLIHEVTTPQAFAGLRVAGRPVRRPDRTLAV
CCCHHHHHHCCCEEEEECCCCEEEEEEEEEEHHHCCCHHHHCCEECCCCCCCCCCEEEE
ADHNVPTEGQGLGIDAVADEEARLQLQTLAKNVAEHGIEFFSMGDVRNGIVHVVGPEQGR
ECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCC
TQPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLRQKKAKNMRVRIEGEPAPG
CCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
VGAKDFALAVIGEIGTAGGTGYVIEYAGSAVRALSMEGRMTLCNLTIEGGAKAGLIAPDE
CCCCHHHHHEEHHCCCCCCCCEEEEECCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCC
MTYAYLQGRPAAPKGGAWEMALDYWKGFHSDEDAVFDREIVLDAAKIAPTVTWGTSPEDV
CEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCE
VAVTGLVPSPDSFGTPDKRASAARALEYMGLTAGQPITEAKVDVVFIGSCTNSRIEDLRA
EEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHHH
AAAIVAKALEGGRKVAPGVRAMVVPGSGLVREQAEAEGLDEVFKAAGFDWREPGCSMCLG
HHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCEEEC
MNPDRLAPGERCASTSNRNFEGRQGRGGRTHLMSPAMAAAAAIAGHIADVREFL
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA