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The map label for this gene is bioB

Identifier: 197103557

GI number: 197103557

Start: 85170

End: 86165

Strand: Reverse

Name: bioB

Synonym: PHZ_c0091

Alternate gene names: 197103557

Gene position: 86165-85170 (Counterclockwise)

Preceding gene: 197103564

Following gene: 197103556

Centisome position: 2.16

GC content: 70.08

Gene sequence:

>996_bases
ATGAACGCGCACGTCCGCCCTGTCGACCCCGCGAACGTCGATCCCGCCCAGCCGCGCCACGACTGGACGCTGGAGGAGGT
GGAGGCGCTGTTCGAGCTGCCCTTCACCGAGCTGGTGTTCCGGGCGGCCGAGGTCCACCGGCGCTGGTTCGACCCAACCG
AGCTGCAGCTCTCGCAGCTCCTGTCGGTGAAGACCGGCGGCTGTCCCGAGGACTGCGGCTACTGCGCCCAGTCGCAGAAG
TTCAAGACAGGGCTGGCGGCCTCGAAGCTGATGGAGGCCGAGACGGTGATCGCGGCCGCCGCCGAGGCCAAGGCGGGGGG
CGCCCAGCGCTTCTGCATGGGCGCGGCCTGGCGCGACCTGAAGGACCGCGACCTGCCGAAGGTCGCCGCCATGATCTCGG
GCGTGAAGGCCCTGGGCCTGGAGACCTGCGCCACCCTCGGCATGCTGACGGCCGACCAGGCGAAGGCGCTGAAGGCCGCC
GGCCTCGACTACTACAACCACAACCTCGACACCGGCCCGGACTACTACGACAAGGTCGTGACGACCCGCACCTACCAGGA
CCGGCTCGACACCCTGGCCGCCGTGCGCGACGCCGGCATGGCCACCTGCTGCGGCGGCATCGTCGGCATGGGCGAGACCC
GCCGCGACCGCGCGGGGCTGCTTCACCAGCTCGCCACCCTGCCCGCCCATCCGGACAGCCTGCCGATCAACGACCTGATG
CCGATCCCCGGAACCCCCCTGGGAGATTCCAAGGCGGTCGATCCCCTGGAGTTCGTGCGGATGATCGCCGTGGCGCGGAT
CGTCTGCCCGAAGTCGATGGTGCGCATCGCCGCCGGCCGCGAGCACATGACCAAGGAGCTGCAGGCCCTGTGCTTCCTGG
CCGGCGCCAATTCCATCTTCGTCGGCGCCCGGCTGCTGACCACCGACAATCCCGAGAAGACCGCCGACGAGGCCCTGCTC
GCGGACCTCAAGATGAAGCCCATGGCGATGGCCTGA

Upstream 100 bases:

>100_bases
AGCTCGAACTCGCGGCCCGCCTCGCCCATGCTGATCACCGCCCGTTGCTGACCCTGGGGAAAGCCCGGTAATGCGGGCTT
CCGGCCTCGGAGAGTTCCTC

Downstream 100 bases:

>100_bases
GGGCGGGAATCTGCCGCCCCGGCGGCAGATTCTTCACAGGGGAGCGCCGCCCTCGCAGGCAAGCGGTTGAAAACACGCCG
GTCTGCGGCGGCACGCCGCT

Product: biotin synthase BioB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNAHVRPVDPANVDPAQPRHDWTLEEVEALFELPFTELVFRAAEVHRRWFDPTELQLSQLLSVKTGGCPEDCGYCAQSQK
FKTGLAASKLMEAETVIAAAAEAKAGGAQRFCMGAAWRDLKDRDLPKVAAMISGVKALGLETCATLGMLTADQAKALKAA
GLDYYNHNLDTGPDYYDKVVTTRTYQDRLDTLAAVRDAGMATCCGGIVGMGETRRDRAGLLHQLATLPAHPDSLPINDLM
PIPGTPLGDSKAVDPLEFVRMIAVARIVCPKSMVRIAAGREHMTKELQALCFLAGANSIFVGARLLTTDNPEKTADEALL
ADLKMKPMAMA

Sequences:

>Translated_331_residues
MNAHVRPVDPANVDPAQPRHDWTLEEVEALFELPFTELVFRAAEVHRRWFDPTELQLSQLLSVKTGGCPEDCGYCAQSQK
FKTGLAASKLMEAETVIAAAAEAKAGGAQRFCMGAAWRDLKDRDLPKVAAMISGVKALGLETCATLGMLTADQAKALKAA
GLDYYNHNLDTGPDYYDKVVTTRTYQDRLDTLAAVRDAGMATCCGGIVGMGETRRDRAGLLHQLATLPAHPDSLPINDLM
PIPGTPLGDSKAVDPLEFVRMIAVARIVCPKSMVRIAAGREHMTKELQALCFLAGANSIFVGARLLTTDNPEKTADEALL
ADLKMKPMAMA
>Mature_331_residues
MNAHVRPVDPANVDPAQPRHDWTLEEVEALFELPFTELVFRAAEVHRRWFDPTELQLSQLLSVKTGGCPEDCGYCAQSQK
FKTGLAASKLMEAETVIAAAAEAKAGGAQRFCMGAAWRDLKDRDLPKVAAMISGVKALGLETCATLGMLTADQAKALKAA
GLDYYNHNLDTGPDYYDKVVTTRTYQDRLDTLAAVRDAGMATCCGGIVGMGETRRDRAGLLHQLATLPAHPDSLPINDLM
PIPGTPLGDSKAVDPLEFVRMIAVARIVCPKSMVRIAAGREHMTKELQALCFLAGANSIFVGARLLTTDNPEKTADEALL
ADLKMKPMAMA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=313, Percent_Identity=57.8274760383387, Blast_Score=394, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321725, Length=320, Percent_Identity=47.1875, Blast_Score=325, Evalue=7e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_PHEZH (B4RBP5)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002128934.1
- GeneID:   6789579
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0091
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2301681
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35755; Mature: 35755

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEEEECCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure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EEEEEEECCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA