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The map label for this gene is glpE [C]

Identifier: 197103533

GI number: 197103533

Start: 63394

End: 63756

Strand: Reverse

Name: glpE [C]

Synonym: PHZ_c0067

Alternate gene names: 197103533

Gene position: 63756-63394 (Counterclockwise)

Preceding gene: 197103539

Following gene: 197103532

Centisome position: 1.6

GC content: 72.18

Gene sequence:

>363_bases
ATGTTCGGACGCCAGAGGATCAAGGACCTGACCCCCAACGAGGTCAGGGCGAAGATGCAGCACCGGGAGATCGTGCTGAT
CGACGTGCGCGAGCCGCCCGAGTTCGCCGCCGAGCGCATCCATGGCGCGCTGAACTTCCCGCTGTCGACCTTCGACCCCA
GGGCCCTGCCCGCCGGCGGCGACCGCCCGCTGGTGTTCCAGTGCGGCTCGGGCAAGCGGTCGGCCATGGCTGTCGAGCGC
TGCCGGCAAGAAGGCGTGGCCGCCGAAGCGCACCTGGCCGGCGGCATCGCCGCCTGGCGCAGCGAGGGCCTGCCCACCAT
CCGCTTCGACCCGGCGACGGGCCAGGTCGTGGACGCCGGCTGA

Upstream 100 bases:

>100_bases
CCGGCCGCGGCGAGCCTAATGAAGCTGCCCTTGATCGGGCTTGATATATCAATATATGCGCAACTACGCATATACAAGCC
CATCAACCGGAGACGCCGCG

Downstream 100 bases:

>100_bases
CCCGCGATGATCCGCCCCCTCATCCCCGCAGCCCTCACGGCCGCCGCCCTGGCGCTGGCGGCCTGCGGCTCCGAGCCGAA
GCCGGCCGAGACCCCGGTGG

Product: rhodanese-related sulfurtransferase

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MFGRQRIKDLTPNEVRAKMQHREIVLIDVREPPEFAAERIHGALNFPLSTFDPRALPAGGDRPLVFQCGSGKRSAMAVER
CRQEGVAAEAHLAGGIAAWRSEGLPTIRFDPATGQVVDAG

Sequences:

>Translated_120_residues
MFGRQRIKDLTPNEVRAKMQHREIVLIDVREPPEFAAERIHGALNFPLSTFDPRALPAGGDRPLVFQCGSGKRSAMAVER
CRQEGVAAEAHLAGGIAAWRSEGLPTIRFDPATGQVVDAG
>Mature_120_residues
MFGRQRIKDLTPNEVRAKMQHREIVLIDVREPPEFAAERIHGALNFPLSTFDPRALPAGGDRPLVFQCGSGKRSAMAVER
CRQEGVAAEAHLAGGIAAWRSEGLPTIRFDPATGQVVDAG

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021309
- InterPro:   IPR001763 [H]

Pfam domain/function: PF11127 DUF2892; PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 13061; Mature: 13061

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGRQRIKDLTPNEVRAKMQHREIVLIDVREPPEFAAERIHGALNFPLSTFDPRALPAGG
CCCCHHHCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCC
DRPLVFQCGSGKRSAMAVERCRQEGVAAEAHLAGGIAAWRSEGLPTIRFDPATGQVVDAG
CCCEEEECCCCCCHHHHHHHHHHCCCCCHHHHHCCHHHHHCCCCCEEEECCCCCEEEECC
>Mature Secondary Structure
MFGRQRIKDLTPNEVRAKMQHREIVLIDVREPPEFAAERIHGALNFPLSTFDPRALPAGG
CCCCHHHCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCC
DRPLVFQCGSGKRSAMAVERCRQEGVAAEAHLAGGIAAWRSEGLPTIRFDPATGQVVDAG
CCCEEEECCCCCCHHHHHHHHHHCCCCCHHHHHCCHHHHHCCCCCEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]