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The map label for this gene is pksG [H]

Identifier: 195978200

GI number: 195978200

Start: 1114137

End: 1115312

Strand: Direct

Name: pksG [H]

Synonym: Sez_1079

Alternate gene names: 195978200

Gene position: 1114137-1115312 (Clockwise)

Preceding gene: 195978193

Following gene: 195978201

Centisome position: 55.04

GC content: 42.43

Gene sequence:

>1176_bases
ATGAACATCGGAATTGATAAGATTGGCTTTGCGACCAGTCAATACGTCTTAAAAATGGAAGACCTGGCACTTGCTCGCAG
CACTGATCCAGCAAAATTTCATCAGGGGCTAATGCTTGACGCTCTAAGCATTGCACCTGTTACAGAGGATATTGTAACGC
TTGCTGCATCAGCTGCTGACCAAATCCTTACCAAAGACGATAAAGCAAGCATTGATATGGTCATTTTAGCAACCGAATCA
AGCATTGATCAATCAAAGGCAGCCAGTGTTCACGTTCATCAGCTGCTTGATATTCAGCCCTTTGCACGCTCCTTTGAGAT
AAAAGAAGCCTGCTATAGTGCTACTGCTGCTTTAGATTATGCCAAATTACATGTTGCTGCACACCCAGCCTCCAAGGTCT
TGGTCATTGCTAGTGACATCGCCAAATACGGCATTGGATCAGCCGGAGAGCCAACACAAGGTGCTGGAAGTATTGCTATG
CTAGTCACTGCTAATCCTCGTATTCTCCAGTTAAATCATGACAACGTCGCACAGACACGTGATGTGATGGACTTTTGGCG
TCCAAATTACAGCTCAACACCATTTGTCAATGGTATTTATTCGACCAAGCAATACCTTGATTCCCTAAAAACAACCTGGG
ACAAATACCAAGAAAAAGAAGGTATTGAGCTATCAGACCTTGCTGCTGTTTGTTTTCATATCCCCTTTCCAAAGCTTGCT
CTAAAGGGACTTCATAAGATCATGACAAAATCACTAGCAGATGATCACAAAGAAAGGCTTTATGAAAACTTTCAAGCTTC
TATTGCCTACAACAAGCAAATCGGCAACATCTACACAGGCTCACTCTACCTCGGCTTATTATCACTTTTAGAGAATAGTC
AGGTATTAAGAGCTGGTGATCGGATTGGATTGTTTAGCTATGGCTCAGGAGCTGTTAGTGAGCTGTTTTCAGGGCAGCTT
GTTGACGGTTACAAGGACATGCTACTGACTAATCGACAAGCCCTATTAGACCAACGTCAAGTGATTTCAGTAGCTAATTA
TGAAGCACTTTTTTATGAAGAGCCTAGTCTAGATCAAGATGGCAATGCCAGCTTTTCACCCTATCTAACTGGCAGGTTTG
CCCTCAAAGAAATTAAGGAACACCAACGAATTTATCAAGATGATGACAAAAACTAA

Upstream 100 bases:

>100_bases
TTTGGTTTGAAAGATAAAACTATCGTATTAAATCAGCTAATTTTCTAAGAAAATATGATAAAATGGATAGGACTATAAAA
TGAGTAAAAGAAGGATATAT

Downstream 100 bases:

>100_bases
ACCAAATTGGGCCGGCTTTTATAAAAAAAGCCTTGCAGAACGCATTCAACAGCTCGCAGATGACCAGCTGCTTGGCCCGC
AGCAGCAAAAGCTGTTAGAG

Product: hydroxymethylglutaryl-CoA synthase

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: HMG synthase [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MNIGIDKIGFATSQYVLKMEDLALARSTDPAKFHQGLMLDALSIAPVTEDIVTLAASAADQILTKDDKASIDMVILATES
SIDQSKAASVHVHQLLDIQPFARSFEIKEACYSATAALDYAKLHVAAHPASKVLVIASDIAKYGIGSAGEPTQGAGSIAM
LVTANPRILQLNHDNVAQTRDVMDFWRPNYSSTPFVNGIYSTKQYLDSLKTTWDKYQEKEGIELSDLAAVCFHIPFPKLA
LKGLHKIMTKSLADDHKERLYENFQASIAYNKQIGNIYTGSLYLGLLSLLENSQVLRAGDRIGLFSYGSGAVSELFSGQL
VDGYKDMLLTNRQALLDQRQVISVANYEALFYEEPSLDQDGNASFSPYLTGRFALKEIKEHQRIYQDDDKN

Sequences:

>Translated_391_residues
MNIGIDKIGFATSQYVLKMEDLALARSTDPAKFHQGLMLDALSIAPVTEDIVTLAASAADQILTKDDKASIDMVILATES
SIDQSKAASVHVHQLLDIQPFARSFEIKEACYSATAALDYAKLHVAAHPASKVLVIASDIAKYGIGSAGEPTQGAGSIAM
LVTANPRILQLNHDNVAQTRDVMDFWRPNYSSTPFVNGIYSTKQYLDSLKTTWDKYQEKEGIELSDLAAVCFHIPFPKLA
LKGLHKIMTKSLADDHKERLYENFQASIAYNKQIGNIYTGSLYLGLLSLLENSQVLRAGDRIGLFSYGSGAVSELFSGQL
VDGYKDMLLTNRQALLDQRQVISVANYEALFYEEPSLDQDGNASFSPYLTGRFALKEIKEHQRIYQDDDKN
>Mature_391_residues
MNIGIDKIGFATSQYVLKMEDLALARSTDPAKFHQGLMLDALSIAPVTEDIVTLAASAADQILTKDDKASIDMVILATES
SIDQSKAASVHVHQLLDIQPFARSFEIKEACYSATAALDYAKLHVAAHPASKVLVIASDIAKYGIGSAGEPTQGAGSIAM
LVTANPRILQLNHDNVAQTRDVMDFWRPNYSSTPFVNGIYSTKQYLDSLKTTWDKYQEKEGIELSDLAAVCFHIPFPKLA
LKGLHKIMTKSLADDHKERLYENFQASIAYNKQIGNIYTGSLYLGLLSLLENSQVLRAGDRIGLFSYGSGAVSELFSGQL
VDGYKDMLLTNRQALLDQRQVISVANYEALFYEEPSLDQDGNASFSPYLTGRFALKEIKEHQRIYQDDDKN

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family [H]

Homologues:

Organism=Homo sapiens, GI5031751, Length=448, Percent_Identity=26.1160714285714, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI148298764, Length=364, Percent_Identity=27.7472527472527, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI148298677, Length=364, Percent_Identity=27.7472527472527, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17560126, Length=363, Percent_Identity=28.099173553719, Blast_Score=112, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323509, Length=406, Percent_Identity=25.615763546798, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24654139, Length=456, Percent_Identity=27.6315789473684, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24654137, Length=456, Percent_Identity=27.6315789473684, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24654133, Length=456, Percent_Identity=27.6315789473684, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24654135, Length=456, Percent_Identity=27.6315789473684, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI17933694, Length=456, Percent_Identity=27.6315789473684, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.3.10

Molecular weight: Translated: 43119; Mature: 43119

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIGIDKIGFATSQYVLKMEDLALARSTDPAKFHQGLMLDALSIAPVTEDIVTLAASAAD
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHH
QILTKDDKASIDMVILATESSIDQSKAASVHVHQLLDIQPFARSFEIKEACYSATAALDY
HHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AKLHVAAHPASKVLVIASDIAKYGIGSAGEPTQGAGSIAMLVTANPRILQLNHDNVAQTR
HHEEEEECCCCCEEEEEHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCHHHHH
DVMDFWRPNYSSTPFVNGIYSTKQYLDSLKTTWDKYQEKEGIELSDLAAVCFHIPFPKLA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHH
LKGLHKIMTKSLADDHKERLYENFQASIAYNKQIGNIYTGSLYLGLLSLLENSQVLRAGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEHHHHHHHHHHHCCCCHHHCCC
RIGLFSYGSGAVSELFSGQLVDGYKDMLLTNRQALLDQRQVISVANYEALFYEEPSLDQD
CEEEEECCCHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEEEECCCCCCC
GNASFSPYLTGRFALKEIKEHQRIYQDDDKN
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNIGIDKIGFATSQYVLKMEDLALARSTDPAKFHQGLMLDALSIAPVTEDIVTLAASAAD
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHH
QILTKDDKASIDMVILATESSIDQSKAASVHVHQLLDIQPFARSFEIKEACYSATAALDY
HHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AKLHVAAHPASKVLVIASDIAKYGIGSAGEPTQGAGSIAMLVTANPRILQLNHDNVAQTR
HHEEEEECCCCCEEEEEHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCHHHHH
DVMDFWRPNYSSTPFVNGIYSTKQYLDSLKTTWDKYQEKEGIELSDLAAVCFHIPFPKLA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHH
LKGLHKIMTKSLADDHKERLYENFQASIAYNKQIGNIYTGSLYLGLLSLLENSQVLRAGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEHHHHHHHHHHHCCCCHHHCCC
RIGLFSYGSGAVSELFSGQLVDGYKDMLLTNRQALLDQRQVISVANYEALFYEEPSLDQD
CEEEEECCCHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEEEECCCCCCC
GNASFSPYLTGRFALKEIKEHQRIYQDDDKN
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]