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The map label for this gene is rnhB

Identifier: 195978102

GI number: 195978102

Start: 1015511

End: 1016293

Strand: Direct

Name: rnhB

Synonym: Sez_0975

Alternate gene names: 195978102

Gene position: 1015511-1016293 (Clockwise)

Preceding gene: 195978101

Following gene: 195978103

Centisome position: 50.17

GC content: 43.42

Gene sequence:

>783_bases
ATGACAGCAATGACTATTAAAGACCTTAAAGTTCGTCTAGAAGCTGTTTCTAGTCTTTCTGATCCATTTTTTGAAGAGCT
AAAGGCGGATAAGCGGCTTGGCGTTCAAAAGGCTGTATCTAGCAGAAAGAGGGCTTTAGAGGCTATCTTAAAAGAGGAAG
CCCGGCTTGAGGCGATGTTGACGTATGAACAGGCGCTTTACCAAAAAGGCTTTCAAGCGATTGCTGGTATAGATGAGGTT
GGTCGAGGTCCTCTGGCTGGACCAATTGTAGCAGCCTGTGTTATACTGCCAAAGGGCTGCACCATTAAGGGGCTAAATGA
TTCTAAAAAAATTCCTAAAGCTAAGCATGAAGCAATTTATCAAGCCATTCAAAAGAAAGCGTTGGGAATTGGTCTTGGAA
TTATTGACAATCATACCATTGACCGGGTTAATATCTATGAAGCGACAAAATTAGCAATGATAGCTGCTATTAATCAGCTC
AAGGGCTCTGTGAGTAAGCCCGATTACCTGCTCATTGATGCCATGACACTAGAGGTTCCTATTCCTCAACAGGCTATTAT
AAAAGGCGATGCGAGCTCTATGTCAATTGCTGCAGCCTCTATTGTGGCTAAGGTTACCAGAGATCGATTGATGCTTGATT
ATGATAAGGCCTTTCCAGGCTATGGCTTTGCTAAAAATGCCGGCTATGGGACCAAAGAGCACCTGCAGGGATTACACCAG
TATGGTATCACACCGATTCATCGGCAAAGCTTTGAGCCGATAAAATCAATGACCTTACGCTAA

Upstream 100 bases:

>100_bases
CTTGGCTTTAAGGAAGACTATGATCGTTTTTACAGCTTATTCATCAAGGATATTCGTGATGGAAGACTTGGCCAGTACAC
CTTAGATCAGGTTGGTGATG

Downstream 100 bases:

>100_bases
AAAGCTTATTTGAAGGAAGGTAGCAGCCTGAAAGCTTTCGTAGCCTAAGAACGATAGTCACTAGGCGAATCTACAATTCT
GGTATTTTTCAATGGAATGC

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTAMTIKDLKVRLEAVSSLSDPFFEELKADKRLGVQKAVSSRKRALEAILKEEARLEAMLTYEQALYQKGFQAIAGIDEV
GRGPLAGPIVAACVILPKGCTIKGLNDSKKIPKAKHEAIYQAIQKKALGIGLGIIDNHTIDRVNIYEATKLAMIAAINQL
KGSVSKPDYLLIDAMTLEVPIPQQAIIKGDASSMSIAAASIVAKVTRDRLMLDYDKAFPGYGFAKNAGYGTKEHLQGLHQ
YGITPIHRQSFEPIKSMTLR

Sequences:

>Translated_260_residues
MTAMTIKDLKVRLEAVSSLSDPFFEELKADKRLGVQKAVSSRKRALEAILKEEARLEAMLTYEQALYQKGFQAIAGIDEV
GRGPLAGPIVAACVILPKGCTIKGLNDSKKIPKAKHEAIYQAIQKKALGIGLGIIDNHTIDRVNIYEATKLAMIAAINQL
KGSVSKPDYLLIDAMTLEVPIPQQAIIKGDASSMSIAAASIVAKVTRDRLMLDYDKAFPGYGFAKNAGYGTKEHLQGLHQ
YGITPIHRQSFEPIKSMTLR
>Mature_259_residues
TAMTIKDLKVRLEAVSSLSDPFFEELKADKRLGVQKAVSSRKRALEAILKEEARLEAMLTYEQALYQKGFQAIAGIDEVG
RGPLAGPIVAACVILPKGCTIKGLNDSKKIPKAKHEAIYQAIQKKALGIGLGIIDNHTIDRVNIYEATKLAMIAAINQLK
GSVSKPDYLLIDAMTLEVPIPQQAIIKGDASSMSIAAASIVAKVTRDRLMLDYDKAFPGYGFAKNAGYGTKEHLQGLHQY
GITPIHRQSFEPIKSMTLR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=185, Percent_Identity=48.1081081081081, Blast_Score=162, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_STREM (B4U2W6)

Other databases:

- EMBL:   CP001129
- RefSeq:   YP_002123346.1
- EnsemblBacteria:   EBSTRT00000008434
- GeneID:   6760726
- GenomeReviews:   CP001129_GR
- KEGG:   sez:Sez_0975
- GeneTree:   EBGT00050000028203
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 28365; Mature: 28233

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAMTIKDLKVRLEAVSSLSDPFFEELKADKRLGVQKAVSSRKRALEAILKEEARLEAML
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYEQALYQKGFQAIAGIDEVGRGPLAGPIVAACVILPKGCTIKGLNDSKKIPKAKHEAIY
HHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHH
QAIQKKALGIGLGIIDNHTIDRVNIYEATKLAMIAAINQLKGSVSKPDYLLIDAMTLEVP
HHHHHHHHHCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECC
IPQQAIIKGDASSMSIAAASIVAKVTRDRLMLDYDKAFPGYGFAKNAGYGTKEHLQGLHQ
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEHHCCCCCCCCCCCCCCCCHHHHHHHHH
YGITPIHRQSFEPIKSMTLR
HCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAMTIKDLKVRLEAVSSLSDPFFEELKADKRLGVQKAVSSRKRALEAILKEEARLEAML
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYEQALYQKGFQAIAGIDEVGRGPLAGPIVAACVILPKGCTIKGLNDSKKIPKAKHEAIY
HHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHH
QAIQKKALGIGLGIIDNHTIDRVNIYEATKLAMIAAINQLKGSVSKPDYLLIDAMTLEVP
HHHHHHHHHCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECC
IPQQAIIKGDASSMSIAAASIVAKVTRDRLMLDYDKAFPGYGFAKNAGYGTKEHLQGLHQ
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEHHCCCCCCCCCCCCCCCCHHHHHHHHH
YGITPIHRQSFEPIKSMTLR
HCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA