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The map label for this gene is dnaG [H]

Identifier: 195977962

GI number: 195977962

Start: 876928

End: 878739

Strand: Direct

Name: dnaG [H]

Synonym: Sez_0834

Alternate gene names: 195977962

Gene position: 876928-878739 (Clockwise)

Preceding gene: 195977960

Following gene: 195977963

Centisome position: 43.32

GC content: 42.77

Gene sequence:

>1812_bases
ATGGGCTTTTTGTGGGGAGGTGACGGTTTGGCTATTGACAAAGAGATGATTTCTCAGATCAAGCATAATGTCAATATTGT
TGATGTTATTGGAGAAGTTGTCCATCTATCGCGTACTGGTCGGCACTATCTTGGGCTGTGTCCCTTTCATAAAGAAAAAA
CACCCTCTTTTAATGTCATCGAAGACAGACAGTTCTTTCACTGCTTTGGCTGCGGTAAGTCGGGGGATGTTTTTAAGTTT
ATTGAGGAATATCGTCAGGTTCCTTTTTTAGAAAGTGTTCAGATTGTGGCTGATAAGGCCGGTATGGCAGTTACTATTGC
TCCAAGTCAGGCTAGCTTAGGCCAAAAGCAGCACCCCCACCAGGCTTTAATTGACCTTCATGAAGATGCTGCTAAGTTTT
ATCATGCGGTTTTGATGACGACAAAGATTGGTCAAAGAGCTAAGGAGTATCTTTATCAAAGAGGGCTTGATGATCAGCTA
ATAGAGCATTTTCGGATTGGTTTGGCGCCTAACGAGATGGACTACCTTTATCAAGCCTTGTCAAAAAAGTATACTGAGGC
GCAATTAACAGCCTCAGGCTTGTTTCATGTTTCTGAACAATCAAATGCTGTTTATGATGCCTTTCGTCATCGAATCATGT
TTCCCTTATGTGATGATAAGGGCAATATGATTGCTTTTTCAGGGCGTATCTGGGCAAGAGAGGATATAGAAAAGCAGCAG
GCTAAGTATAAAAATTCAAGGGCTACGGTCTTATTTAACAAGTCCTATGAGCTTTACCACCTTGATAAGGCCAAGCCGAT
AATCGCTAAGACTCATGAGGTCTTTTTGATGGAAGGCTTTATGGACGTTATTGCAGCCTATCGGGCAGGCTATGGCAATG
CTATTGCCTCTATGGGGACAGCATTGACGCCAGAGCATGTTAGACACCTCAAGCAGCTGACACAAAGGGTTATATTGACC
TATGATGGTGATGACGCCGGGCAAAATGCTATTGCCAAGTCTCTTGAGCTGCTGACAGATTTTCAAGTGGAGATCGTGAA
AATACCAAATAGAATGGATCCTGATGAATTTGTTCAGCAGCATTCACCAGAGGCTCTAGCAAAATTGCTCCAGCACTCGC
GCATTAGCAGTGTTGAGTTTTTGATTGATCACCTGAAGCCTGAAAATATGGATAACCTGCAATCGCAGATTGCTTATGTG
GAGCATATATCACAGATTATTGCTCAAGCACCCTCAATCACCGCACAAAATTCGTATATTAATAAGGTGGCTGATTTGCT
GCCGGATTTTGATTATTTTCAGGTGGAGCAATCGGTAAATGCTTTAAGGCTGCAAGATAGACAAAGCTTACAAGGACAGA
CTAGTCAATCAAAAGCATCTCTTGTGACCTTACCGATTAACAAAAGCATGACAGCCTTGGCTAAAACAGAAAGTCACCTC
CTGCATCGCTTGCTTCATCACGACTATTTGCTCAATGAATTTCGTCATCGGCCGGATTTTTATTTTGATACACCAGCCCT
TCAGACGCTGTATGAGCAGCTAAAGGCCACAGGGCAGATTACCTCCTATGACCTATCGCAACTGTCTGAAGAGGTTAACC
AAGCCTATTACCGGGTTTTAGAGGAAAATCTTCCTGATGAGATAGCTAGCGGTGAGATTGAGGATATTTTATCAAGGCAA
ATGACATTGCTAAAGCAAAGAGAGCTGCATAGGCAAGGAAAGCAGATTAGGGAATCTAGCAGTAAGGGTGACCACCAGAT
GGCTCTAGAGGCTTTAGAGCGTTTCATTGCTGAAAAAAGAAAAATGGAATAG

Upstream 100 bases:

>100_bases
TAGCTTATTGCTTGATGTAGTAGGCTTTAGATGATGAAGGATTGTGCTTTTACAATTTACAAAGCCCACTTTCAATGGTA
TAATGAAATGTAAATTTACA

Downstream 100 bases:

>100_bases
AGGAAAATATGGCAAAAGAAAAAGAAGTTACAACCTTTAACGTTCAAGTTGCGGAGTTTATTCGTCATCATAAAAAGGAA
GGAACAGCTACTGATGATGA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MGFLWGGDGLAIDKEMISQIKHNVNIVDVIGEVVHLSRTGRHYLGLCPFHKEKTPSFNVIEDRQFFHCFGCGKSGDVFKF
IEEYRQVPFLESVQIVADKAGMAVTIAPSQASLGQKQHPHQALIDLHEDAAKFYHAVLMTTKIGQRAKEYLYQRGLDDQL
IEHFRIGLAPNEMDYLYQALSKKYTEAQLTASGLFHVSEQSNAVYDAFRHRIMFPLCDDKGNMIAFSGRIWAREDIEKQQ
AKYKNSRATVLFNKSYELYHLDKAKPIIAKTHEVFLMEGFMDVIAAYRAGYGNAIASMGTALTPEHVRHLKQLTQRVILT
YDGDDAGQNAIAKSLELLTDFQVEIVKIPNRMDPDEFVQQHSPEALAKLLQHSRISSVEFLIDHLKPENMDNLQSQIAYV
EHISQIIAQAPSITAQNSYINKVADLLPDFDYFQVEQSVNALRLQDRQSLQGQTSQSKASLVTLPINKSMTALAKTESHL
LHRLLHHDYLLNEFRHRPDFYFDTPALQTLYEQLKATGQITSYDLSQLSEEVNQAYYRVLEENLPDEIASGEIEDILSRQ
MTLLKQRELHRQGKQIRESSSKGDHQMALEALERFIAEKRKME

Sequences:

>Translated_603_residues
MGFLWGGDGLAIDKEMISQIKHNVNIVDVIGEVVHLSRTGRHYLGLCPFHKEKTPSFNVIEDRQFFHCFGCGKSGDVFKF
IEEYRQVPFLESVQIVADKAGMAVTIAPSQASLGQKQHPHQALIDLHEDAAKFYHAVLMTTKIGQRAKEYLYQRGLDDQL
IEHFRIGLAPNEMDYLYQALSKKYTEAQLTASGLFHVSEQSNAVYDAFRHRIMFPLCDDKGNMIAFSGRIWAREDIEKQQ
AKYKNSRATVLFNKSYELYHLDKAKPIIAKTHEVFLMEGFMDVIAAYRAGYGNAIASMGTALTPEHVRHLKQLTQRVILT
YDGDDAGQNAIAKSLELLTDFQVEIVKIPNRMDPDEFVQQHSPEALAKLLQHSRISSVEFLIDHLKPENMDNLQSQIAYV
EHISQIIAQAPSITAQNSYINKVADLLPDFDYFQVEQSVNALRLQDRQSLQGQTSQSKASLVTLPINKSMTALAKTESHL
LHRLLHHDYLLNEFRHRPDFYFDTPALQTLYEQLKATGQITSYDLSQLSEEVNQAYYRVLEENLPDEIASGEIEDILSRQ
MTLLKQRELHRQGKQIRESSSKGDHQMALEALERFIAEKRKME
>Mature_602_residues
GFLWGGDGLAIDKEMISQIKHNVNIVDVIGEVVHLSRTGRHYLGLCPFHKEKTPSFNVIEDRQFFHCFGCGKSGDVFKFI
EEYRQVPFLESVQIVADKAGMAVTIAPSQASLGQKQHPHQALIDLHEDAAKFYHAVLMTTKIGQRAKEYLYQRGLDDQLI
EHFRIGLAPNEMDYLYQALSKKYTEAQLTASGLFHVSEQSNAVYDAFRHRIMFPLCDDKGNMIAFSGRIWAREDIEKQQA
KYKNSRATVLFNKSYELYHLDKAKPIIAKTHEVFLMEGFMDVIAAYRAGYGNAIASMGTALTPEHVRHLKQLTQRVILTY
DGDDAGQNAIAKSLELLTDFQVEIVKIPNRMDPDEFVQQHSPEALAKLLQHSRISSVEFLIDHLKPENMDNLQSQIAYVE
HISQIIAQAPSITAQNSYINKVADLLPDFDYFQVEQSVNALRLQDRQSLQGQTSQSKASLVTLPINKSMTALAKTESHLL
HRLLHHDYLLNEFRHRPDFYFDTPALQTLYEQLKATGQITSYDLSQLSEEVNQAYYRVLEENLPDEIASGEIEDILSRQM
TLLKQRELHRQGKQIRESSSKGDHQMALEALERFIAEKRKME

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=387, Percent_Identity=34.625322997416, Blast_Score=223, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68888; Mature: 68757

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLWGGDGLAIDKEMISQIKHNVNIVDVIGEVVHLSRTGRHYLGLCPFHKEKTPSFNVI
CCEEECCCCCEECHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEE
EDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIVADKAGMAVTIAPSQASLGQKQHPH
CCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCHHHCCCCCCCH
QALIDLHEDAAKFYHAVLMTTKIGQRAKEYLYQRGLDDQLIEHFRIGLAPNEMDYLYQAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHH
SKKYTEAQLTASGLFHVSEQSNAVYDAFRHRIMFPLCDDKGNMIAFSGRIWAREDIEKQQ
HHHHHHHHHHHHHHEEECCCCCHHHHHHHHHEECEEECCCCCEEEEECCCCCHHHHHHHH
AKYKNSRATVLFNKSYELYHLDKAKPIIAKTHEVFLMEGFMDVIAAYRAGYGNAIASMGT
HHHCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
ALTPEHVRHLKQLTQRVILTYDGDDAGQNAIAKSLELLTDFQVEIVKIPNRMDPDEFVQQ
CCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHC
HSPEALAKLLQHSRISSVEFLIDHLKPENMDNLQSQIAYVEHISQIIAQAPSITAQNSYI
CCHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
NKVADLLPDFDYFQVEQSVNALRLQDRQSLQGQTSQSKASLVTLPINKSMTALAKTESHL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHH
LHRLLHHDYLLNEFRHRPDFYFDTPALQTLYEQLKATGQITSYDLSQLSEEVNQAYYRVL
HHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
EENLPDEIASGEIEDILSRQMTLLKQRELHRQGKQIRESSSKGDHQMALEALERFIAEKR
HHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KME
CCC
>Mature Secondary Structure 
GFLWGGDGLAIDKEMISQIKHNVNIVDVIGEVVHLSRTGRHYLGLCPFHKEKTPSFNVI
CEEECCCCCEECHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEE
EDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIVADKAGMAVTIAPSQASLGQKQHPH
CCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCHHHCCCCCCCH
QALIDLHEDAAKFYHAVLMTTKIGQRAKEYLYQRGLDDQLIEHFRIGLAPNEMDYLYQAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHH
SKKYTEAQLTASGLFHVSEQSNAVYDAFRHRIMFPLCDDKGNMIAFSGRIWAREDIEKQQ
HHHHHHHHHHHHHHEEECCCCCHHHHHHHHHEECEEECCCCCEEEEECCCCCHHHHHHHH
AKYKNSRATVLFNKSYELYHLDKAKPIIAKTHEVFLMEGFMDVIAAYRAGYGNAIASMGT
HHHCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
ALTPEHVRHLKQLTQRVILTYDGDDAGQNAIAKSLELLTDFQVEIVKIPNRMDPDEFVQQ
CCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHC
HSPEALAKLLQHSRISSVEFLIDHLKPENMDNLQSQIAYVEHISQIIAQAPSITAQNSYI
CCHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
NKVADLLPDFDYFQVEQSVNALRLQDRQSLQGQTSQSKASLVTLPINKSMTALAKTESHL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHH
LHRLLHHDYLLNEFRHRPDFYFDTPALQTLYEQLKATGQITSYDLSQLSEEVNQAYYRVL
HHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
EENLPDEIASGEIEDILSRQMTLLKQRELHRQGKQIRESSSKGDHQMALEALERFIAEKR
HHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KME
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471; 7765979; 7503808 [H]