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The map label for this gene is pulA [H]

Identifier: 195977915

GI number: 195977915

Start: 816024

End: 818324

Strand: Direct

Name: pulA [H]

Synonym: Sez_0786

Alternate gene names: 195977915

Gene position: 816024-818324 (Clockwise)

Preceding gene: 195977914

Following gene: 195977916

Centisome position: 40.31

GC content: 43.5

Gene sequence:

>2301_bases
ATGGATAATGCAGTTATTGTTCATTATCATAGTTGCAAGGGACATTATTTTCAATTAAGTCTATGGCAATGGCGAGATGG
TAAGCTGGGCAAGGACGCTTATTTTTCGAGATTTGATAGCTTTGGAGCAGTTGCTTATCTCACTTATCCAGCACCTTATT
TTTTGAGCCATGCTTATGTGATTGTAAAGGATCAGTTTTGGCATTATCAAACCGTTGATTTTCGGATTGAGCGTGATTAT
GGGGTTCCAAAGACTGAGGTATGGCTTGTTGATGGTGATCCTACGGTTTATTATTCGAGACAGGCTGCTGTGGCTAGTCG
GCATTATAGCAGGTGTGATGTGCATGCCTTTGATATGGCTATTAACAGCAAGGCCTTTGATAAGCGCTGGGGCTTTAGTG
GCTGGCTAGGCTTTCGTTATCAGCCTGAAGAGACGTCTTTTCGTTTGTGGGCCCCAACAGCAGAAAAGGTCGAACTCATT
CTTTATGCTTCAACCGATGAGAGGGCTAGTGTTGCCAAGGTTTTACCCATGCAGCGCGGGCAGCAGTATTCGCCAGATCA
TCATGCAGAAAACACCTGTGGCGTGTGGGACATTAGCCTAAGAGGTGATTACAATTACCACGCTTATTGCTACCGAGTTT
ATTATCGCAGGCGTACCTTTAGAGATACGAGGGATCCTTATGCGATTGCGACAACAGCAAATGGTAAAAGGTCAATCGTC
ATTGCACCGGAGCATTTAAAGCCACAAGACTTTTCAGTGAAGCAGGGTAAGGAGGCAACCTGGCGCTTAGACAATCCTAA
TCAGGCTGTGATCTATGAAATGCATGTCCGTGACTTTTCAAAATCAGAAACCTCAGGCGTTAGCTTGGCTAATCGTGGCA
AATTTAAGGGGCTGATTGAGACAGGCACGCGCAATGCCTTTGGAGATTCAACCTGCTTTGATTATGTCAAGTCTCTTGGC
ATTACGCATATTCAGCTCCAGCCAATCTTTGATCATCATCAATTCTTTGATGACAATGGTGATTATGCCTACAACTGGGG
CTATGACCCTGAAAATTACAATGTTCCAGCAGCCAGCTTTACCAGTAATCCGCATGAGCCAGCTACCCGTATCTTGGAAT
TAAAGGAGGTCATTCAGACTTATCATGATGCAGGCATCAATGTGATCATGGACGTGGTGTATAATCATACCTACTCATCA
AGAGAGTCAGCCTTTCAGCTGACGGTTCCAGATTATTATTACCGTATGAATCCTAATGGCTCCTTTCAAAATGGCTCTGG
CTGTGGCAATGAAACGGCTAGCGAAAAGGAAATGTATCGCAAATACATGCTTGATTCGATCCTATATTGGACCAATGAGT
TCAACATTGATGGCTTTCGGTTTGATTTGATGGGCTTACACGATATTGATACCATGAACCTTATCCGGCAGGAGCTAGAT
AAGATTGACCCAAGGATACTTGTTTTTGGTGAGGGCTGGGACATGGGAGTTGGTCTGGCAGCTGAGCAGAAGGCTAAAAA
AGAAAATGCTAGTAAAATGCCTGGCATCGGCTTTTTTAATGATGATCAGCGTAATGCGGTTAAGGGGGCAGAGGTTTATG
GTAGCTTTGAGAAGGGCTTTGTATCAGGTGCACCGACAGAAGGACTTGTAGCCAAGTCTATTTTGGGCAGTGATGAGCTG
GTTTCTTATGGTACACCTAGTCAGGTGATTAATTATGTTGAAGCCCATGACAACTATAACCTCAATGATTTACTGTGGGT
CTTGAATCCAGAGGACAGCAAGCAGGATCATGTCAAACGGGTTCAGCTAGCCTCAGCCATGACGATTCTCATGCAAGGGA
TTTACTTTATGCAGCTTGGTCAGGAATTTTTACGAACAAAGCTTTACCCAACAGGTCAGGATAAGGAGCTGACACAGGCA
GACCGAGAAAGGGCGATGAATTCCTACAATGCACCTGACCATGTGAATCAGGTTGACTGGAAACAGGTTACCTTGGAGCG
AGAAACAGTCGCCATCACGAAAAAGCTAATCTATCTGAAAACCAAAACAGCTTTGTTTTCATACCCATCATTTGAACAAA
TCAGACAGCATGTTTACATTGAGCATGCTGACGATAATACCGGCTTTATTAGCTTTACAGTAGAAGATGAAAGAAAGTAT
CGGTTGATTTTCTCTAATTTTAGAAACCGCTTGCAATCACCTGCTAATCATGTTATAATTGAAACAAATGATAAGCGCTT
TCAACATAGCGAATCAATGCTAGAGGATTTGACAGCGATGCTCCTTGACATCACAAAATAG

Upstream 100 bases:

>100_bases
TTGTCCTAGACCAGGAGGAGCTGGAAATCGAAGAAATGGTCAAGAAATTTGCGCATGAGGTAGAGGATTTAGAGCAGGGA
TTGGAGGAATAGGGCTAGTG

Downstream 100 bases:

>100_bases
CCTGAGTGTTCTCAGGTTATTTTTGTTTGTTGTCGAGGATAAAAGGGCTGTAGTCACAGTTAGCAGAGACCTATTAGGAC
CTTGCTTATCATATCGCTTA

Product: glycogen debranching enzyme / pullulanase PulA

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]

Number of amino acids: Translated: 766; Mature: 766

Protein sequence:

>766_residues
MDNAVIVHYHSCKGHYFQLSLWQWRDGKLGKDAYFSRFDSFGAVAYLTYPAPYFLSHAYVIVKDQFWHYQTVDFRIERDY
GVPKTEVWLVDGDPTVYYSRQAAVASRHYSRCDVHAFDMAINSKAFDKRWGFSGWLGFRYQPEETSFRLWAPTAEKVELI
LYASTDERASVAKVLPMQRGQQYSPDHHAENTCGVWDISLRGDYNYHAYCYRVYYRRRTFRDTRDPYAIATTANGKRSIV
IAPEHLKPQDFSVKQGKEATWRLDNPNQAVIYEMHVRDFSKSETSGVSLANRGKFKGLIETGTRNAFGDSTCFDYVKSLG
ITHIQLQPIFDHHQFFDDNGDYAYNWGYDPENYNVPAASFTSNPHEPATRILELKEVIQTYHDAGINVIMDVVYNHTYSS
RESAFQLTVPDYYYRMNPNGSFQNGSGCGNETASEKEMYRKYMLDSILYWTNEFNIDGFRFDLMGLHDIDTMNLIRQELD
KIDPRILVFGEGWDMGVGLAAEQKAKKENASKMPGIGFFNDDQRNAVKGAEVYGSFEKGFVSGAPTEGLVAKSILGSDEL
VSYGTPSQVINYVEAHDNYNLNDLLWVLNPEDSKQDHVKRVQLASAMTILMQGIYFMQLGQEFLRTKLYPTGQDKELTQA
DRERAMNSYNAPDHVNQVDWKQVTLERETVAITKKLIYLKTKTALFSYPSFEQIRQHVYIEHADDNTGFISFTVEDERKY
RLIFSNFRNRLQSPANHVIIETNDKRFQHSESMLEDLTAMLLDITK

Sequences:

>Translated_766_residues
MDNAVIVHYHSCKGHYFQLSLWQWRDGKLGKDAYFSRFDSFGAVAYLTYPAPYFLSHAYVIVKDQFWHYQTVDFRIERDY
GVPKTEVWLVDGDPTVYYSRQAAVASRHYSRCDVHAFDMAINSKAFDKRWGFSGWLGFRYQPEETSFRLWAPTAEKVELI
LYASTDERASVAKVLPMQRGQQYSPDHHAENTCGVWDISLRGDYNYHAYCYRVYYRRRTFRDTRDPYAIATTANGKRSIV
IAPEHLKPQDFSVKQGKEATWRLDNPNQAVIYEMHVRDFSKSETSGVSLANRGKFKGLIETGTRNAFGDSTCFDYVKSLG
ITHIQLQPIFDHHQFFDDNGDYAYNWGYDPENYNVPAASFTSNPHEPATRILELKEVIQTYHDAGINVIMDVVYNHTYSS
RESAFQLTVPDYYYRMNPNGSFQNGSGCGNETASEKEMYRKYMLDSILYWTNEFNIDGFRFDLMGLHDIDTMNLIRQELD
KIDPRILVFGEGWDMGVGLAAEQKAKKENASKMPGIGFFNDDQRNAVKGAEVYGSFEKGFVSGAPTEGLVAKSILGSDEL
VSYGTPSQVINYVEAHDNYNLNDLLWVLNPEDSKQDHVKRVQLASAMTILMQGIYFMQLGQEFLRTKLYPTGQDKELTQA
DRERAMNSYNAPDHVNQVDWKQVTLERETVAITKKLIYLKTKTALFSYPSFEQIRQHVYIEHADDNTGFISFTVEDERKY
RLIFSNFRNRLQSPANHVIIETNDKRFQHSESMLEDLTAMLLDITK
>Mature_766_residues
MDNAVIVHYHSCKGHYFQLSLWQWRDGKLGKDAYFSRFDSFGAVAYLTYPAPYFLSHAYVIVKDQFWHYQTVDFRIERDY
GVPKTEVWLVDGDPTVYYSRQAAVASRHYSRCDVHAFDMAINSKAFDKRWGFSGWLGFRYQPEETSFRLWAPTAEKVELI
LYASTDERASVAKVLPMQRGQQYSPDHHAENTCGVWDISLRGDYNYHAYCYRVYYRRRTFRDTRDPYAIATTANGKRSIV
IAPEHLKPQDFSVKQGKEATWRLDNPNQAVIYEMHVRDFSKSETSGVSLANRGKFKGLIETGTRNAFGDSTCFDYVKSLG
ITHIQLQPIFDHHQFFDDNGDYAYNWGYDPENYNVPAASFTSNPHEPATRILELKEVIQTYHDAGINVIMDVVYNHTYSS
RESAFQLTVPDYYYRMNPNGSFQNGSGCGNETASEKEMYRKYMLDSILYWTNEFNIDGFRFDLMGLHDIDTMNLIRQELD
KIDPRILVFGEGWDMGVGLAAEQKAKKENASKMPGIGFFNDDQRNAVKGAEVYGSFEKGFVSGAPTEGLVAKSILGSDEL
VSYGTPSQVINYVEAHDNYNLNDLLWVLNPEDSKQDHVKRVQLASAMTILMQGIYFMQLGQEFLRTKLYPTGQDKELTQA
DRERAMNSYNAPDHVNQVDWKQVTLERETVAITKKLIYLKTKTALFSYPSFEQIRQHVYIEHADDNTGFISFTVEDERKY
RLIFSNFRNRLQSPANHVIIETNDKRFQHSESMLEDLTAMLLDITK

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=492, Percent_Identity=24.5934959349593, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI1789839, Length=319, Percent_Identity=22.884012539185, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR011840 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.41 [H]

Molecular weight: Translated: 88509; Mature: 88509

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNAVIVHYHSCKGHYFQLSLWQWRDGKLGKDAYFSRFDSFGAVAYLTYPAPYFLSHAYV
CCCCEEEEEECCCCEEEEEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCHHHHCCEEE
IVKDQFWHYQTVDFRIERDYGVPKTEVWLVDGDPTVYYSRQAAVASRHYSRCDVHAFDMA
EEEECCCEEEEEEEEEECCCCCCCEEEEEECCCCEEEEECHHHHHHHCCCCCCEEEEEEE
INSKAFDKRWGFSGWLGFRYQPEETSFRLWAPTAEKVELILYASTDERASVAKVLPMQRG
ECCCHHHHCCCCCCCCCEEECCCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHCC
QQYSPDHHAENTCGVWDISLRGDYNYHAYCYRVYYRRRTFRDTRDPYAIATTANGKRSIV
CCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEEEHHHHHCCCCCCCEEEEEECCCCEEEE
IAPEHLKPQDFSVKQGKEATWRLDNPNQAVIYEMHVRDFSKSETSGVSLANRGKFKGLIE
ECCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCCEECCCCCCCEEEE
TGTRNAFGDSTCFDYVKSLGITHIQLQPIFDHHQFFDDNGDYAYNWGYDPENYNVPAASF
CCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHCCCCCCEEEECCCCCCCCCCCCCCC
TSNPHEPATRILELKEVIQTYHDAGINVIMDVVYNHTYSSRESAFQLTVPDYYYRMNPNG
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCCC
SFQNGSGCGNETASEKEMYRKYMLDSILYWTNEFNIDGFRFDLMGLHDIDTMNLIRQELD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
KIDPRILVFGEGWDMGVGLAAEQKAKKENASKMPGIGFFNDDQRNAVKGAEVYGSFEKGF
HCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHCC
VSGAPTEGLVAKSILGSDELVSYGTPSQVINYVEAHDNYNLNDLLWVLNPEDSKQDHVKR
CCCCCCCCHHHHHHCCCCCEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHH
VQLASAMTILMQGIYFMQLGQEFLRTKLYPTGQDKELTQADRERAMNSYNAPDHVNQVDW
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCH
KQVTLERETVAITKKLIYLKTKTALFSYPSFEQIRQHVYIEHADDNTGFISFTVEDERKY
HEEEECHHHHHEEEEEEEEEEHHHEECCCCHHHHHHEEEEEECCCCCCEEEEEEECCCEE
RLIFSNFRNRLQSPANHVIIETNDKRFQHSESMLEDLTAMLLDITK
EHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDNAVIVHYHSCKGHYFQLSLWQWRDGKLGKDAYFSRFDSFGAVAYLTYPAPYFLSHAYV
CCCCEEEEEECCCCEEEEEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCHHHHCCEEE
IVKDQFWHYQTVDFRIERDYGVPKTEVWLVDGDPTVYYSRQAAVASRHYSRCDVHAFDMA
EEEECCCEEEEEEEEEECCCCCCCEEEEEECCCCEEEEECHHHHHHHCCCCCCEEEEEEE
INSKAFDKRWGFSGWLGFRYQPEETSFRLWAPTAEKVELILYASTDERASVAKVLPMQRG
ECCCHHHHCCCCCCCCCEEECCCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHCC
QQYSPDHHAENTCGVWDISLRGDYNYHAYCYRVYYRRRTFRDTRDPYAIATTANGKRSIV
CCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEEEHHHHHCCCCCCCEEEEEECCCCEEEE
IAPEHLKPQDFSVKQGKEATWRLDNPNQAVIYEMHVRDFSKSETSGVSLANRGKFKGLIE
ECCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCCEECCCCCCCEEEE
TGTRNAFGDSTCFDYVKSLGITHIQLQPIFDHHQFFDDNGDYAYNWGYDPENYNVPAASF
CCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHCCCCCCEEEECCCCCCCCCCCCCCC
TSNPHEPATRILELKEVIQTYHDAGINVIMDVVYNHTYSSRESAFQLTVPDYYYRMNPNG
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCCC
SFQNGSGCGNETASEKEMYRKYMLDSILYWTNEFNIDGFRFDLMGLHDIDTMNLIRQELD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
KIDPRILVFGEGWDMGVGLAAEQKAKKENASKMPGIGFFNDDQRNAVKGAEVYGSFEKGF
HCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHCC
VSGAPTEGLVAKSILGSDELVSYGTPSQVINYVEAHDNYNLNDLLWVLNPEDSKQDHVKR
CCCCCCCCHHHHHHCCCCCEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHH
VQLASAMTILMQGIYFMQLGQEFLRTKLYPTGQDKELTQADRERAMNSYNAPDHVNQVDW
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCH
KQVTLERETVAITKKLIYLKTKTALFSYPSFEQIRQHVYIEHADDNTGFISFTVEDERKY
HEEEECHHHHHEEEEEEEEEEHHHEECCCCHHHHHHEEEEEECCCCCCEEEEEEECCCEE
RLIFSNFRNRLQSPANHVIIETNDKRFQHSESMLEDLTAMLLDITK
EHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]