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The map label for this gene is alaS [H]

Identifier: 195977801

GI number: 195977801

Start: 689734

End: 692352

Strand: Direct

Name: alaS [H]

Synonym: Sez_0667

Alternate gene names: 195977801

Gene position: 689734-692352 (Clockwise)

Preceding gene: 195977800

Following gene: 195977804

Centisome position: 34.07

GC content: 44.71

Gene sequence:

>2619_bases
ATGAAACAATTAACATCAGCCCAAATCCGCCAAATGTGGCTTGATTTTTGGAAATCAAAGGGTCACTCTATTGAGCCTTC
GGCAAATCTTGTGCCGGTAAATGACCCAACCCTGCTTTGGATTAACTCTGGTGTGGCCACCCTCAAAAAATATTTTGATG
GGTCAGTCATTCCTGAAAATCCTCGTATCACCAATGCTCAAAAGTCAATCCGCACTAACGATATTGAAAATGTTGGTAAG
ACAGCTCGTCACCATACCATGTTTGAAATGCTTGGTAATTTTTCTATCGGAGATTATTTCCGCGATGAAGCAATTGAGTG
GGGCTTTGAATTGTTAACAAGTCCGGAATGGTTCGATTTTCCAAAGGAAAAATTATATATGACCTACTACCCAGATGACA
AGGACTCTTACAATCGTTGGATTGCTCTGGGCGTTGAGCCAAGTCACTTGATTCCACTAGAGGAAAACTTTTGGGAGATT
GGTGCAGGCCCGTCAGGACCTGATACAGAGATTTTCTTTGATCGTGGTGAGGCCTATGATCCTGATCATATTGGGATTAG
ATTGCTGGAAGAGGACATTGAAAACGACCGTTATATTGAAATTTGGAATATTGTTTTGTCTCAATTTAACGCAGACCCTA
CAATTCCTCGCTCAGAGTACAAGGAGCTTCCAAATAAAAATATTGATACGGGTGCTGGCTTAGAGCGTCTTGTGGCTGTT
ATGCAGGGTGCAAAGACAAACTTTGAAACAGACCTATTTATGCCGATCATCAAGGAAGTGGAAAAGCTATCTGGCCATAC
TTACGACCAAGATGGTGACAATATGAGCTTTAAGGTCATTGCAGACCACATTCGTGCCTTGTCATTTGCGATTGGAGATG
GTGCCCTACCTGGTAATGAAGGTCGTGGCTATGTGCTTCGTCGCTTGCTTCGTCGTGCTGTTATGCATGGTCGTAGGCTC
GGTATTCATGAGACCTTTCTTTATAAGCTAGTAGCTACTGTAGGTCATATCATGGAGTCCTATTATCCAGAGATTCTTGA
GAAGCGTGACTTTATTGAAAAAATTATCAAGCGTGAGGAGGAAACCTTTGCACGTACCATTGATGCAGGATCAGGGCATT
TGGATCAATTGCTTGCGCAGCTAAAAGCAAAGGGGCAGGATAGGCTTGATGGCAAGGATATCTTTAAGCTGTATGATACC
TATGGCTTTCCAGTTGAATTGACAGAAGAATTAGCAGAAGATGCTGGCTATAAGATTGATCATGAAGGCTTCAAGGCAGC
TATGAAGGAGCAGCAGGATCGTGCCCGTGCAGCAGCTGTTAAGGGTGGCTCAATGGGCATGCAAAATGAGACACTGGCAG
GCATCACAGAGCCATCAGAATTTTTGTACGAAACAGAGACTGTTGCCTCTCGCTTATCAGTTATCATCGTTGATAATGAG
CGTAGTGAGATGGTTTCAGAAGGACAGGCCTTGCTTGTGTTTGATCAGACACCGTTTTATGCTGAAATGGGTGGTCAGGT
TGCCGATCATGGGGTTATCAAAAATGATAAGGGAGACCCTGTTGCTCGTGTGATAGACGTGCAAAAAGCACCAAATGGTC
AGCCGCTCCACACTGTTGAGGTCCTTGCAAGCCTTGCATTAAATACTGTTTATACCCTTGCTATTGATCACAAGCGCCGC
TATGCTGTTGAGAAAAACCATACCGCTACGCACCTGCTTCATGCTGCCCTTCACAAGATTATCGGAGAGCATGCCACTCA
GGCTGGCTCCTTAAATGAAGAAGCCTTCTTACGCTTTGACTTTACGCACTTTGAAGCTGTTACAGCACAGGAACTTCGTC
GCATTGAAGAAGAGGTCAATGAGCAAATCTGGAAGGCATTAGCCATTACAACCACAGAAACTGATATTGAAACAGCAAAG
GCTATGGGAGCTATGGCCCTCTTTGGTGAAAAATATGGCAAAACTGTTCGTGTTGTTCAGATTGGCGATTATTCTGTTGA
GCTTTGTGGCGGGACTCACTTATCCAACTCATCAGAAATTGGTCTATTCAAGATTATCAAGGAAGAGGGAATCGGCTCAG
GAACACGTCGTATCATAGCAGTGACAGGTCAGCAGGCCTTTGAAAGCTTCCGCAAGCAAGAAGACACCCTAAAAGAAATT
GCACATACGCTTAAGGTGCCGCAAATAGAACAGCTTCCAAGTAAGGTTGTGAGCCTTAGCGAGCAGATCCGCGATTTGCA
AAAAGAAATAGCAGAGCTAAAAGAAAAAGCAGCAGCAGCTCAGGCTGGTGATGTCTTTAAAAAGGTCCAGGAAGCAAACG
ATCTACGTTACATTGCTAGTGAGGTATCCGTATCAGATGCAGGAGCCTTGCGTACCTTTGCCGATAATTGGAAGCAAAAG
GATTATTCAGATGTCCTTGTGCTGGCTGCAAGCATTGGTGAGAAGGTCAATGTTCTTGTTGCAAGTAAGGACAAAAGAGC
TCACGCTGGCAATCTGATTAAGGCCTTAGCGCCAATCGTGTCGGGTCGTGGTGGCGGTAAGCCAGATATGGCGATGGCAG
GTGGTAGTGATGCAAGCAAGCTATCAGAGCTGTTAGCCGCAGTACCAGAGCATTTATAA

Upstream 100 bases:

>100_bases
CAACGAAAGGAACTGAATGCGAGTAGAGATCGTCTCTCAAGGCTACTTATGCTAAGGACAATCATACCTAGTCTCTCTTA
TCGCTCTTGGTATCTTAATC

Downstream 100 bases:

>100_bases
AAGCGATAAGAGGTTGAGTGCTTTATCAGCCTCTTTATCTTTTAGCTAAGGAGCTATCTAATCCTTCATCACACAAAAAA
AGCTGGTTGCTTCAACCAGC

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 872; Mature: 872

Protein sequence:

>872_residues
MKQLTSAQIRQMWLDFWKSKGHSIEPSANLVPVNDPTLLWINSGVATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGK
TARHHTMFEMLGNFSIGDYFRDEAIEWGFELLTSPEWFDFPKEKLYMTYYPDDKDSYNRWIALGVEPSHLIPLEENFWEI
GAGPSGPDTEIFFDRGEAYDPDHIGIRLLEEDIENDRYIEIWNIVLSQFNADPTIPRSEYKELPNKNIDTGAGLERLVAV
MQGAKTNFETDLFMPIIKEVEKLSGHTYDQDGDNMSFKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRL
GIHETFLYKLVATVGHIMESYYPEILEKRDFIEKIIKREEETFARTIDAGSGHLDQLLAQLKAKGQDRLDGKDIFKLYDT
YGFPVELTEELAEDAGYKIDHEGFKAAMKEQQDRARAAAVKGGSMGMQNETLAGITEPSEFLYETETVASRLSVIIVDNE
RSEMVSEGQALLVFDQTPFYAEMGGQVADHGVIKNDKGDPVARVIDVQKAPNGQPLHTVEVLASLALNTVYTLAIDHKRR
YAVEKNHTATHLLHAALHKIIGEHATQAGSLNEEAFLRFDFTHFEAVTAQELRRIEEEVNEQIWKALAITTTETDIETAK
AMGAMALFGEKYGKTVRVVQIGDYSVELCGGTHLSNSSEIGLFKIIKEEGIGSGTRRIIAVTGQQAFESFRKQEDTLKEI
AHTLKVPQIEQLPSKVVSLSEQIRDLQKEIAELKEKAAAAQAGDVFKKVQEANDLRYIASEVSVSDAGALRTFADNWKQK
DYSDVLVLAASIGEKVNVLVASKDKRAHAGNLIKALAPIVSGRGGGKPDMAMAGGSDASKLSELLAAVPEHL

Sequences:

>Translated_872_residues
MKQLTSAQIRQMWLDFWKSKGHSIEPSANLVPVNDPTLLWINSGVATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGK
TARHHTMFEMLGNFSIGDYFRDEAIEWGFELLTSPEWFDFPKEKLYMTYYPDDKDSYNRWIALGVEPSHLIPLEENFWEI
GAGPSGPDTEIFFDRGEAYDPDHIGIRLLEEDIENDRYIEIWNIVLSQFNADPTIPRSEYKELPNKNIDTGAGLERLVAV
MQGAKTNFETDLFMPIIKEVEKLSGHTYDQDGDNMSFKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRL
GIHETFLYKLVATVGHIMESYYPEILEKRDFIEKIIKREEETFARTIDAGSGHLDQLLAQLKAKGQDRLDGKDIFKLYDT
YGFPVELTEELAEDAGYKIDHEGFKAAMKEQQDRARAAAVKGGSMGMQNETLAGITEPSEFLYETETVASRLSVIIVDNE
RSEMVSEGQALLVFDQTPFYAEMGGQVADHGVIKNDKGDPVARVIDVQKAPNGQPLHTVEVLASLALNTVYTLAIDHKRR
YAVEKNHTATHLLHAALHKIIGEHATQAGSLNEEAFLRFDFTHFEAVTAQELRRIEEEVNEQIWKALAITTTETDIETAK
AMGAMALFGEKYGKTVRVVQIGDYSVELCGGTHLSNSSEIGLFKIIKEEGIGSGTRRIIAVTGQQAFESFRKQEDTLKEI
AHTLKVPQIEQLPSKVVSLSEQIRDLQKEIAELKEKAAAAQAGDVFKKVQEANDLRYIASEVSVSDAGALRTFADNWKQK
DYSDVLVLAASIGEKVNVLVASKDKRAHAGNLIKALAPIVSGRGGGKPDMAMAGGSDASKLSELLAAVPEHL
>Mature_872_residues
MKQLTSAQIRQMWLDFWKSKGHSIEPSANLVPVNDPTLLWINSGVATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGK
TARHHTMFEMLGNFSIGDYFRDEAIEWGFELLTSPEWFDFPKEKLYMTYYPDDKDSYNRWIALGVEPSHLIPLEENFWEI
GAGPSGPDTEIFFDRGEAYDPDHIGIRLLEEDIENDRYIEIWNIVLSQFNADPTIPRSEYKELPNKNIDTGAGLERLVAV
MQGAKTNFETDLFMPIIKEVEKLSGHTYDQDGDNMSFKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRL
GIHETFLYKLVATVGHIMESYYPEILEKRDFIEKIIKREEETFARTIDAGSGHLDQLLAQLKAKGQDRLDGKDIFKLYDT
YGFPVELTEELAEDAGYKIDHEGFKAAMKEQQDRARAAAVKGGSMGMQNETLAGITEPSEFLYETETVASRLSVIIVDNE
RSEMVSEGQALLVFDQTPFYAEMGGQVADHGVIKNDKGDPVARVIDVQKAPNGQPLHTVEVLASLALNTVYTLAIDHKRR
YAVEKNHTATHLLHAALHKIIGEHATQAGSLNEEAFLRFDFTHFEAVTAQELRRIEEEVNEQIWKALAITTTETDIETAK
AMGAMALFGEKYGKTVRVVQIGDYSVELCGGTHLSNSSEIGLFKIIKEEGIGSGTRRIIAVTGQQAFESFRKQEDTLKEI
AHTLKVPQIEQLPSKVVSLSEQIRDLQKEIAELKEKAAAAQAGDVFKKVQEANDLRYIASEVSVSDAGALRTFADNWKQK
DYSDVLVLAASIGEKVNVLVASKDKRAHAGNLIKALAPIVSGRGGGKPDMAMAGGSDASKLSELLAAVPEHL

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=974, Percent_Identity=34.4969199178645, Blast_Score=461, Evalue=1e-129,
Organism=Homo sapiens, GI38569417, Length=821, Percent_Identity=31.5468940316687, Blast_Score=337, Evalue=4e-92,
Organism=Escherichia coli, GI1789048, Length=878, Percent_Identity=44.7608200455581, Blast_Score=696, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=969, Percent_Identity=31.4757481940144, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17536681, Length=744, Percent_Identity=32.5268817204301, Blast_Score=344, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6324911, Length=938, Percent_Identity=33.9019189765458, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24582809, Length=970, Percent_Identity=32.3711340206186, Blast_Score=454, Evalue=1e-127,
Organism=Drosophila melanogaster, GI45552267, Length=970, Percent_Identity=32.3711340206186, Blast_Score=454, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24658214, Length=808, Percent_Identity=27.970297029703, Blast_Score=265, Evalue=8e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR016040
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 97233; Mature: 97233

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLTSAQIRQMWLDFWKSKGHSIEPSANLVPVNDPTLLWINSGVATLKKYFDGSVIPEN
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHCCCCCCCC
PRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRDEAIEWGFELLTSPEWFDF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
PKEKLYMTYYPDDKDSYNRWIALGVEPSHLIPLEENFWEIGAGPSGPDTEIFFDRGEAYD
CCCCEEEEECCCCCCCCCCEEEEECCCCCCCCCHHCCEECCCCCCCCCCCEEEECCCCCC
PDHIGIRLLEEDIENDRYIEIWNIVLSQFNADPTIPRSEYKELPNKNIDTGAGLERLVAV
CCHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHH
MQGAKTNFETDLFMPIIKEVEKLSGHTYDQDGDNMSFKVIADHIRALSFAIGDGALPGNE
HHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
GRGYVLRRLLRRAVMHGRRLGIHETFLYKLVATVGHIMESYYPEILEKRDFIEKIIKREE
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETFARTIDAGSGHLDQLLAQLKAKGQDRLDGKDIFKLYDTYGFPVELTEELAEDAGYKID
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEC
HEGFKAAMKEQQDRARAAAVKGGSMGMQNETLAGITEPSEFLYETETVASRLSVIIVDNE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECC
RSEMVSEGQALLVFDQTPFYAEMGGQVADHGVIKNDKGDPVARVIDVQKAPNGQPLHTVE
HHHHHHCCCEEEEEECCCCHHHHCCCHHCCCCCCCCCCCHHHHHEEHHCCCCCCCHHHHH
VLASLALNTVYTLAIDHKRRYAVEKNHTATHLLHAALHKIIGEHATQAGSLNEEAFLRFD
HHHHHHHHHHHEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
FTHFEAVTAQELRRIEEEVNEQIWKALAITTTETDIETAKAMGAMALFGEKYGKTVRVVQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCEEEEEE
IGDYSVELCGGTHLSNSSEIGLFKIIKEEGIGSGTRRIIAVTGQQAFESFRKQEDTLKEI
ECCEEEEEECCCCCCCCCCCHHHEEHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
AHTLKVPQIEQLPSKVVSLSEQIRDLQKEIAELKEKAAAAQAGDVFKKVQEANDLRYIAS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVSVSDAGALRTFADNWKQKDYSDVLVLAASIGEKVNVLVASKDKRAHAGNLIKALAPIV
HCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
SGRGGGKPDMAMAGGSDASKLSELLAAVPEHL
CCCCCCCCCCEECCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKQLTSAQIRQMWLDFWKSKGHSIEPSANLVPVNDPTLLWINSGVATLKKYFDGSVIPEN
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHCCCCCCCC
PRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRDEAIEWGFELLTSPEWFDF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
PKEKLYMTYYPDDKDSYNRWIALGVEPSHLIPLEENFWEIGAGPSGPDTEIFFDRGEAYD
CCCCEEEEECCCCCCCCCCEEEEECCCCCCCCCHHCCEECCCCCCCCCCCEEEECCCCCC
PDHIGIRLLEEDIENDRYIEIWNIVLSQFNADPTIPRSEYKELPNKNIDTGAGLERLVAV
CCHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHH
MQGAKTNFETDLFMPIIKEVEKLSGHTYDQDGDNMSFKVIADHIRALSFAIGDGALPGNE
HHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
GRGYVLRRLLRRAVMHGRRLGIHETFLYKLVATVGHIMESYYPEILEKRDFIEKIIKREE
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETFARTIDAGSGHLDQLLAQLKAKGQDRLDGKDIFKLYDTYGFPVELTEELAEDAGYKID
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEC
HEGFKAAMKEQQDRARAAAVKGGSMGMQNETLAGITEPSEFLYETETVASRLSVIIVDNE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECC
RSEMVSEGQALLVFDQTPFYAEMGGQVADHGVIKNDKGDPVARVIDVQKAPNGQPLHTVE
HHHHHHCCCEEEEEECCCCHHHHCCCHHCCCCCCCCCCCHHHHHEEHHCCCCCCCHHHHH
VLASLALNTVYTLAIDHKRRYAVEKNHTATHLLHAALHKIIGEHATQAGSLNEEAFLRFD
HHHHHHHHHHHEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
FTHFEAVTAQELRRIEEEVNEQIWKALAITTTETDIETAKAMGAMALFGEKYGKTVRVVQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCEEEEEE
IGDYSVELCGGTHLSNSSEIGLFKIIKEEGIGSGTRRIIAVTGQQAFESFRKQEDTLKEI
ECCEEEEEECCCCCCCCCCCHHHEEHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
AHTLKVPQIEQLPSKVVSLSEQIRDLQKEIAELKEKAAAAQAGDVFKKVQEANDLRYIAS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVSVSDAGALRTFADNWKQKDYSDVLVLAASIGEKVNVLVASKDKRAHAGNLIKALAPIV
HCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
SGRGGGKPDMAMAGGSDASKLSELLAAVPEHL
CCCCCCCCCCEECCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA