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The map label for this gene is rlmN [H]

Identifier: 195977717

GI number: 195977717

Start: 605374

End: 606456

Strand: Direct

Name: rlmN [H]

Synonym: Sez_0579

Alternate gene names: 195977717

Gene position: 605374-606456 (Clockwise)

Preceding gene: 195977716

Following gene: 195977718

Centisome position: 29.91

GC content: 43.86

Gene sequence:

>1083_bases
ATGAAACCATCAATCTATGGCCTAACGCGCGACGAGCTAATTGCTTGGGCGATTGACAATGGTCAAAAGGCATTCAGAGC
GACACAGATTTGGGACTGGCTTTACAGAAAACGGATTCAATCCTTTGATGAAATGACTAATATTTCTAAGGAATTTCTTG
CCATTCTAAAGGACAGCTTTTGTATTAATCCCTTGAAGCAGCGTGTTGCTCAGGAATCAGCTGATGGAACGGTTAAGTAT
TTGTTTGAATTGCCAGATGGTATGCTGATTGAAACGGTTTTGATGCGTCAGCACTACGGGCAGTCGGTCTGTGTAACCAC
ACAGGTTGGCTGTAATATAGGCTGCACCTTCTGCGCTAGTGGCTTGATTAAAAAGCAGCGGGATTTAAACAGTGGTGAAA
TCACTGCACAAATTATGATGGTGCAAAACTATTTTGATCAGCGTGGCCAAGACGAACGCGTTAGCCATGTTGTTGTGATG
GGGATTGGAGAGCCCTTTGATAATTATCAAAATGTGATGACCTTTCTTCGGACGATCAATGATGATCATGGGCTTGCTAT
TGGTGCTCGTCACATTACTGTTTCAACCTCAGGCTTGGCTCACAAGATTCGCGAATTTGCCAATGAAGGAGTCCAAGTCA
ATCTGGCTGTGTCACTGCATGCACCTAATAATGAGCTGCGCTCAAGTATCATGAGGATCAATCGTTCCTTCCCACTTGAT
AAGCTCTTTTCAGCAATCGAATATTACATTGAAACAACTAATCGTCGTGTGACCTTTGAGTATATCATGCTTAATAAGGT
TAATGATGGGGTAGAGCAGGCACAGGAATTGGCAGATTTGACCAAAAGGATTCGCAAGCTGTCTTATGTTAACCTGATTC
CCTATAATCCTGTCTCAGAGCATGACCAATACAGTAGAAGTCCTAAGGAGCGTGTGGCAGCCTTTTATGACATTCTAAAG
AAAAATGGAGTGAACTGTGTCGTTCGTCAGGAGCATGGTACAGATATTGATGCTGCCTGCGGTCAGCTGCGCTCTAATAC
CATGAAAAAGGACCGGCAAAAAGCGGCAGCTGCAAGGACATGA

Upstream 100 bases:

>100_bases
AGCTCCTAATAAGACCAAGGGAAACCAAAGGCGCTCTCGTACTAGTCAACTGAACCATAAAACGGATCACTTTATCATCA
GAAAGAAGGACAATTAATCT

Downstream 100 bases:

>100_bases
TGGCTTGATCAAGGCTGCTGATATGATTGATAGGAGACTGGGATAAAAATCTGACCCTAAATAAAGCAAACAAACAGGTC
TAGTTTTCTGAGATCCCGAA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKPSIYGLTRDELIAWAIDNGQKAFRATQIWDWLYRKRIQSFDEMTNISKEFLAILKDSFCINPLKQRVAQESADGTVKY
LFELPDGMLIETVLMRQHYGQSVCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMMVQNYFDQRGQDERVSHVVVM
GIGEPFDNYQNVMTFLRTINDDHGLAIGARHITVSTSGLAHKIREFANEGVQVNLAVSLHAPNNELRSSIMRINRSFPLD
KLFSAIEYYIETTNRRVTFEYIMLNKVNDGVEQAQELADLTKRIRKLSYVNLIPYNPVSEHDQYSRSPKERVAAFYDILK
KNGVNCVVRQEHGTDIDAACGQLRSNTMKKDRQKAAAART

Sequences:

>Translated_360_residues
MKPSIYGLTRDELIAWAIDNGQKAFRATQIWDWLYRKRIQSFDEMTNISKEFLAILKDSFCINPLKQRVAQESADGTVKY
LFELPDGMLIETVLMRQHYGQSVCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMMVQNYFDQRGQDERVSHVVVM
GIGEPFDNYQNVMTFLRTINDDHGLAIGARHITVSTSGLAHKIREFANEGVQVNLAVSLHAPNNELRSSIMRINRSFPLD
KLFSAIEYYIETTNRRVTFEYIMLNKVNDGVEQAQELADLTKRIRKLSYVNLIPYNPVSEHDQYSRSPKERVAAFYDILK
KNGVNCVVRQEHGTDIDAACGQLRSNTMKKDRQKAAAART
>Mature_360_residues
MKPSIYGLTRDELIAWAIDNGQKAFRATQIWDWLYRKRIQSFDEMTNISKEFLAILKDSFCINPLKQRVAQESADGTVKY
LFELPDGMLIETVLMRQHYGQSVCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMMVQNYFDQRGQDERVSHVVVM
GIGEPFDNYQNVMTFLRTINDDHGLAIGARHITVSTSGLAHKIREFANEGVQVNLAVSLHAPNNELRSSIMRINRSFPLD
KLFSAIEYYIETTNRRVTFEYIMLNKVNDGVEQAQELADLTKRIRKLSYVNLIPYNPVSEHDQYSRSPKERVAAFYDILK
KNGVNCVVRQEHGTDIDAACGQLRSNTMKKDRQKAAAART

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=358, Percent_Identity=35.4748603351955, Blast_Score=222, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 40992; Mature: 40992

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSIYGLTRDELIAWAIDNGQKAFRATQIWDWLYRKRIQSFDEMTNISKEFLAILKDSF
CCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CINPLKQRVAQESADGTVKYLFELPDGMLIETVLMRQHYGQSVCVTTQVGCNIGCTFCAS
CCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
GLIKKQRDLNSGEITAQIMMVQNYFDQRGQDERVSHVVVMGIGEPFDNYQNVMTFLRTIN
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHCEEEEEECCCCCHHHHHHHHHHHHHC
DDHGLAIGARHITVSTSGLAHKIREFANEGVQVNLAVSLHAPNNELRSSIMRINRSFPLD
CCCCEEEEEEEEEEECHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCHH
KLFSAIEYYIETTNRRVTFEYIMLNKVNDGVEQAQELADLTKRIRKLSYVNLIPYNPVSE
HHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
HDQYSRSPKERVAAFYDILKKNGVNCVVRQEHGTDIDAACGQLRSNTMKKDRQKAAAART
CHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPSIYGLTRDELIAWAIDNGQKAFRATQIWDWLYRKRIQSFDEMTNISKEFLAILKDSF
CCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CINPLKQRVAQESADGTVKYLFELPDGMLIETVLMRQHYGQSVCVTTQVGCNIGCTFCAS
CCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
GLIKKQRDLNSGEITAQIMMVQNYFDQRGQDERVSHVVVMGIGEPFDNYQNVMTFLRTIN
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHCEEEEEECCCCCHHHHHHHHHHHHHC
DDHGLAIGARHITVSTSGLAHKIREFANEGVQVNLAVSLHAPNNELRSSIMRINRSFPLD
CCCCEEEEEEEEEEECHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCHH
KLFSAIEYYIETTNRRVTFEYIMLNKVNDGVEQAQELADLTKRIRKLSYVNLIPYNPVSE
HHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
HDQYSRSPKERVAAFYDILKKNGVNCVVRQEHGTDIDAACGQLRSNTMKKDRQKAAAART
CHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA