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The map label for this gene is isdE [H]

Identifier: 195977518

GI number: 195977518

Start: 399278

End: 400162

Strand: Direct

Name: isdE [H]

Synonym: Sez_0373

Alternate gene names: 195977518

Gene position: 399278-400162 (Clockwise)

Preceding gene: 195977517

Following gene: 195977519

Centisome position: 19.73

GC content: 41.47

Gene sequence:

>885_bases
ATGAAGAAAGTCATTAAGCTTATTGGATTAGCTGTTTTGTCCTTGGTGCTATCTGCTTGTGTGGATCAGCACCCTAAGGC
AGAGAAAAAGGCTGATAAGGAGAGAATTGTTGCCACCTCTGTGGCTGTTGTTGATATTTGTGATCGGCTAAACCTAGATT
TGATCGGGGTCTGTGACAGTAAGCTATACCAATTGCCAAAGCGTTACGATGCTGTCAAAAGGATCGGTCTACCGATGAAT
CCTGATATGGAGGTGATTGCTTCTCTAAAGCCAACGTGGATACTTAGTCCCAATTCTCTGCAAAATGATTTAGAGCCTAA
ATACCAGCAATTGGATACGGAATATGGCTTTTTGAATTTAACTAGCCTTGAAGGCATGTACCAGTCGATTGATGATTTGG
GGGACTTGTTTGATCGCAAAAAGGAAGCTAAGCGCCTACGGGCTGATTATGAGAAGTTTTACCGGGCCTTTAAGAAAAAG
CATAAAGGGCAAAAAAAGCCTAGGGTTCTTATTTTGATGGGGCTTCCTGGCAGCTATCTGATTGCTACAAATAAATCCTA
TGTTGGTAATCTGCTTGAGCTGGCTGGTGGTGAGAATGTTTATCACTCTGAGGACAAGGAATTTTTGTCTGCCAATCCAG
AGGACATGCTTCAGCAGCAGCCTGATCTGATTTTGCGTACTGCCCATGCCATTCCAGACAAGGTTAAGGACATGTTTGAT
AAGGAATTTGCTGAAAATGATATTTGGAAGCATTTTGAAGCTGTCAAATCTCAGCAGGTTTATGATTTGGATAATACCCT
TTTTGGCATGAGTGCTAAGTTTAACTACAAAGAGGCTTTGAATCATCTGTCACAAATCTTTTATGGAGCAAAGGAAGTCA
ACTAA

Upstream 100 bases:

>100_bases
CCAGTAAACAATCCATTGTCAGTTCTGGTTTACTATATACCAACAGCTCTTCTGCTGTCAAGAGCCTTGGTCTTGTTTAT
AAAGAAAAAGAGAAAAAACA

Downstream 100 bases:

>100_bases
GATGCAGCAAGGTAGTCAATATTCTGATCAAGTCACAGCAGCAAGAAAAAGAACCACTAGACTGGTTTATATGATTCTAT
TACTCATGCTGCTGTTTGGC

Product: ferrichrome ABC Transporter heme substrate-binding lipoprotein

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: Iron-regulated surface determinant protein E; Staphylococcal iron-regulated protein F [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKKVIKLIGLAVLSLVLSACVDQHPKAEKKADKERIVATSVAVVDICDRLNLDLIGVCDSKLYQLPKRYDAVKRIGLPMN
PDMEVIASLKPTWILSPNSLQNDLEPKYQQLDTEYGFLNLTSLEGMYQSIDDLGDLFDRKKEAKRLRADYEKFYRAFKKK
HKGQKKPRVLILMGLPGSYLIATNKSYVGNLLELAGGENVYHSEDKEFLSANPEDMLQQQPDLILRTAHAIPDKVKDMFD
KEFAENDIWKHFEAVKSQQVYDLDNTLFGMSAKFNYKEALNHLSQIFYGAKEVN

Sequences:

>Translated_294_residues
MKKVIKLIGLAVLSLVLSACVDQHPKAEKKADKERIVATSVAVVDICDRLNLDLIGVCDSKLYQLPKRYDAVKRIGLPMN
PDMEVIASLKPTWILSPNSLQNDLEPKYQQLDTEYGFLNLTSLEGMYQSIDDLGDLFDRKKEAKRLRADYEKFYRAFKKK
HKGQKKPRVLILMGLPGSYLIATNKSYVGNLLELAGGENVYHSEDKEFLSANPEDMLQQQPDLILRTAHAIPDKVKDMFD
KEFAENDIWKHFEAVKSQQVYDLDNTLFGMSAKFNYKEALNHLSQIFYGAKEVN
>Mature_294_residues
MKKVIKLIGLAVLSLVLSACVDQHPKAEKKADKERIVATSVAVVDICDRLNLDLIGVCDSKLYQLPKRYDAVKRIGLPMN
PDMEVIASLKPTWILSPNSLQNDLEPKYQQLDTEYGFLNLTSLEGMYQSIDDLGDLFDRKKEAKRLRADYEKFYRAFKKK
HKGQKKPRVLILMGLPGSYLIATNKSYVGNLLELAGGENVYHSEDKEFLSANPEDMLQQQPDLILRTAHAIPDKVKDMFD
KEFAENDIWKHFEAVKSQQVYDLDNTLFGMSAKFNYKEALNHLSQIFYGAKEVN

Specific function: Involved in heme (porphyrin) scavenging. Binds Fe(2+) and Fe(3+) heme but the largest fraction is Fe(2+) heme. Functions as a high-affinity heme binding protein and probably has a role in relaying heme-iron from cell wall-anchored isd proteins receptors t

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019957
- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 33552; Mature: 33552

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVIKLIGLAVLSLVLSACVDQHPKAEKKADKERIVATSVAVVDICDRLNLDLIGVCDS
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCH
KLYQLPKRYDAVKRIGLPMNPDMEVIASLKPTWILSPNSLQNDLEPKYQQLDTEYGFLNL
HHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEECCCHHHHHCCHHHHHHCCCCCEEEH
TSLEGMYQSIDDLGDLFDRKKEAKRLRADYEKFYRAFKKKHKGQKKPRVLILMGLPGSYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEE
IATNKSYVGNLLELAGGENVYHSEDKEFLSANPEDMLQQQPDLILRTAHAIPDKVKDMFD
EEECHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHCCCHHHHHHHHHCHHHHHHHHH
KEFAENDIWKHFEAVKSQQVYDLDNTLFGMSAKFNYKEALNHLSQIFYGAKEVN
HHHHHHHHHHHHHHHHHCCHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKVIKLIGLAVLSLVLSACVDQHPKAEKKADKERIVATSVAVVDICDRLNLDLIGVCDS
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCH
KLYQLPKRYDAVKRIGLPMNPDMEVIASLKPTWILSPNSLQNDLEPKYQQLDTEYGFLNL
HHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEECCCHHHHHCCHHHHHHCCCCCEEEH
TSLEGMYQSIDDLGDLFDRKKEAKRLRADYEKFYRAFKKKHKGQKKPRVLILMGLPGSYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEE
IATNKSYVGNLLELAGGENVYHSEDKEFLSANPEDMLQQQPDLILRTAHAIPDKVKDMFD
EEECHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHCCCHHHHHHHHHCHHHHHHHHH
KEFAENDIWKHFEAVKSQQVYDLDNTLFGMSAKFNYKEALNHLSQIFYGAKEVN
HHHHHHHHHHHHHHHHHCCHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA