Click here to switch to the map view.

The map label for this gene is insK [H]

Identifier: 195977491

GI number: 195977491

Start: 365364

End: 366167

Strand: Direct

Name: insK [H]

Synonym: Sez_0346

Alternate gene names: 195977491

Gene position: 365364-366167 (Clockwise)

Preceding gene: 195977490

Following gene: 195977494

Centisome position: 18.05

GC content: 40.67

Gene sequence:

>804_bases
TTGCTTGAAATCCTTGATTTATCGCGGTCAACCTATTATTATCAAGTCAAGCAACTAGCTCAAGAAGATAAGGACATGGA
CTTAAAGGAGCTCATTCAAGGCATCTATGATGAACATCATGGCAATTATGGCTATCGTCGCATTCATCTGGAACTAAGAA
ATCGTGGTTTTATCGTCAATCACAAAAAAGTACAACGTTTGATGACTGTCATGGGCTTAAAAGCTCGTATCCGTCGTAAG
CGCAAGTATTCTTCTTACAAAGGTGAGGTTGGCAAAAAGGCTGATAATCTGATTAAACGTCAGTTTGAAGGTTCTAAGCC
CTACGAGAAGTGCTATACCGATGTGACGGAATTTACCTTACCTGAGGGGAAACTCTATCTATCGCCTGTTCTTGACGGCT
ATAACAGTGAGATTATTGATTTCACCCTGTCTCGATCGCCTGACTTGAAGCAAGTACAAACCATGCTTGAGAAGGCTTTT
CCAGCGGATTCGTACAATGGAACGATTCTCCACAGCGATCAAGGCTGGCAATATCAACATCAGTCTTATCATCACTTTTT
GGAGACTAAAGGCATTCGTCCATCCATGTCTCGCAAGGGAAATAGTCCAGATAATGGGATGATGGAGTCCTTCTTTGGTA
TTCTCAAATCTGAGATGTTTTACGGCCTTGAGACAACTTATCAATCCCTTAATGAGCTTGAACAAGCTATCACAGATTAC
ATTTTTTACTACAACAACAAACGCATTAAAGCAAAGCTAAAAGGACTTAGCCCTGTGCAATACAGAACTAAATCCTTTCA
CTAA

Upstream 100 bases:

>100_bases
TGCTAAAAAAGCTGAGAGAATACCGGTTGAGGGACGAAGCAAAGCTCAAAGAGCAACAGAAATCATCCAAGAATTAATCG
GTCAATTTTCTCTAGCAACT

Downstream 100 bases:

>100_bases
TATGTCGTGTCCAACTTTTGGGGGGCAGTACAACGGATTGAGGAGTGTCGATTGTAGATCAGTGTCTGGTGTTGCAGCAA
TATGGTATAAAAAGGCTAGC

Product: transposase IS861 orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MLEILDLSRSTYYYQVKQLAQEDKDMDLKELIQGIYDEHHGNYGYRRIHLELRNRGFIVNHKKVQRLMTVMGLKARIRRK
RKYSSYKGEVGKKADNLIKRQFEGSKPYEKCYTDVTEFTLPEGKLYLSPVLDGYNSEIIDFTLSRSPDLKQVQTMLEKAF
PADSYNGTILHSDQGWQYQHQSYHHFLETKGIRPSMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLNELEQAITDY
IFYYNNKRIKAKLKGLSPVQYRTKSFH

Sequences:

>Translated_267_residues
MLEILDLSRSTYYYQVKQLAQEDKDMDLKELIQGIYDEHHGNYGYRRIHLELRNRGFIVNHKKVQRLMTVMGLKARIRRK
RKYSSYKGEVGKKADNLIKRQFEGSKPYEKCYTDVTEFTLPEGKLYLSPVLDGYNSEIIDFTLSRSPDLKQVQTMLEKAF
PADSYNGTILHSDQGWQYQHQSYHHFLETKGIRPSMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLNELEQAITDY
IFYYNNKRIKAKLKGLSPVQYRTKSFH
>Mature_267_residues
MLEILDLSRSTYYYQVKQLAQEDKDMDLKELIQGIYDEHHGNYGYRRIHLELRNRGFIVNHKKVQRLMTVMGLKARIRRK
RKYSSYKGEVGKKADNLIKRQFEGSKPYEKCYTDVTEFTLPEGKLYLSPVLDGYNSEIIDFTLSRSPDLKQVQTMLEKAF
PADSYNGTILHSDQGWQYQHQSYHHFLETKGIRPSMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLNELEQAITDY
IFYYNNKRIKAKLKGLSPVQYRTKSFH

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=254, Percent_Identity=29.1338582677165, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1789981, Length=267, Percent_Identity=48.689138576779, Blast_Score=262, Evalue=2e-71,
Organism=Escherichia coli, GI1788405, Length=272, Percent_Identity=28.3088235294118, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787263, Length=272, Percent_Identity=28.3088235294118, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1786753, Length=272, Percent_Identity=28.3088235294118, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1786570, Length=272, Percent_Identity=28.3088235294118, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1786490, Length=272, Percent_Identity=28.3088235294118, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31473; Mature: 31473

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEILDLSRSTYYYQVKQLAQEDKDMDLKELIQGIYDEHHGNYGYRRIHLELRNRGFIVN
CCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEC
HKKVQRLMTVMGLKARIRRKRKYSSYKGEVGKKADNLIKRQFEGSKPYEKCYTDVTEFTL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
PEGKLYLSPVLDGYNSEIIDFTLSRSPDLKQVQTMLEKAFPADSYNGTILHSDQGWQYQH
CCCCEEEEHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHH
QSYHHFLETKGIRPSMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLNELEQAITDY
HHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFYYNNKRIKAKLKGLSPVQYRTKSFH
HHHCCCCEEEEEECCCCCHHHHHCCCC
>Mature Secondary Structure
MLEILDLSRSTYYYQVKQLAQEDKDMDLKELIQGIYDEHHGNYGYRRIHLELRNRGFIVN
CCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEC
HKKVQRLMTVMGLKARIRRKRKYSSYKGEVGKKADNLIKRQFEGSKPYEKCYTDVTEFTL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
PEGKLYLSPVLDGYNSEIIDFTLSRSPDLKQVQTMLEKAFPADSYNGTILHSDQGWQYQH
CCCCEEEEHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHH
QSYHHFLETKGIRPSMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLNELEQAITDY
HHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFYYNNKRIKAKLKGLSPVQYRTKSFH
HHHCCCCEEEEEECCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]