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The map label for this gene is ksgA [H]

Identifier: 195977405

GI number: 195977405

Start: 279168

End: 280040

Strand: Direct

Name: ksgA [H]

Synonym: Sez_0258

Alternate gene names: 195977405

Gene position: 279168-280040 (Clockwise)

Preceding gene: 195977403

Following gene: 195977406

Centisome position: 13.79

GC content: 44.56

Gene sequence:

>873_bases
ATGAGAATTGCAGATTACAGTGTGACCAAGGCTATCCTTGATCGCTATGGCTTTACCTTTAAAAAATCATTTGGTCAAAA
TTTCTTGACGGATACTAATATTTTACAGAAGATTGTTGATACGGCTGAGATTGACAAGTCAGTCAACGTCATTGAGATTG
GTCCTGGTATCGGGGCTTTGACCGAATTTTTGGCAGAAAGGGCTGCAGAGGTCATGGCCTTTGAGATTGATGAGCGCTTG
GTGCCAATACTGGCTGACACGCTTCGTGATTTTGACAATGTTCAGGTGGTTAATCAGGACATTTTAAAGGCAGATTTACA
GACCCAGCTAAAGCAATTTAGCAATCCGGACCTTCCTATCAAGGTGGTAGCCAATTTGCCCTATTACATCACTACACCCA
TTTTGATGCACCTGATTGAAAGTAAGATTCCCTTCCAAGAATTTGTCGTCATGATGCAGCGCGAGGTGGCAGATCGGATT
TCAGCTGAGCCCAATACCAAGGCCTATGGCTCTTTGTCTATAGCAGTCCAGTATTATATGACCGCTAAGATAGCCTTTGT
TGTTCCTAGGACTGTTTTTGTGCCAGCACCAAATGTGGATTCGGCAATTCTGAAGATGACTCGTCGTGACCAGCCCTTGA
TTGAGGTTCAAGATGAGGATTTCTTTTTTAGGGTGTCACGAGTAGGCTTTGTGCATCGCAGAAAGACGCTTTGGAATAAC
TTGGTTAGCCATTTTGGCAAGGCTGAGGACACAAAGGCAAGGCTTGAGCAGGGACTAGCTCTAGCTGGAATCAAGCCTTC
TATCCGAGGAGAAGCGCTGTCTATTCAAGATTTTGGACGGTTAGCTGATGCCCTAAAGCAAGTAGGCTTATAG

Upstream 100 bases:

>100_bases
AAACGCTGTCAACGCGTTTTTTGTCTCTTTTTTTGCTGCGGCTCTGATAAACAATAGAAATATGCTACAATAGATATAAA
ACAATGAATAGGACATCAAC

Downstream 100 bases:

>100_bases
TGCCTTGGTTATAATTGTAGCTTGTCACAGCAATCCTGCTGTGGCTTTTTTAGATAGTGGTAGGCTGATCTTATCTTTGG
TGAAATTTGGGCTAAACTGC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRIADYSVTKAILDRYGFTFKKSFGQNFLTDTNILQKIVDTAEIDKSVNVIEIGPGIGALTEFLAERAAEVMAFEIDERL
VPILADTLRDFDNVQVVNQDILKADLQTQLKQFSNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
SAEPNTKAYGSLSIAVQYYMTAKIAFVVPRTVFVPAPNVDSAILKMTRRDQPLIEVQDEDFFFRVSRVGFVHRRKTLWNN
LVSHFGKAEDTKARLEQGLALAGIKPSIRGEALSIQDFGRLADALKQVGL

Sequences:

>Translated_290_residues
MRIADYSVTKAILDRYGFTFKKSFGQNFLTDTNILQKIVDTAEIDKSVNVIEIGPGIGALTEFLAERAAEVMAFEIDERL
VPILADTLRDFDNVQVVNQDILKADLQTQLKQFSNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
SAEPNTKAYGSLSIAVQYYMTAKIAFVVPRTVFVPAPNVDSAILKMTRRDQPLIEVQDEDFFFRVSRVGFVHRRKTLWNN
LVSHFGKAEDTKARLEQGLALAGIKPSIRGEALSIQDFGRLADALKQVGL
>Mature_290_residues
MRIADYSVTKAILDRYGFTFKKSFGQNFLTDTNILQKIVDTAEIDKSVNVIEIGPGIGALTEFLAERAAEVMAFEIDERL
VPILADTLRDFDNVQVVNQDILKADLQTQLKQFSNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
SAEPNTKAYGSLSIAVQYYMTAKIAFVVPRTVFVPAPNVDSAILKMTRRDQPLIEVQDEDFFFRVSRVGFVHRRKTLWNN
LVSHFGKAEDTKARLEQGLALAGIKPSIRGEALSIQDFGRLADALKQVGL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=289, Percent_Identity=30.7958477508651, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI7657198, Length=225, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1786236, Length=274, Percent_Identity=32.1167883211679, Blast_Score=145, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI25146882, Length=226, Percent_Identity=31.4159292035398, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25141369, Length=310, Percent_Identity=26.1290322580645, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=223, Percent_Identity=30.0448430493274, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21358017, Length=226, Percent_Identity=30.9734513274336, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21357273, Length=301, Percent_Identity=25.9136212624585, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 32655; Mature: 32655

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIADYSVTKAILDRYGFTFKKSFGQNFLTDTNILQKIVDTAEIDKSVNVIEIGPGIGAL
CCCCHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHH
TEFLAERAAEVMAFEIDERLVPILADTLRDFDNVQVVNQDILKADLQTQLKQFSNPDLPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCE
KVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRISAEPNTKAYGSLSIAVQYYM
EEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
TAKIAFVVPRTVFVPAPNVDSAILKMTRRDQPLIEVQDEDFFFRVSRVGFVHRRKTLWNN
HHHHHEEECCEEEECCCCCCHHHHHHHHCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHH
LVSHFGKAEDTKARLEQGLALAGIKPSIRGEALSIQDFGRLADALKQVGL
HHHHCCCCCHHHHHHHCCCEEECCCCCCCCCEECHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIADYSVTKAILDRYGFTFKKSFGQNFLTDTNILQKIVDTAEIDKSVNVIEIGPGIGAL
CCCCHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHH
TEFLAERAAEVMAFEIDERLVPILADTLRDFDNVQVVNQDILKADLQTQLKQFSNPDLPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCE
KVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRISAEPNTKAYGSLSIAVQYYM
EEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
TAKIAFVVPRTVFVPAPNVDSAILKMTRRDQPLIEVQDEDFFFRVSRVGFVHRRKTLWNN
HHHHHEEECCEEEECCCCCCHHHHHHHHCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHH
LVSHFGKAEDTKARLEQGLALAGIKPSIRGEALSIQDFGRLADALKQVGL
HHHHCCCCCHHHHHHHCCCEEECCCCCCCCCEECHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA