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The map label for this gene is gltX [H]

Identifier: 195977374

GI number: 195977374

Start: 250356

End: 251801

Strand: Direct

Name: gltX [H]

Synonym: Sez_0227

Alternate gene names: 195977374

Gene position: 250356-251801 (Clockwise)

Preceding gene: 195977373

Following gene: 195977375

Centisome position: 12.37

GC content: 45.23

Gene sequence:

>1446_bases
ATGTCTAAACCAATTCGTGTGCGCTACGCACCAAGCCCAACAGGTCTTTTACATATCGGAAATGCGCGGACAGCGCTCTT
TAACTATCTTTATGCTCGTCATCATGGCGGTGACTTTGTTATTCGTATTGAGGATACTGATCGTAAGCGCCATGTCGAGG
ACGGTGAGCGTTCTCAGCTGGAAAATTTGAGATGGCTTGGTATAGATTGGGACGAAAGCCCTGAGACGCATGAGCGTTAC
CGTCAGTCAGAGCGTCTAGCTCTTTATCAACAGTACATTGACCAATTATTAGCAGAAGGCAAGGCCTACAAGTCTTATGT
GACTGAGGAGGAGTTGGCAGCAGAGCGTGAGCGTCAAGAGGCAGCTGGTGAAACACCTCGCTATGTCAATGAATATCTTG
GCATGTCAGCAGATGAAAAGGCGGCCTATATTGCAGAGCGTGAAGCAGCAGGTATTGTGCCTACGGTTCGTTTGGCTGTC
AATGAGGCTGGTATTTACAAATGGACAGACATGGTAAAAGGCGATATCGTATTTGAGGGTGGCAATGTCGGTGGTGACTG
GGTAATCCAGAAAAAAGACGGCTATCCAACCTACAATTTTGCTGTTGTTATTGATGATCACGATATGCAAATCTCACACG
TTATCCGCGGAGATGACCATATTGCCAATACGCCAAAGCAGTTGATGGTTTACGAGGCGCTTGGCTGGGAAGCACCCGTC
TTTGGTCATATGACCCTTATCATTAATTCTGAGACAGGTAAAAAGCTATCAAAGCGTGATACCAATACCCTGCAGTTTAT
TGAGGATTACCGCAAAAAAGGCTATATGCCAGAGGCAGTCTTTAATTTTATTGCCCTTTTAGGCTGGAATCCTGGCGGGG
AGGCTGAGATCTTTTCTCGTGAGCAATTGATTGCCTTATTTGATGAGAGTCGTCTTAGCAAGTCGCCAGCAGCCTTTGAT
CAGAAAAAAATGGACTGGATGAGCAATGAGTACTTTAAGCATGCTGATCTTGGCGCTGTATATGCGCTTTGTAAGCCATT
TCTTGAAGCAGCAGGCCGCTTGACTGAAAAAGCAGAGAAGCTAGTTGAGCTCTATCAGCCTCAATTAAGCTCTGCTGATG
AGATTGTTCCATTGACTGATTTATTTTTCTCAGATTTCCCAGAGCTGACCGCAAAAGAAAAAGAAGTGATGGCAGCTGAG
ACTGTTCCAACAGTTCTGAAGGCCTTCAAGGAAAAGCTAGAGGCGATGTCAGATGAGGACTTTAAGCCAGAAAACATTTT
CCCACAAATCAAGGCTGTCCAAAAAGAAACCGGCATTAAGGGCAAGAACCTCTTTATGCCAATTCGTATTGCTGTTTCTG
GAGAAATGCATGGCCCAGAGCTGCCAAATACCATTTACCTCCTTGGACGTGAACAATCGATTGAGCATATTGCCAATATG
CTTTAA

Upstream 100 bases:

>100_bases
TTTTGTTCCTTATCGACCATTTTCTCATAGAGCAAGCCTACCGTAGGAACGGTTTATAGCCCAGGATCCCTTCTTTCAAT
GAAACCATAGGAGAAAAAGA

Downstream 100 bases:

>100_bases
ACACTATCGCAGCTAGTAGTTTGGTCTAGGCTGACGATGATCATAAATAACATCATGAATGAAGCAGAGATGAGATATCT
GATGTTTGCTTCATTCTTTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIEDTDRKRHVEDGERSQLENLRWLGIDWDESPETHERY
RQSERLALYQQYIDQLLAEGKAYKSYVTEEELAAERERQEAAGETPRYVNEYLGMSADEKAAYIAEREAAGIVPTVRLAV
NEAGIYKWTDMVKGDIVFEGGNVGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPV
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMPEAVFNFIALLGWNPGGEAEIFSREQLIALFDESRLSKSPAAFD
QKKMDWMSNEYFKHADLGAVYALCKPFLEAAGRLTEKAEKLVELYQPQLSSADEIVPLTDLFFSDFPELTAKEKEVMAAE
TVPTVLKAFKEKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPNTIYLLGREQSIEHIANM
L

Sequences:

>Translated_481_residues
MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIEDTDRKRHVEDGERSQLENLRWLGIDWDESPETHERY
RQSERLALYQQYIDQLLAEGKAYKSYVTEEELAAERERQEAAGETPRYVNEYLGMSADEKAAYIAEREAAGIVPTVRLAV
NEAGIYKWTDMVKGDIVFEGGNVGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPV
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMPEAVFNFIALLGWNPGGEAEIFSREQLIALFDESRLSKSPAAFD
QKKMDWMSNEYFKHADLGAVYALCKPFLEAAGRLTEKAEKLVELYQPQLSSADEIVPLTDLFFSDFPELTAKEKEVMAAE
TVPTVLKAFKEKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPNTIYLLGREQSIEHIANM
L
>Mature_480_residues
SKPIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIEDTDRKRHVEDGERSQLENLRWLGIDWDESPETHERYR
QSERLALYQQYIDQLLAEGKAYKSYVTEEELAAERERQEAAGETPRYVNEYLGMSADEKAAYIAEREAAGIVPTVRLAVN
EAGIYKWTDMVKGDIVFEGGNVGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPVF
GHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMPEAVFNFIALLGWNPGGEAEIFSREQLIALFDESRLSKSPAAFDQ
KKMDWMSNEYFKHADLGAVYALCKPFLEAAGRLTEKAEKLVELYQPQLSSADEIVPLTDLFFSDFPELTAKEKEVMAAET
VPTVLKAFKEKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPNTIYLLGREQSIEHIANML

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=498, Percent_Identity=30.5220883534137, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI62241042, Length=258, Percent_Identity=26.7441860465116, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788743, Length=480, Percent_Identity=37.5, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI226510925, Length=253, Percent_Identity=30.4347826086957, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71993394, Length=499, Percent_Identity=31.6633266533066, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17543332, Length=298, Percent_Identity=24.8322147651007, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=341, Percent_Identity=39.2961876832845, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6321192, Length=218, Percent_Identity=29.8165137614679, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324742, Length=313, Percent_Identity=24.2811501597444, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24665321, Length=501, Percent_Identity=30.938123752495, Blast_Score=235, Evalue=5e-62,
Organism=Drosophila melanogaster, GI17864488, Length=300, Percent_Identity=26.6666666666667, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 54734; Mature: 54602

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIEDTDRKRHVEDGERSQL
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHH
ENLRWLGIDWDESPETHERYRQSERLALYQQYIDQLLAEGKAYKSYVTEEELAAERERQE
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHH
AAGETPRYVNEYLGMSADEKAAYIAEREAAGIVPTVRLAVNEAGIYKWTDMVKGDIVFEG
HCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEHHHCCCCCEEEEC
GNVGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPV
CCCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHCCCHHHHEEEHHCCCCCCC
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMPEAVFNFIALLGWNPGGEAEIFSR
EEEEEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCH
EQLIALFDESRLSKSPAAFDQKKMDWMSNEYFKHADLGAVYALCKPFLEAAGRLTEKAEK
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LVELYQPQLSSADEIVPLTDLFFSDFPELTAKEKEVMAAETVPTVLKAFKEKLEAMSDED
HHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FKPENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPNTIYLLGREQSIEHIANM
CCCCCCCHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEEECCHHHHHHHHC
L
C
>Mature Secondary Structure 
SKPIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIEDTDRKRHVEDGERSQL
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHH
ENLRWLGIDWDESPETHERYRQSERLALYQQYIDQLLAEGKAYKSYVTEEELAAERERQE
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHH
AAGETPRYVNEYLGMSADEKAAYIAEREAAGIVPTVRLAVNEAGIYKWTDMVKGDIVFEG
HCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEHHHCCCCCEEEEC
GNVGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPV
CCCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHCCCHHHHEEEHHCCCCCCC
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMPEAVFNFIALLGWNPGGEAEIFSR
EEEEEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCH
EQLIALFDESRLSKSPAAFDQKKMDWMSNEYFKHADLGAVYALCKPFLEAAGRLTEKAEK
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LVELYQPQLSSADEIVPLTDLFFSDFPELTAKEKEVMAAETVPTVLKAFKEKLEAMSDED
HHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FKPENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPNTIYLLGREQSIEHIANM
CCCCCCCHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEEECCHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA