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The map label for this gene is tilS [H]

Identifier: 195977168

GI number: 195977168

Start: 12627

End: 13910

Strand: Direct

Name: tilS [H]

Synonym: Sez_0012

Alternate gene names: 195977168

Gene position: 12627-13910 (Clockwise)

Preceding gene: 195977167

Following gene: 195977169

Centisome position: 0.62

GC content: 37.77

Gene sequence:

>1284_bases
ATGACTTATCAACACATTTATAACATCATAAAAAAGAAGGCCTATTTTGACGCTCATCAGGCTGTTTTGATAGCTGTATC
TGGTGGAGTAGATTCGATGAATTTGCTGCACTTTTTGCATGCTTTTCAAGCAGAATTGCAAATTCGGATTGGCATTGCGC
ATGTCAATCACAAGCAAAGACCAGAATCAGATGACGAAGAGGCTTATTTGAGGTCTTGGGCTAAAAAGCATGCTATTCCT
ATCTATGTTGCTTATTTTCAGGGCGCTTTTTCTGAGAATGCTGCGCGTCATTTTCGCTATCAATTTTTTGAAGAAATCAT
GCAGCAGGAGCATTATTCGGCTTTAGTCACAGCACATCATGCTGATGATCAGGCTGAAACTATTTTGATGAGGCTGATTC
GTGGCAGTCGATTGCGTCATTTAGCTGGTATCAGAGAGGTTCAGCCCTTTGCTACTGGTCAGTTGATACGACCGTTTTTA
ACAGTATCAAAAGAAGAGTTGCCTAATCCCTTTCATTTTGAAGATCATTCTAATGACAGCATGGCTTATTTTCGCAATCG
TGTTCGTCATCATTACCTGCCAGATTTCAAAAGAGAAAATCCTCAGGCTACTCAATCTTTGATTGCTTTATCTGCGGAAA
GTCGCCTGCTGCTTCAGGCCTTTGATGATTTAACAAAAGGACTTGCATTTCACCGATTAGATTGTTTTTTAGCTCAATCA
GCTGCTGTTCAGTTTTTTTTGCTTCAGCATTATTTAGAAACGTTTCCGCAGCTAGCTATTAAAAAATCACAATTTGATGA
TCTTTTACACATCATCAGACGGCAAAAGCAGGGGATTTATCCAATTAAAAATACTTATTGTCTCCTCATTGAAAAAGAGT
CTTTTGTTATTAAAAAAATCATTCCAAAGACTGATTTAAATCGAGAATCAAAAATGGTATCATACGGTGATCCTTTAGAT
TATGGAGGGTATCGATTTGCTTTTTCAGGTGACTTGACTGACAAAGGACATGATATAGTTATTCCTTTATATAGCTTATC
ACCAGTTACCTTGCGTCACAGGCAGGCAGGAGATCGGCTTTTTCTTGGTGAATTTTCAAAAAAATTAAGACGATTGTTTA
TCGATGGAAAGTTTACAAACGAGCAGCGTCAAAATGCCATCGTAGGAGAACAGGCCGGAGTTATTATTTTTGTGCTGGTT
GGCGATGAAACATATTTGAGAAAAGCGTCTAAACATGATATAATGTTGGCTAAACTGTATATTGATAAATTAGAAAAAAG
GTGA

Upstream 100 bases:

>100_bases
TTGTTTATGGTGATAATCCCTTTATCTTATCCGTTTTTACAGATAAGGCTTCCTATGACGACATTACAGCAATTGCTGAC
GATGTTTATTCGATTTTAAA

Downstream 100 bases:

>100_bases
CAGTATGCTTGAGCAAGATATTCAAAAAATTCTTTATTCTGAAAATGACATTATTCAAAGGACCAAGGAGCTTGGTGAGC
AGTTGACAAAGGATTATCAT

Product: tRNA(Ile)-lysidine synthase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTYQHIYNIIKKKAYFDAHQAVLIAVSGGVDSMNLLHFLHAFQAELQIRIGIAHVNHKQRPESDDEEAYLRSWAKKHAIP
IYVAYFQGAFSENAARHFRYQFFEEIMQQEHYSALVTAHHADDQAETILMRLIRGSRLRHLAGIREVQPFATGQLIRPFL
TVSKEELPNPFHFEDHSNDSMAYFRNRVRHHYLPDFKRENPQATQSLIALSAESRLLLQAFDDLTKGLAFHRLDCFLAQS
AAVQFFLLQHYLETFPQLAIKKSQFDDLLHIIRRQKQGIYPIKNTYCLLIEKESFVIKKIIPKTDLNRESKMVSYGDPLD
YGGYRFAFSGDLTDKGHDIVIPLYSLSPVTLRHRQAGDRLFLGEFSKKLRRLFIDGKFTNEQRQNAIVGEQAGVIIFVLV
GDETYLRKASKHDIMLAKLYIDKLEKR

Sequences:

>Translated_427_residues
MTYQHIYNIIKKKAYFDAHQAVLIAVSGGVDSMNLLHFLHAFQAELQIRIGIAHVNHKQRPESDDEEAYLRSWAKKHAIP
IYVAYFQGAFSENAARHFRYQFFEEIMQQEHYSALVTAHHADDQAETILMRLIRGSRLRHLAGIREVQPFATGQLIRPFL
TVSKEELPNPFHFEDHSNDSMAYFRNRVRHHYLPDFKRENPQATQSLIALSAESRLLLQAFDDLTKGLAFHRLDCFLAQS
AAVQFFLLQHYLETFPQLAIKKSQFDDLLHIIRRQKQGIYPIKNTYCLLIEKESFVIKKIIPKTDLNRESKMVSYGDPLD
YGGYRFAFSGDLTDKGHDIVIPLYSLSPVTLRHRQAGDRLFLGEFSKKLRRLFIDGKFTNEQRQNAIVGEQAGVIIFVLV
GDETYLRKASKHDIMLAKLYIDKLEKR
>Mature_426_residues
TYQHIYNIIKKKAYFDAHQAVLIAVSGGVDSMNLLHFLHAFQAELQIRIGIAHVNHKQRPESDDEEAYLRSWAKKHAIPI
YVAYFQGAFSENAARHFRYQFFEEIMQQEHYSALVTAHHADDQAETILMRLIRGSRLRHLAGIREVQPFATGQLIRPFLT
VSKEELPNPFHFEDHSNDSMAYFRNRVRHHYLPDFKRENPQATQSLIALSAESRLLLQAFDDLTKGLAFHRLDCFLAQSA
AVQFFLLQHYLETFPQLAIKKSQFDDLLHIIRRQKQGIYPIKNTYCLLIEKESFVIKKIIPKTDLNRESKMVSYGDPLDY
GGYRFAFSGDLTDKGHDIVIPLYSLSPVTLRHRQAGDRLFLGEFSKKLRRLFIDGKFTNEQRQNAIVGEQAGVIIFVLVG
DETYLRKASKHDIMLAKLYIDKLEKR

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=209, Percent_Identity=34.4497607655502, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 49584; Mature: 49452

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYQHIYNIIKKKAYFDAHQAVLIAVSGGVDSMNLLHFLHAFQAELQIRIGIAHVNHKQR
CCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHEEEEEEEEECCCCCC
PESDDEEAYLRSWAKKHAIPIYVAYFQGAFSENAARHFRYQFFEEIMQQEHYSALVTAHH
CCCCCHHHHHHHHHHHCCCEEEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
ADDQAETILMRLIRGSRLRHLAGIREVQPFATGQLIRPFLTVSKEELPNPFHFEDHSNDS
CCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
MAYFRNRVRHHYLPDFKRENPQATQSLIALSAESRLLLQAFDDLTKGLAFHRLDCFLAQS
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVQFFLLQHYLETFPQLAIKKSQFDDLLHIIRRQKQGIYPIKNTYCLLIEKESFVIKKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHH
IPKTDLNRESKMVSYGDPLDYGGYRFAFSGDLTDKGHDIVIPLYSLSPVTLRHRQAGDRL
CCCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCHHHHHCCCCEE
FLGEFSKKLRRLFIDGKFTNEQRQNAIVGEQAGVIIFVLVGDETYLRKASKHDIMLAKLY
EHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEEEEECCHHHHHHCCCCCEEHHHHH
IDKLEKR
HHHHHCC
>Mature Secondary Structure 
TYQHIYNIIKKKAYFDAHQAVLIAVSGGVDSMNLLHFLHAFQAELQIRIGIAHVNHKQR
CHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHEEEEEEEEECCCCCC
PESDDEEAYLRSWAKKHAIPIYVAYFQGAFSENAARHFRYQFFEEIMQQEHYSALVTAHH
CCCCCHHHHHHHHHHHCCCEEEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
ADDQAETILMRLIRGSRLRHLAGIREVQPFATGQLIRPFLTVSKEELPNPFHFEDHSNDS
CCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
MAYFRNRVRHHYLPDFKRENPQATQSLIALSAESRLLLQAFDDLTKGLAFHRLDCFLAQS
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVQFFLLQHYLETFPQLAIKKSQFDDLLHIIRRQKQGIYPIKNTYCLLIEKESFVIKKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHH
IPKTDLNRESKMVSYGDPLDYGGYRFAFSGDLTDKGHDIVIPLYSLSPVTLRHRQAGDRL
CCCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCHHHHHCCCCEE
FLGEFSKKLRRLFIDGKFTNEQRQNAIVGEQAGVIIFVLVGDETYLRKASKHDIMLAKLY
EHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEEEEECCHHHHHHCCCCCEEHHHHH
IDKLEKR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA