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The map label for this gene is fixK [H]

Identifier: 195953019

GI number: 195953019

Start: 578310

End: 579041

Strand: Direct

Name: fixK [H]

Synonym: HY04AAS1_0644

Alternate gene names: 195953019

Gene position: 578310-579041 (Clockwise)

Preceding gene: 195953018

Following gene: 195953020

Centisome position: 37.08

GC content: 34.15

Gene sequence:

>732_bases
ATGGAAACAATGGAAAAGCCTAAGAGCACCATAGATCAGAAAATAAAGTTTCTGAAAAGCGTTAGCATGTTTGAGAATAT
GTCTAACGACGAAATAAAAGAAGTAGCAAGTCTTCTAAATATGAGAGAATATAGAAAAAACGAATATATATTCTTTGAAG
AGGAATCTGAACCTGGACTTTTTATATTGATGGATGGTATAGTTAAGCTCATAAAAGAAACCCAGGACGGCCGTACAATT
ATAGTAAGGCTCGTATTTCCAAAAGATACCTTTGGATGGATTGAGTGGGGTGGGTCAAATATAAAGTCAAAAATAAAAAC
TACATACACAGCTCAATCTATGACAGAATCATCTGTTTTATACCTTTCAAACCAAGACTTTATAAATTTAGCTATCAAGT
ATCCAGCTTTTGCTGTAAAAGTAACTTGTGACGCTACCCATAATCTTTTATACGCTTACGATATATTAAAATCCATAGCT
TCTGGCAGAGTAGAAGAGCGCATAGCAAGAGTGATATTAGATCTGGCAAAGTCCGTAGGTGAAGAAAAAGAAGGTGGTAA
AATATCAATAGAAATACCTATAACCCGTCAAGATATAGCAGAAATGACAGGTACTACCGTAGAAACAGCCATACGTATTA
TGAGTAAATGGAAAAAAGAAGGCATAATACATACGGAAAGAGGCTATATAGAGATACTTAAGAAAAGAGAATTAGAAAGG
CTCATGATATAA

Upstream 100 bases:

>100_bases
AAAGAATATCTTGAAAAAATATTTTCTTTGCATAAATTTAATATATGATGGTATAATCATATGGTTATATCACTAATTTA
TATTATTTTTGGAGGTATGT

Downstream 100 bases:

>100_bases
GCTAAATATTATAAGGGTAAAACATTGGAAGGTCACGTACTAATAGTTAGTTTTGGGTCTCAATATGTACAGCTTATAGC
TAGAAAAGTAAGAGAATTAG

Product: Crp/Fnr family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
METMEKPKSTIDQKIKFLKSVSMFENMSNDEIKEVASLLNMREYRKNEYIFFEEESEPGLFILMDGIVKLIKETQDGRTI
IVRLVFPKDTFGWIEWGGSNIKSKIKTTYTAQSMTESSVLYLSNQDFINLAIKYPAFAVKVTCDATHNLLYAYDILKSIA
SGRVEERIARVILDLAKSVGEEKEGGKISIEIPITRQDIAEMTGTTVETAIRIMSKWKKEGIIHTERGYIEILKKRELER
LMI

Sequences:

>Translated_243_residues
METMEKPKSTIDQKIKFLKSVSMFENMSNDEIKEVASLLNMREYRKNEYIFFEEESEPGLFILMDGIVKLIKETQDGRTI
IVRLVFPKDTFGWIEWGGSNIKSKIKTTYTAQSMTESSVLYLSNQDFINLAIKYPAFAVKVTCDATHNLLYAYDILKSIA
SGRVEERIARVILDLAKSVGEEKEGGKISIEIPITRQDIAEMTGTTVETAIRIMSKWKKEGIIHTERGYIEILKKRELER
LMI
>Mature_243_residues
METMEKPKSTIDQKIKFLKSVSMFENMSNDEIKEVASLLNMREYRKNEYIFFEEESEPGLFILMDGIVKLIKETQDGRTI
IVRLVFPKDTFGWIEWGGSNIKSKIKTTYTAQSMTESSVLYLSNQDFINLAIKYPAFAVKVTCDATHNLLYAYDILKSIA
SGRVEERIARVILDLAKSVGEEKEGGKISIEIPITRQDIAEMTGTTVETAIRIMSKWKKEGIIHTERGYIEILKKRELER
LMI

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 27938; Mature: 27938

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METMEKPKSTIDQKIKFLKSVSMFENMSNDEIKEVASLLNMREYRKNEYIFFEEESEPGL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCE
FILMDGIVKLIKETQDGRTIIVRLVFPKDTFGWIEWGGSNIKSKIKTTYTAQSMTESSVL
EEEHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEE
YLSNQDFINLAIKYPAFAVKVTCDATHNLLYAYDILKSIASGRVEERIARVILDLAKSVG
EEECCCEEEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
EEKEGGKISIEIPITRQDIAEMTGTTVETAIRIMSKWKKEGIIHTERGYIEILKKRELER
CCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHH
LMI
HCC
>Mature Secondary Structure
METMEKPKSTIDQKIKFLKSVSMFENMSNDEIKEVASLLNMREYRKNEYIFFEEESEPGL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCE
FILMDGIVKLIKETQDGRTIIVRLVFPKDTFGWIEWGGSNIKSKIKTTYTAQSMTESSVL
EEEHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEE
YLSNQDFINLAIKYPAFAVKVTCDATHNLLYAYDILKSIASGRVEERIARVILDLAKSVG
EEECCCEEEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
EEKEGGKISIEIPITRQDIAEMTGTTVETAIRIMSKWKKEGIIHTERGYIEILKKRELER
CCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHH
LMI
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]