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The map label for this gene is glcD [H]

Identifier: 195952900

GI number: 195952900

Start: 462382

End: 463752

Strand: Direct

Name: glcD [H]

Synonym: HY04AAS1_0525

Alternate gene names: 195952900

Gene position: 462382-463752 (Clockwise)

Preceding gene: 195952899

Following gene: 195952913

Centisome position: 29.65

GC content: 35.23

Gene sequence:

>1371_bases
ATGGAAAAAAAATTAAAAAAACTTAAAGATGCTTTGGGCGATAAAATTAACGATTCTATAGTAGAAAGAAAACTCTACTC
TTACGATGCTACTGCCATTCCAATAGAACATACTACGCCTATAGCTGTTGCTTTTCCTACATCTTCAGAAGATATAGAAA
TGATAGTAAAAATTTGCTATGAAGAAGATATACCCATGGTACCAAGAGGGGCAGGATCAGGTTTGACAGGCGGTGCTACA
CCGGTAGTAGACGGTTCTTTGGTTATATCCCTTGAAAAAATGAATAAATTTCATGTAGACGTAGGCAATACCACAGCTTA
CGTGGAGCCAGGGGTTATTACATCGGAATTACAAGACTACGTGGAATCTCTAGGATTATTTTATCCACCAGATCCTTCTA
GCTTTAAATATTCTACCATAGGTGGCAACATAGCAGAAAATGCCGGTGGTCCAAGGTGTTTGAAGTATGGGGTCACAAGG
GAATATGTGCTAGGGCTAGAAATGTATATAAAAGAAGGAAAGCTGTTAAAAACTGGAAACCCCATTATAAAAGATGTAGC
AGGATATGATCTAACGAGGCTTGTAGTTGGTTCGGAAGGAACACTTGGTATAGTATCAAAAGCTATATTAAAATTGATAC
CAAAACCACCTTACAAATCAACCATAATGGGGATATTCAACAACATAGAAAATATAGGTAAATCTGTAACCAAAATATTA
ACGTCTGGTATATTACCTTGCGCATTGGAATTTATGGATAAAAATTCTATACAAGCTGTACAAAAATTCTATCCAGCCGG
TATTCCAGAAGATGCCGAAGGAGTCTTATTAATAGAGCTTGACGGACAAAAAGAAAGTGTAGAAAAAGAAAAAATGGAGG
TATCCAACATACTAAAGTCTTTAGGAGCAGGTGTTATACCAGCCCAAAACCCTCAACACGAACAAAAACTTTGGAGTGCA
AGAAAAAATATAGGTCCAGCCCTTGCTAACATCAAATCTGGTAAGATAAACGAGGATATAGTTTTCCCACGGATATATCT
ATCTGAAGCTCTGCAAAAAATAAACAAAATAGCAAAAAAATATAACCTTACTATAGTAAATTTTGGACATATAGGAGACG
GTAATCTCCATGTAAACATACTTTACGATAAATTAAATAAAGATGAATTAGAAAGAGCAGAAAAGGCAGTAGATGAAGTG
TTTGAGTTAGCTCTTAATTATCAGGGTTCTATCACTGGCGAACACGGTGTTGGTCTTACTAAAAAGAAATTTTTAGAATG
GCAGTTAAAAGACGTAGGTATTGAGCTTTTAAAAGCTATAAAACAAAGTTTTGATCCAAAAAATTTATTTAACCCAGGTA
AAGTTATTTAG

Upstream 100 bases:

>100_bases
ACCAGAACAGATAGCTTATATGTCCTCTTGGGAACATGGCACATAATCTCTAATGATTAAAACCCTTGACTTGCTTAAGA
TTTAAGTTATTATCTAATAC

Downstream 100 bases:

>100_bases
AAACTAATCTATGTAATAGCGCCATAAACGTATGACCTACCATATAAACCCACATTGAATTTCCAAAAAAATCATGAATT
TCCATAAAAAAATGAACGAT

Product: FAD linked oxidase domain-containing protein

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MEKKLKKLKDALGDKINDSIVERKLYSYDATAIPIEHTTPIAVAFPTSSEDIEMIVKICYEEDIPMVPRGAGSGLTGGAT
PVVDGSLVISLEKMNKFHVDVGNTTAYVEPGVITSELQDYVESLGLFYPPDPSSFKYSTIGGNIAENAGGPRCLKYGVTR
EYVLGLEMYIKEGKLLKTGNPIIKDVAGYDLTRLVVGSEGTLGIVSKAILKLIPKPPYKSTIMGIFNNIENIGKSVTKIL
TSGILPCALEFMDKNSIQAVQKFYPAGIPEDAEGVLLIELDGQKESVEKEKMEVSNILKSLGAGVIPAQNPQHEQKLWSA
RKNIGPALANIKSGKINEDIVFPRIYLSEALQKINKIAKKYNLTIVNFGHIGDGNLHVNILYDKLNKDELERAEKAVDEV
FELALNYQGSITGEHGVGLTKKKFLEWQLKDVGIELLKAIKQSFDPKNLFNPGKVI

Sequences:

>Translated_456_residues
MEKKLKKLKDALGDKINDSIVERKLYSYDATAIPIEHTTPIAVAFPTSSEDIEMIVKICYEEDIPMVPRGAGSGLTGGAT
PVVDGSLVISLEKMNKFHVDVGNTTAYVEPGVITSELQDYVESLGLFYPPDPSSFKYSTIGGNIAENAGGPRCLKYGVTR
EYVLGLEMYIKEGKLLKTGNPIIKDVAGYDLTRLVVGSEGTLGIVSKAILKLIPKPPYKSTIMGIFNNIENIGKSVTKIL
TSGILPCALEFMDKNSIQAVQKFYPAGIPEDAEGVLLIELDGQKESVEKEKMEVSNILKSLGAGVIPAQNPQHEQKLWSA
RKNIGPALANIKSGKINEDIVFPRIYLSEALQKINKIAKKYNLTIVNFGHIGDGNLHVNILYDKLNKDELERAEKAVDEV
FELALNYQGSITGEHGVGLTKKKFLEWQLKDVGIELLKAIKQSFDPKNLFNPGKVI
>Mature_456_residues
MEKKLKKLKDALGDKINDSIVERKLYSYDATAIPIEHTTPIAVAFPTSSEDIEMIVKICYEEDIPMVPRGAGSGLTGGAT
PVVDGSLVISLEKMNKFHVDVGNTTAYVEPGVITSELQDYVESLGLFYPPDPSSFKYSTIGGNIAENAGGPRCLKYGVTR
EYVLGLEMYIKEGKLLKTGNPIIKDVAGYDLTRLVVGSEGTLGIVSKAILKLIPKPPYKSTIMGIFNNIENIGKSVTKIL
TSGILPCALEFMDKNSIQAVQKFYPAGIPEDAEGVLLIELDGQKESVEKEKMEVSNILKSLGAGVIPAQNPQHEQKLWSA
RKNIGPALANIKSGKINEDIVFPRIYLSEALQKINKIAKKYNLTIVNFGHIGDGNLHVNILYDKLNKDELERAEKAVDEV
FELALNYQGSITGEHGVGLTKKKFLEWQLKDVGIELLKAIKQSFDPKNLFNPGKVI

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=451, Percent_Identity=29.490022172949, Blast_Score=226, Evalue=5e-59,
Organism=Homo sapiens, GI37595754, Length=474, Percent_Identity=28.0590717299578, Blast_Score=216, Evalue=4e-56,
Organism=Homo sapiens, GI119964728, Length=422, Percent_Identity=31.042654028436, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI4501993, Length=454, Percent_Identity=25.3303964757709, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1789351, Length=436, Percent_Identity=37.3853211009174, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI1787977, Length=519, Percent_Identity=23.8921001926782, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI48994907, Length=451, Percent_Identity=22.3946784922395, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI48994914, Length=113, Percent_Identity=38.0530973451327, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71992373, Length=469, Percent_Identity=29.637526652452, Blast_Score=214, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI71992381, Length=469, Percent_Identity=28.997867803838, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI71992392, Length=356, Percent_Identity=30.8988764044944, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17534361, Length=432, Percent_Identity=30.5555555555556, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17556096, Length=461, Percent_Identity=23.4273318872017, Blast_Score=111, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6320027, Length=476, Percent_Identity=28.5714285714286, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6320023, Length=434, Percent_Identity=30.6451612903226, Blast_Score=186, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6320764, Length=466, Percent_Identity=29.1845493562232, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI18921117, Length=424, Percent_Identity=29.7169811320755, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24639277, Length=424, Percent_Identity=29.7169811320755, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24639275, Length=424, Percent_Identity=29.7169811320755, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24653753, Length=231, Percent_Identity=25.5411255411255, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.1.28

Molecular weight: Translated: 49980; Mature: 49980

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKLKKLKDALGDKINDSIVERKLYSYDATAIPIEHTTPIAVAFPTSSEDIEMIVKICY
CCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHH
EEDIPMVPRGAGSGLTGGATPVVDGSLVISLEKMNKFHVDVGNTTAYVEPGVITSELQDY
CCCCCCCCCCCCCCCCCCCCCEECCEEEEEEECCCEEEEEECCEEEEECCCCCHHHHHHH
VESLGLFYPPDPSSFKYSTIGGNIAENAGGPRCLKYGVTREYVLGLEMYIKEGKLLKTGN
HHHCCCCCCCCCCCEEEEECCCCHHCCCCCCHHHHHCCCHHHHHHHHHHHCCCCEEECCC
PIIKDVAGYDLTRLVVGSEGTLGIVSKAILKLIPKPPYKSTIMGIFNNIENIGKSVTKIL
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TSGILPCALEFMDKNSIQAVQKFYPAGIPEDAEGVLLIELDGQKESVEKEKMEVSNILKS
HHCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
LGAGVIPAQNPQHEQKLWSARKNIGPALANIKSGKINEDIVFPRIYLSEALQKINKIAKK
HCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHHH
YNLTIVNFGHIGDGNLHVNILYDKLNKDELERAEKAVDEVFELALNYQGSITGEHGVGLT
HCEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
KKKFLEWQLKDVGIELLKAIKQSFDPKNLFNPGKVI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MEKKLKKLKDALGDKINDSIVERKLYSYDATAIPIEHTTPIAVAFPTSSEDIEMIVKICY
CCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHH
EEDIPMVPRGAGSGLTGGATPVVDGSLVISLEKMNKFHVDVGNTTAYVEPGVITSELQDY
CCCCCCCCCCCCCCCCCCCCCEECCEEEEEEECCCEEEEEECCEEEEECCCCCHHHHHHH
VESLGLFYPPDPSSFKYSTIGGNIAENAGGPRCLKYGVTREYVLGLEMYIKEGKLLKTGN
HHHCCCCCCCCCCCEEEEECCCCHHCCCCCCHHHHHCCCHHHHHHHHHHHCCCCEEECCC
PIIKDVAGYDLTRLVVGSEGTLGIVSKAILKLIPKPPYKSTIMGIFNNIENIGKSVTKIL
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TSGILPCALEFMDKNSIQAVQKFYPAGIPEDAEGVLLIELDGQKESVEKEKMEVSNILKS
HHCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
LGAGVIPAQNPQHEQKLWSARKNIGPALANIKSGKINEDIVFPRIYLSEALQKINKIAKK
HCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHHH
YNLTIVNFGHIGDGNLHVNILYDKLNKDELERAEKAVDEVFELALNYQGSITGEHGVGLT
HCEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
KKKFLEWQLKDVGIELLKAIKQSFDPKNLFNPGKVI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]