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The map label for this gene is leuC

Identifier: 195952894

GI number: 195952894

Start: 457413

End: 458687

Strand: Direct

Name: leuC

Synonym: HY04AAS1_0519

Alternate gene names: 195952894

Gene position: 457413-458687 (Clockwise)

Preceding gene: 195952893

Following gene: 195952895

Centisome position: 29.33

GC content: 39.37

Gene sequence:

>1275_bases
ATGGGACATACTATTACTGAAAAAATAATTGCAGATCATGCTGGGAAAAAGGAAGTGTTTCCTGGAGAGCTTGTTACTGC
CAAAATAGATTTGGCAATGGCCAACGATGTCACTGCTCCTTTATCCATAAAAACTTTAGAAAAATACGGCATAGAAAAAG
TATTTGACAAGGATAAAATTGCGCTTGTCCTATCTCATTTTGTTCCAGCGAAAGATATAAAATCTGCAGAACAAGCTAAA
ATAGTAAGAGATTTTGCTAAAAAACACAATATAAAATGGTTTTTTGGGGAAGGCGAAGGTATAGAGCATACATTGTTGCC
AGAAAACGGAATAGTTGTACCAGGTGATTTGGTAGTAGGAGCTGACTCTCATACTTGTACTTACGGAGGTATTGGAGCTT
TTTCTACAGGTGTAGGAAGCACAGACTTAGCCTACGCTATGGCCACTGGCGAGATATGGTTAAAAGTGCCAGAATCGATG
AAATTTATATTTTACGGAAAACTTAATAAGTGGGTTAGTGGTAAAGATTTGATACTTTATACGATAGGTCAAATAGGTGT
TGATGGTGCGCTTTATAGAGCTATGGAGTTTGATGGAGAAGCCATAAGAAGCTTGGACGTATCACAAAGACTAACCATAG
CAAATATGGCGATAGAGGCTGGCGGAAAGAGTGGTATCATATCTCCGGACGAAAAAACTATAGAATATGTAGAAAAAAGA
GCAAAAAAACCTTACAAGATATATCAAAGCGATAAAGATGCACATTATGTAGAAGTATACGAGTGGGATGCTTCTTCTAT
AGAACCTATGGTGGCATGGCCTTATTTACCCTCAAATGTACATCCAGTCTCTGAATCTACCCACATAACTATAGATCAAG
CTTTTATAGGTTCTTGTACAAATGGTCGTATTGAAGATTTAAGAATAGCAGCAAGCATATTAAAGGGTAAGAAGGTTCAT
CCTTACACGAGATGCGTTGTCATACCAGCTTCAAAAAATGTATACTTGCAAGCCCTCCATGAAGGTTTGGTAGATATATT
TATAGAAGCGGGGTGTGCCGTCAGCACATCAACATGCGGTCCTTGTTTGGGCGGGCATATGGGAATACTCGCCAAAGGAG
AAAGATGCATATCTACTTCCAATAGGAACTTTCCTGGTCGCATGGGCCATCCACAAAGCGAAGCTTATTTGGCAAACCCG
GCTGTAGTTGCTGCGAGTGCTGTTTTAGGTAGAATCGCTCATCCTGAAGAAGTAGCTTCTGAAGTATTAGTATAA

Upstream 100 bases:

>100_bases
CTAAAATTGATAAAAATGTTGTTAACGTATGAGAAAGCCACTGTTGACAATAAATTTCTCATATTGTAAAATAGTTAGCT
AGATTTTAAGAGGTGATTAT

Downstream 100 bases:

>100_bases
AGGAGGTAAATATGGATTTTCTTAAAAGCCTAACAGTTAATCAAGGCTTAAGATTGTTATCGGGTAGTGTACTTCTTTTC
GTTTTCTTATTTGGTATATT

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MGHTITEKIIADHAGKKEVFPGELVTAKIDLAMANDVTAPLSIKTLEKYGIEKVFDKDKIALVLSHFVPAKDIKSAEQAK
IVRDFAKKHNIKWFFGEGEGIEHTLLPENGIVVPGDLVVGADSHTCTYGGIGAFSTGVGSTDLAYAMATGEIWLKVPESM
KFIFYGKLNKWVSGKDLILYTIGQIGVDGALYRAMEFDGEAIRSLDVSQRLTIANMAIEAGGKSGIISPDEKTIEYVEKR
AKKPYKIYQSDKDAHYVEVYEWDASSIEPMVAWPYLPSNVHPVSESTHITIDQAFIGSCTNGRIEDLRIAASILKGKKVH
PYTRCVVIPASKNVYLQALHEGLVDIFIEAGCAVSTSTCGPCLGGHMGILAKGERCISTSNRNFPGRMGHPQSEAYLANP
AVVAASAVLGRIAHPEEVASEVLV

Sequences:

>Translated_424_residues
MGHTITEKIIADHAGKKEVFPGELVTAKIDLAMANDVTAPLSIKTLEKYGIEKVFDKDKIALVLSHFVPAKDIKSAEQAK
IVRDFAKKHNIKWFFGEGEGIEHTLLPENGIVVPGDLVVGADSHTCTYGGIGAFSTGVGSTDLAYAMATGEIWLKVPESM
KFIFYGKLNKWVSGKDLILYTIGQIGVDGALYRAMEFDGEAIRSLDVSQRLTIANMAIEAGGKSGIISPDEKTIEYVEKR
AKKPYKIYQSDKDAHYVEVYEWDASSIEPMVAWPYLPSNVHPVSESTHITIDQAFIGSCTNGRIEDLRIAASILKGKKVH
PYTRCVVIPASKNVYLQALHEGLVDIFIEAGCAVSTSTCGPCLGGHMGILAKGERCISTSNRNFPGRMGHPQSEAYLANP
AVVAASAVLGRIAHPEEVASEVLV
>Mature_423_residues
GHTITEKIIADHAGKKEVFPGELVTAKIDLAMANDVTAPLSIKTLEKYGIEKVFDKDKIALVLSHFVPAKDIKSAEQAKI
VRDFAKKHNIKWFFGEGEGIEHTLLPENGIVVPGDLVVGADSHTCTYGGIGAFSTGVGSTDLAYAMATGEIWLKVPESMK
FIFYGKLNKWVSGKDLILYTIGQIGVDGALYRAMEFDGEAIRSLDVSQRLTIANMAIEAGGKSGIISPDEKTIEYVEKRA
KKPYKIYQSDKDAHYVEVYEWDASSIEPMVAWPYLPSNVHPVSESTHITIDQAFIGSCTNGRIEDLRIAASILKGKKVHP
YTRCVVIPASKNVYLQALHEGLVDIFIEAGCAVSTSTCGPCLGGHMGILAKGERCISTSNRNFPGRMGHPQSEAYLANPA
VVAASAVLGRIAHPEEVASEVLV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=448, Percent_Identity=27.9017857142857, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI8659555, Length=386, Percent_Identity=24.6113989637306, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI41352693, Length=388, Percent_Identity=24.4845360824742, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=35.6837606837607, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI2367097, Length=472, Percent_Identity=26.9067796610169, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87081781, Length=336, Percent_Identity=24.702380952381, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1787531, Length=360, Percent_Identity=25.8333333333333, Blast_Score=82, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=443, Percent_Identity=28.8939051918736, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32564738, Length=342, Percent_Identity=31.2865497076023, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI25149342, Length=282, Percent_Identity=29.4326241134752, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=379, Percent_Identity=25.5936675461741, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320440, Length=452, Percent_Identity=35.8407079646018, Blast_Score=233, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6321429, Length=430, Percent_Identity=34.1860465116279, Blast_Score=226, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6323335, Length=417, Percent_Identity=32.1342925659472, Blast_Score=182, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6322261, Length=372, Percent_Identity=30.6451612903226, Blast_Score=160, Evalue=2e-40,
Organism=Drosophila melanogaster, GI28571643, Length=443, Percent_Identity=28.6681715575621, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI281365315, Length=444, Percent_Identity=28.6036036036036, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17864292, Length=444, Percent_Identity=28.6036036036036, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI161076999, Length=408, Percent_Identity=29.4117647058824, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17137564, Length=370, Percent_Identity=25.9459459459459, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24645686, Length=370, Percent_Identity=25.1351351351351, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_HYDS0 (B4U7U5)

Other databases:

- EMBL:   CP001130
- RefSeq:   YP_002121184.1
- ProteinModelPortal:   B4U7U5
- SMR:   B4U7U5
- GeneID:   6743315
- GenomeReviews:   CP001130_GR
- KEGG:   hya:HY04AAS1_0519
- HOGENOM:   HBG330745
- OMA:   TDPRDFL
- ProtClustDB:   PRK00402
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086
- TIGRFAMs:   TIGR02083

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 45950; Mature: 45819

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHTITEKIIADHAGKKEVFPGELVTAKIDLAMANDVTAPLSIKTLEKYGIEKVFDKDKI
CCCHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCEEEHHHHHHCHHHHHCCCHH
ALVLSHFVPAKDIKSAEQAKIVRDFAKKHNIKWFFGEGEGIEHTLLPENGIVVPGDLVVG
HHHHHHCCCHHHHCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCEEECCCEEEE
ADSHTCTYGGIGAFSTGVGSTDLAYAMATGEIWLKVPESMKFIFYGKLNKWVSGKDLILY
CCCCEEECCCCCCCCCCCCCCHHEEEEECCEEEEECCCCCEEEEEECCCCCCCCCCEEEE
TIGQIGVDGALYRAMEFDGEAIRSLDVSQRLTIANMAIEAGGKSGIISPDEKTIEYVEKR
EECCCCCCHHHHHHHHCCCHHHHHCCHHHCEEEEEEEEECCCCCCCCCCCHHHHHHHHHH
AKKPYKIYQSDKDAHYVEVYEWDASSIEPMVAWPYLPSNVHPVSESTHITIDQAFIGSCT
CCCCEEEECCCCCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCEEEEEHHEECCCC
NGRIEDLRIAASILKGKKVHPYTRCVVIPASKNVYLQALHEGLVDIFIEAGCAVSTSTCG
CCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
PCLGGHMGILAKGERCISTSNRNFPGRMGHPQSEAYLANPAVVAASAVLGRIAHPEEVAS
CHHCCCCCEEECCCEEECCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCHHHHHH
EVLV
HHCC
>Mature Secondary Structure 
GHTITEKIIADHAGKKEVFPGELVTAKIDLAMANDVTAPLSIKTLEKYGIEKVFDKDKI
CCHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCEEEHHHHHHCHHHHHCCCHH
ALVLSHFVPAKDIKSAEQAKIVRDFAKKHNIKWFFGEGEGIEHTLLPENGIVVPGDLVVG
HHHHHHCCCHHHHCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCEEECCCEEEE
ADSHTCTYGGIGAFSTGVGSTDLAYAMATGEIWLKVPESMKFIFYGKLNKWVSGKDLILY
CCCCEEECCCCCCCCCCCCCCHHEEEEECCEEEEECCCCCEEEEEECCCCCCCCCCEEEE
TIGQIGVDGALYRAMEFDGEAIRSLDVSQRLTIANMAIEAGGKSGIISPDEKTIEYVEKR
EECCCCCCHHHHHHHHCCCHHHHHCCHHHCEEEEEEEEECCCCCCCCCCCHHHHHHHHHH
AKKPYKIYQSDKDAHYVEVYEWDASSIEPMVAWPYLPSNVHPVSESTHITIDQAFIGSCT
CCCCEEEECCCCCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCEEEEEHHEECCCC
NGRIEDLRIAASILKGKKVHPYTRCVVIPASKNVYLQALHEGLVDIFIEAGCAVSTSTCG
CCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
PCLGGHMGILAKGERCISTSNRNFPGRMGHPQSEAYLANPAVVAASAVLGRIAHPEEVAS
CHHCCCCCEEECCCEEECCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCHHHHHH
EVLV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA