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The map label for this gene is hisF

Identifier: 195952823

GI number: 195952823

Start: 389997

End: 390755

Strand: Direct

Name: hisF

Synonym: HY04AAS1_0445

Alternate gene names: 195952823

Gene position: 389997-390755 (Clockwise)

Preceding gene: 195952822

Following gene: 195952830

Centisome position: 25.01

GC content: 35.31

Gene sequence:

>759_bases
ATGCTCGCTAAGAGGATTATACCATGTCTTGATATAAAAGATGGTAGGGTAGTAAAAGGTATAAACTTTGTAGATTTGAA
AGACGCTGGGGACCCAGTGGAAAATGCAATTGTTTATGAAGAGCAAAAAGCTGATGAAATAGTGTTTCTAGATATAACAG
CTTCTTATGAAAAAAGAAATACGGTGATAGATTTGGCAAAAAGAGTAGCTCAAAATATCTTCACCCCTTTTACCATAGGG
GGCGGCATAAGAACCTTAGATGATATAAGAAAACTTCTAGAAGCTGGAGCTGATAAAGTATCTATAAACTCAGCTGCTGT
TAAAAATCCTCAACTTATCTATGAGAGTGCAAGGAAATTTGGTTCTCAGTGTATAGTTGTGGCAATAGATGCAAAACACG
TGGAAGATGATACTTGGCATGTTTATATAAACGGCGGAAGATTAAATACAAACCTAGATGCTGTAGAATGGGCTAAACAA
GTGGAATCTCTTGGAGCTGGTGAGATTCTTCTAACATCTATAGATAAAGATGGTACAAAATCAGGTTACGATATAAAACT
AACAGATCTTATATCGAAAGCTGTAAATATACCAGTTATAGCCTCTGGTGGGGCTGGTAGAAAAGAGCATTTTTTAGAAG
CTTTTAAGCTAACAGAAGCAAGCGCTTGTTTAGCGGCTTCTATATTTCACTTTAAAAAGATATCTATACCAGCGTTAAAA
GCTTATTTGAAAGAAAATAACGTTCATGTTAGAATATAA

Upstream 100 bases:

>100_bases
AAAAATCCAAATGGCGTATTACCTAAAATAATACAAGAGATAAACAAAGAAACTTATCAAATAGATAATGAAACGAAAAA
TATAAATAAGGAGTTAAACA

Downstream 100 bases:

>100_bases
TTTACAAATAAAATAGGTTAGGGGTGCTATAAAAAAGTTTTTATCTTATAAAACTACGTTCTCATTGATACAAAAGCTTT
TATAAACTCTTCAGCGTTTT

Product: imidazoleglycerol phosphate synthase, cyclase subunit

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLAKRIIPCLDIKDGRVVKGINFVDLKDAGDPVENAIVYEEQKADEIVFLDITASYEKRNTVIDLAKRVAQNIFTPFTIG
GGIRTLDDIRKLLEAGADKVSINSAAVKNPQLIYESARKFGSQCIVVAIDAKHVEDDTWHVYINGGRLNTNLDAVEWAKQ
VESLGAGEILLTSIDKDGTKSGYDIKLTDLISKAVNIPVIASGGAGRKEHFLEAFKLTEASACLAASIFHFKKISIPALK
AYLKENNVHVRI

Sequences:

>Translated_252_residues
MLAKRIIPCLDIKDGRVVKGINFVDLKDAGDPVENAIVYEEQKADEIVFLDITASYEKRNTVIDLAKRVAQNIFTPFTIG
GGIRTLDDIRKLLEAGADKVSINSAAVKNPQLIYESARKFGSQCIVVAIDAKHVEDDTWHVYINGGRLNTNLDAVEWAKQ
VESLGAGEILLTSIDKDGTKSGYDIKLTDLISKAVNIPVIASGGAGRKEHFLEAFKLTEASACLAASIFHFKKISIPALK
AYLKENNVHVRI
>Mature_252_residues
MLAKRIIPCLDIKDGRVVKGINFVDLKDAGDPVENAIVYEEQKADEIVFLDITASYEKRNTVIDLAKRVAQNIFTPFTIG
GGIRTLDDIRKLLEAGADKVSINSAAVKNPQLIYESARKFGSQCIVVAIDAKHVEDDTWHVYINGGRLNTNLDAVEWAKQ
VESLGAGEILLTSIDKDGTKSGYDIKLTDLISKAVNIPVIASGGAGRKEHFLEAFKLTEASACLAASIFHFKKISIPALK
AYLKENNVHVRI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=46.3035019455253, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI87082028, Length=205, Percent_Identity=28.780487804878, Blast_Score=91, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=35.031847133758, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_HYDS0 (B4U7M4)

Other databases:

- EMBL:   CP001130
- RefSeq:   YP_002121113.1
- ProteinModelPortal:   B4U7M4
- GeneID:   6743239
- GenomeReviews:   CP001130_GR
- KEGG:   hya:HY04AAS1_0445
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27594; Mature: 27594

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDIKDGRVVKGINFVDLKDAGDPVENAIVYEEQKADEIVFLDITASYEKRN
CCHHHCCCCEECCCCCEEECCEEEEECCCCCCHHHCEEEECCCCCEEEEEEEECCCCHHH
TVIDLAKRVAQNIFTPFTIGGGIRTLDDIRKLLEAGADKVSINSAAVKNPQLIYESARKF
HHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCEEEECHHHCCCHHHHHHHHHHH
GSQCIVVAIDAKHVEDDTWHVYINGGRLNTNLDAVEWAKQVESLGAGEILLTSIDKDGTK
CCEEEEEEEECEECCCCEEEEEEECCEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
SGYDIKLTDLISKAVNIPVIASGGAGRKEHFLEAFKLTEASACLAASIFHFKKISIPALK
CCCCEEHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AYLKENNVHVRI
HHHHCCCEEEEC
>Mature Secondary Structure
MLAKRIIPCLDIKDGRVVKGINFVDLKDAGDPVENAIVYEEQKADEIVFLDITASYEKRN
CCHHHCCCCEECCCCCEEECCEEEEECCCCCCHHHCEEEECCCCCEEEEEEEECCCCHHH
TVIDLAKRVAQNIFTPFTIGGGIRTLDDIRKLLEAGADKVSINSAAVKNPQLIYESARKF
HHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCEEEECHHHCCCHHHHHHHHHHH
GSQCIVVAIDAKHVEDDTWHVYINGGRLNTNLDAVEWAKQVESLGAGEILLTSIDKDGTK
CCEEEEEEEECEECCCCEEEEEEECCEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
SGYDIKLTDLISKAVNIPVIASGGAGRKEHFLEAFKLTEASACLAASIFHFKKISIPALK
CCCCEEHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AYLKENNVHVRI
HHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA