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The map label for this gene is dosC [H]

Identifier: 195952594

GI number: 195952594

Start: 193977

End: 195065

Strand: Direct

Name: dosC [H]

Synonym: HY04AAS1_0214

Alternate gene names: 195952594

Gene position: 193977-195065 (Clockwise)

Preceding gene: 195952593

Following gene: 195952595

Centisome position: 12.44

GC content: 30.58

Gene sequence:

>1089_bases
ATGATAGACTATAAAACTAGATTGTTTGAGCTTTTAAAGAGATATCATATTAAAAAAGATGCTGCTAACATATTCTTGGA
GCTATTTGAAGCGCAAAATAATGCTGGGATTTTTGAAAACCATAACCAGCAAACTTATTACGATTTGAATATTTTGAAAA
ATTTTGGGATAACCGATAAAGATATTAAGAATTTCTGTAGAGTTAAACATATAATTTTGGACACCGACGAATGTCTTAAA
AAACGCATAAGTCCAATGTTTGATTATCTAAAGGATTTTATATACAAGTTTCTGGATTTATTTTGCAATTTTACTGAAGG
CGCTGTTTATAGCTTTATTTTAGATTGTATAGAACTTGGCTTTGCTTTGGAAAAAGAAAGTGAAAATATCTTTGATTTTT
ACAAAGCTCAAAATATTTTATCCTCGTATACTATAGACGCTATAAGAAAGTATAAAGATGATCATCAAAATGAATCCATT
GATATCTTTGGTCTTACCGCTTCTTTTGTAAAGTTTTCCCATTTTGCTTCAGAAGTAGTTTCTTTATCTTACTATGAAAA
GCTTCTTACAGACAAAGTATATGAGGTTTATGAAAAATCTACAAAAGATCAGCTTTCTAACCTTTACAATAGGGCAAAGT
TTAACGATATCAAAAATTATGAATTTGCTAGGTCAAGACGGTATAAAATACCGCTATCTCTATGCATGTTTGATATAGAT
GATTTTAAGAAAATAAACGATACTTACGGCCATCCTATTGGTGATAAGGTGATAAAAGAGCTTGGCAAGGTTGTTTTGGA
TAATGTTAGAAAATCCGATATACCCTTTAGATGGGGTGGTGAAGAATTTTTAATATTGTTTACCCATACTCATTTAAACG
AAGCTAAGTTAGCCTGTGAAAAGCTACTTAACAAGATAAGAAATATTGAAATAAAAGAAGGTGATAATATTGTTAAGTTT
ACCGTAAGCATGGGTCTAACGGAGTTAAAAGAAGAAGACGATAGTCTTGAAAAAGCCATCGAGAGGGCTGATAAAGCTCT
TTACTACGCAAAAAGAAGCGGTAAAAATCGTATTGAGATTTTGATATAA

Upstream 100 bases:

>100_bases
AAAGCTATTCAAAAAATATATCTTCTTTTTGTATAAAAACCGAAGAAAATGATATAATATTACCACAAGAGCTTATGCTT
GAAAAAATACGGGGTATATT

Downstream 100 bases:

>100_bases
ATTAAGTTGCAAAAATAACTATAAAAGCTTAAAATGTATATATGCTAACTCAAGAGATAAAAGAGGCTATAGAAGGTCAT
ATAAAGTATTTTGGGTCTAA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MIDYKTRLFELLKRYHIKKDAANIFLELFEAQNNAGIFENHNQQTYYDLNILKNFGITDKDIKNFCRVKHIILDTDECLK
KRISPMFDYLKDFIYKFLDLFCNFTEGAVYSFILDCIELGFALEKESENIFDFYKAQNILSSYTIDAIRKYKDDHQNESI
DIFGLTASFVKFSHFASEVVSLSYYEKLLTDKVYEVYEKSTKDQLSNLYNRAKFNDIKNYEFARSRRYKIPLSLCMFDID
DFKKINDTYGHPIGDKVIKELGKVVLDNVRKSDIPFRWGGEEFLILFTHTHLNEAKLACEKLLNKIRNIEIKEGDNIVKF
TVSMGLTELKEEDDSLEKAIERADKALYYAKRSGKNRIEILI

Sequences:

>Translated_362_residues
MIDYKTRLFELLKRYHIKKDAANIFLELFEAQNNAGIFENHNQQTYYDLNILKNFGITDKDIKNFCRVKHIILDTDECLK
KRISPMFDYLKDFIYKFLDLFCNFTEGAVYSFILDCIELGFALEKESENIFDFYKAQNILSSYTIDAIRKYKDDHQNESI
DIFGLTASFVKFSHFASEVVSLSYYEKLLTDKVYEVYEKSTKDQLSNLYNRAKFNDIKNYEFARSRRYKIPLSLCMFDID
DFKKINDTYGHPIGDKVIKELGKVVLDNVRKSDIPFRWGGEEFLILFTHTHLNEAKLACEKLLNKIRNIEIKEGDNIVKF
TVSMGLTELKEEDDSLEKAIERADKALYYAKRSGKNRIEILI
>Mature_362_residues
MIDYKTRLFELLKRYHIKKDAANIFLELFEAQNNAGIFENHNQQTYYDLNILKNFGITDKDIKNFCRVKHIILDTDECLK
KRISPMFDYLKDFIYKFLDLFCNFTEGAVYSFILDCIELGFALEKESENIFDFYKAQNILSSYTIDAIRKYKDDHQNESI
DIFGLTASFVKFSHFASEVVSLSYYEKLLTDKVYEVYEKSTKDQLSNLYNRAKFNDIKNYEFARSRRYKIPLSLCMFDID
DFKKINDTYGHPIGDKVIKELGKVVLDNVRKSDIPFRWGGEEFLILFTHTHLNEAKLACEKLLNKIRNIEIKEGDNIVKF
TVSMGLTELKEEDDSLEKAIERADKALYYAKRSGKNRIEILI

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=160, Percent_Identity=38.125, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=35.3658536585366, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87081881, Length=185, Percent_Identity=32.972972972973, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786584, Length=161, Percent_Identity=32.2981366459627, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787816, Length=186, Percent_Identity=31.7204301075269, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=30.5389221556886, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788085, Length=192, Percent_Identity=30.2083333333333, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788381, Length=161, Percent_Identity=25.4658385093168, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787541, Length=158, Percent_Identity=26.5822784810127, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787056, Length=175, Percent_Identity=29.7142857142857, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081974, Length=162, Percent_Identity=27.1604938271605, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787802, Length=165, Percent_Identity=29.0909090909091, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788956, Length=189, Percent_Identity=30.6878306878307, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42658; Mature: 42658

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDYKTRLFELLKRYHIKKDAANIFLELFEAQNNAGIFENHNQQTYYDLNILKNFGITDK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHCCCCHH
DIKNFCRVKHIILDTDECLKKRISPMFDYLKDFIYKFLDLFCNFTEGAVYSFILDCIELG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
FALEKESENIFDFYKAQNILSSYTIDAIRKYKDDHQNESIDIFGLTASFVKFSHFASEVV
CHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHH
SLSYYEKLLTDKVYEVYEKSTKDQLSNLYNRAKFNDIKNYEFARSRRYKIPLSLCMFDID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHCHH
DFKKINDTYGHPIGDKVIKELGKVVLDNVRKSDIPFRWGGEEFLILFTHTHLNEAKLACE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEEECCCCHHHHHHH
KLLNKIRNIEIKEGDNIVKFTVSMGLTELKEEDDSLEKAIERADKALYYAKRSGKNRIEI
HHHHHHHCCEEECCCCEEEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LI
EC
>Mature Secondary Structure
MIDYKTRLFELLKRYHIKKDAANIFLELFEAQNNAGIFENHNQQTYYDLNILKNFGITDK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHCCCCHH
DIKNFCRVKHIILDTDECLKKRISPMFDYLKDFIYKFLDLFCNFTEGAVYSFILDCIELG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
FALEKESENIFDFYKAQNILSSYTIDAIRKYKDDHQNESIDIFGLTASFVKFSHFASEVV
CHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHH
SLSYYEKLLTDKVYEVYEKSTKDQLSNLYNRAKFNDIKNYEFARSRRYKIPLSLCMFDID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHCHH
DFKKINDTYGHPIGDKVIKELGKVVLDNVRKSDIPFRWGGEEFLILFTHTHLNEAKLACE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEEECCCCHHHHHHH
KLLNKIRNIEIKEGDNIVKFTVSMGLTELKEEDDSLEKAIERADKALYYAKRSGKNRIEI
HHHHHHHCCEEECCCCEEEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LI
EC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]