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The map label for this gene is lepA

Identifier: 195952478

GI number: 195952478

Start: 91855

End: 93654

Strand: Direct

Name: lepA

Synonym: HY04AAS1_0098

Alternate gene names: 195952478

Gene position: 91855-93654 (Clockwise)

Preceding gene: 195952477

Following gene: 195952479

Centisome position: 5.89

GC content: 37.94

Gene sequence:

>1800_bases
ATGGAAAACATAAGAAACTTCTCGATAATAGCCCATGTAGACCATGGCAAATCTACATTGGCCGATAGGCTTATAGAGTT
TTGCGGAGCTATCACAGATAGAGAAAAAAAAGATCAAATGCTTGATACTCTTGATATAGAAAGAGAAAGAGGCATCACGA
TAAAACTCCAAGCTGTGAAGATAAACTATCACAGCAAGATACAAAACAAAGACTTTACATTGCATCTTATAGACACACCA
GGGCACGTAGATTTTTCTTACGAAGTATCAAGATCTTTAGCAGCTTGCGAAGGGGCTTTGCTCTTAATAGATGCCTCACA
AGGTATAGAAGCCCAAACTGTGGCAAATTTTTGGAAAGCCGTCGAGCAAAACCTAGTTATAATACCTGTAATAAACAAAA
TAGACCTACCAGCAGCAGACCCAGATCGTATAAAACTTCAAATAAGAGATATACTTGGCCTTGACCCAGATGAGGCTATA
CTTGCTTCTGCAAAAGAAGGTATAGGGATAGAAGAGATTTTAGAGGCTATCACAAAGAGGATACCACCACCAAAAGGAGA
TGAAAATGCACCTTTAAAAGCTCTCATATTTGATTCTTATTATGATCCTTATAGAGGTGCTGTGGCTTTTGTTAGAATAT
TTGATGGGTCTTTGAAAGTAGGTGATAGGATAAAGCTATTTTCTACCGGCAAGGAGTTTGAAGTTACTGAGGTTGGTGCC
CAGACGCCAAAAATGACAAAGCTTCCTATACTAAAAGCCGGAGACGTTGGGTATATAGCAGCTTCTATAAAAGATGTAAG
AGATATAAGAGTTGGCGATACTATCACATTAAAATCAAACCCCACCAAAGAAGCAATCCCTGGTTTTAAAACTGCTAAGC
CTATGGTTTTTGCCGGGCTTTACCCCACCGAGGATTCAGACTTTGAAGAGCTAAGAGATGCTTTAGAAAAATACTCTATA
AACGACGCAGCTATCACTTACGAACCAGAAACATCCCCTGCTATAGGTATGGGTTTTAGATGTGGGTTTTTGGGGCTTTT
ACACATGGAAATAGTCCAAGAAAGACTTTCAAGAGAGTACAACATAGATGTTATCACCACTGCTCCAAGCGTTGTTTATA
GGTTAAAGCTTAAAAATAGATCTGATATCTTAGAGGTCAAAAGCTCAAACGATATACCAGACAACTTTGGCCTTATAGAG
TATATAGAAGAACCATACGTATTGGCCACCATCATTACACCCAAAGACTATGTAGGAAATATAATAAATATATGCCAAGA
TAAAAGGGGTATACAGGTAAAATTTTCTTACCTTGATCAAAACACAGCGTTGCTTGAGTATGAGATGCCACTTCAAGAGA
TCATAGTGGATTTTCACGATAAGATAAAATCTGCTTCCAAAGGATATGCATCTTTTGATTATGAGTTTTTAGGATATAAA
GAATCTGATTTGGTAAAACTAACTATCATGATAAACAAAGAACCAGTGGATGCTCTGTCGTTTTTGGTGCACAAAGATAA
AGCTTACAGAAAAGCAAGGGCTTTGGTGGAGCAACTAAAAGAGACTATACCAAGACAGCTTTTTGAAATAAACGTCCAAG
CGGCTATAGGTAGTAAAATTATAGCTTCAGAGCGTATACCCCCAATGAGAGCAAACGTTACTGCAAAGTGCTATGGTGGT
GATATATCAAGAAAAAGAAAGCTCTTAGAAAAGCAAAAAGAAGGTAAAAAACGCATGAAGCAGTTTGGCAAGGTATCTTT
GCCTCAAGAGGCGTTTTTAAGCGTTTTAAAAGCCCAATGA

Upstream 100 bases:

>100_bases
GGCTTTGGCGTTGGCAGAGGACATAGAAAAAGCCCGTCAAAACGCTAAAAAAGTTGCTAATGCAGTAAGAATTTTGGAAG
AGAGTATTTAGTATAATCCG

Downstream 100 bases:

>100_bases
TAAAAACAACTGACAAGTCAAAGTGGAATGATTGGCGTTGGCAACTTCAAAACCGTATAACCACTTTAGAAGAATTATCA
AAATATATAGAGCTAACTAA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MENIRNFSIIAHVDHGKSTLADRLIEFCGAITDREKKDQMLDTLDIERERGITIKLQAVKINYHSKIQNKDFTLHLIDTP
GHVDFSYEVSRSLAACEGALLLIDASQGIEAQTVANFWKAVEQNLVIIPVINKIDLPAADPDRIKLQIRDILGLDPDEAI
LASAKEGIGIEEILEAITKRIPPPKGDENAPLKALIFDSYYDPYRGAVAFVRIFDGSLKVGDRIKLFSTGKEFEVTEVGA
QTPKMTKLPILKAGDVGYIAASIKDVRDIRVGDTITLKSNPTKEAIPGFKTAKPMVFAGLYPTEDSDFEELRDALEKYSI
NDAAITYEPETSPAIGMGFRCGFLGLLHMEIVQERLSREYNIDVITTAPSVVYRLKLKNRSDILEVKSSNDIPDNFGLIE
YIEEPYVLATIITPKDYVGNIINICQDKRGIQVKFSYLDQNTALLEYEMPLQEIIVDFHDKIKSASKGYASFDYEFLGYK
ESDLVKLTIMINKEPVDALSFLVHKDKAYRKARALVEQLKETIPRQLFEINVQAAIGSKIIASERIPPMRANVTAKCYGG
DISRKRKLLEKQKEGKKRMKQFGKVSLPQEAFLSVLKAQ

Sequences:

>Translated_599_residues
MENIRNFSIIAHVDHGKSTLADRLIEFCGAITDREKKDQMLDTLDIERERGITIKLQAVKINYHSKIQNKDFTLHLIDTP
GHVDFSYEVSRSLAACEGALLLIDASQGIEAQTVANFWKAVEQNLVIIPVINKIDLPAADPDRIKLQIRDILGLDPDEAI
LASAKEGIGIEEILEAITKRIPPPKGDENAPLKALIFDSYYDPYRGAVAFVRIFDGSLKVGDRIKLFSTGKEFEVTEVGA
QTPKMTKLPILKAGDVGYIAASIKDVRDIRVGDTITLKSNPTKEAIPGFKTAKPMVFAGLYPTEDSDFEELRDALEKYSI
NDAAITYEPETSPAIGMGFRCGFLGLLHMEIVQERLSREYNIDVITTAPSVVYRLKLKNRSDILEVKSSNDIPDNFGLIE
YIEEPYVLATIITPKDYVGNIINICQDKRGIQVKFSYLDQNTALLEYEMPLQEIIVDFHDKIKSASKGYASFDYEFLGYK
ESDLVKLTIMINKEPVDALSFLVHKDKAYRKARALVEQLKETIPRQLFEINVQAAIGSKIIASERIPPMRANVTAKCYGG
DISRKRKLLEKQKEGKKRMKQFGKVSLPQEAFLSVLKAQ
>Mature_599_residues
MENIRNFSIIAHVDHGKSTLADRLIEFCGAITDREKKDQMLDTLDIERERGITIKLQAVKINYHSKIQNKDFTLHLIDTP
GHVDFSYEVSRSLAACEGALLLIDASQGIEAQTVANFWKAVEQNLVIIPVINKIDLPAADPDRIKLQIRDILGLDPDEAI
LASAKEGIGIEEILEAITKRIPPPKGDENAPLKALIFDSYYDPYRGAVAFVRIFDGSLKVGDRIKLFSTGKEFEVTEVGA
QTPKMTKLPILKAGDVGYIAASIKDVRDIRVGDTITLKSNPTKEAIPGFKTAKPMVFAGLYPTEDSDFEELRDALEKYSI
NDAAITYEPETSPAIGMGFRCGFLGLLHMEIVQERLSREYNIDVITTAPSVVYRLKLKNRSDILEVKSSNDIPDNFGLIE
YIEEPYVLATIITPKDYVGNIINICQDKRGIQVKFSYLDQNTALLEYEMPLQEIIVDFHDKIKSASKGYASFDYEFLGYK
ESDLVKLTIMINKEPVDALSFLVHKDKAYRKARALVEQLKETIPRQLFEINVQAAIGSKIIASERIPPMRANVTAKCYGG
DISRKRKLLEKQKEGKKRMKQFGKVSLPQEAFLSVLKAQ

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=606, Percent_Identity=51.4851485148515, Blast_Score=638, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=232, Percent_Identity=33.1896551724138, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI18390331, Length=157, Percent_Identity=36.9426751592357, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=41.6666666666667, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI25306287, Length=135, Percent_Identity=41.4814814814815, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI25306283, Length=156, Percent_Identity=39.1025641025641, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI19923640, Length=156, Percent_Identity=39.1025641025641, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=41.4414414414414, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=41.4414414414414, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=41.4414414414414, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI217272892, Length=212, Percent_Identity=30.6603773584906, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI217272894, Length=212, Percent_Identity=30.6603773584906, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI53729339, Length=221, Percent_Identity=28.0542986425339, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI53729337, Length=221, Percent_Identity=28.0542986425339, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI34147630, Length=257, Percent_Identity=28.4046692607004, Blast_Score=71, Evalue=4e-12,
Organism=Homo sapiens, GI94966752, Length=196, Percent_Identity=27.0408163265306, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=53.6912751677852, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=510, Percent_Identity=26.2745098039216, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1789738, Length=177, Percent_Identity=32.2033898305085, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789559, Length=254, Percent_Identity=29.1338582677165, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790835, Length=144, Percent_Identity=31.9444444444444, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=615, Percent_Identity=43.2520325203252, Blast_Score=526, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17556745, Length=463, Percent_Identity=25.2699784017279, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17533571, Length=147, Percent_Identity=36.734693877551, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71988819, Length=214, Percent_Identity=33.1775700934579, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=214, Percent_Identity=32.7102803738318, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=158, Percent_Identity=36.0759493670886, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=146, Percent_Identity=36.3013698630137, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32566303, Length=312, Percent_Identity=27.8846153846154, Blast_Score=69, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI25141371, Length=279, Percent_Identity=26.8817204301075, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=604, Percent_Identity=44.8675496688742, Blast_Score=525, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6324707, Length=146, Percent_Identity=41.0958904109589, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=146, Percent_Identity=41.0958904109589, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323098, Length=155, Percent_Identity=35.4838709677419, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6322359, Length=138, Percent_Identity=38.4057971014493, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=40.4255319148936, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=243, Percent_Identity=30.0411522633745, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=46.523178807947, Blast_Score=573, Evalue=1e-163,
Organism=Drosophila melanogaster, GI28574573, Length=314, Percent_Identity=30.8917197452229, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24582462, Length=155, Percent_Identity=36.7741935483871, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=38.5135135135135, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=38.5135135135135, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=38.5135135135135, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI221458488, Length=156, Percent_Identity=37.1794871794872, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357743, Length=175, Percent_Identity=33.7142857142857, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281363316, Length=259, Percent_Identity=28.1853281853282, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17864358, Length=259, Percent_Identity=28.1853281853282, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28572034, Length=222, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19921738, Length=232, Percent_Identity=29.3103448275862, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_HYDS0 (B4U6M2)

Other databases:

- EMBL:   CP001130
- RefSeq:   YP_002120768.1
- GeneID:   6742881
- GenomeReviews:   CP001130_GR
- KEGG:   hya:HY04AAS1_0098
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 67212; Mature: 67212

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIRNFSIIAHVDHGKSTLADRLIEFCGAITDREKKDQMLDTLDIERERGITIKLQAVK
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHCCCEEEEEEEE
INYHSKIQNKDFTLHLIDTPGHVDFSYEVSRSLAACEGALLLIDASQGIEAQTVANFWKA
EEEHHCCCCCCEEEEEEECCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
VEQNLVIIPVINKIDLPAADPDRIKLQIRDILGLDPDEAILASAKEGIGIEEILEAITKR
HHCCEEEEEEECCCCCCCCCCCCEEEEEEHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
IPPPKGDENAPLKALIFDSYYDPYRGAVAFVRIFDGSLKVGDRIKLFSTGKEFEVTEVGA
CCCCCCCCCCCEEEEEECCCCCCHHHHEEEEEEECCCCCCCCEEEEEECCCCEEEEECCC
QTPKMTKLPILKAGDVGYIAASIKDVRDIRVGDTITLKSNPTKEAIPGFKTAKPMVFAGL
CCCCCCCCCEEECCCCCEEHHHHHHHHHEECCCEEEECCCCCHHHCCCCCCCCCEEEEEC
YPTEDSDFEELRDALEKYSINDAAITYEPETSPAIGMGFRCGFLGLLHMEIVQERLSREY
CCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
NIDVITTAPSVVYRLKLKNRSDILEVKSSNDIPDNFGLIEYIEEPYVLATIITPKDYVGN
CEEEEECCCCEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHCCCEEEEEEECCHHHHHH
IINICQDKRGIQVKFSYLDQNTALLEYEMPLQEIIVDFHDKIKSASKGYASFDYEFLGYK
HHHHHCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCC
ESDLVKLTIMINKEPVDALSFLVHKDKAYRKARALVEQLKETIPRQLFEINVQAAIGSKI
CCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHCCHH
IASERIPPMRANVTAKCYGGDISRKRKLLEKQKEGKKRMKQFGKVSLPQEAFLSVLKAQ
HHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MENIRNFSIIAHVDHGKSTLADRLIEFCGAITDREKKDQMLDTLDIERERGITIKLQAVK
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHCCCEEEEEEEE
INYHSKIQNKDFTLHLIDTPGHVDFSYEVSRSLAACEGALLLIDASQGIEAQTVANFWKA
EEEHHCCCCCCEEEEEEECCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
VEQNLVIIPVINKIDLPAADPDRIKLQIRDILGLDPDEAILASAKEGIGIEEILEAITKR
HHCCEEEEEEECCCCCCCCCCCCEEEEEEHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
IPPPKGDENAPLKALIFDSYYDPYRGAVAFVRIFDGSLKVGDRIKLFSTGKEFEVTEVGA
CCCCCCCCCCCEEEEEECCCCCCHHHHEEEEEEECCCCCCCCEEEEEECCCCEEEEECCC
QTPKMTKLPILKAGDVGYIAASIKDVRDIRVGDTITLKSNPTKEAIPGFKTAKPMVFAGL
CCCCCCCCCEEECCCCCEEHHHHHHHHHEECCCEEEECCCCCHHHCCCCCCCCCEEEEEC
YPTEDSDFEELRDALEKYSINDAAITYEPETSPAIGMGFRCGFLGLLHMEIVQERLSREY
CCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
NIDVITTAPSVVYRLKLKNRSDILEVKSSNDIPDNFGLIEYIEEPYVLATIITPKDYVGN
CEEEEECCCCEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHCCCEEEEEEECCHHHHHH
IINICQDKRGIQVKFSYLDQNTALLEYEMPLQEIIVDFHDKIKSASKGYASFDYEFLGYK
HHHHHCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCC
ESDLVKLTIMINKEPVDALSFLVHKDKAYRKARALVEQLKETIPRQLFEINVQAAIGSKI
CCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHCCHH
IASERIPPMRANVTAKCYGGDISRKRKLLEKQKEGKKRMKQFGKVSLPQEAFLSVLKAQ
HHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA