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The map label for this gene is purT [H]

Identifier: 195952477

GI number: 195952477

Start: 90661

End: 91845

Strand: Direct

Name: purT [H]

Synonym: HY04AAS1_0097

Alternate gene names: 195952477

Gene position: 90661-91845 (Clockwise)

Preceding gene: 195952476

Following gene: 195952478

Centisome position: 5.81

GC content: 38.06

Gene sequence:

>1185_bases
ATGATAGGCACCCCATTGTCTTACAACACTACAAAAATATTGCTTCTTGGTTCAGGTGAACTTGGTAAAGAGTTTGCTAT
AGAAGCTGTAAGGCTTGGTATAGAGCTTGTGGCGGTGGATTCATATCAGCACGCTCCAGCCCAACAAGTAGCCCAAAGAT
ACTACGTTATAGATATGAAGAATAAAGAGCTTTTAAAGAGTATAGCCTATAGGGAAGCCCCCGATTTTATAGTTCCTGAG
ATAGAAGCTATAAATACAGATGCCCTTATAGAGTTAGAGCAAGAAGGTTTTAATATAGTGCCCTGTGCTAAAGCTGTAAA
ATATACGATGAACCGTATAGCCATAAGGCGATTTGCTGCTGAAGAGCTAGGACTTCCTACTTCTAAGTATAAATTTGTAA
ATACGTTTGAAGAGTTAAAACAAGCTGCCAAAGAACTTGGTTATCCAGTGGTGGTAAAACCGGTTATGAGTTCTTCAGGT
AAGGGTCAGTCTATAGTTTATAAAGAACAAGATTTAGAAAAAGCTTTTTATATAGCAAGAGAAAAGGCAAGAGGTGATTC
AAAAGAACTTATCGTAGAGGAGTTTATAGATTTTGATTATGAAATTACGATGCTCACAGTAAGAACAAAAAATCAAGGGA
TTTTGTTTTGCCCGCCCATAGGTCATATACAAGTAGAAGGTGATTATTATGAAAGCTGGCAACCTCATCCAATGGATGAT
AAGCTTTTACAAAAAGCCCAAAATATAGCAAGAACCGTTGTAGAAGGACTTGGTGGGTATGGTATATTTGGATGTGAGCT
TTTTGTAAGAAAAGATGAGGTTATATTCAACGAAATATCTCCAAGACCCCACGATACTGGTATGGTGACGATGATCTCTC
AAAATATGTCTGAGTTTGAGATACATCTAAGAGCAATACTTGGACTCCCTATACATATTGAGCTTTTAGGAGCCGGAGCC
TCTTATTGTTTTCATGCAAAAACAGATGGTATAATACCAGCTTACGACAATCTTGAAGAGGCTTTATCTATACCAAACAC
AAAGCTAAGGATCTTTGGAAAGCCCATCACAAGACCAAAAAGACGTATGGGAGTGGCTTTGGCGTTGGCAGAGGACATAG
AAAAAGCCCGTCAAAACGCTAAAAAAGTTGCTAATGCAGTAAGAATTTTGGAAGAGAGTATTTAG

Upstream 100 bases:

>100_bases
AGTCCGTGTCATTTGGCACAGAGGCGTTGCAATCTAACAGATTGGTAAAAAATTTCAGTTTTTGTTAGGTGTAAGTCGAC
GGATGTGATAAAATATAATT

Downstream 100 bases:

>100_bases
TATAATCCGATGGAAAACATAAGAAACTTCTCGATAATAGCCCATGTAGACCATGGCAAATCTACATTGGCCGATAGGCT
TATAGAGTTTTGCGGAGCTA

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MIGTPLSYNTTKILLLGSGELGKEFAIEAVRLGIELVAVDSYQHAPAQQVAQRYYVIDMKNKELLKSIAYREAPDFIVPE
IEAINTDALIELEQEGFNIVPCAKAVKYTMNRIAIRRFAAEELGLPTSKYKFVNTFEELKQAAKELGYPVVVKPVMSSSG
KGQSIVYKEQDLEKAFYIAREKARGDSKELIVEEFIDFDYEITMLTVRTKNQGILFCPPIGHIQVEGDYYESWQPHPMDD
KLLQKAQNIARTVVEGLGGYGIFGCELFVRKDEVIFNEISPRPHDTGMVTMISQNMSEFEIHLRAILGLPIHIELLGAGA
SYCFHAKTDGIIPAYDNLEEALSIPNTKLRIFGKPITRPKRRMGVALALAEDIEKARQNAKKVANAVRILEESI

Sequences:

>Translated_394_residues
MIGTPLSYNTTKILLLGSGELGKEFAIEAVRLGIELVAVDSYQHAPAQQVAQRYYVIDMKNKELLKSIAYREAPDFIVPE
IEAINTDALIELEQEGFNIVPCAKAVKYTMNRIAIRRFAAEELGLPTSKYKFVNTFEELKQAAKELGYPVVVKPVMSSSG
KGQSIVYKEQDLEKAFYIAREKARGDSKELIVEEFIDFDYEITMLTVRTKNQGILFCPPIGHIQVEGDYYESWQPHPMDD
KLLQKAQNIARTVVEGLGGYGIFGCELFVRKDEVIFNEISPRPHDTGMVTMISQNMSEFEIHLRAILGLPIHIELLGAGA
SYCFHAKTDGIIPAYDNLEEALSIPNTKLRIFGKPITRPKRRMGVALALAEDIEKARQNAKKVANAVRILEESI
>Mature_394_residues
MIGTPLSYNTTKILLLGSGELGKEFAIEAVRLGIELVAVDSYQHAPAQQVAQRYYVIDMKNKELLKSIAYREAPDFIVPE
IEAINTDALIELEQEGFNIVPCAKAVKYTMNRIAIRRFAAEELGLPTSKYKFVNTFEELKQAAKELGYPVVVKPVMSSSG
KGQSIVYKEQDLEKAFYIAREKARGDSKELIVEEFIDFDYEITMLTVRTKNQGILFCPPIGHIQVEGDYYESWQPHPMDD
KLLQKAQNIARTVVEGLGGYGIFGCELFVRKDEVIFNEISPRPHDTGMVTMISQNMSEFEIHLRAILGLPIHIELLGAGA
SYCFHAKTDGIIPAYDNLEEALSIPNTKLRIFGKPITRPKRRMGVALALAEDIEKARQNAKKVANAVRILEESI

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=390, Percent_Identity=51.2820512820513, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1786733, Length=257, Percent_Identity=24.124513618677, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786216, Length=210, Percent_Identity=25.7142857142857, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324702, Length=388, Percent_Identity=24.7422680412371, Blast_Score=112, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 44219; Mature: 44219

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGTPLSYNTTKILLLGSGELGKEFAIEAVRLGIELVAVDSYQHAPAQQVAQRYYVIDMK
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCEEEEEECCCCCCCHHHHHCEEEEEECC
NKELLKSIAYREAPDFIVPEIEAINTDALIELEQEGFNIVPCAKAVKYTMNRIAIRRFAA
CHHHHHHHHHHCCCCCCCCCCEECCCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHH
EELGLPTSKYKFVNTFEELKQAAKELGYPVVVKPVMSSSGKGQSIVYKEQDLEKAFYIAR
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCEEEEEHHHHHHHHHHHH
EKARGDSKELIVEEFIDFDYEITMLTVRTKNQGILFCPPIGHIQVEGDYYESWQPHPMDD
HHCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCEEEECCCCEEEECCCHHCCCCCCCCCH
KLLQKAQNIARTVVEGLGGYGIFGCELFVRKDEVIFNEISPRPHDTGMVTMISQNMSEFE
HHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHEECCCCCCCCCCEEEEECCCHHHHH
IHLRAILGLPIHIELLGAGASYCFHAKTDGIIPAYDNLEEALSIPNTKLRIFGKPITRPK
HHHHHHHCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCHH
RRMGVALALAEDIEKARQNAKKVANAVRILEESI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIGTPLSYNTTKILLLGSGELGKEFAIEAVRLGIELVAVDSYQHAPAQQVAQRYYVIDMK
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCEEEEEECCCCCCCHHHHHCEEEEEECC
NKELLKSIAYREAPDFIVPEIEAINTDALIELEQEGFNIVPCAKAVKYTMNRIAIRRFAA
CHHHHHHHHHHCCCCCCCCCCEECCCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHH
EELGLPTSKYKFVNTFEELKQAAKELGYPVVVKPVMSSSGKGQSIVYKEQDLEKAFYIAR
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCEEEEEHHHHHHHHHHHH
EKARGDSKELIVEEFIDFDYEITMLTVRTKNQGILFCPPIGHIQVEGDYYESWQPHPMDD
HHCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCEEEECCCCEEEECCCHHCCCCCCCCCH
KLLQKAQNIARTVVEGLGGYGIFGCELFVRKDEVIFNEISPRPHDTGMVTMISQNMSEFE
HHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHEECCCCCCCCCCEEEEECCCHHHHH
IHLRAILGLPIHIELLGAGASYCFHAKTDGIIPAYDNLEEALSIPNTKLRIFGKPITRPK
HHHHHHHCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCHH
RRMGVALALAEDIEKARQNAKKVANAVRILEESI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]