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The map label for this gene is wcaJ [H]

Identifier: 194738330

GI number: 194738330

Start: 2207115

End: 2208509

Strand: Reverse

Name: wcaJ [H]

Synonym: SeSA_A2336

Alternate gene names: 194738330

Gene position: 2208509-2207115 (Counterclockwise)

Preceding gene: 194737286

Following gene: 194734330

Centisome position: 46.9

GC content: 53.26

Gene sequence:

>1395_bases
ATGACAAATCTAAAAAAGCGCGAACGAGCCAAAACCAATGCATCGTTAATCTCTATGGTGCAACGCTTTTCAGATATCAC
CATCATGTTTGGCGGGCTGTGGTTCATCTGTGAAGTCAGTGGACTGCCATTCCTGTACATGCACCTGTTGGTGGCGCTGA
TTACGCTGGTTGTTTTTCAAATGCTGGGCGGGATGACCGATTTTTATCGCTCCTGGCGCGGCGTCAAAATGTCGACAGAG
TTAACGCTGTTACTGCAAAACTGGACGCTGAGCCTGATCTTCAGCGCCGGTCTGGTGGCGTTTAATGATGACTTTGACAA
CCGCCTTGCCACCTGGCTGGCCTGGTATGCGCTGTCCAGCCTGGGTTTAGTGTTATGCCGTTCATTTATTCGTTTCGGCG
CGGGCTGGCTACGCAATCGTGGTTATAACACTCGCCGGGTCGCCGTCGCCGGAGATTTGCCTGCGGGCCAGGCGTTGCTG
GATAGCTTCCGTAATGAGCCGTGGCTGGGTTTTGAGGTGGTGGGCGTCTATCACGATCCCAAACCGGGCGGCGTCTCTGC
TGACTGGGCCGGGAACTTCGAGCAGTTAATTGACGATGCGAAAGCCTCGCGAATTCACAATGTTTATATCGCTATGTCAA
TGAGCGACGGCGCCATGGTGAAAAAACTGGTGCGCGAGCTGGCCGACACCACCTGCTCGGTGATCCTTATTCCCGATGTG
TTCACCTTTAATATTCTCCACTCGCGCGTGGAGGAGATGAGCGGCGTTCCGGTCGTTCCGCTGTATGACACGCCGCTGTC
AGGGATTAACCGTCTGCTTAAACGGGCAGAAGATATCGTGCTGGCGTCGCTGATTCTGCTGCTCATCTCACCGGTACTGT
GCTGCATTGCGCTGGCGGTCAAATTGAGCTCGCCCGGGCCGGTGATTTTCCGTCAGACGCGCTACGGGATGGACGGCAAG
CCGATCAAGGTCTGGAAGTTCCGCTCAATGAAGGTGATGGAGAACGATAAGGTCGTTACCCAGGCGACACAAAACGATCC
GCGCGTCACCCGGGTAGGTAACTTTTTGCGTCGTACCTCGCTGGATGAATTACCGCAGTTTATCAACGTATTAACCGGCG
GGATGTCTATCGTCGGTCCTCGCCCGCACGCGGTGGCGCATAACGAACAGTATCGTCAGTTGATTGAAGGTTACATGCTA
CGCCACAAGATGAAGCCGGGCATCACCGGCTGGGCGCAAATTAATGGCTGGCGCGGTGAGACCGATACGCTGGAGAAAAT
GGAAAAACGCGTGGAATTCGACCTGGAATACATCCGCGAATGGAGTATTTGGTTCGATATCAAAATCGTTTTTCTGACGA
TTTTTAAAGGGTTTGTTAATAAAGCGGCGTATTAA

Upstream 100 bases:

>100_bases
TGATGGAAGCGCGAACACGCACTCTGCTGGCGCTGCTGAATCAGTAACGTCAGATCTCCCCTTACCGCCTGCGGGTAAGG
GGCCAATCACAGGAACAACG

Downstream 100 bases:

>100_bases
GAACCTGACCCACAAGGCTATTTTACTTGCCATATTGAACCAGGGCAGCGTGTACGCTCCGGTTTTCCCGCGCTGTCCGT
GTTCAATCTGGCTGCGCCAA

Product: putative UDP-glucose lipid carrier transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTNLKKRERAKTNASLISMVQRFSDITIMFGGLWFICEVSGLPFLYMHLLVALITLVVFQMLGGMTDFYRSWRGVKMSTE
LTLLLQNWTLSLIFSAGLVAFNDDFDNRLATWLAWYALSSLGLVLCRSFIRFGAGWLRNRGYNTRRVAVAGDLPAGQALL
DSFRNEPWLGFEVVGVYHDPKPGGVSADWAGNFEQLIDDAKASRIHNVYIAMSMSDGAMVKKLVRELADTTCSVILIPDV
FTFNILHSRVEEMSGVPVVPLYDTPLSGINRLLKRAEDIVLASLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGK
PIKVWKFRSMKVMENDKVVTQATQNDPRVTRVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYML
RHKMKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSIWFDIKIVFLTIFKGFVNKAAY

Sequences:

>Translated_464_residues
MTNLKKRERAKTNASLISMVQRFSDITIMFGGLWFICEVSGLPFLYMHLLVALITLVVFQMLGGMTDFYRSWRGVKMSTE
LTLLLQNWTLSLIFSAGLVAFNDDFDNRLATWLAWYALSSLGLVLCRSFIRFGAGWLRNRGYNTRRVAVAGDLPAGQALL
DSFRNEPWLGFEVVGVYHDPKPGGVSADWAGNFEQLIDDAKASRIHNVYIAMSMSDGAMVKKLVRELADTTCSVILIPDV
FTFNILHSRVEEMSGVPVVPLYDTPLSGINRLLKRAEDIVLASLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGK
PIKVWKFRSMKVMENDKVVTQATQNDPRVTRVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYML
RHKMKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSIWFDIKIVFLTIFKGFVNKAAY
>Mature_463_residues
TNLKKRERAKTNASLISMVQRFSDITIMFGGLWFICEVSGLPFLYMHLLVALITLVVFQMLGGMTDFYRSWRGVKMSTEL
TLLLQNWTLSLIFSAGLVAFNDDFDNRLATWLAWYALSSLGLVLCRSFIRFGAGWLRNRGYNTRRVAVAGDLPAGQALLD
SFRNEPWLGFEVVGVYHDPKPGGVSADWAGNFEQLIDDAKASRIHNVYIAMSMSDGAMVKKLVRELADTTCSVILIPDVF
TFNILHSRVEEMSGVPVVPLYDTPLSGINRLLKRAEDIVLASLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKP
IKVWKFRSMKVMENDKVVTQATQNDPRVTRVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLR
HKMKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSIWFDIKIVFLTIFKGFVNKAAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=464, Percent_Identity=89.2241379310345, Blast_Score=875, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 52567; Mature: 52436

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLKKRERAKTNASLISMVQRFSDITIMFGGLWFICEVSGLPFLYMHLLVALITLVVFQ
CCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCEEEEHEECCCHHHHHHHHHHHHHHHHHH
MLGGMTDFYRSWRGVKMSTELTLLLQNWTLSLIFSAGLVAFNDDFDNRLATWLAWYALSS
HHCCHHHHHHHHCCCEECHHEEEEEHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHH
LGLVLCRSFIRFGAGWLRNRGYNTRRVAVAGDLPAGQALLDSFRNEPWLGFEVVGVYHDP
HHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCEEEEEEEECC
KPGGVSADWAGNFEQLIDDAKASRIHNVYIAMSMSDGAMVKKLVRELADTTCSVILIPDV
CCCCCCCCCCCCHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECCH
FTFNILHSRVEEMSGVPVVPLYDTPLSGINRLLKRAEDIVLASLILLLISPVLCCIALAV
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVMENDKVVTQATQNDPRVTRVGNFLRRTS
HCCCCCCEEEEECCCCCCCCCEEEEEECCEEEECCCCEEEECCCCCCCHHHHHHHHHHCC
LDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKMKPGITGWAQINGWRGE
HHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
TDTLEKMEKRVEFDLEYIREWSIWFDIKIVFLTIFKGFVNKAAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNLKKRERAKTNASLISMVQRFSDITIMFGGLWFICEVSGLPFLYMHLLVALITLVVFQ
CCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCEEEEHEECCCHHHHHHHHHHHHHHHHHH
MLGGMTDFYRSWRGVKMSTELTLLLQNWTLSLIFSAGLVAFNDDFDNRLATWLAWYALSS
HHCCHHHHHHHHCCCEECHHEEEEEHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHH
LGLVLCRSFIRFGAGWLRNRGYNTRRVAVAGDLPAGQALLDSFRNEPWLGFEVVGVYHDP
HHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCEEEEEEEECC
KPGGVSADWAGNFEQLIDDAKASRIHNVYIAMSMSDGAMVKKLVRELADTTCSVILIPDV
CCCCCCCCCCCCHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECCH
FTFNILHSRVEEMSGVPVVPLYDTPLSGINRLLKRAEDIVLASLILLLISPVLCCIALAV
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVMENDKVVTQATQNDPRVTRVGNFLRRTS
HCCCCCCEEEEECCCCCCCCCEEEEEECCEEEECCCCEEEECCCCCCCHHHHHHHHHHCC
LDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKMKPGITGWAQINGWRGE
HHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
TDTLEKMEKRVEFDLEYIREWSIWFDIKIVFLTIFKGFVNKAAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]