Click here to switch to the map view.

The map label for this gene is adh1 [H]

Identifier: 194738294

GI number: 194738294

Start: 2107354

End: 2108466

Strand: Direct

Name: adh1 [H]

Synonym: SeSA_A2222

Alternate gene names: 194738294

Gene position: 2107354-2108466 (Clockwise)

Preceding gene: 194734366

Following gene: 194737944

Centisome position: 44.75

GC content: 60.2

Gene sequence:

>1113_bases
ATGAATACCTTCTCACTACAAACGCGGTTGTACAGCGGTCAGGGCAGCCTGGCGGTGCTCAAGCGCTTTACCAATAAGCA
CATCTGGATAATCTGCGATGGCTTTCTGGCGCGCTCTCCGCTGCTGGATACCCTGCGTAACGCGCTGCCCGCAGATAACC
GCATCAGCGTCTTTAGCGAGATAACGCCGGACCCCACCATCCACACAGTGGTTCAGGGCATTGCGCAAATGCAGGCTCTG
CAACCGCAGGTGGTGATCGGTTTTGGCGGCGGCTCGGCAATGGACGCGGCGAAAGCGATTGTCTGGTTTAGCCAGCAGAG
CGGGATCAACATCGAAACCTGCGTGGCGATCCCGACCACCAGCGGCACCGGTTCGGAAGTGACCAGCGCCTGCGTAATTA
GCGACCCGGATAAAGGCATCAAGTACCCGCTGTTCAACAATGCGCTGTATCCGGATATGGCGATCCTCGACCCGGAGCTG
GTGGTCAGCGTACCGCCGCAGATTACCGCCAACACCGGCATGGACGTGCTGACCCACGCCCTGGAGGCCTGGGTGTCACC
ACACGCCAGCGACTTTACCGACGCGCTAGCGGAAAAGGCTGCCAAACTGGTGTTCCAGTATCTGCCCACGGCGGTGGAAA
AAGGCGACTGCGTGGCGACGCGCGGGAAAATGCACAATGCCTCAACGCTCGCCGGGATGGCCTTCAGCCAGGCGGGGCTG
GGGCTTAACCACGCGATAGCCCACCAGCTTGGCGGACAGTTTCATCTGCCGCACGGGCTGGCCAATGCGCTGCTGCTCAC
GGCGGTGATCCGTTTTAACGCCGGAGTTCCACGCGCCGCCAAACGCTACGCGCGGATGGCCAAAGCCTGCGGTTTTTGCC
CGGCAGAAGCCAATGATATCGCGGCAATCAATGCGCTGATTCAGCAAATCGAACTGCTTAAGCAACGCTGCGCCCTTCCC
TCACTGGCCGTTGCGCTTAAAGAAGGAAGATCCGACTTTTCCGCACGCATTCCGGCGATGGTGCAGGCTGCGCTGGCTGA
CGTTACGCTGCGCACTAACCCGCGCCCGGCCAATGCCGAGGAAATTCGTGAACTGCTGGAGGAACTGCTATGA

Upstream 100 bases:

>100_bases
CCACGCCAACCGGTGAAGGGACCACGTCAGCGCGTACTTTTGCCCGCTCCCGGCGCTGCGTACTGACCAACGGCTTTTCT
ATTCGCTAACCGAGGTCGCG

Downstream 100 bases:

>100_bases
GTACCGCCGTCAACGTCGTTGAAATGTCTTATAGCGCTGATGAAATTCGCGAGCGCGTCCGCGCAGCGGGCGTTGTCGGC
GCGGGCGGCGCAGGTTTTCC

Product: propanol dehydrogenase

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MNTFSLQTRLYSGQGSLAVLKRFTNKHIWIICDGFLARSPLLDTLRNALPADNRISVFSEITPDPTIHTVVQGIAQMQAL
QPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETCVAIPTTSGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPEL
VVSVPPQITANTGMDVLTHALEAWVSPHASDFTDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNASTLAGMAFSQAGL
GLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGVPRAAKRYARMAKACGFCPAEANDIAAINALIQQIELLKQRCALP
SLAVALKEGRSDFSARIPAMVQAALADVTLRTNPRPANAEEIRELLEELL

Sequences:

>Translated_370_residues
MNTFSLQTRLYSGQGSLAVLKRFTNKHIWIICDGFLARSPLLDTLRNALPADNRISVFSEITPDPTIHTVVQGIAQMQAL
QPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETCVAIPTTSGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPEL
VVSVPPQITANTGMDVLTHALEAWVSPHASDFTDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNASTLAGMAFSQAGL
GLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGVPRAAKRYARMAKACGFCPAEANDIAAINALIQQIELLKQRCALP
SLAVALKEGRSDFSARIPAMVQAALADVTLRTNPRPANAEEIRELLEELL
>Mature_370_residues
MNTFSLQTRLYSGQGSLAVLKRFTNKHIWIICDGFLARSPLLDTLRNALPADNRISVFSEITPDPTIHTVVQGIAQMQAL
QPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETCVAIPTTSGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPEL
VVSVPPQITANTGMDVLTHALEAWVSPHASDFTDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNASTLAGMAFSQAGL
GLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGVPRAAKRYARMAKACGFCPAEANDIAAINALIQQIELLKQRCALP
SLAVALKEGRSDFSARIPAMVQAALADVTLRTNPRPANAEEIRELLEELL

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=327, Percent_Identity=26.9113149847095, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1787493, Length=405, Percent_Identity=36.2962962962963, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082107, Length=380, Percent_Identity=37.8947368421053, Blast_Score=207, Evalue=6e-55,
Organism=Escherichia coli, GI48994951, Length=348, Percent_Identity=37.0689655172414, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1789163, Length=383, Percent_Identity=32.3759791122715, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1789386, Length=344, Percent_Identity=25.8720930232558, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17537053, Length=366, Percent_Identity=25.1366120218579, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321181, Length=375, Percent_Identity=32, Blast_Score=178, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24657991, Length=407, Percent_Identity=24.8157248157248, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 39488; Mature: 39488

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTFSLQTRLYSGQGSLAVLKRFTNKHIWIICDGFLARSPLLDTLRNALPADNRISVFSE
CCCEEEEEEEECCCCHHHHHHHHCCCEEEEEECCHHHCCHHHHHHHHHCCCCCCEEHHHC
ITPDPTIHTVVQGIAQMQALQPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETCVAIPTT
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHEEEECCCCCCEEEEEEEECC
SGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPELVVSVPPQITANTGMDVLTHA
CCCCHHHHCEEEECCCCCCCCCCCCCCCCCCCHHHCCCHHEEECCCCCCCCCCHHHHHHH
LEAWVSPHASDFTDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNASTLAGMAFSQAGL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCC
GLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGVPRAAKRYARMAKACGFCPAEANDI
CHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHH
AAINALIQQIELLKQRCALPSLAVALKEGRSDFSARIPAMVQAALADVTLRTNPRPANAE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCCCCCHH
EIRELLEELL
HHHHHHHHHC
>Mature Secondary Structure
MNTFSLQTRLYSGQGSLAVLKRFTNKHIWIICDGFLARSPLLDTLRNALPADNRISVFSE
CCCEEEEEEEECCCCHHHHHHHHCCCEEEEEECCHHHCCHHHHHHHHHCCCCCCEEHHHC
ITPDPTIHTVVQGIAQMQALQPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETCVAIPTT
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHEEEECCCCCCEEEEEEEECC
SGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPELVVSVPPQITANTGMDVLTHA
CCCCHHHHCEEEECCCCCCCCCCCCCCCCCCCHHHCCCHHEEECCCCCCCCCCHHHHHHH
LEAWVSPHASDFTDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNASTLAGMAFSQAGL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCC
GLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGVPRAAKRYARMAKACGFCPAEANDI
CHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHH
AAINALIQQIELLKQRCALPSLAVALKEGRSDFSARIPAMVQAALADVTLRTNPRPANAE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCCCCCHH
EIRELLEELL
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]