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The map label for this gene is lhgO [H]

Identifier: 194737869

GI number: 194737869

Start: 2845970

End: 2847238

Strand: Direct

Name: lhgO [H]

Synonym: SeSA_A2934

Alternate gene names: 194737869

Gene position: 2845970-2847238 (Clockwise)

Preceding gene: 194734945

Following gene: 194736886

Centisome position: 60.44

GC content: 58.79

Gene sequence:

>1269_bases
ATGTATGATTTTGTGATTATCGGCGGCGGCATTATCGGCATGTCGACCGCTATGCAACTTATTGATGTCTACCCGGATGC
GCGCATCGCGTTGTTGGAAAAAGAGTCCGCGCCAGCCTGCCACCAGACCGGGCATAACAGCGGCGTGATCCATGCTGGGG
TGTACTACACGCCCGGCAGCCTCAAAGCGCAGTTTTGTCTGGCAGGCAATCAGGCGACCAAAACCTTTTGCGATCAAAAC
AACATCCGCTACGACACCTGCGGCAAAATGTTGGTCGCCACCTCGGAGCTGGAAATGGCGCGGATGCGCGCGCTATGGGA
GCGGACGGCGGCCAACGGCTTAGAGCGCGAGTGGTTAAGCGCGGCGGAGCTGCGTGAACGCGAACCCAATATCATTGGCC
TGGGCGGGATTTTTGTCCCTTCCAGCGGGATTGTCAGCTATCGCGATGTCGCGACGGCCATGGCGAACCGCTTTCAGGCC
AAAGGCGGCGAGATCATCTACCATGCCGAGGTCAGCGCGCTGACAGAACATGCCGCTGGCATTGTTATTCGCACCTCGCA
AGGTCGGGAAATCGAAACCGCTATGCTTATCGGCTGTGCAGGGCTGATGGCGGATCGGCTGGTCAAAATGCTGGGCGTGG
AGCCCGGTTTTATTATCTGCCCGTTCCGCGGCGAGTACTTTCGTCTAGCCCCGCGACACAACCGGATCGTTAACCATTTG
ATCTACCCGATCCCCGATCCGGCAATGCCGTTTCTCGGCGTCCATCTTACCCGCATGATCGACGGCAGCGTTACGGTCGG
GCCGAATGCGGTGCTGGCGCTTAAACGCGAAGGCTACCGTAAGCGCGACGTTTCCTTCACCGACACTCTGGAGATTTTTC
GCTCCGCCGGCATCCGTCGCGTACTGCAAAACCATCTGCTTTCCGGACTGAGCGAGATGAAAAACTCGCTGTGCAAAAGC
GGCTATTTGCGGCGAGTACAAAAGTATTGCCCCAGCCTGACCGTCAACGATCTCCAGCCCTGGCCGGCAGGCGTGCGGGC
GCAGGCGGTTTCGCCGGACGGCAAACTGATTGATGATTTTCTGTTTGTCACGACGCCGCGTTCCATTCACACCTGTAACG
CCCCCTCCCCGGCGGCGACATCCGCGATACCTATCGGCGCGCATATCGTTAGCAAAGTTCAGGCGCTACGCGCAAGCCAG
AGCAACCCCGGACGTACGCTGCGTGCGGCACGCAGCGTGGACGCATTACACGCCGCATTTACCCGTTAA

Upstream 100 bases:

>100_bases
GACTTGCGTCGCGAACTGATGCGCCAGCGCGGTTACTTCGCCTACGCCGCCAGCCATTATCAAACTCACCAATAAGCGCC
ACGGCGAAGGAATTATGCGG

Downstream 100 bases:

>100_bases
CCCTTCAGACAGGAAGCAATTATGCAACTTAACGACTCTACCCTGTTCCGCCAGCAGGCCTTTATCGATGGCGACTGGCG
CGACGCGCGCGGCGGCGATG

Product: hydroxyglutarate oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESAPACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNQATKTFCDQN
NIRYDTCGKMLVATSELEMARMRALWERTAANGLEREWLSAAELREREPNIIGLGGIFVPSSGIVSYRDVATAMANRFQA
KGGEIIYHAEVSALTEHAAGIVIRTSQGREIETAMLIGCAGLMADRLVKMLGVEPGFIICPFRGEYFRLAPRHNRIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLALKREGYRKRDVSFTDTLEIFRSAGIRRVLQNHLLSGLSEMKNSLCKS
GYLRRVQKYCPSLTVNDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRSIHTCNAPSPAATSAIPIGAHIVSKVQALRASQ
SNPGRTLRAARSVDALHAAFTR

Sequences:

>Translated_422_residues
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESAPACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNQATKTFCDQN
NIRYDTCGKMLVATSELEMARMRALWERTAANGLEREWLSAAELREREPNIIGLGGIFVPSSGIVSYRDVATAMANRFQA
KGGEIIYHAEVSALTEHAAGIVIRTSQGREIETAMLIGCAGLMADRLVKMLGVEPGFIICPFRGEYFRLAPRHNRIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLALKREGYRKRDVSFTDTLEIFRSAGIRRVLQNHLLSGLSEMKNSLCKS
GYLRRVQKYCPSLTVNDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRSIHTCNAPSPAATSAIPIGAHIVSKVQALRASQ
SNPGRTLRAARSVDALHAAFTR
>Mature_422_residues
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESAPACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNQATKTFCDQN
NIRYDTCGKMLVATSELEMARMRALWERTAANGLEREWLSAAELREREPNIIGLGGIFVPSSGIVSYRDVATAMANRFQA
KGGEIIYHAEVSALTEHAAGIVIRTSQGREIETAMLIGCAGLMADRLVKMLGVEPGFIICPFRGEYFRLAPRHNRIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLALKREGYRKRDVSFTDTLEIFRSAGIRRVLQNHLLSGLSEMKNSLCKS
GYLRRVQKYCPSLTVNDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRSIHTCNAPSPAATSAIPIGAHIVSKVQALRASQ
SNPGRTLRAARSVDALHAAFTR

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=398, Percent_Identity=40.7035175879397, Blast_Score=310, Evalue=2e-84,
Organism=Escherichia coli, GI87082148, Length=422, Percent_Identity=87.914691943128, Blast_Score=759, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17565764, Length=401, Percent_Identity=41.3965087281795, Blast_Score=321, Evalue=4e-88,
Organism=Drosophila melanogaster, GI24585081, Length=406, Percent_Identity=39.6551724137931, Blast_Score=282, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 46019; Mature: 46019

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESAPACHQTGHNSGVIHAGVYYTPGS
CCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCEEEEEEEECCCC
LKAQFCLAGNQATKTFCDQNNIRYDTCGKMLVATSELEMARMRALWERTAANGLEREWLS
CEEEEEECCCCHHHHHHCCCCEEHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHH
AAELREREPNIIGLGGIFVPSSGIVSYRDVATAMANRFQAKGGEIIYHAEVSALTEHAAG
HHHHHHCCCCEEEECCEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCE
IVIRTSQGREIETAMLIGCAGLMADRLVKMLGVEPGFIICPFRGEYFRLAPRHNRIVNHL
EEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCHHHHHHHHC
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLALKREGYRKRDVSFTDTLEIFRSAGIRR
CCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
VLQNHLLSGLSEMKNSLCKSGYLRRVQKYCPSLTVNDLQPWPAGVRAQAVSPDGKLIDDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEECCCCCEECCE
LFVTTPRSIHTCNAPSPAATSAIPIGAHIVSKVQALRASQSNPGRTLRAARSVDALHAAF
EEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
TR
CC
>Mature Secondary Structure
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESAPACHQTGHNSGVIHAGVYYTPGS
CCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCEEEEEEEECCCC
LKAQFCLAGNQATKTFCDQNNIRYDTCGKMLVATSELEMARMRALWERTAANGLEREWLS
CEEEEEECCCCHHHHHHCCCCEEHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHH
AAELREREPNIIGLGGIFVPSSGIVSYRDVATAMANRFQAKGGEIIYHAEVSALTEHAAG
HHHHHHCCCCEEEECCEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCE
IVIRTSQGREIETAMLIGCAGLMADRLVKMLGVEPGFIICPFRGEYFRLAPRHNRIVNHL
EEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCHHHHHHHHC
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLALKREGYRKRDVSFTDTLEIFRSAGIRR
CCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
VLQNHLLSGLSEMKNSLCKSGYLRRVQKYCPSLTVNDLQPWPAGVRAQAVSPDGKLIDDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEECCCCCEECCE
LFVTTPRSIHTCNAPSPAATSAIPIGAHIVSKVQALRASQSNPGRTLRAARSVDALHAAF
EEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
TR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]