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The map label for this gene is wcaA [H]

Identifier: 194737530

GI number: 194737530

Start: 2219515

End: 2220426

Strand: Reverse

Name: wcaA [H]

Synonym: SeSA_A2348

Alternate gene names: 194737530

Gene position: 2220426-2219515 (Counterclockwise)

Preceding gene: 194736839

Following gene: 194738005

Centisome position: 47.15

GC content: 51.21

Gene sequence:

>912_bases
ATGGCGCGGCCTGTGACTGTAGGCCGGACAAGCGTAGCGCCATGCGGCAAAACTATCTGGGGAATAAGCATGACAACAGA
CAATCCGCTGATCTCAATTTATATGCCAACCTGGAACAGGCAGCAGTTGGCTATCCGGGCGATTAAATCAGTACTACGTC
AGGACTACCGTCACTGGGAGATGATCATCGTGGATGATTGCTCCTCGTCTTATCAGCAGCTACAACAATTTGTTGAGGAG
CTGAACGACCCGCGCGTCCGTTATACCCATAATGCCAGGAATACTGGCGCCTGCGCGGTTCGCAACCAGGCCATTATGCA
GGCGCAAGGGCATTACATTACCGGTATTGATGATGACGATGAATGGACGCCAAACCGTCTGAGCGTGTTTCTGGCCCATA
AACACCAGTTGACTACGCACGCCTTTTTGTATGCCAACGACTATGTGTGCGAGGGCGAGGTCTACTCTCAGCCTGCCAGC
CTGCCGCTGTATCCGAAATCCCCTTATTCGCGCCGCTTGTTCTACAAGCGCAATATTATTGGCAATCAGGTTTTTACCTG
GGCCTGGCGTTTTAAAGCGTGTTTGTTCGATACCGAACTCAAGGCGGCGCAGGACTACGACATTTTTCTGCGCATGGTGG
TGGAGTATGGCGAACCGTGGAAAGTCGAAGAGGCGACGCAAATTTTACATATCAACCACGGCGAGATGCAGATTACCTCT
TCGCCGAAAAAGTTCTCCGGCTATTTCCATTTTTATCGTAAGCACAAAGATAAATTTGATCGCGCCAGTAAAAAATACCA
GCTTTTTACCCTGTACCAAATTCGCAATAAGCGAATGACCTGGCGCACGTTGCTGACGTTGCTCTCCGTGCGTAATAGCA
AACGCCTGGCGGCCGGGCTGCGGGGGCGATAA

Upstream 100 bases:

>100_bases
TTCTCAACTCCATTTTCCGCCGGGCGACGGGCTACCAGGATTATGGCTATTACGAGTATGAATACCAGTCAGATTCCAAA
TAAAAAATAAGGCGTGCCGG

Downstream 100 bases:

>100_bases
CGATGCTGGAAGACTTACGCGCGAATAGCTGGAGCTTACGCCCTTGTTGCATGGTGCTGGCCTACCGTATCGCTCATTTT
TGTTCCGTCTGGCGCAAAAA

Product: putative glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MARPVTVGRTSVAPCGKTIWGISMTTDNPLISIYMPTWNRQQLAIRAIKSVLRQDYRHWEMIIVDDCSSSYQQLQQFVEE
LNDPRVRYTHNARNTGACAVRNQAIMQAQGHYITGIDDDDEWTPNRLSVFLAHKHQLTTHAFLYANDYVCEGEVYSQPAS
LPLYPKSPYSRRLFYKRNIIGNQVFTWAWRFKACLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITS
SPKKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNSKRLAAGLRGR

Sequences:

>Translated_303_residues
MARPVTVGRTSVAPCGKTIWGISMTTDNPLISIYMPTWNRQQLAIRAIKSVLRQDYRHWEMIIVDDCSSSYQQLQQFVEE
LNDPRVRYTHNARNTGACAVRNQAIMQAQGHYITGIDDDDEWTPNRLSVFLAHKHQLTTHAFLYANDYVCEGEVYSQPAS
LPLYPKSPYSRRLFYKRNIIGNQVFTWAWRFKACLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITS
SPKKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNSKRLAAGLRGR
>Mature_302_residues
ARPVTVGRTSVAPCGKTIWGISMTTDNPLISIYMPTWNRQQLAIRAIKSVLRQDYRHWEMIIVDDCSSSYQQLQQFVEEL
NDPRVRYTHNARNTGACAVRNQAIMQAQGHYITGIDDDDEWTPNRLSVFLAHKHQLTTHAFLYANDYVCEGEVYSQPASL
PLYPKSPYSRRLFYKRNIIGNQVFTWAWRFKACLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSS
PKKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNSKRLAAGLRGR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To R.meliloti exoO [H]

Homologues:

Organism=Escherichia coli, GI1788372, Length=277, Percent_Identity=91.3357400722022, Blast_Score=539, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 35609; Mature: 35478

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPVTVGRTSVAPCGKTIWGISMTTDNPLISIYMPTWNRQQLAIRAIKSVLRQDYRHWE
CCCCEECCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEE
MIIVDDCSSSYQQLQQFVEELNDPRVRYTHNARNTGACAVRNQAIMQAQGHYITGIDDDD
EEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHHHHCCCCEEECCCCCC
EWTPNRLSVFLAHKHQLTTHAFLYANDYVCEGEVYSQPASLPLYPKSPYSRRLFYKRNII
CCCCCCEEEEEEECCCCCEEEEEEECCEEECCHHHCCCCCCCCCCCCCCHHHHEEECCCC
GNQVFTWAWRFKACLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITS
CCCCEEHHHHHHHHEECCHHCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEC
SPKKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNSKRLAAGL
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHCCCHHHHHHHHHHHHCCCHHHHCCC
RGR
CCC
>Mature Secondary Structure 
ARPVTVGRTSVAPCGKTIWGISMTTDNPLISIYMPTWNRQQLAIRAIKSVLRQDYRHWE
CCCEECCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEE
MIIVDDCSSSYQQLQQFVEELNDPRVRYTHNARNTGACAVRNQAIMQAQGHYITGIDDDD
EEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHHHHCCCCEEECCCCCC
EWTPNRLSVFLAHKHQLTTHAFLYANDYVCEGEVYSQPASLPLYPKSPYSRRLFYKRNII
CCCCCCEEEEEEECCCCCEEEEEEECCEEECCHHHCCCCCCCCCCCCCCHHHHEEECCCC
GNQVFTWAWRFKACLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITS
CCCCEEHHHHHHHHEECCHHCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEC
SPKKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNSKRLAAGL
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHCCCHHHHHHHHHHHHCCCHHHHCCC
RGR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]