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The map label for this gene is lpdA [H]

Identifier: 194737352

GI number: 194737352

Start: 180891

End: 182315

Strand: Direct

Name: lpdA [H]

Synonym: SeSA_A0173

Alternate gene names: 194737352

Gene position: 180891-182315 (Clockwise)

Preceding gene: 194736485

Following gene: 194737736

Centisome position: 3.84

GC content: 54.39

Gene sequence:

>1425_bases
ATGAGTACTGAAATCAAAACTCAGGTCGTGGTACTTGGGGCGGGCCCGGCAGGCTATTCTGCCGCTTTCCGTTGCGCTGA
TTTAGGTCTGGAAACCGTAATTGTAGAACGTTACAGCACCCTTGGCGGTGTTTGTCTGAACGTCGGCTGTATCCCTTCTA
AAGCGCTGCTGCACGTAGCAAAAGTTATCGAAGAAGCCAAAGCGCTGGCTGAACACGGTATCGTCTTCGGTGAGCCGAAA
ACCGATATCGACAAGATTCGTACCTGGAAAGAGAAAGTGATCACGCAGCTGACCGGCGGTCTGGCGGGTATGGCCAAAGG
CCGTAAAGTGAAAGTGGTCAACGGTCTGGGTAAATTCACCGGGGCGAACACCCTGGAAGTTGAAGGCGAAAACGGCAAAA
CCGTGATCAACTTCGACAACGCGATTATCGCGGCAGGTTCTCGTCCGATCCAATTACCGTTTATTCCGCATGAAGACCCG
CGCGTATGGGATTCTACCGACGCTCTGGAACTGAAAGAAGTGCCGAAGCGTATGCTGGTCATGGGCGGCGGTATCATCGG
TCTGGAAATGGGTACCGTATACCACGCGCTGGGTTCAGAGATTGACGTGGTTGAAATGTTCGACCAGGTTATCCCGGCGG
CTGATAAAGACATCGTTAAAGTCTTCACCAAGCGCATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTAACTGCC
GTTGAAGCGAAAGAAGACGGTATCTACGTTTCGATGGAAGGCAAAAAAGCCCCGGCTGAAGCGCAGCGCTATGATGCGGT
TCTGGTGGCGATCGGTCGTGTGCCGAACGGTAAAAACCTCGACGCGGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGCT
TTATCCGCGTCGACAAACAGCTGCGCACCAACGTGCCGCACATCTTCGCTATCGGCGATATCGTCGGTCAGCCGATGCTG
GCGCATAAAGGCGTTCACGAAGGCCACGTTGCCGCTGAAGTTATCGCCGGGAAGAAACACTACTTCGATCCGAAGGTTAT
CCCGTCTATCGCGTACACCGAGCCAGAAGTGGCATGGGTTGGCCTGACCGAGAAAGAAGCGAAAGAAAAAGGCATCAGCT
ATGAAACCGCCACCTTCCCGTGGGCAGCTTCTGGCCGTGCTATCGCTTCTGACTGCGCAGACGGTATGACCAAACTGATT
TTCGACAAAGAATCTCACCGCGTGATCGGCGGGGCGATTGTCGGCACCAACGGCGGCGAGCTGCTGGGTGAAATCGGTCT
GGCGATCGAAATGGGCTGTGACGCGGAAGACATCGCGCTGACCATTCATGCGCACCCGACTCTGCATGAGTCCGTGGGCC
TGGCGGCGGAAATCTTCGAAGGTAGCATTACTGACCTGCCGAACCCGAAAGCGAAGAAGAAATAA

Upstream 100 bases:

>100_bases
AATTGTTAACAATTTTGTAAGATACGGGCGGATAGAACGACCCGGTGGATGATGGGCGATCAAGTACCCCGGACCGCCGG
ATACAAATAAAGAGGTCATG

Downstream 100 bases:

>100_bases
TCTTCTGTAGGTGGCAATGCGCCATTCGACACAGAAAAGCGGCTTTACAGCCGCTTTTTTTATGCATGGCAAATCAGATG
TGTCAAATGATGGCGAAGGA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPK
TDIDKIRTWKEKVITQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
RVWDSTDALELKEVPKRMLVMGGGIIGLEMGTVYHALGSEIDVVEMFDQVIPAADKDIVKVFTKRISKKFNLMLETKVTA
VEAKEDGIYVSMEGKKAPAEAQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPML
AHKGVHEGHVAAEVIAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKLI
FDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEIFEGSITDLPNPKAKKK

Sequences:

>Translated_474_residues
MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPK
TDIDKIRTWKEKVITQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
RVWDSTDALELKEVPKRMLVMGGGIIGLEMGTVYHALGSEIDVVEMFDQVIPAADKDIVKVFTKRISKKFNLMLETKVTA
VEAKEDGIYVSMEGKKAPAEAQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPML
AHKGVHEGHVAAEVIAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKLI
FDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEIFEGSITDLPNPKAKKK
>Mature_473_residues
STEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKT
DIDKIRTWKEKVITQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDPR
VWDSTDALELKEVPKRMLVMGGGIIGLEMGTVYHALGSEIDVVEMFDQVIPAADKDIVKVFTKRISKKFNLMLETKVTAV
EAKEDGIYVSMEGKKAPAEAQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLA
HKGVHEGHVAAEVIAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKLIF
DKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEIFEGSITDLPNPKAKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=43.6123348017621, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI50301238, Length=453, Percent_Identity=28.476821192053, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI22035672, Length=429, Percent_Identity=29.1375291375291, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI33519430, Length=428, Percent_Identity=27.5700934579439, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI33519428, Length=428, Percent_Identity=27.5700934579439, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI33519426, Length=428, Percent_Identity=27.5700934579439, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI148277071, Length=428, Percent_Identity=27.5700934579439, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI148277065, Length=428, Percent_Identity=27.5700934579439, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI291045266, Length=454, Percent_Identity=27.7533039647577, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI291045268, Length=441, Percent_Identity=26.0770975056689, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=98.1012658227848, Blast_Score=944, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=26.9807280513919, Blast_Score=185, Evalue=7e-48,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=27.9120879120879, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1789915, Length=444, Percent_Identity=28.1531531531532, Blast_Score=143, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=448, Percent_Identity=39.9553571428571, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=26.8348623853211, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=436, Percent_Identity=26.8348623853211, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17557007, Length=492, Percent_Identity=26.8292682926829, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=441, Percent_Identity=26.3038548752834, Blast_Score=117, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=40.5701754385965, Blast_Score=308, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=26.6524520255864, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=471, Percent_Identity=27.8131634819533, Blast_Score=152, Evalue=8e-38,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=39.8249452954048, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24640549, Length=462, Percent_Identity=29.4372294372294, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24640553, Length=462, Percent_Identity=29.4372294372294, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24640551, Length=462, Percent_Identity=29.4372294372294, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=26.5135699373695, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50627; Mature: 50496

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVA
CCCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVITQLTGGLAGMAKGRKVKVVNGLGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCHHCC
GANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDPRVWDSTDALELKEVPKRMLV
CCCEEEEECCCCCEEEEECCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
MGGGIIGLEMGTVYHALGSEIDVVEMFDQVIPAADKDIVKVFTKRISKKFNLMLETKVTA
HCCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVSMEGKKAPAEAQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQ
EEECCCCEEEEECCCCCCCHHHHHCEEEEEEECCCCCCCCCCCCCCCEECCCCEEEECHH
LRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGKKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCEECCCCCEEEE
GLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKLIFDKESHRVIGGAIVGTNGGE
ECCHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEIFEGSITDLPNPKAKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVA
CCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVITQLTGGLAGMAKGRKVKVVNGLGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCHHCC
GANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDPRVWDSTDALELKEVPKRMLV
CCCEEEEECCCCCEEEEECCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
MGGGIIGLEMGTVYHALGSEIDVVEMFDQVIPAADKDIVKVFTKRISKKFNLMLETKVTA
HCCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVSMEGKKAPAEAQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQ
EEECCCCEEEEECCCCCCCHHHHHCEEEEEEECCCCCCCCCCCCCCCEECCCCEEEECHH
LRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGKKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCEECCCCCEEEE
GLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKLIFDKESHRVIGGAIVGTNGGE
ECCHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEIFEGSITDLPNPKAKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]