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The map label for this gene is aceF [H]

Identifier: 194736485

GI number: 194736485

Start: 178805

End: 180691

Strand: Direct

Name: aceF [H]

Synonym: SeSA_A0172

Alternate gene names: 194736485

Gene position: 178805-180691 (Clockwise)

Preceding gene: 194737204

Following gene: 194737352

Centisome position: 3.8

GC content: 56.01

Gene sequence:

>1887_bases
ATGGCTATCGAAATCAAAGTACCGGACATCGGGACAGATGAAGTTGAAATCACCGAGATTCTGGTCAAAGTGGGCGACAA
AGTGGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTCCCGTCTCCGCAGGCTGGCGTCG
TGAAAGAGATCAAAGTCTCTGTCGGCGACAAAACCGAGACCGGTGCACTTATCATGATTTTCGATTCCGCCGACGGTGCA
GCTGACGCTGCACCTGCTAAGGCAGAAGAGAAGAAAGAAGCGGCTCCGGCAGCAGCACCTGCTGCGGCAGCGGCGAAAGA
CGTTCACGTTCCGGATATCGGCAGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACACCGTTGAAGCTG
AACAGTCGCTGATCACCGTCGAAGGTGACAAGGCCTCTATGGAAGTGCCGGCGCCGTTCGCGGGCACCGTGAAAGAGATC
AAAGTGAACACCGGTGATAAAGTGTCTACCGGCTCACTGATTATGGTCTTCGAAGTGGCGGGCGCAGCGCCTGCCGCAGC
ACCAGCGAAAGCGGAGGCCGCTCCGGCAGCGGCTGCTCCAGCAGCGACGGGCGTGAAAGACGTTAACGTACCGGACATCG
GCGGCGACGAAGTTGAAGTCACCGAAGTGATGGTCAAAGTCGGCGATAAAGTTGCCGCTGAACAGTCGCTGATCACCGTG
GAAGGCGACAAAGCCTCCATGGAAGTGCCTGCGCCGTTCGCGGGCACCGTGAAAGAAATCAAAATCAGCACCGGCGACAA
AGTGAAAACCGGTTCTCTGATTATGGTCTTCGAAGTAGAAGGCGCAGCGCCTGCGGCCGCTCCGGCTAAACAGGAAGCGG
CGGCACCGGCTCCGGCGGCGAAAGCTGAGAAGCCTGCTGCCCCGGCTGCCAAAGCGGAAGGCAAGTCTGAGTTCGCTGAA
AACGACGCTTACGTTCACGCTACTCCGCTGATTCGCCGCCTGGCGCGTGAGTTTGGCGTCAACCTGGCGAAAGTGAAAGG
GACTGGTCGTAAAGGCCGTATTCTGCGCGAAGACGTTCAGGCTTACGTGAAAGATGCTATCAAACGCGCGGAAGCTGCGC
CGGCTGCTGCGGGCGGCGGTATCCCGGGTATGCTGCCGTGGCCGAAAGTGGACTTCAGCAAGTTTGGTGAAGTTGAAGAA
GTGGAACTGGGCCGTATCCAGAAAATCTCTGGCGCGAACCTGAGCCGTAACTGGGTGATGATCCCGCACGTTACGCACTT
CGACAAAACCGATATCACCGATCTGGAAGCGTTCCGTAAACAGCAGAACGCCGAAGCTGAGAAGCGCAAACTGGATGTGA
AATACACGCCAGTGGTCTTCATCATGAAAGCGGTTGCCGCTGCTCTGGAACAGATGCCGCGCTTCAACAGCTCTCTGTCC
GAAGACGGTCAGCGTCTGACCCTGAAAAAATATATCAACATCGGCGTGGCGGTTGATACCCCGAATGGCCTGGTGGTTCC
GGTCTTTAAAGACGTGAATAAGAAGAGCGTGACCGAGCTGTCTCGCGAACTGACCACCATCTCCAAAAAAGCGCGTGATG
GTAAGCTGACCGCTGGCGAAATGCAGGGTGGTTGCTTCACTATCTCCAGCATCGGCGGCCTGGGAACTACCCACTTCGCG
CCGATTGTTAACGCGCCGGAAGTGGCTATCCTCGGCGTTTCTAAGTCGGCGATGGAACCGGTGTGGAATGGGAAAGAGTT
TGTGCCGCGTCTGATGATGCCTATCTCTCTTTCCTTCGACCACCGTGTGATCGATGGTGCTGATGGTGCGCGTTTCATTA
CCATTATCAACAATATGTTGTCTGACATTCGCCGTCTGGTGATGTAA

Upstream 100 bases:

>100_bases
GTGGCGAAATCGACAAGAAAGTGGTAGCGGATGCGATTACCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTG
GCGTAAGAGGTAAAAGAATA

Downstream 100 bases:

>100_bases
TGAAAAAGCCGGCCCGACGGCCGGCTTTTTTTGATAATCTCATGTATTTTGTGAGGTTATTAGCGAAAGCGATAATTCGT
GATCCGTTTGTTGTTTCAAA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MAIEIKVPDIGTDEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVSVGDKTETGALIMIFDSADGA
ADAAPAKAEEKKEAAPAAAPAAAAAKDVHVPDIGSDEVEVTEVMVKVGDTVEAEQSLITVEGDKASMEVPAPFAGTVKEI
KVNTGDKVSTGSLIMVFEVAGAAPAAAPAKAEAAPAAAAPAATGVKDVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLITV
EGDKASMEVPAPFAGTVKEIKISTGDKVKTGSLIMVFEVEGAAPAAAPAKQEAAAPAPAAKAEKPAAPAAKAEGKSEFAE
NDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQAYVKDAIKRAEAAPAAAGGGIPGMLPWPKVDFSKFGEVEE
VELGRIQKISGANLSRNWVMIPHVTHFDKTDITDLEAFRKQQNAEAEKRKLDVKYTPVVFIMKAVAAALEQMPRFNSSLS
EDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKSVTELSRELTTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFA
PIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMMPISLSFDHRVIDGADGARFITIINNMLSDIRRLVM

Sequences:

>Translated_628_residues
MAIEIKVPDIGTDEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVSVGDKTETGALIMIFDSADGA
ADAAPAKAEEKKEAAPAAAPAAAAAKDVHVPDIGSDEVEVTEVMVKVGDTVEAEQSLITVEGDKASMEVPAPFAGTVKEI
KVNTGDKVSTGSLIMVFEVAGAAPAAAPAKAEAAPAAAAPAATGVKDVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLITV
EGDKASMEVPAPFAGTVKEIKISTGDKVKTGSLIMVFEVEGAAPAAAPAKQEAAAPAPAAKAEKPAAPAAKAEGKSEFAE
NDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQAYVKDAIKRAEAAPAAAGGGIPGMLPWPKVDFSKFGEVEE
VELGRIQKISGANLSRNWVMIPHVTHFDKTDITDLEAFRKQQNAEAEKRKLDVKYTPVVFIMKAVAAALEQMPRFNSSLS
EDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKSVTELSRELTTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFA
PIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMMPISLSFDHRVIDGADGARFITIINNMLSDIRRLVM
>Mature_627_residues
AIEIKVPDIGTDEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVSVGDKTETGALIMIFDSADGAA
DAAPAKAEEKKEAAPAAAPAAAAAKDVHVPDIGSDEVEVTEVMVKVGDTVEAEQSLITVEGDKASMEVPAPFAGTVKEIK
VNTGDKVSTGSLIMVFEVAGAAPAAAPAKAEAAPAAAAPAATGVKDVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLITVE
GDKASMEVPAPFAGTVKEIKISTGDKVKTGSLIMVFEVEGAAPAAAPAKQEAAAPAPAAKAEKPAAPAAKAEGKSEFAEN
DAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQAYVKDAIKRAEAAPAAAGGGIPGMLPWPKVDFSKFGEVEEV
ELGRIQKISGANLSRNWVMIPHVTHFDKTDITDLEAFRKQQNAEAEKRKLDVKYTPVVFIMKAVAAALEQMPRFNSSLSE
DGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKSVTELSRELTTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAP
IVNAPEVAILGVSKSAMEPVWNGKEFVPRLMMPISLSFDHRVIDGADGARFITIINNMLSDIRRLVM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=423, Percent_Identity=30.2600472813239, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI31711992, Length=406, Percent_Identity=31.7733990147783, Blast_Score=156, Evalue=7e-38,
Organism=Homo sapiens, GI19923748, Length=205, Percent_Identity=34.6341463414634, Blast_Score=126, Evalue=8e-29,
Organism=Homo sapiens, GI203098816, Length=444, Percent_Identity=26.5765765765766, Blast_Score=122, Evalue=2e-27,
Organism=Homo sapiens, GI203098753, Length=444, Percent_Identity=26.8018018018018, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI260898739, Length=169, Percent_Identity=34.9112426035503, Blast_Score=94, Evalue=5e-19,
Organism=Escherichia coli, GI1786305, Length=630, Percent_Identity=92.5396825396825, Blast_Score=1003, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=405, Percent_Identity=31.358024691358, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=401, Percent_Identity=30.6733167082294, Blast_Score=179, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17560088, Length=429, Percent_Identity=27.972027972028, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25146366, Length=207, Percent_Identity=36.7149758454106, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17538894, Length=310, Percent_Identity=29.3548387096774, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=413, Percent_Identity=28.5714285714286, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324258, Length=429, Percent_Identity=28.4382284382284, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI18859875, Length=426, Percent_Identity=30.2816901408451, Blast_Score=175, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24645909, Length=215, Percent_Identity=34.4186046511628, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=29.7872340425532, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=29.7872340425532, Blast_Score=113, Evalue=5e-25,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 65922; Mature: 65791

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEIKVPDIGTDEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVS
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEE
VGDKTETGALIMIFDSADGAADAAPAKAEEKKEAAPAAAPAAAAAKDVHVPDIGSDEVEV
CCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHH
TEVMVKVGDTVEAEQSLITVEGDKASMEVPAPFAGTVKEIKVNTGDKVSTGSLIMVFEVA
HHHHHHCCCCCCCCCCEEEEECCCCCEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEEC
GAAPAAAPAKAEAAPAAAAPAATGVKDVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLITV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCCEEEEE
EGDKASMEVPAPFAGTVKEIKISTGDKVKTGSLIMVFEVEGAAPAAAPAKQEAAAPAPAA
ECCCCCEECCCCCCCCEEEEEECCCCCEECCCEEEEEEECCCCCCCCCCHHHCCCCCCCC
KAEKPAAPAAKAEGKSEFAENDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQ
CCCCCCCCCHHCCCCHHHHCCCCEEEHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHH
AYVKDAIKRAEAAPAAAGGGIPGMLPWPKVDFSKFGEVEEVELGRIQKISGANLSRNWVM
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCEECCCCEEEECCCCCCCCEEE
IPHVTHFDKTDITDLEAFRKQQNAEAEKRKLDVKYTPVVFIMKAVAAALEQMPRFNSSLS
ECCEECCCCCCCHHHHHHHHHHCCCHHHHCCCCEECHHHHHHHHHHHHHHHCCCCCCCHH
EDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKSVTELSRELTTISKKARDGKLTAGE
CCCCEEEEEHEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEECC
MQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMMPISLSFD
CCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCHHHHHHHHCCEEECCC
HRVIDGADGARFITIINNMLSDIRRLVM
CEEEECCCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AIEIKVPDIGTDEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVS
EEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEE
VGDKTETGALIMIFDSADGAADAAPAKAEEKKEAAPAAAPAAAAAKDVHVPDIGSDEVEV
CCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHH
TEVMVKVGDTVEAEQSLITVEGDKASMEVPAPFAGTVKEIKVNTGDKVSTGSLIMVFEVA
HHHHHHCCCCCCCCCCEEEEECCCCCEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEEC
GAAPAAAPAKAEAAPAAAAPAATGVKDVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLITV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCCEEEEE
EGDKASMEVPAPFAGTVKEIKISTGDKVKTGSLIMVFEVEGAAPAAAPAKQEAAAPAPAA
ECCCCCEECCCCCCCCEEEEEECCCCCEECCCEEEEEEECCCCCCCCCCHHHCCCCCCCC
KAEKPAAPAAKAEGKSEFAENDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQ
CCCCCCCCCHHCCCCHHHHCCCCEEEHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHH
AYVKDAIKRAEAAPAAAGGGIPGMLPWPKVDFSKFGEVEEVELGRIQKISGANLSRNWVM
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCEECCCCEEEECCCCCCCCEEE
IPHVTHFDKTDITDLEAFRKQQNAEAEKRKLDVKYTPVVFIMKAVAAALEQMPRFNSSLS
ECCEECCCCCCCHHHHHHHHHHCCCHHHHCCCCEECHHHHHHHHHHHHHHHCCCCCCCHH
EDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKSVTELSRELTTISKKARDGKLTAGE
CCCCEEEEEHEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEECC
MQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMMPISLSFD
CCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCHHHHHHHHCCEEECCC
HRVIDGADGARFITIINNMLSDIRRLVM
CEEEECCCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]