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The map label for this gene is lepB [H]

Identifier: 194737191

GI number: 194737191

Start: 2726887

End: 2727861

Strand: Reverse

Name: lepB [H]

Synonym: SeSA_A2826

Alternate gene names: 194737191

Gene position: 2727861-2726887 (Counterclockwise)

Preceding gene: 194735591

Following gene: 194733998

Centisome position: 57.93

GC content: 53.23

Gene sequence:

>975_bases
ATGGCGAACATGTTTGCCCTGATTCTGGTGATAGCCACACTGGTGACGGGCATTTTATGGTGCGTTGATAAGTTTGTTTT
CGCGCCAAAACGTCGGGCGCGCCAGGCTGCCGCGCAAACGGCGTCGGGAGATGCGCTGGATAACGCTACGCTCAATAAAG
TGTCGCCTAAGCCGGGCTGGCTGGAGACCGGGGCGTCGGTTTTCCCGGTTCTGGCGATCGTTCTGATCGTTCGTTCATTT
CTTTATGAACCCTTTCAGATCCCGTCAGGCTCAATGATGCCGACACTGCTTATCGGCGATTTTATTCTGGTGGAAAAATT
TGCCTACGGCATTAAAGATCCGATCTACCAGAAAACCCTGATTGAAACCGGTCATCCAAAGCGCGGGGATATTGTGGTAT
TTAAATATCCGGAAGATCCTAAGTTAGATTACATCAAACGCGCCGTCGGTTTGCCGGGCGATAAAATCACTTATGATCCG
GTTGCAAAAGAGGTGACGATTCAGCCTGGCTGTAGCTCCGGTCAGGCGTGCGAAAATGCGCTGCCGGTTACCTACTCTAA
CGTTGAGCCGAGCGATTTTGTACAGACCTTTGCCCGCCGTAACGGCGGAGAAGCGACCAGCGGTTTCTTTGAGGTTCCGC
TAAACGAGACAAAAGAAAACGGCATTCGCCTGACCGAACGTAAAGAGACGTTAGGCGATGTGACCCACCGCATCCTGATG
GTGCCGATAGCTCAGGATCAGTTGGGCATGTATTACCAACAGCCAGGGCAACCGCTGGCGACCTGGGTTGTACCGCCGGG
GCAATATTTCATGATGGGCGACAACCGCGATAACAGCGCGGATAGCCGTTACTGGGGATTTGTTCCGGAAGCGAATCTGG
TCGGTAAAGCGGTCGCTATCTGGATGAGCTTTGACAAGCAGGAAGGGGAGTGGCCGACAGGCGTACGCCTGAGTCGTATC
GGCGGTATTCACTAA

Upstream 100 bases:

>100_bases
AAACGCATGAAGCAGATCGGTAACGTCGAGCTGCCTCAGGAGGCGTTCCTAGCCATTCTGCATGTCGGTAAAGACAATAA
ATAATCCCTAAGGAGTTGGC

Downstream 100 bases:

>100_bases
CTGTGACGAAATGATCGTTCACGCTGCCGTCTTTTTAGCGGCAGCGTGAATTATTTCCTGGATAAATTCCCTCAGACTAA
CGACATCCCCTGTCGTTGTG

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MANMFALILVIATLVTGILWCVDKFVFAPKRRARQAAAQTASGDALDNATLNKVSPKPGWLETGASVFPVLAIVLIVRSF
LYEPFQIPSGSMMPTLLIGDFILVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKITYDP
VAKEVTIQPGCSSGQACENALPVTYSNVEPSDFVQTFARRNGGEATSGFFEVPLNETKENGIRLTERKETLGDVTHRILM
VPIAQDQLGMYYQQPGQPLATWVVPPGQYFMMGDNRDNSADSRYWGFVPEANLVGKAVAIWMSFDKQEGEWPTGVRLSRI
GGIH

Sequences:

>Translated_324_residues
MANMFALILVIATLVTGILWCVDKFVFAPKRRARQAAAQTASGDALDNATLNKVSPKPGWLETGASVFPVLAIVLIVRSF
LYEPFQIPSGSMMPTLLIGDFILVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKITYDP
VAKEVTIQPGCSSGQACENALPVTYSNVEPSDFVQTFARRNGGEATSGFFEVPLNETKENGIRLTERKETLGDVTHRILM
VPIAQDQLGMYYQQPGQPLATWVVPPGQYFMMGDNRDNSADSRYWGFVPEANLVGKAVAIWMSFDKQEGEWPTGVRLSRI
GGIH
>Mature_323_residues
ANMFALILVIATLVTGILWCVDKFVFAPKRRARQAAAQTASGDALDNATLNKVSPKPGWLETGASVFPVLAIVLIVRSFL
YEPFQIPSGSMMPTLLIGDFILVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKITYDPV
AKEVTIQPGCSSGQACENALPVTYSNVEPSDFVQTFARRNGGEATSGFFEVPLNETKENGIRLTERKETLGDVTHRILMV
PIAQDQLGMYYQQPGQPLATWVVPPGQYFMMGDNRDNSADSRYWGFVPEANLVGKAVAIWMSFDKQEGEWPTGVRLSRIG
GIH

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=324, Percent_Identity=93.2098765432099, Blast_Score=623, Evalue=1e-180,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR019533
- InterPro:   IPR019766 [H]

Pfam domain/function: PF00717 Peptidase_S24; PF10502 Peptidase_S26 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 35795; Mature: 35663

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00501 SPASE_I_1 ; PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANMFALILVIATLVTGILWCVDKFVFAPKRRARQAAAQTASGDALDNATLNKVSPKPGW
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
LETGASVFPVLAIVLIVRSFLYEPFQIPSGSMMPTLLIGDFILVEKFAYGIKDPIYQKTL
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
IETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKITYDPVAKEVTIQPGCSSGQACENA
HHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCEEECCCCCEEEECCCCCCCCHHHCC
LPVTYSNVEPSDFVQTFARRNGGEATSGFFEVPLNETKENGIRLTERKETLGDVTHRILM
CCEEECCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCEEEEHHHHHHHHHHHEEEE
VPIAQDQLGMYYQQPGQPLATWVVPPGQYFMMGDNRDNSADSRYWGFVPEANLVGKAVAI
EEECHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCEECCCCCCHHHHHHEEE
WMSFDKQEGEWPTGVRLSRIGGIH
EEEECCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
ANMFALILVIATLVTGILWCVDKFVFAPKRRARQAAAQTASGDALDNATLNKVSPKPGW
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
LETGASVFPVLAIVLIVRSFLYEPFQIPSGSMMPTLLIGDFILVEKFAYGIKDPIYQKTL
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
IETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKITYDPVAKEVTIQPGCSSGQACENA
HHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCEEECCCCCEEEECCCCCCCCHHHCC
LPVTYSNVEPSDFVQTFARRNGGEATSGFFEVPLNETKENGIRLTERKETLGDVTHRILM
CCEEECCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCEEEEHHHHHHHHHHHEEEE
VPIAQDQLGMYYQQPGQPLATWVVPPGQYFMMGDNRDNSADSRYWGFVPEANLVGKAVAI
EEECHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCEECCCCCCHHHHHHEEE
WMSFDKQEGEWPTGVRLSRIGGIH
EEEECCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 6311837; 9278503; 2202591; 1618816; 1546969; 8394311; 2551889; 9823901 [H]