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The map label for this gene is mcbR [H]

Identifier: 194736903

GI number: 194736903

Start: 1646832

End: 1647497

Strand: Reverse

Name: mcbR [H]

Synonym: SeSA_A1706

Alternate gene names: 194736903

Gene position: 1647497-1646832 (Counterclockwise)

Preceding gene: 194737426

Following gene: 194735817

Centisome position: 34.99

GC content: 46.85

Gene sequence:

>666_bases
ATGCCGGGTACGGAAAAAACACAACATATAAGCCTGACCACACAGGTTGAAAATCGGTTAAAGCATCAATTGTCTATTGG
CGCATTAAAACCAGGCGCAAGGCTTATCACTAAAAATATCGCTCAAGAGTTAGGCGTCAGTATTACGCCGGTGCGTGAGG
CTCTGTTAAGGCTGGTCTCTTCCAGTGCTTTGGCGGTGGCGCCAGCACAGGCGTTTATGGTGCCGGAGATCTCGCTGGAA
AGTTTGCTGGAAATTAATACGATTCGTACTGCCCTGGAGGAGATGGCGGTGGTCGCCGCTGCGGGTAAGATTACTCCGGA
TCGCGAGCAGACGCTGAATGCGCTATTAGATGAATTTCAACAGGCGCTGGAAAGTGGCGTTATGGAGAAAATTCTTTTGG
CCAACCGCGCATTCCGCTTTGAGATCTACCACTACGCGGATATGCCAACGCTGTATGCCATGATCGAGCAATTATGGGTA
CGCCTGGGGCCAAGCCTGCATTTTTTATATGATAATTTTAAACTTGATGATTATCAAAACGGCGTTAATCTGTACAGAAA
ATTGCTTAATGCGTTAGTGACTGGTGATAAAGAAGCCAGTCGCCACTGCCTACAGAACGTTTTGCAACAAAACGTAGCGA
CGATTAAAAATCAGTACTTCATGTAA

Upstream 100 bases:

>100_bases
TTGGCCGTTCAGGCCTGGATTATCTTTTTTACAATTAAGTTCCGAAACTTCTTCGGGACTCGATTGCGATGTTGTTCTGC
CAAGAGGTGAAGGAAGGAAA

Downstream 100 bases:

>100_bases
CTATGACTTATCAATAGCGGGGATTCCCCGCTATTGCCGGATATTTGCCGGATATTTATTCAAATTGCCATGCCAGATTA
ATCCCCACGCCATAGTTACG

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MPGTEKTQHISLTTQVENRLKHQLSIGALKPGARLITKNIAQELGVSITPVREALLRLVSSSALAVAPAQAFMVPEISLE
SLLEINTIRTALEEMAVVAAAGKITPDREQTLNALLDEFQQALESGVMEKILLANRAFRFEIYHYADMPTLYAMIEQLWV
RLGPSLHFLYDNFKLDDYQNGVNLYRKLLNALVTGDKEASRHCLQNVLQQNVATIKNQYFM

Sequences:

>Translated_221_residues
MPGTEKTQHISLTTQVENRLKHQLSIGALKPGARLITKNIAQELGVSITPVREALLRLVSSSALAVAPAQAFMVPEISLE
SLLEINTIRTALEEMAVVAAAGKITPDREQTLNALLDEFQQALESGVMEKILLANRAFRFEIYHYADMPTLYAMIEQLWV
RLGPSLHFLYDNFKLDDYQNGVNLYRKLLNALVTGDKEASRHCLQNVLQQNVATIKNQYFM
>Mature_220_residues
PGTEKTQHISLTTQVENRLKHQLSIGALKPGARLITKNIAQELGVSITPVREALLRLVSSSALAVAPAQAFMVPEISLES
LLEINTIRTALEEMAVVAAAGKITPDREQTLNALLDEFQQALESGVMEKILLANRAFRFEIYHYADMPTLYAMIEQLWVR
LGPSLHFLYDNFKLDDYQNGVNLYRKLLNALVTGDKEASRHCLQNVLQQNVATIKNQYFM

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=219, Percent_Identity=58.9041095890411, Blast_Score=255, Evalue=2e-69,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24796; Mature: 24665

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTEKTQHISLTTQVENRLKHQLSIGALKPGARLITKNIAQELGVSITPVREALLRLVS
CCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SSALAVAPAQAFMVPEISLESLLEINTIRTALEEMAVVAAAGKITPDREQTLNALLDEFQ
CCCHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
QALESGVMEKILLANRAFRFEIYHYADMPTLYAMIEQLWVRLGPSLHFLYDNFKLDDYQN
HHHHHHHHHHHHHHCCHHEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
GVNLYRKLLNALVTGDKEASRHCLQNVLQQNVATIKNQYFM
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PGTEKTQHISLTTQVENRLKHQLSIGALKPGARLITKNIAQELGVSITPVREALLRLVS
CCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SSALAVAPAQAFMVPEISLESLLEINTIRTALEEMAVVAAAGKITPDREQTLNALLDEFQ
CCCHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
QALESGVMEKILLANRAFRFEIYHYADMPTLYAMIEQLWVRLGPSLHFLYDNFKLDDYQN
HHHHHHHHHHHHHHCCHHEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
GVNLYRKLLNALVTGDKEASRHCLQNVLQQNVATIKNQYFM
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]