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The map label for this gene is yfgF [H]

Identifier: 194736785

GI number: 194736785

Start: 2621670

End: 2623883

Strand: Reverse

Name: yfgF [H]

Synonym: SeSA_A2741

Alternate gene names: 194736785

Gene position: 2623883-2621670 (Counterclockwise)

Preceding gene: 194734545

Following gene: 194734892

Centisome position: 55.72

GC content: 47.24

Gene sequence:

>2214_bases
ATGAAACTAAATAAAACTTATATAAATATCAGAGATAAATGGTGGGGACTACCGCTCATTCTCCCTTCGATTTTACTACC
TGTTTTAAGTAGCGCAAATACTTATGCGCTTACCAGCACTGGAAATGTGGTTCTTTTTTACCTGCCGTTAGCTTTTATGC
TTAGCCTGATGCTCTTTTTCGGCTGGGCAGCAATGCCGGGCATTGTGCTTGCTATCTTCTGGCGCAGATACCCGCAGACA
GGTCTGTATGAAACCCTTTCTGTGACAATGCACTTTATCATTACTATCGTGCTAAGTTGGGGCGGTTATCGTGTCTTTTC
CCCCCGGCGAAATAATGTTTCCCACGGCGACGCCCACCTGTTGTTTCAACGAATGTTCTGGCAGGTATTTTGTTCAGCAA
CATTATTTCTGGTCATCTACCAGTTTGCCGCTTTTGTTGGCATGTACGAAAGTAAAGCCAGCCTGATGGGGGTAATGCCC
TTTAATATCAACACGCTGATTAATTACCAGGCGTTACTGGTCGGTAACCTTATCGGCGTGCCATTGTGCTATTTTATTAT
TCGTACTCTCCGCAATCCGCTGCACCTGCGCGGGTATTATCAGCAATTAAAGCTACAGATTGATAGCAAGGTCACTAAAA
AAGAGATCGTTATTTGGCTGGCCGTATTGACTACACTAATGTTTATACTGTGTATGCCATTGACTGATAATAGTTCAATA
TTTAGTACCAACTATACGCTTTCGTTATTATTGCCGGTCATGCTGTGGGGCGCGATGCGCTATGGCTATAAGTTTATTTC
AATTATCTGGGCCGTCGTGCTAATTACGTCAATTCATTATTATCAGCGTTATATGCCCTGGTATTCAGGTTATGATACGC
AATTAGCGATTACCTCTTCGAGCTATCTGGTTTTTTCTTTTATTGTTAATTATATGTCTGTGCTGGCCACGCGCCAGCGC
GTAGTGAGTGGTCGCGCACGGCGGCTGGCCTATCTTGACCCGGTTGTACATCTTCCCAATTTACGTGCGTTAAACCGCGC
GCTCCAAAATGCGCCCTGGTCTACGATCTGTTTTTTACACGTGCCGGGTCTGGAGCTATTGGGTAAAAATTATGGCGTCA
TGCTACGCATCCAGTATAAGCAAAAGCTCTCTCACTGGATTACGCCGATGCTGGCCTCAAATGAGTGCGTCTACCAGATG
TCAGGGCACGACCTGGTGCTGCGTCTTAATACAGAGGCGCATCAGCAGCGTATTGAAGCGCTGGATAAACATATTAAGCA
GTTTCGGTTTATCTGGGACGGACTGCCGCTACAGCCGCCTGTCGGCGTGAGCTACTGCTGCGTGCGTTCTCCGGTTAGCC
ACCTTTATCTGCTGTTGGGCGAATTAAGCACTAGTTCCGATCTTTCGTTGACGACTAACGCGCCGGAGGATTTACAGCGG
CGCGGCGCAATGCACCTGCAGCGCGATTTGAAGGGCAGAATTGCGATGATGAATCGCCTGCAACAGGCGTTGGAGCACGA
TCATTTTTTCCTGATGGCGCAGCCTATTTTCGGTGTTCGTGGGGACGTTTACCATGAAATTTTACTGCGACTCAGGGATG
ACAACGGCGACGTTATCAACCCGGACAATTTTTTACCCGTGGCGCATGAGTTTGGTTTGTCTTCCGCTATCGATCTCTGG
GTGATTGAAAACACGCTGCGGTTTATGGCGGCCAGCCGGGAATATATGCCCGCGCATCGTTTTGCCATTAATCTGTCGCC
AACATCGGTATGCCGCGCCCGTTTTCCTGCAGAAGTTAAGCAGTTGCTTAAACAATATCAGGTTGAACCCTGGCAACTGG
TTTTTGAGGTAACGGAAAGCAACTCTCTCACCAATGCTGAGCAGGCGCAGTTGACGCTGGGACAGCTACAGCAGTTTGGC
TGTCAGATAGCGATTGATGATTTCGGTACAGGCTACGCCAGCTATGCGCGGCTGAAAAACGTTAACGCCGATATTCTTAA
AATTGACGGCAGTTTTATTCGCAATATAGTGTCAAACAGTCTCGATTATCAGATCGTGGCTTCTATTTGTCATTTAGCGC
GAATGAAAAAAATGCAGGTCGTCGCGGAGTATGTCGAGAGCGAAGAAATACGAAGCGTCGTGCTGTCGTTGGGGATCGAC
TATCTTCAGGGATATTTAATTGGCGAGCCGCAGCTATTATCTGAGATTCAGTAA

Upstream 100 bases:

>100_bases
ATGACTATCGTGTGATGTATATCGCATCATCAATTTAAAGTCAGGTGGGATGCCTGTCTGTTAACGCCCCTTTGGGATTG
GGGGGTTCTGCTGAGCATCT

Downstream 100 bases:

>100_bases
AGGCAGACGCCGCAGCGCGGCGTCTGATCCACAATTTACGCGGCGATCTCCGGCGAGCTTTCTTCCTCAATATTGAGACG
CCAGCCAGTTACCGCTTCCC

Product: inner membrane protein YfgF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 737; Mature: 737

Protein sequence:

>737_residues
MKLNKTYINIRDKWWGLPLILPSILLPVLSSANTYALTSTGNVVLFYLPLAFMLSLMLFFGWAAMPGIVLAIFWRRYPQT
GLYETLSVTMHFIITIVLSWGGYRVFSPRRNNVSHGDAHLLFQRMFWQVFCSATLFLVIYQFAAFVGMYESKASLMGVMP
FNINTLINYQALLVGNLIGVPLCYFIIRTLRNPLHLRGYYQQLKLQIDSKVTKKEIVIWLAVLTTLMFILCMPLTDNSSI
FSTNYTLSLLLPVMLWGAMRYGYKFISIIWAVVLITSIHYYQRYMPWYSGYDTQLAITSSSYLVFSFIVNYMSVLATRQR
VVSGRARRLAYLDPVVHLPNLRALNRALQNAPWSTICFLHVPGLELLGKNYGVMLRIQYKQKLSHWITPMLASNECVYQM
SGHDLVLRLNTEAHQQRIEALDKHIKQFRFIWDGLPLQPPVGVSYCCVRSPVSHLYLLLGELSTSSDLSLTTNAPEDLQR
RGAMHLQRDLKGRIAMMNRLQQALEHDHFFLMAQPIFGVRGDVYHEILLRLRDDNGDVINPDNFLPVAHEFGLSSAIDLW
VIENTLRFMAASREYMPAHRFAINLSPTSVCRARFPAEVKQLLKQYQVEPWQLVFEVTESNSLTNAEQAQLTLGQLQQFG
CQIAIDDFGTGYASYARLKNVNADILKIDGSFIRNIVSNSLDYQIVASICHLARMKKMQVVAEYVESEEIRSVVLSLGID
YLQGYLIGEPQLLSEIQ

Sequences:

>Translated_737_residues
MKLNKTYINIRDKWWGLPLILPSILLPVLSSANTYALTSTGNVVLFYLPLAFMLSLMLFFGWAAMPGIVLAIFWRRYPQT
GLYETLSVTMHFIITIVLSWGGYRVFSPRRNNVSHGDAHLLFQRMFWQVFCSATLFLVIYQFAAFVGMYESKASLMGVMP
FNINTLINYQALLVGNLIGVPLCYFIIRTLRNPLHLRGYYQQLKLQIDSKVTKKEIVIWLAVLTTLMFILCMPLTDNSSI
FSTNYTLSLLLPVMLWGAMRYGYKFISIIWAVVLITSIHYYQRYMPWYSGYDTQLAITSSSYLVFSFIVNYMSVLATRQR
VVSGRARRLAYLDPVVHLPNLRALNRALQNAPWSTICFLHVPGLELLGKNYGVMLRIQYKQKLSHWITPMLASNECVYQM
SGHDLVLRLNTEAHQQRIEALDKHIKQFRFIWDGLPLQPPVGVSYCCVRSPVSHLYLLLGELSTSSDLSLTTNAPEDLQR
RGAMHLQRDLKGRIAMMNRLQQALEHDHFFLMAQPIFGVRGDVYHEILLRLRDDNGDVINPDNFLPVAHEFGLSSAIDLW
VIENTLRFMAASREYMPAHRFAINLSPTSVCRARFPAEVKQLLKQYQVEPWQLVFEVTESNSLTNAEQAQLTLGQLQQFG
CQIAIDDFGTGYASYARLKNVNADILKIDGSFIRNIVSNSLDYQIVASICHLARMKKMQVVAEYVESEEIRSVVLSLGID
YLQGYLIGEPQLLSEIQ
>Mature_737_residues
MKLNKTYINIRDKWWGLPLILPSILLPVLSSANTYALTSTGNVVLFYLPLAFMLSLMLFFGWAAMPGIVLAIFWRRYPQT
GLYETLSVTMHFIITIVLSWGGYRVFSPRRNNVSHGDAHLLFQRMFWQVFCSATLFLVIYQFAAFVGMYESKASLMGVMP
FNINTLINYQALLVGNLIGVPLCYFIIRTLRNPLHLRGYYQQLKLQIDSKVTKKEIVIWLAVLTTLMFILCMPLTDNSSI
FSTNYTLSLLLPVMLWGAMRYGYKFISIIWAVVLITSIHYYQRYMPWYSGYDTQLAITSSSYLVFSFIVNYMSVLATRQR
VVSGRARRLAYLDPVVHLPNLRALNRALQNAPWSTICFLHVPGLELLGKNYGVMLRIQYKQKLSHWITPMLASNECVYQM
SGHDLVLRLNTEAHQQRIEALDKHIKQFRFIWDGLPLQPPVGVSYCCVRSPVSHLYLLLGELSTSSDLSLTTNAPEDLQR
RGAMHLQRDLKGRIAMMNRLQQALEHDHFFLMAQPIFGVRGDVYHEILLRLRDDNGDVINPDNFLPVAHEFGLSSAIDLW
VIENTLRFMAASREYMPAHRFAINLSPTSVCRARFPAEVKQLLKQYQVEPWQLVFEVTESNSLTNAEQAQLTLGQLQQFG
CQIAIDDFGTGYASYARLKNVNADILKIDGSFIRNIVSNSLDYQIVASICHLARMKKMQVVAEYVESEEIRSVVLSLGID
YLQGYLIGEPQLLSEIQ

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=733, Percent_Identity=73.5334242837653, Blast_Score=1155, Evalue=0.0,
Organism=Escherichia coli, GI87082096, Length=741, Percent_Identity=28.7449392712551, Blast_Score=313, Evalue=3e-86,
Organism=Escherichia coli, GI1790496, Length=243, Percent_Identity=34.5679012345679, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1787541, Length=435, Percent_Identity=26.8965517241379, Blast_Score=124, Evalue=3e-29,
Organism=Escherichia coli, GI87081743, Length=248, Percent_Identity=29.8387096774194, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786507, Length=231, Percent_Identity=31.6017316017316, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI226510982, Length=417, Percent_Identity=25.8992805755396, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1788502, Length=246, Percent_Identity=29.2682926829268, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI87081980, Length=249, Percent_Identity=29.3172690763052, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI87081921, Length=463, Percent_Identity=24.1900647948164, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1787055, Length=247, Percent_Identity=27.9352226720648, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI87081845, Length=234, Percent_Identity=28.6324786324786, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1789650, Length=246, Percent_Identity=22.7642276422764, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 84390; Mature: 84390

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNKTYINIRDKWWGLPLILPSILLPVLSSANTYALTSTGNVVLFYLPLAFMLSLMLFF
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHHHHHHH
GWAAMPGIVLAIFWRRYPQTGLYETLSVTMHFIITIVLSWGGYRVFSPRRNNVSHGDAHL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
LFQRMFWQVFCSATLFLVIYQFAAFVGMYESKASLMGVMPFNINTLINYQALLVGNLIGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCHHHHHHHHHHHHHHHHHH
PLCYFIIRTLRNPLHLRGYYQQLKLQIDSKVTKKEIVIWLAVLTTLMFILCMPLTDNSSI
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
FSTNYTLSLLLPVMLWGAMRYGYKFISIIWAVVLITSIHYYQRYMPWYSGYDTQLAITSS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEECC
SYLVFSFIVNYMSVLATRQRVVSGRARRLAYLDPVVHLPNLRALNRALQNAPWSTICFLH
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHCCCCHHHHHHHHHCCCCCEEEEEE
VPGLELLGKNYGVMLRIQYKQKLSHWITPMLASNECVYQMSGHDLVLRLNTEAHQQRIEA
CCCHHHHCCCCCEEEEEEHHHHHHHHHCHHHCCCCEEEEECCCEEEEEECCHHHHHHHHH
LDKHIKQFRFIWDGLPLQPPVGVSYCCVRSPVSHLYLLLGELSTSSDLSLTTNAPEDLQR
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHCHHHHHHHHHHCCCCCCCCEEECCCCHHHHH
RGAMHLQRDLKGRIAMMNRLQQALEHDHFFLMAQPIFGVRGDVYHEILLRLRDDNGDVIN
HCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEC
PDNFLPVAHEFGLSSAIDLWVIENTLRFMAASREYMPAHRFAINLSPTSVCRARFPAEVK
CCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCHHHH
QLLKQYQVEPWQLVFEVTESNSLTNAEQAQLTLGQLQQFGCQIAIDDFGTGYASYARLKN
HHHHHHCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHC
VNADILKIDGSFIRNIVSNSLDYQIVASICHLARMKKMQVVAEYVESEEIRSVVLSLGID
CCCCEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
YLQGYLIGEPQLLSEIQ
HHCCEECCCHHHHHCCC
>Mature Secondary Structure
MKLNKTYINIRDKWWGLPLILPSILLPVLSSANTYALTSTGNVVLFYLPLAFMLSLMLFF
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHHHHHHH
GWAAMPGIVLAIFWRRYPQTGLYETLSVTMHFIITIVLSWGGYRVFSPRRNNVSHGDAHL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
LFQRMFWQVFCSATLFLVIYQFAAFVGMYESKASLMGVMPFNINTLINYQALLVGNLIGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCHHHHHHHHHHHHHHHHHH
PLCYFIIRTLRNPLHLRGYYQQLKLQIDSKVTKKEIVIWLAVLTTLMFILCMPLTDNSSI
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
FSTNYTLSLLLPVMLWGAMRYGYKFISIIWAVVLITSIHYYQRYMPWYSGYDTQLAITSS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEECC
SYLVFSFIVNYMSVLATRQRVVSGRARRLAYLDPVVHLPNLRALNRALQNAPWSTICFLH
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHCCCCHHHHHHHHHCCCCCEEEEEE
VPGLELLGKNYGVMLRIQYKQKLSHWITPMLASNECVYQMSGHDLVLRLNTEAHQQRIEA
CCCHHHHCCCCCEEEEEEHHHHHHHHHCHHHCCCCEEEEECCCEEEEEECCHHHHHHHHH
LDKHIKQFRFIWDGLPLQPPVGVSYCCVRSPVSHLYLLLGELSTSSDLSLTTNAPEDLQR
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHCHHHHHHHHHHCCCCCCCCEEECCCCHHHHH
RGAMHLQRDLKGRIAMMNRLQQALEHDHFFLMAQPIFGVRGDVYHEILLRLRDDNGDVIN
HCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEC
PDNFLPVAHEFGLSSAIDLWVIENTLRFMAASREYMPAHRFAINLSPTSVCRARFPAEVK
CCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCHHHH
QLLKQYQVEPWQLVFEVTESNSLTNAEQAQLTLGQLQQFGCQIAIDDFGTGYASYARLKN
HHHHHHCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHC
VNADILKIDGSFIRNIVSNSLDYQIVASICHLARMKKMQVVAEYVESEEIRSVVLSLGID
CCCCEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
YLQGYLIGEPQLLSEIQ
HHCCEECCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]