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The map label for this gene is nuoM [H]

Identifier: 194736746

GI number: 194736746

Start: 2429154

End: 2430683

Strand: Reverse

Name: nuoM [H]

Synonym: SeSA_A2545

Alternate gene names: 194736746

Gene position: 2430683-2429154 (Counterclockwise)

Preceding gene: 194737362

Following gene: 194736101

Centisome position: 51.62

GC content: 56.08

Gene sequence:

>1530_bases
ATGTTACTACCCTGGCTAATATTGATTCCCTTTATCGGCGGCTTTCTGTGCTGGCAGACCGAACGCTTTGGCGTGAAGGT
GCCGCGCTGGATAGCGCTGATTACCATGGGGCTGACGCTGGCGCTCGGTCTGCAATTGTGGTTGCAGGGCGGCTACTCAC
TGACGCAATCCGCCGGTATTCCACAGTGGCAATCTGAATTTGTCCTGCCGTGGATCCCGCGTTTTGGCATCTCTATCCAT
CTGGCGCTCGACGGACTGTCGCTGCTAATGGTGGTGCTGACCGGCCTGCTCGGCGTTCTGGCGGTGCTTTGCTCCTGGCG
AGAAATCGAAAAATATCAGGGCTTCTTCCACCTGAACCTGATGTGGATCTTAGGCGGCGTTATCGGCGTGTTCCTGGCCA
TCGACATGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTGATAGCGCTGTGGGGGCATAAA
GCTTCTGACGGGAAAACGCGTATCACGGCGGCAACCAAGTTCTTCATTTATACCCAGGCGAGTGGTCTGGTGATGTTGAT
TGCCATCCTGGCGCTGGTCTTTGTGCACTACAACGCGACCGGCGTGTGGACTTTCAACTATGAAGAGCTGCTGAACACGC
CGATGTCCCACGGTGTGGAATATCTGCTGATGCTGGGCTTCTTCATCGCCTTCGCGGTGAAAATGCCGGTGGTGCCGCTG
CATGGCTGGCTGCCAGATGCGCACTCCCAGGCGCCGACCGCCGGTTCCGTCGACCTGGCCGGTATCTTGCTGAAAACCGC
CGCTTACGGTCTGCTGCGTTTCTCCCTGCCATTGTTCCCGAACGCGTCGGCGGAGTTTGCGCCGATCGCCATGTGGCTGG
GCGTTATCGGCATTTTCTACGGCGCGTGGATGGCCTTTACCCAATACGACATCAAGCGCCTGATTGCCTATACCTCCGTT
TCGCATATGGGCTTCGTGCTGATCGCTATCTACACCGGCAGCCAACTGGCGTACCAGGGCGCGGTGATTCAGATGATTGC
GCACGGTTTGTCCGCAGCCGGTCTGTTCATTCTGTGTGGCCAGTTGTACGAACGTCTGCATACGCGTGACATGCGTATGA
TGGGCGGCCTGTGGGGCAAGATGAAGTGGTTGCCTGCGCTCTCCATGTTCTTTGCGGTGGCGACGCTCGGGATGCCGGGC
ACCGGTAACTTTGTCGGCGAATTTATGATTCTGTTCGGCAGCTACCAGGTGGTGCCGGTGATTACCGTTATCTCCACCTT
TGGGTTGGTATTCGCCTCTGTATACTCGCTGGCGATGCTGCATCGCGCTTACTTTGGTAAAGCGAAGAGCCAGATTGCCA
GCCAGGAACTGCCAGGGATGTCGCTGCGCGAGCTGTTTATCATTCTGTTGCTGGTGGTGCTTCTGGTACTGCTTGGCTTC
TATCCGCAGCCGATTCTGGATACCTCGCATTCTGCGATGAGCAACATCCAGCAGTGGTTTGTTAATTCTGTTACTACTAC
AAGGCCGTAA

Upstream 100 bases:

>100_bases
GCGCTGCTGATGGTACTGCGTTGATCGTAAAGTGAATTTTATCCAAATTCGTTGAAAATCAGGCCTCTGTCAGAGGCCTT
AAAAAGGAATAAAAATCGCC

Downstream 100 bases:

>100_bases
ATCGCCATGACAATAACTCCACAACACCTGATTGCGCTGCTACCGCTGCTGATCGTCGGCTTGACGGTGGTGGTTGTGAT
GCTCTCCATTGCGTGGCGAC

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M; NUO13 [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALGLQLWLQGGYSLTQSAGIPQWQSEFVLPWIPRFGISIH
LALDGLSLLMVVLTGLLGVLAVLCSWREIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSHGVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFTQYDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERLHTRDMRMMGGLWGKMKWLPALSMFFAVATLGMPG
TGNFVGEFMILFGSYQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELPGMSLRELFIILLLVVLLVLLGF
YPQPILDTSHSAMSNIQQWFVNSVTTTRP

Sequences:

>Translated_509_residues
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALGLQLWLQGGYSLTQSAGIPQWQSEFVLPWIPRFGISIH
LALDGLSLLMVVLTGLLGVLAVLCSWREIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSHGVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFTQYDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERLHTRDMRMMGGLWGKMKWLPALSMFFAVATLGMPG
TGNFVGEFMILFGSYQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELPGMSLRELFIILLLVVLLVLLGF
YPQPILDTSHSAMSNIQQWFVNSVTTTRP
>Mature_509_residues
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALGLQLWLQGGYSLTQSAGIPQWQSEFVLPWIPRFGISIH
LALDGLSLLMVVLTGLLGVLAVLCSWREIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSHGVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFTQYDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERLHTRDMRMMGGLWGKMKWLPALSMFFAVATLGMPG
TGNFVGEFMILFGSYQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELPGMSLRELFIILLLVVLLVLLGF
YPQPILDTSHSAMSNIQQWFVNSVTTTRP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=368, Percent_Identity=32.3369565217391, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI251831117, Length=271, Percent_Identity=29.1512915129151, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788613, Length=509, Percent_Identity=97.0530451866405, Blast_Score=972, Evalue=0.0,
Organism=Escherichia coli, GI1788827, Length=451, Percent_Identity=24.6119733924612, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788831, Length=421, Percent_Identity=26.603325415677, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788614, Length=527, Percent_Identity=25.8064516129032, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI145693160, Length=442, Percent_Identity=25.1131221719457, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788829, Length=234, Percent_Identity=28.6324786324786, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 56666; Mature: 56666

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALGLQLWLQGGYSLTQSAGI
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
PQWQSEFVLPWIPRFGISIHLALDGLSLLMVVLTGLLGVLAVLCSWREIEKYQGFFHLNL
CCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
MWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECC
SGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSHGVEYLLMLGFFIAFAVKMPVVPL
CHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEC
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
GAWMAFTQYDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCG
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYERLHTRDMRMMGGLWGKMKWLPALSMFFAVATLGMPGTGNFVGEFMILFGSYQVVPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHH
ITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELPGMSLRELFIILLLVVLLVLLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
YPQPILDTSHSAMSNIQQWFVNSVTTTRP
CCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALGLQLWLQGGYSLTQSAGI
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
PQWQSEFVLPWIPRFGISIHLALDGLSLLMVVLTGLLGVLAVLCSWREIEKYQGFFHLNL
CCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
MWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECC
SGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSHGVEYLLMLGFFIAFAVKMPVVPL
CHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEC
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
GAWMAFTQYDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCG
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYERLHTRDMRMMGGLWGKMKWLPALSMFFAVATLGMPGTGNFVGEFMILFGSYQVVPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHH
ITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELPGMSLRELFIILLLVVLLVLLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
YPQPILDTSHSAMSNIQQWFVNSVTTTRP
CCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]