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The map label for this gene is focA [H]

Identifier: 194736658

GI number: 194736658

Start: 1065944

End: 1066759

Strand: Reverse

Name: focA [H]

Synonym: SeSA_A1087

Alternate gene names: 194736658

Gene position: 1066759-1065944 (Counterclockwise)

Preceding gene: 194736995

Following gene: 194737978

Centisome position: 22.65

GC content: 48.28

Gene sequence:

>816_bases
ATGGCCAAAGTGGCTGAAGAAGCAGGCGTCTATAAAGCAACGAAACATCCGCTTAAGACTTTCTATCTGGCGATTACCGC
CGGCGTATTCATTTCAATTGCCTTTGTTTTTTATATTACTGCGACAACCGGTACTGGCGCGATGCCTTATGGCATGGCGA
AACTTATCGGGGGTATCTGTTTCTCTTTGGGACTGATTCTCTGTGTTATTTGCGGCGCTGATCTTTTCACCTCTACTGTT
CTGATCGTTGTAGCTAAAGCTAGCGGTCGCATCACCTGGGGGCAACTGGCTAAAAACTGGTTAAACGTCTATTTTGGTAA
CCTGATTGGCGCTCTGCTTTTCGTGTTACTGATGTGGCTTTCCGGCGAGTATATGACTGCCAACGGTCAATGGGGACTTA
ACGTCCTGCAAACCGCCGACCACAAAATGCACCACACTTTTATTGAAGCTGTCTGCCTCGGTATTCTGGCGAACCTGATG
GTTTGTCTGGCCGTGTGGATGAGCTATTCAGGTCGTAGCCTGATGGACAAAGCGTTCATCATGGTACTGCCCGTCGCCAT
GTTTGTCGCCAGCGGTTTTGAGCACAGTATCGCAAACATGTTTATGATTCCTATGGGTATTGTAATACGCGATTTCGCTA
CACCGGAGTTTTGGACCGCAGTCGGTTCGTCTCCGGAAAGTTTTTCTCACCTGACCGTGATGAGCTTCATCACTGATAAC
CTGATTCCGGTCACGATCGGTAATATTATCGGCGGCGGTTTGTTGGTCGGGTTGACATACTGGGTCATTTACCTGCGTGG
TAACGAGCATCATTAA

Upstream 100 bases:

>100_bases
TCTCATCTATAATGCTTTGTTAGTATCTCGTCGCCGACTTAATAAAGAGAGAGTTAGTGTGAAAGCTGACAACCCTTTTG
ACCTTTTACTCCCTGCAGCA

Downstream 100 bases:

>100_bases
TCTGTTGATGCCCATTACACGCAGTAAATAAAAAATCCACTTAAGAAGGTAGGTGTTACATGTCCGAGCTTAATGAAAAG
TTAGCCACAGCCTGGGAAGG

Product: putative formate transporter FocA

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGAMPYGMAKLIGGICFSLGLILCVICGADLFTSTV
LIVVAKASGRITWGQLAKNWLNVYFGNLIGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKMHHTFIEAVCLGILANLM
VCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFATPEFWTAVGSSPESFSHLTVMSFITDN
LIPVTIGNIIGGGLLVGLTYWVIYLRGNEHH

Sequences:

>Translated_271_residues
MAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGAMPYGMAKLIGGICFSLGLILCVICGADLFTSTV
LIVVAKASGRITWGQLAKNWLNVYFGNLIGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKMHHTFIEAVCLGILANLM
VCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFATPEFWTAVGSSPESFSHLTVMSFITDN
LIPVTIGNIIGGGLLVGLTYWVIYLRGNEHH
>Mature_270_residues
AKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGAMPYGMAKLIGGICFSLGLILCVICGADLFTSTVL
IVVAKASGRITWGQLAKNWLNVYFGNLIGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKMHHTFIEAVCLGILANLMV
CLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFATPEFWTAVGSSPESFSHLTVMSFITDNL
IPVTIGNIIGGGLLVGLTYWVIYLRGNEHH

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=271, Percent_Identity=95.5719557195572, Blast_Score=533, Evalue=1e-153,
Organism=Escherichia coli, GI1788837, Length=265, Percent_Identity=50.9433962264151, Blast_Score=269, Evalue=1e-73,
Organism=Escherichia coli, GI87082249, Length=268, Percent_Identity=27.6119402985075, Blast_Score=75, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321779, Length=207, Percent_Identity=28.5024154589372, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 29471; Mature: 29340

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGAMPYGMAKLIGGIC
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
FSLGLILCVICGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFGNLIGALLFVLLMWL
HHHHHHHHHHHCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGEYMTANGQWGLNVLQTADHKMHHTFIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFI
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
MVLPVAMFVASGFEHSIANMFMIPMGIVIRDFATPEFWTAVGSSPESFSHLTVMSFITDN
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHCC
LIPVTIGNIIGGGLLVGLTYWVIYLRGNEHH
CHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
>Mature Secondary Structure 
AKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGAMPYGMAKLIGGIC
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
FSLGLILCVICGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFGNLIGALLFVLLMWL
HHHHHHHHHHHCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGEYMTANGQWGLNVLQTADHKMHHTFIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFI
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
MVLPVAMFVASGFEHSIANMFMIPMGIVIRDFATPEFWTAVGSSPESFSHLTVMSFITDN
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHCC
LIPVTIGNIIGGGLLVGLTYWVIYLRGNEHH
CHHHHHHHHHHHHHHHHHHHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]