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The map label for this gene is phsA [H]

Identifier: 194736523

GI number: 194736523

Start: 2118400

End: 2120676

Strand: Reverse

Name: phsA [H]

Synonym: SeSA_A2235

Alternate gene names: 194736523

Gene position: 2120676-2118400 (Counterclockwise)

Preceding gene: 194737371

Following gene: 194736046

Centisome position: 45.03

GC content: 55.38

Gene sequence:

>2277_bases
ATGAGCATTAGTCGTCGTTCTTTTTTGCAGGGGGTAGGCATCGGCTGCTCTGCCTGCGCGCTGGGCGCTTTTCCGCCCGG
GGCGCTGGCGCGCAACCCGATTGCCGGCATTAACGGTAAAACCACGCTAACGCCAAGCTTGTGCGAAATGTGTTCCTTTC
GTTGCCCTATTCAGGCGCAGGTAGTCAATAACAAGACCGTTTTTATCCAGGGCAATCCTTCTGCGCCGCAGCAGGGGACG
CGCATATGCGCCAGGGGCGGGAGCGGCGTCAGCCTGGTCAATGACCCGCAACGGATTGTAAAACCTATGAAACGCACCGG
GCCACGCGGCGACGGTGAATGGCAGGTGATTAGCTGGCAACAGGCTTACCAGGAAATCGCGGCGAAAATGAATGCCATCA
AAGTGCAGCATGGCCCCGAGACCGTGGCTTTCTCTTCCAAATCGGGCTCGCTCTCCAGCCATCTTTTCCATCTGGCTACG
GCCTTTGGTTCGCCTAATACCTTTACTCACGCCTCAACATGCCCTGCCGGGAAAGCCATTGCGGCAAAAGTGATGATGGG
CGGCGATCTGGCGATGGATATCGCTAACACGCGCTATCTGGTTTCGTTTGGCCACAATTTGTATGAAGGGATTGAAGTTG
CCGATACCCATGAGTTAATGACCGCGCAGGAGAAGGGGGCCAAAATGGTGAGCTTCGATCCGCGTTTGTCGATATTTTCC
AGCAAGGCGGATGAGTGGCACGCTATTCGTCCCGGGGGGGATTTAGCGGTTCTGCTGGCGATGTGCCACGTCATGATTGA
TGAACAACTCTACGATGCGTCTTTTGTTGAGCGTTATACCAGCGGATTTGAACAGTTAGCACAGGCGGTAAAAGAGACGA
CGCCGGAATGGGCCGCCGCGCAGGCCGATGTCCCGGCCGACGTTATTGTCCGGGTGACACGCGAACTGGCCGCCTGCGCG
CCTCACGCTATTGTCAGTCCTGGTCATCGCGCGACGTTCTCGCAGGAAGAGATCGATATGCGGCGTATGATTTTTACGCT
TAATGTACTGCTCGGTAATATTGAGCGTGAAGGCGGGCTATATCAGAAAAAAAACGCGTCGGTTTACAACAAACTGGCCG
GAGAAAAGGTCGCGCCAACGCTGGCGAAACTCAACATTAAAAATATGCCGAAACCGACGGCGCAACGAATCGATTTGGTC
GCACCGCAGTTTAAATATATCGCCGCTGGCGGCGGCGTGGTGCAAAGCATTATTGACGCGGTGTTAACCCAGAAGCCTTA
CCCGGTAAAGGCGTGGATTATGTCGCGGCATAATCCTTTCCAGACCGTCACCTGTCGTTCGGACCTGGTAAAAACCGTTG
AGCAACTGGATCTGGTGGTTAGCTGCGATGTCTATTTGAGCGAGAGCGCGGCGTATGCCGACTATCTGCTGCCGGAATGC
ACTTATCTCGAACGGGACGAAGAGGTATCGGATATGTCGGGACTGCACCCGGCTTACGCTCTGCGCCAACAGGTCGTAGA
GCCGATTGGCGAGGCGCGTCCGAGTTGGCAAATCTGGAAAGAACTTGGCGAGCAGTTGGGATTAGGGCAGTACTATCCGT
GGCAGGATATGCAGACGCGCCAACTCTATCAGTTGAACGGCGACCATGCCTTAGCGAAGGAACTGCGACAGAAAGGGTAT
CTCGAATGGGGCGTTCCGCTGCTATTACGCGAACCAGAATCCGTTCGCCAGTTTACGGCGCGTTACCCCGGCGCTATCGC
GACGGACAGCGACAACACCTATGGCGAACAGCTTCGCTTCAAATCGCCCTCCGGCAAAATCGAACTTTATTCCGCAACCC
TGGAGGAATTGCTCCCAGGCTACGGCGTTCCGCGCGTTCGTGACTTTGCGCTTAAAAAAGAGAATGAGCTTTACTTCATT
CAGGGCAAAGTGGCCGTGCATACCAATGGCGCGACGCAGTACGTACCTTTACTCAGCGAGCTAATGTGGGATAACGCGGT
CTGGGTCCATCCGCAAACGGCGCAAGAAAAAGGCATTAAGACCGGCGATGAGATCTGGCTGGAAAATGCTACGGGTAAAG
AGAAAGGTAAGGCGCTGGTGACGCCCGGAATCCGCCCGGACACGCTTTTTGTCTATATGGGATTTGGCGCTAAAGCTGGG
GCCAAAACGGCGGCGACGACACACGGTATCCACTGCGGAAATTTACTGCCGCACGTGACGAGTCCGGTATCCGGTACGGT
AGTGCATACCGCAGGCGTGACGCTGAGCCGGGCATGA

Upstream 100 bases:

>100_bases
CGTGTTCATTTTAACAACTATTTCAAATTGTGATTGAAGCCGACATTTCATAATACAAATTAAATATAATCTTCAGCTAT
ATCTAATAACAGGAGGTTAT

Downstream 100 bases:

>100_bases
GGGAGGAGGGAACCATGAATCATTTAACGAATCAGTACGTCATGCTGCATGATGAAAAACGTTGTATCGGCTGCCAGGCG
TGTACCGTTGCCTGCAAAGT

Product: thiosulfate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MSISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQVVNNKTVFIQGNPSAPQQGT
RICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQQAYQEIAAKMNAIKVQHGPETVAFSSKSGSLSSHLFHLAT
AFGSPNTFTHASTCPAGKAIAAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFS
SKADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTPEWAAAQADVPADVIVRVTRELAACA
PHAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGLYQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLV
APQFKYIAAGGGVVQSIIDAVLTQKPYPVKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPEC
TYLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTRQLYQLNGDHALAKELRQKGY
LEWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRFKSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFI
QGKVAVHTNGATQYVPLLSELMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAG
AKTAATTHGIHCGNLLPHVTSPVSGTVVHTAGVTLSRA

Sequences:

>Translated_758_residues
MSISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQVVNNKTVFIQGNPSAPQQGT
RICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQQAYQEIAAKMNAIKVQHGPETVAFSSKSGSLSSHLFHLAT
AFGSPNTFTHASTCPAGKAIAAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFS
SKADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTPEWAAAQADVPADVIVRVTRELAACA
PHAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGLYQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLV
APQFKYIAAGGGVVQSIIDAVLTQKPYPVKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPEC
TYLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTRQLYQLNGDHALAKELRQKGY
LEWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRFKSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFI
QGKVAVHTNGATQYVPLLSELMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAG
AKTAATTHGIHCGNLLPHVTSPVSGTVVHTAGVTLSRA
>Mature_757_residues
SISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQVVNNKTVFIQGNPSAPQQGTR
ICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQQAYQEIAAKMNAIKVQHGPETVAFSSKSGSLSSHLFHLATA
FGSPNTFTHASTCPAGKAIAAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFSS
KADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTPEWAAAQADVPADVIVRVTRELAACAP
HAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGLYQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLVA
PQFKYIAAGGGVVQSIIDAVLTQKPYPVKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPECT
YLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTRQLYQLNGDHALAKELRQKGYL
EWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRFKSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFIQ
GKVAVHTNGATQYVPLLSELMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAGA
KTAATTHGIHCGNLLPHVTSPVSGTVVHTAGVTLSRA

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787870, Length=835, Percent_Identity=26.7065868263473, Blast_Score=200, Evalue=4e-52,
Organism=Escherichia coli, GI87081797, Length=791, Percent_Identity=25.5372945638432, Blast_Score=185, Evalue=1e-47,
Organism=Escherichia coli, GI171474008, Length=828, Percent_Identity=25.1207729468599, Blast_Score=176, Evalue=6e-45,
Organism=Escherichia coli, GI3868721, Length=730, Percent_Identity=24.5205479452055, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787231, Length=717, Percent_Identity=22.1757322175732, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI87081994, Length=795, Percent_Identity=22.3899371069182, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1788534, Length=822, Percent_Identity=20.6812652068127, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 82841; Mature: 82709

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1 ; PS00490 MOLYBDOPTERIN_PROK_2 ; PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQ
CCCCHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHCCCCCEEEE
VVNNKTVFIQGNPSAPQQGTRICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQ
EECCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHH
QAYQEIAAKMNAIKVQHGPETVAFSSKSGSLSSHLFHLATAFGSPNTFTHASTCPAGKAI
HHHHHHHHHHHHEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
AAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFS
HHHEEECCCEEEECCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHCCEEEECCCCHHHHC
SKADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTPEWAAA
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
QADVPADVIVRVTRELAACAPHAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGL
CCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
YQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLVAPQFKYIAAGGGVVQSIIDA
CCCCCCHHHHHHCCCHHCCHHHHCCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHH
VLTQKPYPVKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPEC
HHCCCCCCEEEEEECCCCCCEEEECHHHHHHHHHHHCEEEEECEEECCCHHHHHHHCCCC
TYLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTR
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCH
QLYQLNGDHALAKELRQKGYLEWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRF
HHEEECCCHHHHHHHHHCCCEECCCCEEEECCHHHHHHHHHCCCCEECCCCCCCCCCEEE
KSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFIQGKVAVHTNGATQYVPLLSE
ECCCCCEEEHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEEEEEEECCCHHHHHHHHH
LMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAG
HHHCCEEEECCCHHHHCCCCCCCCEEEECCCCCCCCCEEECCCCCCCEEEEEECCCCCCC
AKTAATTHGIHCGNLLPHVTSPVSGTVVHTAGVTLSRA
CCCCHHCCCCCCCCCCCCCCCCCCCEEEEECCEEEECC
>Mature Secondary Structure 
SISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQ
CCCHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHCCCCCEEEE
VVNNKTVFIQGNPSAPQQGTRICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQ
EECCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHH
QAYQEIAAKMNAIKVQHGPETVAFSSKSGSLSSHLFHLATAFGSPNTFTHASTCPAGKAI
HHHHHHHHHHHHEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
AAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFS
HHHEEECCCEEEECCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHCCEEEECCCCHHHHC
SKADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTPEWAAA
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
QADVPADVIVRVTRELAACAPHAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGL
CCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
YQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLVAPQFKYIAAGGGVVQSIIDA
CCCCCCHHHHHHCCCHHCCHHHHCCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHH
VLTQKPYPVKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPEC
HHCCCCCCEEEEEECCCCCCEEEECHHHHHHHHHHHCEEEEECEEECCCHHHHHHHCCCC
TYLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTR
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCH
QLYQLNGDHALAKELRQKGYLEWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRF
HHEEECCCHHHHHHHHHCCCEECCCCEEEECCHHHHHHHHHCCCCEECCCCCCCCCCEEE
KSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFIQGKVAVHTNGATQYVPLLSE
ECCCCCEEEHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEEEEEEECCCHHHHHHHHH
LMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAG
HHHCCEEEECCCHHHHCCCCCCCCEEEECCCCCCCCCEEECCCCCCCEEEEEECCCCCCC
AKTAATTHGIHCGNLLPHVTSPVSGTVVHTAGVTLSRA
CCCCHHCCCCCCCCCCCCCCCCCCCEEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]