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The map label for this gene is yeiT [H]

Identifier: 194736184

GI number: 194736184

Start: 2301859

End: 2303100

Strand: Direct

Name: yeiT [H]

Synonym: SeSA_A2425

Alternate gene names: 194736184

Gene position: 2301859-2303100 (Clockwise)

Preceding gene: 194737953

Following gene: 194736993

Centisome position: 48.88

GC content: 50.64

Gene sequence:

>1242_bases
ATGCCGCAACAAAATTATCTGGATGAACTCACGCCTGGTTTTACGCCATTACTGGCAATAAAAGAGGCTTCCCGTTGTTT
ATTATGTCACGATGCGCCCTGTAGTCAGGCTTGCCCGGCGCAAACCGATCCGGGGAAATTTATTCGTTCTATTTACTTTC
GCAATTTTAAAGGCGCAGCGGAAACTATCCGGGAAAATAACGCGCTTGGCGCCGTCTGCGCCAGAGTCTGTCCGACGGAA
AAATTATGCCAGCGCGGATGTACACGTTCCGGAATAGATAAACCTATTGATATTGCACGCCTGCAACGTTTTATTACCGA
TTTTGAACAGCAGACTGCCATGCAGATTTATCAGCCCGGGAGTAAAACACGCGGAAAAGTCGCGATTATTGGCGCGGGTC
CGGCAGGTTTGCAGGCAAGCGTGACGCTCACGCATTTAGGCTATGACGTCACCATTTACGAAAAACAGCCGCAGCCCGGC
GGATGGCTGCGTCACGGCATCCCGGCATTTCGTCTGCCGCAAAGCGTACTCGATCAGGAAATTGCCCGCATTGTAGAAAT
GGGCGTCAATATTAAATGTAATTGTGACGTGGGCGGGTCGCTATCGCTTGCGCAACTCAAAGCCGAATATCGCGCCGTGC
TGATGACCATCGGGATGTCCTATGGATCTGATTTACCGTTATTTGAGCAAGCCAGTCACGTAGAGATTGCCGTTGATTTT
TTACAGCGCGCCCGTCAGGCCGACGGCGATATTAGCGTTCCGCGTAGCGCATTAATTATTGGCGGCGGCGACGTAGCGAT
GGATGTCGCCAGTACGTTAAAAATTCTCGGCTGTCCTTCCGTCACCTGCGTGGCGCGGGAAGAATTAGCCGAATTCCCCG
CCAGCGAAAAAGAATTTACCAGCACGCAGGCATTAGGCGTATCGATTATTGATGGTTTTACGCCTGTCGCCGTCAGCGGA
AATAAAGTGACCTTCCACCATGTACGCCACTCAGGAGAACTGACGCTGGAAGCGGAAAATATTATTTTGGCCGTGGGTCA
GCACGCGCGACTGGATACCTTTGCGGAGATAAAAGCGCAGCATAATATTATCGACACGCACAATTATCAAACCGACGACC
CGGCGATCTTTGCCGCTGGCGATATTGTTAAAGGCGATAAGACCGTCGTTTATGCGGTAAAAACCGGAAAAGAAGCCGCT
CAGGCTATTCATCATTATTTAGAGGAGGCCTGCTCATGTTAA

Upstream 100 bases:

>100_bases
ATACCCTCTTTTCAATGCTTAGGCTGGCGGTAGATTTTAGATCATTAAAACGCGTTATTTGCGTTTCGCCTTAATTTATT
CAAGCATTAAGGAAGATTAT

Downstream 100 bases:

>100_bases
CAAAAGATTTGTCTGTTACCTTTTGCGGCGTTAAGTTTCCCAATCCGTTTTGTCTTTCTTCTTCTCCGGTAGGCAATTGT
TATGAGATGTGCGCCAAAGC

Product: putative oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MPQQNYLDELTPGFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQRGCTRSGIDKPIDIARLQRFITDFEQQTAMQIYQPGSKTRGKVAIIGAGPAGLQASVTLTHLGYDVTIYEKQPQPG
GWLRHGIPAFRLPQSVLDQEIARIVEMGVNIKCNCDVGGSLSLAQLKAEYRAVLMTIGMSYGSDLPLFEQASHVEIAVDF
LQRARQADGDISVPRSALIIGGGDVAMDVASTLKILGCPSVTCVAREELAEFPASEKEFTSTQALGVSIIDGFTPVAVSG
NKVTFHHVRHSGELTLEAENIILAVGQHARLDTFAEIKAQHNIIDTHNYQTDDPAIFAAGDIVKGDKTVVYAVKTGKEAA
QAIHHYLEEACSC

Sequences:

>Translated_413_residues
MPQQNYLDELTPGFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQRGCTRSGIDKPIDIARLQRFITDFEQQTAMQIYQPGSKTRGKVAIIGAGPAGLQASVTLTHLGYDVTIYEKQPQPG
GWLRHGIPAFRLPQSVLDQEIARIVEMGVNIKCNCDVGGSLSLAQLKAEYRAVLMTIGMSYGSDLPLFEQASHVEIAVDF
LQRARQADGDISVPRSALIIGGGDVAMDVASTLKILGCPSVTCVAREELAEFPASEKEFTSTQALGVSIIDGFTPVAVSG
NKVTFHHVRHSGELTLEAENIILAVGQHARLDTFAEIKAQHNIIDTHNYQTDDPAIFAAGDIVKGDKTVVYAVKTGKEAA
QAIHHYLEEACSC
>Mature_412_residues
PQQNYLDELTPGFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEK
LCQRGCTRSGIDKPIDIARLQRFITDFEQQTAMQIYQPGSKTRGKVAIIGAGPAGLQASVTLTHLGYDVTIYEKQPQPGG
WLRHGIPAFRLPQSVLDQEIARIVEMGVNIKCNCDVGGSLSLAQLKAEYRAVLMTIGMSYGSDLPLFEQASHVEIAVDFL
QRARQADGDISVPRSALIIGGGDVAMDVASTLKILGCPSVTCVAREELAEFPASEKEFTSTQALGVSIIDGFTPVAVSGN
KVTFHHVRHSGELTLEAENIILAVGQHARLDTFAEIKAQHNIIDTHNYQTDDPAIFAAGDIVKGDKTVVYAVKTGKEAAQ
AIHHYLEEACSC

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=437, Percent_Identity=30.4347826086957, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI237757300, Length=90, Percent_Identity=46.6666666666667, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1788468, Length=413, Percent_Identity=83.2929782082325, Blast_Score=712, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=450, Percent_Identity=31.3333333333333, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1789606, Length=442, Percent_Identity=30.7692307692308, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87082180, Length=451, Percent_Identity=30.5986696230599, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789243, Length=399, Percent_Identity=27.8195488721804, Blast_Score=128, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71984108, Length=443, Percent_Identity=31.6027088036117, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17570289, Length=446, Percent_Identity=27.3542600896861, Blast_Score=145, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6320030, Length=463, Percent_Identity=23.7580993520518, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24640763, Length=434, Percent_Identity=29.4930875576037, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI18858217, Length=434, Percent_Identity=29.4930875576037, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24665547, Length=444, Percent_Identity=28.1531531531532, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24665543, Length=444, Percent_Identity=28.1531531531532, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28574881, Length=444, Percent_Identity=28.1531531531532, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24665539, Length=444, Percent_Identity=28.1531531531532, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 44655; Mature: 44524

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQQNYLDELTPGFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCCHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHH
ETIRENNALGAVCARVCPTEKLCQRGCTRSGIDKPIDIARLQRFITDFEQQTAMQIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SKTRGKVAIIGAGPAGLQASVTLTHLGYDVTIYEKQPQPGGWLRHGIPAFRLPQSVLDQE
CCCCCEEEEEECCCCCCEEEEEEEEECEEEEEEECCCCCCCHHHHCCCHHCCCHHHHHHH
IARIVEMGVNIKCNCDVGGSLSLAQLKAEYRAVLMTIGMSYGSDLPLFEQASHVEIAVDF
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHH
LQRARQADGDISVPRSALIIGGGDVAMDVASTLKILGCPSVTCVAREELAEFPASEKEFT
HHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHH
STQALGVSIIDGFTPVAVSGNKVTFHHVRHSGELTLEAENIILAVGQHARLDTFAEIKAQ
HHHHCCEEEECCCCCEEECCCEEEEEEEECCCCEEEEECEEEEEECCCCCCHHHHHHHHH
HNIIDTHNYQTDDPAIFAAGDIVKGDKTVVYAVKTGKEAAQAIHHYLEEACSC
CCEEECCCCCCCCCEEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PQQNYLDELTPGFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHH
ETIRENNALGAVCARVCPTEKLCQRGCTRSGIDKPIDIARLQRFITDFEQQTAMQIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SKTRGKVAIIGAGPAGLQASVTLTHLGYDVTIYEKQPQPGGWLRHGIPAFRLPQSVLDQE
CCCCCEEEEEECCCCCCEEEEEEEEECEEEEEEECCCCCCCHHHHCCCHHCCCHHHHHHH
IARIVEMGVNIKCNCDVGGSLSLAQLKAEYRAVLMTIGMSYGSDLPLFEQASHVEIAVDF
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHH
LQRARQADGDISVPRSALIIGGGDVAMDVASTLKILGCPSVTCVAREELAEFPASEKEFT
HHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHH
STQALGVSIIDGFTPVAVSGNKVTFHHVRHSGELTLEAENIILAVGQHARLDTFAEIKAQ
HHHHCCEEEECCCCCEEECCCEEEEEEEECCCCEEEEECEEEEEECCCCCCHHHHHHHHH
HNIIDTHNYQTDDPAIFAAGDIVKGDKTVVYAVKTGKEAAQAIHHYLEEACSC
CCEEECCCCCCCCCEEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]