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The map label for this gene is lasT [H]

Identifier: 194736168

GI number: 194736168

Start: 4708440

End: 4709051

Strand: Direct

Name: lasT [H]

Synonym: SeSA_A4850

Alternate gene names: 194736168

Gene position: 4708440-4709051 (Clockwise)

Preceding gene: 194735853

Following gene: NA

Centisome position: 99.99

GC content: 52.12

Gene sequence:

>612_bases
ATGGGATTTACTGACCTGCGTATTGTCGACAGCCAGGCGCACCTGGAGCCCGCCACCCGTTGGGTCGCACATGGATCTGG
AGATATTATTGATAATATTGAGGTTTTCCACACCCTTGCCGACGCGCTCCACGATGTGGATTTTACCGTCGCGACGACAG
CCCGCAGCCGGGCAAAATTTCATTACTACGCTTCGCCCGCTGAACTGGTTCCCTTATTACAGGAAAAATCACGCTGGATG
CGTCATGCCGCGCTGGTTTTTGGCCGTGAGGATTCCGGTTTGACCAATGACGAGCTGGCGCTGGCGGATGTATTGACCGG
CGTGCCGATGGCGGCGGATTACCCTTCGCTCAATCTGGGACAGGCAGTCATGGTGTATTGCTATCAATTAGCAGGTTTAA
TGCAACAGACCACAGAATCCGTTGATATTGCTGATGAATCGCAGTTACAGGCGTTACGCGCGCGCCTTTTACGCCTGCTA
ACGACTCTGGAGGCGGCCGATGACCACAAATTAACCGACTGGCTACAACAGCGAATTGGCCTGCTGGGACAGCGAGATAC
GGTAATGTTGCACCGTTTGGTCCATGATATTGAAAAAAAACTAACAAAATAA

Upstream 100 bases:

>100_bases
TAAATTAGTCAAAAAGAGTAATGAAATGCGTGTAACAATCGTTCTTGTCGCTCCCGCCAGAGCGGAAAATATCGGCGCAG
CCGCCCGGGCTATGAAGACC

Downstream 100 bases:

>100_bases
CGTGCTGTAATTTTTAAAATAATAAGAGATTACGTCTGGTTGCAAGAGATCATAACAGGGGGAATTGGTTGAAACTAAAT
ATATCGCCAGCAGCACATGA

Product: putative RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MGFTDLRIVDSQAHLEPATRWVAHGSGDIIDNIEVFHTLADALHDVDFTVATTARSRAKFHYYASPAELVPLLQEKSRWM
RHAALVFGREDSGLTNDELALADVLTGVPMAADYPSLNLGQAVMVYCYQLAGLMQQTTESVDIADESQLQALRARLLRLL
TTLEAADDHKLTDWLQQRIGLLGQRDTVMLHRLVHDIEKKLTK

Sequences:

>Translated_203_residues
MGFTDLRIVDSQAHLEPATRWVAHGSGDIIDNIEVFHTLADALHDVDFTVATTARSRAKFHYYASPAELVPLLQEKSRWM
RHAALVFGREDSGLTNDELALADVLTGVPMAADYPSLNLGQAVMVYCYQLAGLMQQTTESVDIADESQLQALRARLLRLL
TTLEAADDHKLTDWLQQRIGLLGQRDTVMLHRLVHDIEKKLTK
>Mature_202_residues
GFTDLRIVDSQAHLEPATRWVAHGSGDIIDNIEVFHTLADALHDVDFTVATTARSRAKFHYYASPAELVPLLQEKSRWMR
HAALVFGREDSGLTNDELALADVLTGVPMAADYPSLNLGQAVMVYCYQLAGLMQQTTESVDIADESQLQALRARLLRLLT
TLEAADDHKLTDWLQQRIGLLGQRDTVMLHRLVHDIEKKLTK

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=203, Percent_Identity=81.7733990147783, Blast_Score=344, Evalue=2e-96,
Organism=Escherichia coli, GI1788881, Length=130, Percent_Identity=34.6153846153846, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 22733; Mature: 22602

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFTDLRIVDSQAHLEPATRWVAHGSGDIIDNIEVFHTLADALHDVDFTVATTARSRAKF
CCCCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCE
HYYASPAELVPLLQEKSRWMRHAALVFGREDSGLTNDELALADVLTGVPMAADYPSLNLG
EEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHH
QAVMVYCYQLAGLMQQTTESVDIADESQLQALRARLLRLLTTLEAADDHKLTDWLQQRIG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LLGQRDTVMLHRLVHDIEKKLTK
HCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GFTDLRIVDSQAHLEPATRWVAHGSGDIIDNIEVFHTLADALHDVDFTVATTARSRAKF
CCCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCE
HYYASPAELVPLLQEKSRWMRHAALVFGREDSGLTNDELALADVLTGVPMAADYPSLNLG
EEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHH
QAVMVYCYQLAGLMQQTTESVDIADESQLQALRARLLRLLTTLEAADDHKLTDWLQQRIG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LLGQRDTVMLHRLVHDIEKKLTK
HCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8022271 [H]