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The map label for this gene is queF [H]

Identifier: 194735727

GI number: 194735727

Start: 3022157

End: 3023005

Strand: Direct

Name: queF [H]

Synonym: SeSA_A3128

Alternate gene names: 194735727

Gene position: 3022157-3023005 (Clockwise)

Preceding gene: 194737545

Following gene: 194734921

Centisome position: 64.18

GC content: 53.47

Gene sequence:

>849_bases
ATGTCTTCTTATGAAAACCATCAGGCTCTCGATGGCCTGACTCTCGGAAAATCAACGGATTACCGGGACAATTATGATGC
CAGCCTGCTGCAGGGCGTACCGCGCAGTTTGAATCGCGACCCGCTCGGATTAACAGCCGATAATTTGCCGTTCCACGGCG
CGGATATCTGGACGTTGTATGAACTTTCCTGGCTTAACAGCCGTGGTCTTCCGCAGGTCGCCGTCGGGCATGTCGAACTG
GATTACACCAGCGTTAATTTAATCGAATCCAAAAGCTTTAAACTGTATCTGAACAGCTTTAACCAGACGCGCTTTGACAC
CTGGGAGACGGTGCGCCAGACGCTGGAGCGCGATCTGCGCGCCTGCGCGCAGGGCAACGTTAGCGTGAGGCTTCATCGTC
TTGATGAGCTGGAAGGACAGCCTGTCGCCCATTTCCACGGCACCTGTATTGACGATCAGGATATCAGTATCGACAACTAC
CAGTTTACTACCGACTATCTGCAACATGCGGTCAGCGGCGAGAAGCAAGTAGAGGAGACGCTGGTCAGTCATCTGCTGAA
ATCCAACTGCCTGATCACCCACCAACCGGACTGGGGCTCAATACAAATTCAGTATCGCGGGCGCAAAATCGACAGGGAAA
AACTGCTGCGTTATCTGGTCTCCTTCCGTCACCATAATGAATTTCACGAGCAGTGCGTAGAGCGTATTTTTAATGACATT
CTGCGCTTCTGTCAGCCGGAAACGTTAAGCGTTTATGCGCGCTATACCCGCCGCGGCGGGCTGGACATTAATCCCTGGCG
CAGCAATGCCGATTTTGTGCCCGCCACGGGCAGGCTGGCGCGTCAGTAA

Upstream 100 bases:

>100_bases
GCTTTCAATGCCTGCGCTGTCAGTTCGTCCACCAGTTTCTCTCTTCATAAAAGCCAGGATACACTTGGCGCATAGTGTAT
CTGGTTTATGACGGTGAAAC

Downstream 100 bases:

>100_bases
TCTTTTTTGTCAATTTTGCGTGCCGGATTGCGCATGTAAGGTTGTGAAAGGTCATCAGCCAGGGCTATTGTAATCAACAG
GGAATGGCGTGTTGCGTCCC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSSYENHQALDGLTLGKSTDYRDNYDASLLQGVPRSLNRDPLGLTADNLPFHGADIWTLYELSWLNSRGLPQVAVGHVEL
DYTSVNLIESKSFKLYLNSFNQTRFDTWETVRQTLERDLRACAQGNVSVRLHRLDELEGQPVAHFHGTCIDDQDISIDNY
QFTTDYLQHAVSGEKQVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRKIDREKLLRYLVSFRHHNEFHEQCVERIFNDI
LRFCQPETLSVYARYTRRGGLDINPWRSNADFVPATGRLARQ

Sequences:

>Translated_282_residues
MSSYENHQALDGLTLGKSTDYRDNYDASLLQGVPRSLNRDPLGLTADNLPFHGADIWTLYELSWLNSRGLPQVAVGHVEL
DYTSVNLIESKSFKLYLNSFNQTRFDTWETVRQTLERDLRACAQGNVSVRLHRLDELEGQPVAHFHGTCIDDQDISIDNY
QFTTDYLQHAVSGEKQVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRKIDREKLLRYLVSFRHHNEFHEQCVERIFNDI
LRFCQPETLSVYARYTRRGGLDINPWRSNADFVPATGRLARQ
>Mature_281_residues
SSYENHQALDGLTLGKSTDYRDNYDASLLQGVPRSLNRDPLGLTADNLPFHGADIWTLYELSWLNSRGLPQVAVGHVELD
YTSVNLIESKSFKLYLNSFNQTRFDTWETVRQTLERDLRACAQGNVSVRLHRLDELEGQPVAHFHGTCIDDQDISIDNYQ
FTTDYLQHAVSGEKQVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRKIDREKLLRYLVSFRHHNEFHEQCVERIFNDIL
RFCQPETLSVYARYTRRGGLDINPWRSNADFVPATGRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=282, Percent_Identity=87.5886524822695, Blast_Score=527, Evalue=1e-151,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 32644; Mature: 32513

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYENHQALDGLTLGKSTDYRDNYDASLLQGVPRSLNRDPLGLTADNLPFHGADIWTLY
CCCCCCCHHHCCEECCCCCCCCCCCCHHHHHHCHHHCCCCCCCCCCCCCCCCCCCEEEEE
ELSWLNSRGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQTRFDTWETVRQTLERDLR
EEECCCCCCCCCEEEEEEEEEEEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
ACAQGNVSVRLHRLDELEGQPVAHFHGTCIDDQDISIDNYQFTTDYLQHAVSGEKQVEET
HHHCCCCEEEEEEHHCCCCCCCHHCCCCCCCCCCCCCCCEEHHHHHHHHHHCCHHHHHHH
LVSHLLKSNCLITHQPDWGSIQIQYRGRKIDREKLLRYLVSFRHHNEFHEQCVERIFNDI
HHHHHHHCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LRFCQPETLSVYARYTRRGGLDINPWRSNADFVPATGRLARQ
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
SSYENHQALDGLTLGKSTDYRDNYDASLLQGVPRSLNRDPLGLTADNLPFHGADIWTLY
CCCCCCHHHCCEECCCCCCCCCCCCHHHHHHCHHHCCCCCCCCCCCCCCCCCCCEEEEE
ELSWLNSRGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQTRFDTWETVRQTLERDLR
EEECCCCCCCCCEEEEEEEEEEEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
ACAQGNVSVRLHRLDELEGQPVAHFHGTCIDDQDISIDNYQFTTDYLQHAVSGEKQVEET
HHHCCCCEEEEEEHHCCCCCCCHHCCCCCCCCCCCCCCCEEHHHHHHHHHHCCHHHHHHH
LVSHLLKSNCLITHQPDWGSIQIQYRGRKIDREKLLRYLVSFRHHNEFHEQCVERIFNDI
HHHHHHHCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LRFCQPETLSVYARYTRRGGLDINPWRSNADFVPATGRLARQ
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA