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The map label for this gene is proP [H]

Identifier: 194735683

GI number: 194735683

Start: 4421558

End: 4423060

Strand: Direct

Name: proP [H]

Synonym: SeSA_A4545

Alternate gene names: 194735683

Gene position: 4421558-4423060 (Clockwise)

Preceding gene: 194734663

Following gene: 194734705

Centisome position: 93.89

GC content: 53.03

Gene sequence:

>1503_bases
ATGCTGAAAAGGAAAAAAATAAAACCGATTACACTGGGCGATGTGACCATCATTGATGACGGTAAACTTCGCAAAGCGAT
TACCGCCGCCTCGCTGGGCAACGCGATGGAGTGGTTTGATTTTGGTGTTTATGGATTTGTTGCCTACGCGTTGGGTAAAG
TCTTTTTCCCCGGCGCCGATCCCAGCGTCCAGATGATTGCCGCGCTGGCCACGTTTTCCGTTCCCTTCCTGATTCGTCCG
CTCGGCGGGTTATTCTTTGGTATGCTCGGCGATAAATACGGGCGCCAGAAGATCCTGGCGATAACGATTGTGATTATGTC
GATCAGTACCTTCTGTATCGGGTTAATTCCCTCTTACGCGACGATCGGTATCTGGGCGCCAATACTGTTGTTGCTATGTA
AAATAGCGCAGGGCTTCTCGGTTGGCGGGGAATATACCGGCGCGTCGATCTTTGTCGCGGAATATTCGCCGGATCGTAAA
CGCGGATTTATGGGAAGCTGGTTGGATTTTGGTTCCATCGCCGGGTTCGTGCTGGGCGCGGGCGTGGTGGTCTTAATCTC
AACGATTGTCGGCGAGGAGAATTTCCTGGAGTGGGGCTGGCGTATTCCGTTCTTTATCGCTCTGCCATTGGGAATTATTG
GTCTCTACTTACGCCATGCGCTGGAGGAGACGCCAGCGTTTCAGCAGCACGTGGATAAACTGGAGCAGGGCGACCGCGAA
GGGTTGCAGGATGGGCCGAAAGTCTCCTTTAAAGAGATTGCCACCAAACACTGGCGGAGCCTGTTGTCATGTATCGGTCT
GGTGATTGCCACCAACGTGACCTACTACATGCTGCTCACCTACATGCCGAGCTACCTGTCGCATAACCTGCACTATTCTG
AAGATCACGGCGTGTTGATTATCATCGCCATTATGATCGGGATGCTGTTTGTGCAGCCGGTGATGGGGCTGCTGAGCGAC
CGTTTCGGTCGACGTCCATTTGTGATTATGGGCAGCATTGCGCTGTTCGCGCTGGCGATCCCGGCCTTCATCCTGATTAA
CAGTAACGTTATTGGCCTGATTTTTGCCGGTTTGTTGATGCTGGCGGTGATTCTGAACTGCTTTACCGGGGTGATGGCCT
CGACATTGCCGGCGATGTTTCCGACGCATATTCGTTACAGCGCGCTGGCGGCGGCTTTTAATATCTCTGTATTGATTGCC
GGTCTGACGCCAACGCTGGCGGCCTGGCTGGTGGAAAGCTCGCAGGATCTGATGATGCCGGCGTATTATTTGATGGTCAT
CGCGGTGATAGGCTTGGTTACCGGTATTTCCATGAAAGAGACGGCCAATCGTCCGCTAAAAGGCGCAACGCCAGCGGCGT
CGGACATCCAGGAAGCGAAGGAAATTCTGGGCGAGCATTACGATAATATTGAGCAGAAAATCGACGACATCGATCAGGAA
ATCGCGGAGCTGCAGGTCAAGCGTTCGCGTCTGGTACAGCAACATCCGCGCATCGATGAATAA

Upstream 100 bases:

>100_bases
ATAGCGTTCGCGCCCCTCCCTCCGCTCGACGGCGACGCTGGCGCGGTATGCCAGTGCCCGCCGTATATAGCGCTACAGGG
CTTAGCCTATGAGGACAGCT

Downstream 100 bases:

>100_bases
ATTTCGCGCTTAAGGTTCGCTTAATCTCTCGCGGGCATACTCTCCTCCATACCTTTGGAGGAGAGCGTCATGAAAAGCTA
TATTTATAAAAGTTTGACGA

Product: proline/glycine betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MLKRKKIKPITLGDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPGADPSVQMIAALATFSVPFLIRP
LGGLFFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYATIGIWAPILLLLCKIAQGFSVGGEYTGASIFVAEYSPDRK
RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLEWGWRIPFFIALPLGIIGLYLRHALEETPAFQQHVDKLEQGDRE
GLQDGPKVSFKEIATKHWRSLLSCIGLVIATNVTYYMLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSD
RFGRRPFVIMGSIALFALAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAAFNISVLIA
GLTPTLAAWLVESSQDLMMPAYYLMVIAVIGLVTGISMKETANRPLKGATPAASDIQEAKEILGEHYDNIEQKIDDIDQE
IAELQVKRSRLVQQHPRIDE

Sequences:

>Translated_500_residues
MLKRKKIKPITLGDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPGADPSVQMIAALATFSVPFLIRP
LGGLFFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYATIGIWAPILLLLCKIAQGFSVGGEYTGASIFVAEYSPDRK
RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLEWGWRIPFFIALPLGIIGLYLRHALEETPAFQQHVDKLEQGDRE
GLQDGPKVSFKEIATKHWRSLLSCIGLVIATNVTYYMLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSD
RFGRRPFVIMGSIALFALAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAAFNISVLIA
GLTPTLAAWLVESSQDLMMPAYYLMVIAVIGLVTGISMKETANRPLKGATPAASDIQEAKEILGEHYDNIEQKIDDIDQE
IAELQVKRSRLVQQHPRIDE
>Mature_500_residues
MLKRKKIKPITLGDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPGADPSVQMIAALATFSVPFLIRP
LGGLFFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYATIGIWAPILLLLCKIAQGFSVGGEYTGASIFVAEYSPDRK
RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLEWGWRIPFFIALPLGIIGLYLRHALEETPAFQQHVDKLEQGDRE
GLQDGPKVSFKEIATKHWRSLLSCIGLVIATNVTYYMLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSD
RFGRRPFVIMGSIALFALAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAAFNISVLIA
GLTPTLAAWLVESSQDLMMPAYYLMVIAVIGLVTGISMKETANRPLKGATPAASDIQEAKEILGEHYDNIEQKIDDIDQE
IAELQVKRSRLVQQHPRIDE

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=500, Percent_Identity=94.8, Blast_Score=979, Evalue=0.0,
Organism=Escherichia coli, GI1788292, Length=443, Percent_Identity=32.0541760722348, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1789941, Length=431, Percent_Identity=35.0348027842227, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1788942, Length=436, Percent_Identity=30.2752293577982, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87082231, Length=487, Percent_Identity=23.4086242299795, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI87082404, Length=390, Percent_Identity=25.6410256410256, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323512, Length=459, Percent_Identity=25.0544662309368, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 54756; Mature: 54756

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRKKIKPITLGDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPGAD
CCCCCCCCCEEECCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PSVQMIAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TIGIWAPILLLLCKIAQGFSVGGEYTGASIFVAEYSPDRKRGFMGSWLDFGSIAGFVLGA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHCCCCCCHHHHHHHHHHHHHH
GVVVLISTIVGEENFLEWGWRIPFFIALPLGIIGLYLRHALEETPAFQQHVDKLEQGDRE
HHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GLQDGPKVSFKEIATKHWRSLLSCIGLVIATNVTYYMLLTYMPSYLSHNLHYSEDHGVLI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IIAIMIGMLFVQPVMGLLSDRFGRRPFVIMGSIALFALAIPAFILINSNVIGLIFAGLLM
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAVILNCFTGVMASTLPAMFPTHIRYSALAAAFNISVLIAGLTPTLAAWLVESSQDLMMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHCCCHHHHH
AYYLMVIAVIGLVTGISMKETANRPLKGATPAASDIQEAKEILGEHYDNIEQKIDDIDQE
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAELQVKRSRLVQQHPRIDE
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLKRKKIKPITLGDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPGAD
CCCCCCCCCEEECCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PSVQMIAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TIGIWAPILLLLCKIAQGFSVGGEYTGASIFVAEYSPDRKRGFMGSWLDFGSIAGFVLGA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHCCCCCCHHHHHHHHHHHHHH
GVVVLISTIVGEENFLEWGWRIPFFIALPLGIIGLYLRHALEETPAFQQHVDKLEQGDRE
HHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GLQDGPKVSFKEIATKHWRSLLSCIGLVIATNVTYYMLLTYMPSYLSHNLHYSEDHGVLI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IIAIMIGMLFVQPVMGLLSDRFGRRPFVIMGSIALFALAIPAFILINSNVIGLIFAGLLM
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAVILNCFTGVMASTLPAMFPTHIRYSALAAAFNISVLIAGLTPTLAAWLVESSQDLMMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHCCCHHHHH
AYYLMVIAVIGLVTGISMKETANRPLKGATPAASDIQEAKEILGEHYDNIEQKIDDIDQE
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAELQVKRSRLVQQHPRIDE
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]