Click here to switch to the map view.

The map label for this gene is yfiN [H]

Identifier: 194734312

GI number: 194734312

Start: 2766241

End: 2767380

Strand: Direct

Name: yfiN [H]

Synonym: SeSA_A2867

Alternate gene names: 194734312

Gene position: 2766241-2767380 (Clockwise)

Preceding gene: 194734937

Following gene: 194736792

Centisome position: 58.74

GC content: 50.79

Gene sequence:

>1140_bases
ATGACATTGATCTGGCTATTAATTTGCGTTGCGTCTGTCCTTACGCTCAAACAGTATGCGCAAAAGAATCTCGATTTGAC
CGCCGCCACAATGGCCCATAGCCTTGAAGCGGCACTGGTATTTTCCGATAACACGGCCGCAGCGGAAACGCTCGCCACAC
TGGGACGCCAGGGACAATTTTCAGCGGCGGAGGTCCGCGATAAAAATGGCCGTATTATCGCCTCATGGCGCTATGATGCG
CGAGCCGCAGACGATAAGCTCATTGGCTTAATTAGCCACTGGCTTTTTCCATTGCCGGTATCGCAACCCGTCTGGCACAA
CGGCAGGGCCATCGGCGAAGTACGGCTTGTCGCCCGCGACAGCCTTATTGGTCATTTTATCTGGCTATCGCTGGCAGTGC
TGACAGGATGTATTCTGCTGGCATCCGGCATTGCCCTGCTGCTCACGCGTTATTTACACAATGGCGTTGTGGATGCGCTG
CAAAATATTACTGAAGTTGTACACGACGTTCGCACTAACCGAAATTTTTCACGCCGGGTACCTGATGAGCGTATTGCGGA
ATTTCACCTGTTTGCGCAGGATTTCAATAGCCTTCTGGATGAGATGGAAGAATGGCAGCTACGGCTTCAGGCTAAAAATG
CCCAGTTACTACGTACCGCGCTGCACGATCCGCTGACGGGGCTTGCCAATCGCGCAGCATTTCGCAGCTGTATTAACGCG
CTGATGAAGGACAATTCCGCTCGTAGCAGTTCGGCATTGTTATTTCTGGATGGCGATAACTTTAAATATATTAATGATAC
CTGGGGACATGCGGCAGGCGACCGCGTACTTATAGAGGTTGCCAAAAGATTAGCGGAATTCGGTGGTAGCCGTTATCAGA
CTTACCGACTCGGCGGCGATGAATTTGCGATGGTGCTTTACGATGTACATTCGGAATATGAAGTACAACGTATTTGCGCA
GCGCTATCCCAGGCGTTTAATCGACCTTTTGAACTGCATAACGGCCAGCGGATAACGATGACCATGAGTATTGGCTTTGC
GCTGACATGGGAACATGCCACTGCCGAAAAACTACAAGAACTGGCCGATCGAAATATGTATCAGGCTAAACACCGGCGTG
CGGAACGCTCTCTAAACTAA

Upstream 100 bases:

>100_bases
CTTGCACGGAATCAAAAGCATGAATAAGGAATTTTCTCTGTCCAGACCAACATTTAAACGCACACTTCGGCGGATTAGTA
TAATCAGCGTGCTGCTTACG

Downstream 100 bases:

>100_bases
GGAACGGGCCTGGCCGTTTCTGACTCAGCGTTGAGACCACAGACCCAGCCTAATCATGCAACCGTCTATGGCGTGGAGCA
AACCCATAAAAAAGGGGCCA

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MTLIWLLICVASVLTLKQYAQKNLDLTAATMAHSLEAALVFSDNTAAAETLATLGRQGQFSAAEVRDKNGRIIASWRYDA
RAADDKLIGLISHWLFPLPVSQPVWHNGRAIGEVRLVARDSLIGHFIWLSLAVLTGCILLASGIALLLTRYLHNGVVDAL
QNITEVVHDVRTNRNFSRRVPDERIAEFHLFAQDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSCINA
LMKDNSARSSSALLFLDGDNFKYINDTWGHAAGDRVLIEVAKRLAEFGGSRYQTYRLGGDEFAMVLYDVHSEYEVQRICA
ALSQAFNRPFELHNGQRITMTMSIGFALTWEHATAEKLQELADRNMYQAKHRRAERSLN

Sequences:

>Translated_379_residues
MTLIWLLICVASVLTLKQYAQKNLDLTAATMAHSLEAALVFSDNTAAAETLATLGRQGQFSAAEVRDKNGRIIASWRYDA
RAADDKLIGLISHWLFPLPVSQPVWHNGRAIGEVRLVARDSLIGHFIWLSLAVLTGCILLASGIALLLTRYLHNGVVDAL
QNITEVVHDVRTNRNFSRRVPDERIAEFHLFAQDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSCINA
LMKDNSARSSSALLFLDGDNFKYINDTWGHAAGDRVLIEVAKRLAEFGGSRYQTYRLGGDEFAMVLYDVHSEYEVQRICA
ALSQAFNRPFELHNGQRITMTMSIGFALTWEHATAEKLQELADRNMYQAKHRRAERSLN
>Mature_378_residues
TLIWLLICVASVLTLKQYAQKNLDLTAATMAHSLEAALVFSDNTAAAETLATLGRQGQFSAAEVRDKNGRIIASWRYDAR
AADDKLIGLISHWLFPLPVSQPVWHNGRAIGEVRLVARDSLIGHFIWLSLAVLTGCILLASGIALLLTRYLHNGVVDALQ
NITEVVHDVRTNRNFSRRVPDERIAEFHLFAQDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSCINAL
MKDNSARSSSALLFLDGDNFKYINDTWGHAAGDRVLIEVAKRLAEFGGSRYQTYRLGGDEFAMVLYDVHSEYEVQRICAA
LSQAFNRPFELHNGQRITMTMSIGFALTWEHATAEKLQELADRNMYQAKHRRAERSLN

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=376, Percent_Identity=76.8617021276596, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI145693134, Length=152, Percent_Identity=34.8684210526316, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1788381, Length=113, Percent_Identity=42.4778761061947, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787056, Length=165, Percent_Identity=32.1212121212121, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787262, Length=153, Percent_Identity=30.0653594771242, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787816, Length=193, Percent_Identity=26.4248704663212, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=168, Percent_Identity=30.3571428571429, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786584, Length=158, Percent_Identity=29.1139240506329, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=30.5882352941176, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787541, Length=150, Percent_Identity=30, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87082007, Length=175, Percent_Identity=30.2857142857143, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42637; Mature: 42506

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLIWLLICVASVLTLKQYAQKNLDLTAATMAHSLEAALVFSDNTAAAETLATLGRQGQF
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCCCCC
SAAEVRDKNGRIIASWRYDARAADDKLIGLISHWLFPLPVSQPVWHNGRAIGEVRLVARD
CHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEHHHH
SLIGHFIWLSLAVLTGCILLASGIALLLTRYLHNGVVDALQNITEVVHDVRTNRNFSRRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
PDERIAEFHLFAQDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSCINA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
LMKDNSARSSSALLFLDGDNFKYINDTWGHAAGDRVLIEVAKRLAEFGGSRYQTYRLGGD
HHCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCC
EFAMVLYDVHSEYEVQRICAALSQAFNRPFELHNGQRITMTMSIGFALTWEHATAEKLQE
CEEEEEEHHCCHHHHHHHHHHHHHHHCCCEECCCCCEEEEEEEECEEEEECHHHHHHHHH
LADRNMYQAKHRRAERSLN
HHHCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TLIWLLICVASVLTLKQYAQKNLDLTAATMAHSLEAALVFSDNTAAAETLATLGRQGQF
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCCCCC
SAAEVRDKNGRIIASWRYDARAADDKLIGLISHWLFPLPVSQPVWHNGRAIGEVRLVARD
CHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEHHHH
SLIGHFIWLSLAVLTGCILLASGIALLLTRYLHNGVVDALQNITEVVHDVRTNRNFSRRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
PDERIAEFHLFAQDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSCINA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
LMKDNSARSSSALLFLDGDNFKYINDTWGHAAGDRVLIEVAKRLAEFGGSRYQTYRLGGD
HHCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCC
EFAMVLYDVHSEYEVQRICAALSQAFNRPFELHNGQRITMTMSIGFALTWEHATAEKLQE
CEEEEEEHHCCHHHHHHHHHHHHHHHCCCEECCCCCEEEEEEEECEEEEECHHHHHHHHH
LADRNMYQAKHRRAERSLN
HHHCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]