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The map label for this gene is menE [H]

Identifier: 194734141

GI number: 194734141

Start: 2417575

End: 2418942

Strand: Reverse

Name: menE [H]

Synonym: SeSA_A2533

Alternate gene names: 194734141

Gene position: 2418942-2417575 (Counterclockwise)

Preceding gene: 194734201

Following gene: 194737998

Centisome position: 51.37

GC content: 60.38

Gene sequence:

>1368_bases
ATGACATTCACGGACTGGCCGTGGCGCCACTGGCGTCAGGTTCGCAGTCAGGCGCCCGCTCTGCGCCTGAACGATGAAGT
GCTAAGCTGGCGCGCCCTCTGTGAGCGTATTGATGCGCTGGCGGATGGGTTTGCCGCGCAGGGCGTACGGGAGGGCGACG
GTGTCCTGCTGCGCGCCGGGAATCAGCCGCGCACTCTGTTGGCATGGCTTGCGCTGATGCAGTGCGGCGCGCGGGTTCTG
CCCGTCAACCCGCAGTTGCCCCAGACGTTGCTGGAGGCGCTGTTACCGGAGTTAACGCTGCGTTTTGCGCTGACACTGGA
AGGCGAAAACGTATTTTCCGGGTTAACCGCGTTGCAGATACAAAAGAGTACAGCGGCGTATGCCGTGGCCTGGCAACCGC
AGCGTCTGGTTTCTATGACGCTGACATCGGGTTCCACAGGATTGCCAAAAGCGGCTGTACATACGTGCCAGGCGCATCTT
GCCAGCGCGCAGGGGGTGCTTTCGCTAATGCCGTTTGGCCCTCAGGATGACTGGCTGCTATCGCTGCCGCTGTTTCATGT
ATCCGGGCAGGGTATTATGTGGCGTTGGCTCTTCGCCGGGGCGCGCATGACGGTACGCGATAAGCAACCGCTGGAGCAGA
TGCTGGCAGGGTGTACTCATGCTTCGCTGGTGCCGACGCAGCTATGGCGGTTGCTGGCGAATCGGGCGGCGGTGACGCTA
AAAGCCGTTTTGCTCGGCGGCGCGGTGATTCCGGTTGAACTCACCGACCAGGCCAGCAAACAAGGGATTCGCTGCTGGTG
CGGGTATGGGCTCACGGAGTTCGCGTCTACGGTATGCGCCAAAGAGGCCGATGGTTCGGATGATGTGGGCGCGCCGCTCC
CCGGCAGAGAGATCAGAATCGTCGATAATGAAGTCTGGCTGCGTGCCGCCAGTATGGCAGAAGGTTACTGGCGTGATGGC
AAGCTGATCCCGCTGGTTAATGACGAGGGCTGGTTTGCCACGCGCGATCGCGGCGAGCTGAACCACGGCAGGCTAACCAT
CGCCGGGCGGCTGGACAACCTGTTCTTTAGCGGCGGCGAAGGCATTCAGCCGGAGGAAGTCGAACGCGTTATTAACGCGC
ATCCGCTGGTGCAACAAGCCTTCGTCGTGCCGGTAGAAGATAAAGAGTTTGGTCATCGTCCCGTTGCGGTTGTGGAGTAT
GCATCGCAGGCCGGAGACGTGAACCTCGCTGAATGGGTCCGTGATAAGCTGGCGCGTTTTCAGCAGCCGGTTCGCTGGCT
AACGCTGCCGTCAGAACTGAAAAACGGAGGGATTAAAATATCTCGCCGTGCGCTCCAGCAGTGGGTGTGTGAGAGCTGTA
AAAATTAA

Upstream 100 bases:

>100_bases
CTGGACTGGATACCTTGCATCTGATGCAGGCGCAACAGGTTCGCCCCTGGCCTGGTAGCGCGTTGCCTTGTCTGAAGCGT
GATGAGCTGGAACGACTGTT

Downstream 100 bases:

>100_bases
TGTTAGGTTAATATACAACCATTCCATCGCTATTGCCGTTTTGTTTATCCGTTGATGTGTAACGTGTTATGAACAATCAG
CGTGAAACGGGGGCGCTATG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTFTDWPWRHWRQVRSQAPALRLNDEVLSWRALCERIDALADGFAAQGVREGDGVLLRAGNQPRTLLAWLALMQCGARVL
PVNPQLPQTLLEALLPELTLRFALTLEGENVFSGLTALQIQKSTAAYAVAWQPQRLVSMTLTSGSTGLPKAAVHTCQAHL
ASAQGVLSLMPFGPQDDWLLSLPLFHVSGQGIMWRWLFAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLANRAAVTL
KAVLLGGAVIPVELTDQASKQGIRCWCGYGLTEFASTVCAKEADGSDDVGAPLPGREIRIVDNEVWLRAASMAEGYWRDG
KLIPLVNDEGWFATRDRGELNHGRLTIAGRLDNLFFSGGEGIQPEEVERVINAHPLVQQAFVVPVEDKEFGHRPVAVVEY
ASQAGDVNLAEWVRDKLARFQQPVRWLTLPSELKNGGIKISRRALQQWVCESCKN

Sequences:

>Translated_455_residues
MTFTDWPWRHWRQVRSQAPALRLNDEVLSWRALCERIDALADGFAAQGVREGDGVLLRAGNQPRTLLAWLALMQCGARVL
PVNPQLPQTLLEALLPELTLRFALTLEGENVFSGLTALQIQKSTAAYAVAWQPQRLVSMTLTSGSTGLPKAAVHTCQAHL
ASAQGVLSLMPFGPQDDWLLSLPLFHVSGQGIMWRWLFAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLANRAAVTL
KAVLLGGAVIPVELTDQASKQGIRCWCGYGLTEFASTVCAKEADGSDDVGAPLPGREIRIVDNEVWLRAASMAEGYWRDG
KLIPLVNDEGWFATRDRGELNHGRLTIAGRLDNLFFSGGEGIQPEEVERVINAHPLVQQAFVVPVEDKEFGHRPVAVVEY
ASQAGDVNLAEWVRDKLARFQQPVRWLTLPSELKNGGIKISRRALQQWVCESCKN
>Mature_454_residues
TFTDWPWRHWRQVRSQAPALRLNDEVLSWRALCERIDALADGFAAQGVREGDGVLLRAGNQPRTLLAWLALMQCGARVLP
VNPQLPQTLLEALLPELTLRFALTLEGENVFSGLTALQIQKSTAAYAVAWQPQRLVSMTLTSGSTGLPKAAVHTCQAHLA
SAQGVLSLMPFGPQDDWLLSLPLFHVSGQGIMWRWLFAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLANRAAVTLK
AVLLGGAVIPVELTDQASKQGIRCWCGYGLTEFASTVCAKEADGSDDVGAPLPGREIRIVDNEVWLRAASMAEGYWRDGK
LIPLVNDEGWFATRDRGELNHGRLTIAGRLDNLFFSGGEGIQPEEVERVINAHPLVQQAFVVPVEDKEFGHRPVAVVEYA
SQAGDVNLAEWVRDKLARFQQPVRWLTLPSELKNGGIKISRRALQQWVCESCKN

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=449, Percent_Identity=79.5100222717149, Blast_Score=705, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=495, Percent_Identity=24.2424242424242, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786801, Length=416, Percent_Identity=25.2403846153846, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788107, Length=517, Percent_Identity=24.7582205029014, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32563687, Length=431, Percent_Identity=25.754060324826, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17558820, Length=460, Percent_Identity=25.8695652173913, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17557194, Length=496, Percent_Identity=22.7822580645161, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17538037, Length=491, Percent_Identity=24.2362525458248, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=370, Percent_Identity=26.7567567567568, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17560308, Length=297, Percent_Identity=26.2626262626263, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24581924, Length=511, Percent_Identity=23.679060665362, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50272; Mature: 50141

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFTDWPWRHWRQVRSQAPALRLNDEVLSWRALCERIDALADGFAAQGVREGDGVLLRAG
CCCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEECC
NQPRTLLAWLALMQCGARVLPVNPQLPQTLLEALLPELTLRFALTLEGENVFSGLTALQI
CCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHEEEEEEEECCCHHHHCCHHEEE
QKSTAAYAVAWQPQRLVSMTLTSGSTGLPKAAVHTCQAHLASAQGVLSLMPFGPQDDWLL
ECCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCCCEE
SLPLFHVSGQGIMWRWLFAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLANRAAVTL
EEEEEEECCCCEEEEEEHHCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KAVLLGGAVIPVELTDQASKQGIRCWCGYGLTEFASTVCAKEADGSDDVGAPLPGREIRI
HHHHHCCEEEEEEECCCHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
VDNEVWLRAASMAEGYWRDGKLIPLVNDEGWFATRDRGELNHGRLTIAGRLDNLFFSGGE
ECCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCCCEEEEEEEECCCEECCCC
GIQPEEVERVINAHPLVQQAFVVPVEDKEFGHRPVAVVEYASQAGDVNLAEWVRDKLARF
CCCHHHHHHHHHCCCHHHHHEEEEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
QQPVRWLTLPSELKNGGIKISRRALQQWVCESCKN
HCCCEEEECHHHHCCCCEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TFTDWPWRHWRQVRSQAPALRLNDEVLSWRALCERIDALADGFAAQGVREGDGVLLRAG
CCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEECC
NQPRTLLAWLALMQCGARVLPVNPQLPQTLLEALLPELTLRFALTLEGENVFSGLTALQI
CCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHEEEEEEEECCCHHHHCCHHEEE
QKSTAAYAVAWQPQRLVSMTLTSGSTGLPKAAVHTCQAHLASAQGVLSLMPFGPQDDWLL
ECCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCCCEE
SLPLFHVSGQGIMWRWLFAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLANRAAVTL
EEEEEEECCCCEEEEEEHHCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KAVLLGGAVIPVELTDQASKQGIRCWCGYGLTEFASTVCAKEADGSDDVGAPLPGREIRI
HHHHHCCEEEEEEECCCHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
VDNEVWLRAASMAEGYWRDGKLIPLVNDEGWFATRDRGELNHGRLTIAGRLDNLFFSGGE
ECCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCCCEEEEEEEECCCEECCCC
GIQPEEVERVINAHPLVQQAFVVPVEDKEFGHRPVAVVEYASQAGDVNLAEWVRDKLARF
CCCHHHHHHHHHCCCHHHHHEEEEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
QQPVRWLTLPSELKNGGIKISRRALQQWVCESCKN
HCCCEEEECHHHHCCCCEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]