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The map label for this gene is hupB [H]

Identifier: 194734103

GI number: 194734103

Start: 1579455

End: 1581257

Strand: Reverse

Name: hupB [H]

Synonym: SeSA_A1642

Alternate gene names: 194734103

Gene position: 1581257-1579455 (Counterclockwise)

Preceding gene: 194737982

Following gene: 194734434

Centisome position: 33.58

GC content: 52.3

Gene sequence:

>1803_bases
ATGGCATATCCTTATCAGACTCAGGGTTTTACGCTGGACAATAGCGGACGACGAATTGTGGTCGATCCGGTTACTCGCAT
CGAAGGGCATATGCGCTGCGAAGTGAATATCGACAGTAATAATGTCATTACTAACGCCGTGTCGACCGGCACCATGTGGC
GCGGCCTTGAGGTTATCCTGAAAGGTCGCGATCCGCGAGACGCCTGGGCATTCGTTGAACGCATTTGCGGCGTCTGCACC
GGTACACACGCTCTGACCTCAATCCGTGCCGTAGAAAATGCGCTGGGAATCGCGATTCCGGACAACGCAAACTGTATCCG
TAATATGATGCAGGCTACGCTACACGTTCACGATCACCTGGTGCATTTTTATCATCTGCACGCGCTGGACTGGGTTGATG
TGGTTGCCGCCCTGAAAGCCGACCCGCATCAGACCTCCGCCATCGCGCAAAGCCTTTCCGCATGGCCGCTATCCTCGCCG
GGATATTTCCGCGATTTGCAAAATCGACTGAAGCGGTTTATCGAGTCCGGTCAACTTGGCCCTTTCCGTAATGGCTACTG
GGGACATCCGGCGATGAAACTTCCGCCGGAAGCCAATTTGCTGGCGGTCGCCCACTATCTTGAAGCACTCGATTTTCAAA
AAGAGATCGTAAAAATTCACACCGTTTTCGGTGGTAAAAACCCGCATCCTAACTGGCTGGTGGGCGGCGTTCCTTGCGCC
ATCAACCTTGACGAAACGGGCGCGGTCGGCGCAGTGAATATGGAACGCCTGAACCTTGTCAGTTCCATCATCCAAAAAGC
TCGCCAGTTCTGCGAACAGGTTTATCTTCCGGACGTCTTGCTGATTGCCAGTTATTACAAAGACTGGGCAAAAATCGGCG
GCGGGTTATCGAGCATGAACCTGCTGGCTTATGGCGAGTTTCCGGATAACCCTAACGATTATTCCGCCAGTAATCTTCTG
CTTCCGCGTGGGGCTATCATTAACGGTCGTTTCGACGAAATTCATCCTGTCGATTTAACCGCCCCCGATGAGATTCAGGA
GTTTGTCACTCACTCCTGGTATACCTATGGTAACGGCAATAACGATAAGGGCCTGCATCCTTGGGATGGTCTGACCGAAC
CGCAACTGGTCATGGGCGAACACTATAAAGGCACAAAAACCTTTATCGAACAGGTGGATGAGTCCGCTAAATATTCATGG
ATTAAATCGCCGCGCTGGAAGGGACATGCGATGGAGGTAGGTCCGCTGGCGCGTTATCTGATTGGATATCATCAAAATAA
ACCGGAATTTAAAGAACCCGTGGATCAACTGCTTAGTGTATTAAAGCTGCCTAAAGAAGCGCTCTTTTCTACATTAGGAC
GTACTGCCGCGCGTGCGCTGGAATCCGTATGGGCAGGCAATACGCTGCAATATTTCTTCGATCGTCTGATGCGTAACCTC
AAGTCCGGCGATACGGCGACGGCAAACGTCACGCTCTGGGAACCCGATACCTGGCCTACATCGGCAAAAGGCGTTGGTTT
CAGCGAGGCGCCGCGCGGCGCGCTGGGGCACTGGATAAAAATTGAAAATCAGAAAATCGACAGCTATCAGTGTGTGGTAC
CGACAACCTGGAATGCCGGGCCACGTGACGATAAAGGTCAAATTGGCGCGTACGAAGCGGCGTTAATGGGGACTAAACTC
GCCGTTCCCGATCAACCATTGGAGATTTTACGTACTCTGCACAGCTTCGATCCCTGCCTGGCCTGCTCCACCCATGTTAT
TGATAATCATGGAGGGGAGCTGGTCAGGGTTCAGGTACGATGA

Upstream 100 bases:

>100_bases
GCGCTGCCGCCATCGCTCACGCCACGGTGAGCGCCATAAAACATGCCCGCAATAAAAACAACACGTCATCAGAAAACGCT
CCGGAAGAGAAAAAATAATT

Downstream 100 bases:

>100_bases
CGACGATTCGGAGGTCTCAATGCATCTCAAAGAAATAACCAGCCAGGGGTACTATATCTATGAAGCGCCGGTCCGTTTAT
GGCACTGGATCACGGCGTTA

Product: [Ni/Fe] hydrogenase, large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MAYPYQTQGFTLDNSGRRIVVDPVTRIEGHMRCEVNIDSNNVITNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCT
GTHALTSIRAVENALGIAIPDNANCIRNMMQATLHVHDHLVHFYHLHALDWVDVVAALKADPHQTSAIAQSLSAWPLSSP
GYFRDLQNRLKRFIESGQLGPFRNGYWGHPAMKLPPEANLLAVAHYLEALDFQKEIVKIHTVFGGKNPHPNWLVGGVPCA
INLDETGAVGAVNMERLNLVSSIIQKARQFCEQVYLPDVLLIASYYKDWAKIGGGLSSMNLLAYGEFPDNPNDYSASNLL
LPRGAIINGRFDEIHPVDLTAPDEIQEFVTHSWYTYGNGNNDKGLHPWDGLTEPQLVMGEHYKGTKTFIEQVDESAKYSW
IKSPRWKGHAMEVGPLARYLIGYHQNKPEFKEPVDQLLSVLKLPKEALFSTLGRTAARALESVWAGNTLQYFFDRLMRNL
KSGDTATANVTLWEPDTWPTSAKGVGFSEAPRGALGHWIKIENQKIDSYQCVVPTTWNAGPRDDKGQIGAYEAALMGTKL
AVPDQPLEILRTLHSFDPCLACSTHVIDNHGGELVRVQVR

Sequences:

>Translated_600_residues
MAYPYQTQGFTLDNSGRRIVVDPVTRIEGHMRCEVNIDSNNVITNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCT
GTHALTSIRAVENALGIAIPDNANCIRNMMQATLHVHDHLVHFYHLHALDWVDVVAALKADPHQTSAIAQSLSAWPLSSP
GYFRDLQNRLKRFIESGQLGPFRNGYWGHPAMKLPPEANLLAVAHYLEALDFQKEIVKIHTVFGGKNPHPNWLVGGVPCA
INLDETGAVGAVNMERLNLVSSIIQKARQFCEQVYLPDVLLIASYYKDWAKIGGGLSSMNLLAYGEFPDNPNDYSASNLL
LPRGAIINGRFDEIHPVDLTAPDEIQEFVTHSWYTYGNGNNDKGLHPWDGLTEPQLVMGEHYKGTKTFIEQVDESAKYSW
IKSPRWKGHAMEVGPLARYLIGYHQNKPEFKEPVDQLLSVLKLPKEALFSTLGRTAARALESVWAGNTLQYFFDRLMRNL
KSGDTATANVTLWEPDTWPTSAKGVGFSEAPRGALGHWIKIENQKIDSYQCVVPTTWNAGPRDDKGQIGAYEAALMGTKL
AVPDQPLEILRTLHSFDPCLACSTHVIDNHGGELVRVQVR
>Mature_599_residues
AYPYQTQGFTLDNSGRRIVVDPVTRIEGHMRCEVNIDSNNVITNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTG
THALTSIRAVENALGIAIPDNANCIRNMMQATLHVHDHLVHFYHLHALDWVDVVAALKADPHQTSAIAQSLSAWPLSSPG
YFRDLQNRLKRFIESGQLGPFRNGYWGHPAMKLPPEANLLAVAHYLEALDFQKEIVKIHTVFGGKNPHPNWLVGGVPCAI
NLDETGAVGAVNMERLNLVSSIIQKARQFCEQVYLPDVLLIASYYKDWAKIGGGLSSMNLLAYGEFPDNPNDYSASNLLL
PRGAIINGRFDEIHPVDLTAPDEIQEFVTHSWYTYGNGNNDKGLHPWDGLTEPQLVMGEHYKGTKTFIEQVDESAKYSWI
KSPRWKGHAMEVGPLARYLIGYHQNKPEFKEPVDQLLSVLKLPKEALFSTLGRTAARALESVWAGNTLQYFFDRLMRNLK
SGDTATANVTLWEPDTWPTSAKGVGFSEAPRGALGHWIKIENQKIDSYQCVVPTTWNAGPRDDKGQIGAYEAALMGTKLA
VPDQPLEILRTLHSFDPCLACSTHVIDNHGGELVRVQVR

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=600, Percent_Identity=66.3333333333333, Blast_Score=853, Evalue=0.0,
Organism=Escherichia coli, GI1789368, Length=596, Percent_Identity=44.7986577181208, Blast_Score=499, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 66806; Mature: 66674

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYPYQTQGFTLDNSGRRIVVDPVTRIEGHMRCEVNIDSNNVITNAVSTGTMWRGLEVIL
CCCCCCCCCEEEECCCCEEEECCHHHCCCCEEEEEEECCCCEEEECCCCCHHHHCHHHEE
KGRDPRDAWAFVERICGVCTGTHALTSIRAVENALGIAIPDNANCIRNMMQATLHVHDHL
ECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHH
VHFYHLHALDWVDVVAALKADPHQTSAIAQSLSAWPLSSPGYFRDLQNRLKRFIESGQLG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC
PFRNGYWGHPAMKLPPEANLLAVAHYLEALDFQKEIVKIHTVFGGKNPHPNWLVGGVPCA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCCEEE
INLDETGAVGAVNMERLNLVSSIIQKARQFCEQVYLPDVLLIASYYKDWAKIGGGLSSMN
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCE
LLAYGEFPDNPNDYSASNLLLPRGAIINGRFDEIHPVDLTAPDEIQEFVTHSWYTYGNGN
EEEEECCCCCCCCCCCCCEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHCCEEECCCC
NDKGLHPWDGLTEPQLVMGEHYKGTKTFIEQVDESAKYSWIKSPRWKGHAMEVGPLARYL
CCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHH
IGYHQNKPEFKEPVDQLLSVLKLPKEALFSTLGRTAARALESVWAGNTLQYFFDRLMRNL
HHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KSGDTATANVTLWEPDTWPTSAKGVGFSEAPRGALGHWIKIENQKIDSYQCVVPTTWNAG
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCC
PRDDKGQIGAYEAALMGTKLAVPDQPLEILRTLHSFDPCLACSTHVIDNHGGELVRVQVR
CCCCCCCCCHHHHHHHCCEECCCCCHHHHHHHHHCCCHHHHHHHHHEECCCCCEEEEEEC
>Mature Secondary Structure 
AYPYQTQGFTLDNSGRRIVVDPVTRIEGHMRCEVNIDSNNVITNAVSTGTMWRGLEVIL
CCCCCCCCEEEECCCCEEEECCHHHCCCCEEEEEEECCCCEEEECCCCCHHHHCHHHEE
KGRDPRDAWAFVERICGVCTGTHALTSIRAVENALGIAIPDNANCIRNMMQATLHVHDHL
ECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHH
VHFYHLHALDWVDVVAALKADPHQTSAIAQSLSAWPLSSPGYFRDLQNRLKRFIESGQLG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC
PFRNGYWGHPAMKLPPEANLLAVAHYLEALDFQKEIVKIHTVFGGKNPHPNWLVGGVPCA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCCEEE
INLDETGAVGAVNMERLNLVSSIIQKARQFCEQVYLPDVLLIASYYKDWAKIGGGLSSMN
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCE
LLAYGEFPDNPNDYSASNLLLPRGAIINGRFDEIHPVDLTAPDEIQEFVTHSWYTYGNGN
EEEEECCCCCCCCCCCCCEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHCCEEECCCC
NDKGLHPWDGLTEPQLVMGEHYKGTKTFIEQVDESAKYSWIKSPRWKGHAMEVGPLARYL
CCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHH
IGYHQNKPEFKEPVDQLLSVLKLPKEALFSTLGRTAARALESVWAGNTLQYFFDRLMRNL
HHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KSGDTATANVTLWEPDTWPTSAKGVGFSEAPRGALGHWIKIENQKIDSYQCVVPTTWNAG
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCC
PRDDKGQIGAYEAALMGTKLAVPDQPLEILRTLHSFDPCLACSTHVIDNHGGELVRVQVR
CCCCCCCCCHHHHHHHCCEECCCCCHHHHHHHHHCCCHHHHHHHHHEECCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3054886; 12597275; 8163174 [H]